LOCUS       RC211165                7269 bp    DNA     circular  SYN 
DEFINITION  PCDHB3 (Myc-DDK-tagged)-Human protocadherin beta 3 (PCDHB3)
ACCESSION   NM_018937
VERSION     NM_018937.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3426..3517 
                     /label="Myc-DDK"  
     misc_feature    3547..4132 
                     /label="PolyA signal"  
     misc_feature    3604..3623 
                     /label="Restriction Enzyme"  
     misc_feature    4408..5080 
                     /label="ColE1"  
     misc_feature    5510..6304 
                     /label="Kan/Neo"  
     misc_feature    6339..6641 
                     /label="SV40 ori"  
     misc_feature    6727..7204 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3408..3414 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3408 
                     /label="PCDHB3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCGGGA GGAGAGCGAT TTCTTAGACA AAGGCAAGTC TTGCTTCTCT 
     1081 TTGTTTTTCT GGGAGGGTCT CTGGCTGGGT CCGAGTCAAG ACGCTATTCT GTGGCTGAGG 
     1141 AAAAAGAGAG GGGCTTTTTA ATAGCCAACC TAGCAAAGGA TCTGGGACTA AGGGTAGAGG 
     1201 AACTGGCCGC GAGGGGGGCC CAAGTTGTGT CCAAAGGGAA CAAACAGCAT TTTCAGCTCA 
     1261 GTCATCAGAC AGGTGATTTG CTCCTGAATG AGAAATTGGA CCGGGAGGAG CTATGCGGCC 
     1321 CCACAGAACC ATGCATACTA CATTTTCAGA TATTACTGCA AAACCCTTTG CAGTTCGTTA 
     1381 CAAACGAGCT CCGTATCATA GATGTAAATG ACCATTCTCC GGTATTCTTT GAAAATGAAA 
     1441 TGCATCTGAA AATCCTAGAA AGCACTCTGC CAGGAACAGT AATTCCTTTG GGAAATGCTG 
     1501 AGGACTTGGA TGTGGGAAGA AACAGCCTCC AAAACTACAC TATCACTCCG AATTCCCACT 
     1561 TCCACGTACT CACTCGCAGT CGTAGGGACG GAAGGAAGTA CCCGGAACTA GTACTGGATA 
     1621 AAGCGCTCGA TCGGGAGGAG CAGCCGGAAC TCAGCTTAAC GCTCACCGCG CTGGACGGCG 
     1681 GCTCTCCCCC TCGGTCTGGG ACAGCCCAGA TAAACATCCA GGTCTTAGAT ATAAACGACA 
     1741 ATGCACCAGA ATTTGCACAG CCGCTCTATG AGGTTGCAGT TCTAGAGAAT ACCCCCGTTA 
     1801 ACTCTGTCAT TGTCACTGTC TCGGCTTCTG ACTTAGATAC AGGAAGTTTT GGGACAATAT 
     1861 CATATGCATT TTTTCATGCT TCTGAAGAAA TTCGCAAAAC TTTTCAGCTA AATCCAATTA 
     1921 CTGGTGATAT GCAACTGGTC AAATATTTGA ATTTTGAAGC GATTAATAGT TATGAAGTCG 
     1981 ACATCGAGGC CAAGGATGGC GGAGGCCTAT CCGGAAAGTC TACAGTCATA GTCCAGGTGG 
     2041 TTGATGTCAA CGACAACCCA CCGGAACTGA CCTTGTCTTC AGTAAACAGC CCTATTCCTG 
     2101 AGAACTCGGG AGAGACTGTA CTGGCTGTTT TCAGTGTTTC TGATCTAGAC TCTGGAGACA 
     2161 ACGGAAGAGT GATGTGTTCC ATTGAGAACA ATCTCCCCTT CTTCCTGAAA CCATCTGTAG 
     2221 AGAATTTTTA CACCCTAGTG TCAGAAGGCG CGCTGGACAG AGAGACCAGA TCCGAGTACA 
     2281 ACATTACCAT CACTATCACT GACCTGGGGA CACCCAGGCT GAAAACCAAG TACAACATAA 
     2341 CCGTGCTGGT CTCCGACGTC AATGACAACG CCCCCGCCTT CACCCAAACC TCCTACACCC 
     2401 TGTTCGTCCG CGAGAACAAC AGCCCCGCCC TGCACATCGG CAGTGTCAGC GCCACAGACA 
     2461 GAGACTCAGG CACCAACGCC CAGGTAACCT ACTCGCTGCT GCCGCCCCAG GACCCGCACC 
     2521 TGCCCCTCTC TTCCCTGGTC TCCATCAACG CGGACAACGG CCACCTGTTT GCCCTCAGGT 
     2581 CGCTGGACTA CGAGGCCCTG CAGGCGTTCG AGTTCCGCGT GGGCGCCGCA GACCGCGGCT 
     2641 CCCCGGCTTT GAGCAGCGAG GCGCTGGTGC GCGTGCTGGT GCTGGACGCC AACGACAACT 
     2701 CGCCCTTTGT GCTGTACCCG CTGCAGAACG GCTCCGCGCC CTGCACCGAG CTGGTGCCCC 
     2761 GGGCGGCTGA GCCGGGCTAC CTGGTGACCA AGGTGGTGGC GGTGGACAGC GACTCGGGCC 
     2821 AGAACGCCTG GCTGTCGTAC CAGCTGCTCA AGGCCACGGA GCCCGGGCTG TTCGGCGTGT 
     2881 GGGCGCACAA TGGCGAGGTG CGCACCGCCA GGCTGCTGAG CGAGCGCGAC GCGGCCAAGC 
     2941 ACAGGCTGGT GGTGCTGGTC AAGGACAATG GCGAGCCTCC GCGCTCGGCC ACCGCCACGC 
     3001 TGCATGTGCT CCTGGTGGAC GGCTTCTCCC AGCCCTACCT GCCTCTCCCG GAGGCGGCAC 
     3061 CGGCCCAGGC CCAGGCCGAC TTGCTCACCG TCTACCTGGT GGTGGCGTTG GCCTCGGTGT 
     3121 CTTCGCTCTT CCTCTTCTCG GTGCTCCTGT TCGTGGCGGT GCGGCTGTGC AGGAGGAGCA 
     3181 GGGCGGCCTC GGTGGGTCGC TGCTCGGTGC CCGAGGGCCC CTTTCCAGGG CAGATGGTGG 
     3241 ACGTGAGCGG CACCGGGACC CTGTCCCAGA GCTACCAATA CGAGGTGTGT CTGACTGGAG 
     3301 GCTCCGGGAC AAATGAGTTC AAGTTCCTGA AGCCAATTAT CCCCAACTTC GTTGCTCAGG 
     3361 GTGCAGAGAG GGTTAGCGAG GCAAATCCCA GTTTCAGGAA GAGCTTTGAA TTCAGTACGC 
     3421 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     3481 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     3541 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     3601 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     3661 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     3721 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     3781 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     3841 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     3901 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     3961 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4021 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4081 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4141 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4201 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4261 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4321 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4381 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     4441 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     4501 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     4561 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     4621 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     4681 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     4741 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     4801 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     4861 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     4921 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     4981 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5041 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5101 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5161 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5221 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     5281 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     5341 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     5401 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     5461 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     5521 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     5581 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     5641 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     5701 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     5761 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     5821 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     5881 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     5941 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6001 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6061 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6121 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6181 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6241 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     6301 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     6361 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     6421 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     6481 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     6541 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     6601 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6661 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     6721 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     6781 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     6841 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     6901 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     6961 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7021 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7081 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7141 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7201 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7261 GCGAATTTT
//