LOCUS       RC211186L1                9507 bp    DNA     circular  SYN 
DEFINITION  KLB (Myc-DDK-tagged)-Human klotho beta (KLB)
ACCESSION   NM_175737
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7798..7978 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8706..9365 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    8137..8705 
                     /label="ColE1"  
     misc_feature    6673..6693 
                     /label="LR50 primer"  
     misc_feature    6470..6541 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6682..7273 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6452..6458 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6452 
                     /label="KLB"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAAGCCAGGC TGTGCGGCAG GATCTCCAGG 
     3361 GAATGAATGG ATTTTCTTCA GCACTGATGA AATAACCACA CGCTATAGGA ATACAATGTC 
     3421 CAACGGGGGA TTGCAAAGAT CTGTCATCCT GTCAGCACTT ATTCTGCTAC GAGCTGTTAC 
     3481 TGGATTCTCT GGAGATGGAA GAGCTATATG GTCTAAAAAT CCTAATTTTA CTCCGGTAAA 
     3541 TGAAAGTCAG CTGTTTCTCT ATGACACTTT CCCTAAAAAC TTTTTCTGGG GTATTGGGAC 
     3601 TGGAGCATTG CAAGTGGAAG GGAGTTGGAA GAAGGATGGA AAAGGACCTT CTATATGGGA 
     3661 TCATTTCATC CACACACACC TTAAAAATGT CAGCAGCACG AATGGTTCCA GTGACAGTTA 
     3721 TATTTTTCTG GAAAAAGACT TATCAGCCCT GGATTTTATA GGAGTTTCTT TTTATCAATT 
     3781 TTCAATTTCC TGGCCAAGGC TTTTCCCCGA TGGAATAGTA ACAGTTGCCA ACGCAAAAGG 
     3841 TCTGCAGTAC TACAGTACTC TTCTGGACGC TCTAGTGCTT AGAAACATTG AACCTATAGT 
     3901 TACTTTATAC CACTGGGATT TGCCTTTGGC ACTACAAGAA AAATATGGGG GGTGGAAAAA 
     3961 TGATACCATA ATAGATATCT TCAATGACTA TGCCACATAC TGTTTCCAGA TGTTTGGGGA 
     4021 CCGTGTCAAA TATTGGATTA CAATTCACAA CCCATATCTA GTGGCTTGGC ATGGGTATGG 
     4081 GACAGGTATG CATGCCCCTG GAGAGAAGGG AAATTTAGCA GCTGTCTACA CTGTGGGACA 
     4141 CAACTTGATC AAGGCTCACT CGAAAGTTTG GCATAACTAC AACACACATT TCCGCCCACA 
     4201 TCAGAAGGGT TGGTTATCGA TCACGTTGGG ATCTCATTGG ATCGAGCCAA ACCGGTCGGA 
     4261 AAACACGATG GATATATTCA AATGTCAACA ATCCATGGTT TCTGTGCTTG GATGGTTTGC 
     4321 CAACCCTATC CATGGGGATG GCGACTATCC AGAGGGGATG AGAAAGAAGT TGTTCTCCGT 
     4381 TCTACCCATT TTCTCTGAAG CAGAGAAGCA TGAGATGAGA GGCACAGCTG ATTTCTTTGC 
     4441 CTTTTCTTTT GGACCCAACA ACTTCAAGCC CCTAAACACC ATGGCTAAAA TGGGACAAAA 
     4501 TGTTTCACTT AATTTAAGAG AAGCGCTGAA CTGGATTAAA CTGGAATACA ACAACCCTCG 
     4561 AATCTTGATT GCTGAGAATG GCTGGTTCAC AGACAGTCGT GTGAAAACAG AAGACACCAC 
     4621 GGCCATCTAC ATGATGAAGA ATTTCCTCAG CCAGGTGCTT CAAGCAATAA GGTTAGATGA 
     4681 AATACGAGTG TTTGGTTATA CTGCCTGGTC TCTCCTGGAT GGCTTTGAAT GGCAGGATGC 
     4741 TTACACCATC CGCCGAGGAT TATTTTATGT GGATTTTAAC AGTAAACAGA AAGAGCGGAA 
     4801 ACCTAAGTCT TCAGCACACT ACTACAAACA GATCATACGA GAAAATGGTT TTTCTTTAAA 
     4861 AGAGTCCACG CCAGATGTGC AGGGCCAGTT TCCCTGTGAC TTCTCCTGGG GTGTCACTGA 
