LOCUS       RC211229                8115 bp    DNA     circular  SYN 
DEFINITION  CASR (Myc-DDK-tagged)-Human calcium-sensing receptor (CASR), transcript variant 2
ACCESSION   NM_000388
VERSION     NM_000388.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4272..4363 
                     /label="Myc-DDK"  
     misc_feature    4393..4978 
                     /label="PolyA signal"  
     misc_feature    4450..4469 
                     /label="Restriction Enzyme"  
     misc_feature    5254..5926 
                     /label="ColE1"  
     misc_feature    6356..7150 
                     /label="Kan/Neo"  
     misc_feature    7185..7487 
                     /label="SV40 ori"  
     misc_feature    7573..8050 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4254..4260 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4254 
                     /label="CASR"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCATTTTAT AGCTGCTGCT GGGTCCTCTT GGCACTCACC TGGCACACCT 
     1081 CTGCCTACGG GCCAGACCAG CGAGCCCAAA AGAAGGGGGA CATTATCCTT GGGGGGCTCT 
     1141 TTCCTATTCA TTTTGGAGTA GCAGCTAAAG ATCAAGATCT CAAATCAAGG CCGGAGTCTG 
     1201 TGGAATGTAT CAGGTATAAT TTCCGTGGGT TTCGCTGGTT ACAGGCTATG ATATTTGCCA 
     1261 TAGAGGAGAT AAACAGCAGC CCAGCCCTTC TTCCCAACTT GACGCTGGGA TACAGGATAT 
     1321 TTGACACTTG CAACACCGTT TCTAAGGCCT TGGAAGCCAC CCTGAGTTTT GTTGCTCAAA 
     1381 ACAAAATTGA TTCTTTGAAC CTTGATGAGT TCTGCAACTG CTCAGAGCAC ATTCCCTCTA 
     1441 CGATTGCTGT GGTGGGAGCA ACTGGCTCAG GCGTCTCCAC GGCAGTGGCA AATCTGCTGG 
     1501 GGCTCTTCTA CATTCCCCAG GTCAGTTATG CCTCCTCCAG CAGACTCCTC AGCAACAAGA 
     1561 ATCAATTCAA GTCTTTCCTC CGAACCATCC CCAATGATGA GCACCAGGCC ACTGCCATGG 
     1621 CAGACATCAT CGAGTATTTC CGCTGGAACT GGGTGGGCAC AATTGCAGCT GATGACGACT 
     1681 ATGGGCGGCC GGGGATTGAG AAATTCCGAG AGGAAGCTGA GGAAAGGGAT ATCTGCATCG 
     1741 ACTTCAGTGA ACTCATCTCC CAGTACTCTG ATGAGGAAGA GATCCAGCAT GTGGTAGAGG 
     1801 TGATTCAAAA TTCCACGGCC AAAGTCATCG TGGTTTTCTC CAGTGGCCCA GATCTTGAGC 
     1861 CCCTCATCAA GGAGATTGTC CGGCGCAATA TCACGGGCAA GATCTGGCTG GCCAGCGAGG 
     1921 CCTGGGCCAG CTCCTCCCTG ATCGCCATGC CTCAGTACTT CCACGTGGTT GGCGGCACCA 
     1981 TTGGATTCGC TCTGAAGGCT GGGCAGATCC CAGGCTTCCG GGAATTCCTG AAGAAGGTCC 
     2041 ATCCCAGGAA GTCTGTCCAC AATGGTTTTG CCAAGGAGTT TTGGGAAGAA ACATTTAACT 
     2101 GCCACCTCCA AGAAGGTGCA AAAGGACCTT TACCTGTGGA CACCTTTCTG AGAGGTCACG 
     2161 AAGAAAGTGG CGACAGGTTT AGCAACAGCT CGACAGCCTT CCGACCCCTC TGTACAGGGG 
     2221 ATGAGAACAT CAGCAGTGTC GAGACCCCTT ACATAGATTA CACGCATTTA CGGATATCCT 
     2281 ACAATGTGTA CTTAGCAGTC TACTCCATTG CCCACGCCTT GCAAGATATA TATACCTGCT 
     2341 TACCTGGGAG AGGGCTCTTC ACCAATGGCT CCTGTGCAGA CATCAAGAAA GTTGAGGCGT 
     2401 GGCAGGTCCT GAAGCACCTA CGGCATCTAA ACTTTACAAA CAATATGGGG GAGCAGGTGA 
     2461 CCTTTGATGA GTGTGGTGAC CTGGTGGGGA ACTATTCCAT CATCAACTGG CACCTCTCCC 
     2521 CAGAGGATGG CTCCATCGTG TTTAAGGAAG TCGGGTATTA CAACGTCTAT GCCAAGAAGG 
     2581 GAGAAAGACT CTTCATCAAC GAGGAGAAAA TCCTGTGGAG TGGGTTCTCC AGGGAGGTGC 
     2641 CCTTCTCCAA CTGCAGCCGA GACTGCCTGG CAGGGACCAG GAAAGGGATC ATTGAGGGGG 
     2701 AGCCCACCTG CTGCTTTGAG TGTGTGGAGT GTCCTGATGG GGAGTATAGT GATGAGACAG 
     2761 ATGCCAGTGC CTGTAACAAG TGCCCAGATG ACTTCTGGTC CAATGAGAAC CACACCTCCT 
     2821 GCATTGCCAA GGAGATCGAG TTTCTGTCGT GGACGGAGCC CTTTGGGATC GCACTCACCC 
     2881 TCTTTGCCGT GCTGGGCATT TTCCTGACAG CCTTTGTGCT GGGTGTGTTT ATCAAGTTCC 
     2941 GCAACACACC CATTGTCAAG GCCACCAACC GAGAGCTCTC CTACCTCCTC CTCTTCTCCC 
     3001 TGCTCTGCTG CTTCTCCAGC TCCCTGTTCT TCATCGGGGA GCCCCAGGAC TGGACGTGCC 
     3061 GCCTGCGCCA GCCGGCCTTT GGCATCAGCT TCGTGCTCTG CATCTCATGC ATCCTGGTGA 
     3121 AAACCAACCG TGTCCTCCTG GTGTTTGAGG CCAAGATCCC CACCAGCTTC CACCGCAAGT 
     3181 GGTGGGGGCT CAACCTGCAG TTCCTGCTGG TTTTCCTCTG CACCTTCATG CAGATTGTCA 
     3241 TCTGTGTGAT CTGGCTCTAC ACCGCGCCCC CCTCAAGCTA CCGCAACCAG GAGCTGGAGG 
     3301 ATGAGATCAT CTTCATCACG TGCCACGAGG GCTCCCTCAT GGCCCTGGGC TTCCTGATCG 
     3361 GCTACACCTG CCTGCTGGCT GCCATCTGCT TCTTCTTTGC CTTCAAGTCC CGGAAGCTGC 
     3421 CGGAGAACTT CAATGAAGCC AAGTTCATCA CCTTCAGCAT GCTCATCTTC TTCATCGTCT 
     3481 GGATCTCCTT CATTCCAGCC TATGCCAGCA CCTATGGCAA GTTTGTCTCT GCCGTAGAGG 
     3541 TGATTGCCAT CCTGGCAGCC AGCTTTGGCT TGCTGGCGTG CATCTTCTTC AACAAGATCT 
     3601 ACATCATTCT CTTCAAGCCA TCCCGCAACA CCATCGAGGA GGTGCGTTGC AGCACCGCAG 
     3661 CTCACGCTTT CAAGGTGGCT GCCCGGGCCA CGCTGCGCCG CAGCAACGTC TCCCGCAAGC 
