LOCUS RC211284 7761 bp DNA circular SYN DEFINITION AAK1 (Myc-DDK-tagged)-Human AP2 associated kinase 1 (AAK1) ACCESSION NM_014911 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 3918..4009 /label="Myc-DDK" misc_feature 4039..4624 /label="PolyA signal" misc_feature 4096..4115 /label="Restriction Enzyme" misc_feature 4900..5572 /label="ColE1" misc_feature 6002..6796 /label="Kan/Neo" misc_feature 6831..7133 /label="SV40 ori" misc_feature 7219..7696 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 3900..3906 /label="Restriction Enzyme" misc_feature 1020..3900 /label="AAK1" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GAAGAAGTTT TTCGACTCCC GGCGAGAGCA GGGCGGCTCT GGCCTGGGCT 1081 CCGGCTCCAG CGGAGGAGGG GGCAGCACCT CGGGCCTGGG CAGTGGCTAC ATCGGAAGAG 1141 TCTTCGGCAT CGGGCGACAG CAGGTCACAG TGGACGAGGT GTTGGCGGAA GGTGGATTTG 1201 CTATTGTATT TCTGGTGAGG ACAAGCAATG GGATGAAATG TGCCTTGAAA CGCATGTTTG 1261 TCAACAATGA GCATGATCTC CAGGTGTGCA AGAGAGAAAT CCAGATAATG AGGGATCTTT 1321 CAGGGCACAA GAATATTGTG GGTTACATTG ATTCTAGTAT CAACAACGTG AGTAGCGGTG 1381 ATGTATGGGA AGTGCTCATT CTGATGGACT TTTGTAGAGG TGGCCAGGTG GTAAACCTGA 1441 TGAACCAGCG CCTGCAAACA GGCTTTACAG AGAATGAAGT GCTCCAGATA TTTTGTGATA 1501 CCTGTGAAGC TGTTGCCCGC CTGCATCAGT GCAAAACTCC TATTATCCAC CGGGACCTGA 1561 AGGTTGAAAA CATCCTCTTG CATGACCGAG GCCACTATGT CCTGTGTGAC TTTGGAAGCG 1621 CCACCAACAA ATTCCAGAAT CCACAAACTG AGGGAGTCAA TGCAGTAGAA GATGAGATTA 1681 AGAAATACAC AACGCTGTCC TATCGAGCAC CAGAAATGGT CAACCTGTAC AGTGGCAAAA 1741 TCATCACTAC GAAGGCAGAC ATTTGGGCTC TTGGATGTTT GTTGTATAAA TTATGCTACT 1801 TCACTTTGCC ATTTGGGGAA AGTCAGGTGG CAATTTGTGA TGGAAACTTC ACAATTCCTG 1861 ATAATTCTCG ATATTCTCAA GACATGCACT GCCTAATTAG GTATATGTTG GAACCAGACC 1921 CTGACAAAAG GCCGGATATT TACCAGGTGT CCTACTTCTC ATTTAAGCTA CTCAAGAAAG 1981 AGTGCCCAAT TCCAAATGTA CAGAACTCTC CCATTCCTGC AAAGCTTCCT GAACCAGTGA 2041 AAGCCAGTGA GGCAGCTGCA AAAAAGACCC AGCCAAAGGC CAGACTGACA GATCCCATTC 2101 CCACCACAGA GACTTCAATT GCACCCCGCC AGAGGCCTAA AGCTGGGCAG ACTCAGCCGA 2161 ACCCAGGAAT CCTTCCCATC CAGCCAGCGC TGACACCCCG GAAGAGGGCC ACTGTTCAGC 2221 CCCCACCTCA GGCTGCAGGA TCCAGCAATC AGCCTGGCCT TTTAGCCAGT GTTCCCCAAC 2281 CAAAACCCCA AGCCCCACCC AGCCAGCCTC TGCCGCAAAC TCAGGCCAAG CAGCCACAGG 2341 CTCCTCCCAC TCCACAGCAG ACGCCTTCTA CTCAGGCCCA GGGTCTGCCC GCTCAGGCCC 2401 AGGCCACACC CCAGCACCAG CAGCAACTCT TCCTCAAGCA GCAACAGCAG CAGCAACAGC 2461 CACCGCCAGC ACAGCAGCAG CCGGCAGGCA CGTTTTACCA GCAGCAGCAG GCCCAGACTC 2521 AGCAGTTTCA GGCAGTACAT CCAGCAACCC AGCAACCAGC AATTGCTCAG TTCCCTGTGG 2581 TGTCCCAAGG AGGCTCTCAA CAGCAGCTAA TGCAGAATTT CTACCAGCAG CAGCAGCAGC 2641 AGCAACAACA GCAACAGCAA CAGCTGGCCA CAGCCCTGCA TCAACAACAG CTGATGACTC 2701 AGCAGGCTGC CTTGCAGCAA AAGCCCACTA TGGCAGCAGG ACAGCAGCCC CAGCCACAGC 2761 CAGCTGCAGC CCCACAGCCA GCCCCTGCCC AGGAACCAGC GATTCAAGCC CCAGTAAGAC 2821 AACAGCCAAA GGTTCAGACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA GTTGGATCTC 2881 TCACTCCACC CTCATCCCCC AAAACCCAAC GTGCTGGGCA CAGGCGTATT CTCAGTGACG 2941 TAACCCACAG TGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG CTCCAGGCAG 3001 CTGCAGCTGA GGCCAGTCTC AATAAGTCCA AGTCTGCAAC CACCACTCCA TCAGGCTCTC 3061 CTCGGACCTC TCAACAAAAC GTTTATAATC CTTCAGAAGG GTCTACGTGG AATCCCTTTG 3121 ATGACGATAA TTTCTCCAAA CTCACAGCTG AAGAACTGCT AAACAAGGAC TTTGCCAAGC 3181 TTGGGGAAGG CAAACATCCC GAGAAGCTTG GAGGCTCAGC TGAGAGTTTG ATCCCAGGCT 3241 TTCAATCAAC CCAAGGTGAT GCTTTTGCTA CGACCTCATT TTCTGCTGGA ACTGCTGAAA 3301 AAAGGAAGGG TGGGCAGACT GTGGACTCTG GCCTCCCGCT TCTAAGCGTG TCTGATCCTT 3361 TCATTCCTCT TCAAGTACCT GATGCACCAG AAAAACTAAT TGAGGGACTC AAATCTCCTG 3421 ACACTTCTCT TCTGCTCCCT GACCTCTTGC CTATGACAGA TCCTTTTGGT AGCACTTCTG 3481 ATGCTGTAAT TGAAAAAGCT GATGTTGCTG TTGAGAGTCT CATACCAGGA CTGGAGCCCC 3541 CAGTTCCCCA GCGCCTCCCA TCTCAGACGG AATCTGTGAC CTCGAATCGC ACAGATTCTC 3601 TCACCGGGGA AGATTCCCTG CTTGATTGCT CTCTGCTCTC TAACCCTACT ACTGACCTTC 3661 TGGAAGAGTT TGCCCCCACA GCAATCTCTG CTCCAGTCCA TAAAGCTGCA GAAGATAGTA 3721 ATCTCATCTC AGGTTTTGAT GTCCCTGAGG GCTCGGACAA GGTGGCTGAA GATGAGTTTG 3781 ACCCTATTCC TGTATTGATA ACCAAAAACC CACAAGGTGG GCACTCTAGA AACAGCAGTG 3841 GGAGCTCTGA GTCCAGTCTT CCCAACCTAG CCAGGTCTTT ACTGCTGGTG GATCAGCTCA 3901 TAGACCTGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 3961 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 4021 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 4081 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 4141 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 4201 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 4261 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 4321 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 4381 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 4441 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 4501 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 4561 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 4621 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 4681 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 4741 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 4801 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 4861 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 4921 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 4981 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 5041 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 5101 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 5161 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 5221 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 5281 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 5341 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 5401 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 5461 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 5521 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 5581 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 5641 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 5701 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 5761 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 5821 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 5881 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 5941 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 6001 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 6061 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 6121 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 6181 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 6241 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 6301 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 6361 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 6421 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 6481 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 6541 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 6601 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 6661 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 6721 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 6781 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 6841 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 6901 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 6961 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 7021 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 7081 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 7141 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 7201 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 7261 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 7321 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 7381 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 7441 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 7501 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 7561 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 7621 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 7681 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 7741 CAAAAATTTA ACGCGAATTT T //