     4921 ATCTGTTCTT AAGCCCGAGT CTGTGGCTTC GTCCCCACAG TTCAGCGATC CTCATCTGTA 
     4981 CGTGTGGAAC GCCACTGGCA ACAGACTGTT GCACCGAGTG GAAGGGGTGA GGCTGAAAAC 
     5041 ACGACCCGCT CAATGCACAG ATTTTGTAAA CATCAAAAAA CAACTTGAGA TGTTGGCAAG 
     5101 AATGAAAGTC ACCCACTACC GGTTTGCTCT GGATTGGGCC TCGGTCCTTC CCACTGGCAA 
     5161 CCTGTCCGCG GTGAACCGAC AGGCCCTGAG GTACTACAGG TGCGTGGTCA GTGAGGGGCT 
     5221 GAAGCTTGGC ATCTCCGCGA TGGTCACCCT GTATTATCCG ACCCACGCCC ACCTAGGCCT 
     5281 CCCCGAGCCT CTGTTGCATG CCGACGGGTG GCTGAACCCA TCGACGGCCG AGGCCTTCCA 
     5341 GGCCTACGCT GGGCTGTGCT TCCAGGAGCT GGGGGACCTG GTGAAGCTCT GGATCACCAT 
     5401 CAACGAGCCT AACCGGCTAA GTGACATCTA CAACCGCTCT GGCAACGACA CCTACGGGGC 
     5461 GGCGCACAAC CTGCTGGTGG CCCACGCCCT GGCCTGGCGC CTCTACGACC GGCAGTTCAG 
     5521 GCCCTCACAG CGCGGGGCCG TGTCGCTGTC GCTGCACGCG GACTGGGCGG AACCCGCCAA 
     5581 CCCCTATGCT GACTCGCACT GGAGGGCGGC CGAGCGCTTC CTGCAGTTCG AGATCGCCTG 
     5641 GTTCGCCGAG CCGCTCTTCA AGACCGGGGA CTACCCCGCG GCCATGAGGG AATACATTGC 
     5701 CTCCAAGCAC CGACGGGGGC TTTCCAGCTC GGCCCTGCCG CGCCTCACCG AGGCCGAAAG 
     5761 GAGGCTGCTC AAGGGCACGG TCGACTTCTG CGCGCTCAAC CACTTCACCA CTAGGTTCGT 
     5821 GATGCACGAG CAGCTGGCCG GCAGCCGCTA CGACTCGGAC AGGGACATCC AGTTTCTGCA 
     5881 GGACATCACC CGCCTGAGCT CCCCCACGCG CCTGGCTGTG ATTCCCTGGG GGGTGCGCAA 
     5941 GCTGCTGCGG TGGGTCCGGA GGAACTACGG CGACATGGAC ATTTACATCA CCGCCAGTGG 
     6001 CATCGACGAC CAGGCTCTGG AGGATGACCG GCTCCGGAAG TACTACCTAG GGAAGTACCT 
     6061 TCAGGAGGTG CTGAAAGCAT ACCTGATTGA TAAAGTCAGA ATCAAAGGCT ATTATGCATT 
     6121 CAAACTGGCT GAAGAGAAAT CTAAACCCAG ATTTGGATTC TTCACATCTG ATTTTAAAGC 
     6181 TAAATCCTCA ATACAATTTT ACAACAAAGT GATCAGCAGC AGGGGCTTCC CTTTTGAGAA 
     6241 CAGTAGTTCT AGATGCAGTC AGACCCAAGA AAATACAGAG TGCACTGTCT GCTTATTCCT 
     6301 TGTGCAGAAG AAACCACTGA TATTCCTGGG TTGTTGCTTC TTCTCCACCC TGGTTCTACT 
     6361 CTTATCAATT GCCATTTTTC AAAGGCAGAA GAGAAGAAAG TTTTGGAAAG CAAAAAACTT 
     6421 ACAACACATA CCATTAAAGA AAGGCAAGAG AGTTGTTAGC ACGCGTACGC GGCCGCTCGA 
     6481 GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA 
     6541 CGACGATAAG GTTTAAACGG CCGGCCGCGG TCTGTACAAG TAGGATTCGT CGAGGGACCT 
     6601 AATAACTTCG TATAGCATAC ATTATACGAA GTTATACATG TTTAAGGGTT CCGGTTCCAC 
     6661 TAGGTACAAT TCGATATCAA GCTTATCGAT AATCAACCTC TGGATTACAA AATTTGTGAA 
     6721 AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA 
     6781 ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA 
     6841 TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG 
     6901 TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC 
     6961 CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC 
     7021 CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG 
     7081 GGGAAATCAT CGTCCTTTCC TTGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG 
     7141 ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG 