     3721 GGTCCAGCAG CCTTGGAGGC TCCACGGGAT CCACCCCCTC CTCCTCCATC AGCAGCAAGA 
     3781 GCAACAGCGA AGACCCATTC CCACAGCCCG AGAGGCAGAA GCAGCAGCAG CCGCTGGCCC 
     3841 TAACCCAGCA AGAGCAGCAG CAGCAGCCCC TGACCCTCCC ACAGCAGCAA CGATCTCAGC 
     3901 AGCAGCCCAG ATGCAAGCAG AAGGTCATCT TTGGCAGCGG CACGGTCACC TTCTCACTGA 
     3961 GCTTTGATGA GCCTCAGAAG AACGCCATGG CCCACAGGAA TTCTACGCAC CAGAACTCCC 
     4021 TGGAGGCCCA GAAAAGCAGC GATACGCTGA CCCGACACCA GCCATTACTC CCGCTGCAGT 
     4081 GCGGGGAAAC GGACTTAGAT CTGACCGTCC AGGAAACAGG TCTGCAAGGA CCTGTGGGTG 
     4141 GAGACCAGCG GCCAGAGGTG GAGGACCCTG AAGAGTTGTC CCCAGCACTT GTAGTGTCCA 
     4201 GTTCACAGAG CTTTGTCATC AGTGGTGGAG GCAGCACTGT TACAGAAAAC GTAGTGAATT 
     4261 CAACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4321 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4381 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4441 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4501 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4561 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4621 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4681 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4741 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4801 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4861 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4921 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4981 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5041 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5101 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5161 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5221 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5281 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5341 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5401 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5461 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5521 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5581 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5641 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5701 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5761 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5821 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5881 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5941 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6001 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6061 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6121 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6181 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6241 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6301 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6361 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6421 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6481 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6541 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6601 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6661 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6721 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6781 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6841 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6901 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6961 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7021 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7081 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7141 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7201 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7261 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7321 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7381 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7441 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7501 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7561 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7621 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7681 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7741 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7801 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7861 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7921 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7981 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8041 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8101 TTTAACGCGA ATTTT
//