     7201 CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC 
     7261 CTTTGGGCCG CCTCCCCGCA TCGATACCGT CGACCTCGAT CGAGACCTAG AAAAACATGG 
     7321 AGCAATCACA AGTAGCAATA CAGCAGCTAC CAATGCTGAT TGTGCCTGGC TAGAAGCACA 
     7381 AGAGGAGGAG GAGGTGGGTT TTCCAGTCAC ACCTCAGGTA CCTTTAAGAC CAATGACTTA 
     7441 CAAGGCAGCT GTAGATCTTA GCCACTTTTT AAAAGAAAAG GGGGGACTGG AAGGGCTAAT 
     7501 TCACTCCCAA CGAAGACAAG ATATCCTTGA TCTGTGGATC TACCACACAC AAGGCTACTT 
     7561 CCCTGATTGG CAGAACTACA CACCAGGGCC AGGGATCAGA TATCCACTGA CCTTTGGATG 
     7621 GTGCTACAAG CTAGTACCAG TTGAGCAAGA GAAGGTAGAA GAAGCCAATG AAGGAGAGAA 
     7681 CACCCGCTTG TTACACCCTG TGAGCCTGCA TGGGATGGAT GACCCGGAGA GAGAAGTATT 
     7741 AGAGTGGAGG TTTGACAGCC GCCTAGCATT TCATCACATG GCCCGAGAGC TGCATCCGGA 
     7801 CTGTACTGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG GAGCTCTCTG GCTAACTAGG 
     7861 GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG 
     7921 TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC TTTTAGTCAG TGTGGAAAAT 
     7981 CTCTAGCAGC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     8041 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     8101 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     8161 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     8221 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     8281 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     8341 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     8401 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     8461 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     8521 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     8581 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     8641 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     8701 GATTTTGGTC ATGATTACGC CCCGCCCTGC CACTCATCGC AGTACTGTTG TAATTCATTA 
     8761 AGCATTCTGC CGACATGGAA GCCATCACAA ACGGCATGAT GAACCTGAAT CGCCAGCGGC 
     8821 ATCAGCACCT TGTCGCCTTG CGTATAATAT TTGCCCATGG TGAAAACGGG GGCGAAGAAG 
     8881 TTGTCCATAT TGGCCACGTT TAAATCAAAA CTGGTGAAAC TCACCCAGGG ATTGGCTGAG 
     8941 ACGAAAAACA TATTCTCAAT AAACCCTTTA GGGAAATAGG CCAGGTTTTC ACCGTAACAC 
     9001 GCCACATCTT GCGAATATAT GTGTAGAAAC TGCCGGAAAT CGTCGTGGTA TTCACTCCAG 
     9061 AGCGATGAAA ACGTTTCAGT TTGCTCATGG AAAACGGTGT AACAAGGGTG AACACTATCC 
     9121 CATATCACCA GCTCACCGTC TTTCATTGCC ATACGGAACT CCGGATGAGC ATTCATCAGG 
     9181 CGGGCAAGAA TGTGAATAAA GGCCGGATAA AACTTGTGCT TATTTTTCTT TACGGTCTTT 
     9241 AAAAAGGCCG TAATATCCAG CTGAACGGTC TGGTTATAGG TACATTGAGC AACTGACTGA 
     9301 AATGCCTCAA AATGTTCTTT ACGATGCCAT TGGGATATAT CAACGGTGGT ATATCCAGTG 
     9361 ATTTTTTTCT CCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     9421 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTCCCGCGC 
     9481 ACATTTCCCC GAAAAGTGCC ACCTGAC
//