LOCUS       RC211163                9393 bp    DNA     circular  SYN 
DEFINITION  PTPRS (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, S (PTPRS), transcript variant 3
ACCESSION   NM_130853
VERSION     NM_130853.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5548..5639 
                     /label="Myc-DDK"  
     misc_feature    5669..6254 
                     /label="PolyA signal"  
     misc_feature    5726..5745 
                     /label="Restriction Enzyme"  
     misc_feature    6530..7202 
                     /label="ColE1"  
     misc_feature    7632..8426 
                     /label="Kan/Neo"  
     misc_feature    8461..8763 
                     /label="SV40 ori"  
     misc_feature    8849..9326 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5523..5530 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5523 
                     /label="PTPRS"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCCCACC TGGGGCCCTG GCATGGTGTC TGTGGTTGGT CCCATGGGCC 
     1081 TCCTTGTGGT CCTGCTCGTT GGAGGCTGTG CAGCAGAAGA GCCCCCCAGG TTTATCAAAG 
     1141 AACCCAAGGA CCAGATCGGC GTGTCGGGGG GTGTGGCCTC TTTCGTGTGT CAGGCCACGG 
     1201 GTGACCCCAA GCCACGAGTG ACCTGGAACA AGAAGGGCAA GAAGGTCAAC TCTCAGCGCT 
     1261 TTGAGACGAT TGAGTTTGAT GAGAGTGCAG GGGCAGTGCT GAGGATCCAG CCGCTGAGGA 
     1321 CACCGCGGGA TGAAAACGTG TACGAGTGTG TGGCCCAGAA CTCGGTTGGG GAGATCACAG 
     1381 TCCATGCCAA GCTTACTGTC CTCCGAGAGG ACCAGCTGCC CTCTGGCTTC CCCAACATCG 
     1441 ACATGGGCCC ACAGTTGAAG GTGGTGGAGC GGACACGGAC AGCCACCATG CTCTGTGCAG 
     1501 CCAGCGGCAA CCCTGACCCT GAGATCACCT GGTTCAAGGA CTTCCTGCCT GTGGATCCTA 
     1561 GTGCCAGCAA TGGACGCATC AAACAGCTGC GATCAGGAGC CCTGCAGATT GAAAGCAGTG 
     1621 AGGAAACCGA CCAGGGCAAA TATGAGTGTG TGGCCACCAA CAGCGCCGGC GTGCGCTACT 
     1681 CCTCACCTGC CAACCTCTAC GTGCGAGTCC GCCGCGTGGC CCCGCGCTTC TCCATCCTGC 
     1741 CCATGAGCCA CGAGATCATG CCAGGGGGCA ACGTGAACAT CACCTGCGTG GCCGTGGGCT 
     1801 CGCCCATGCC ATACGTGAAG TGGATGCAGG GGGCCGAGGA CCTGACCCCC GAGGATGACA 
     1861 TGCCCGTGGG TCGGAACGTG CTGGAACTCA CAGATGTCAA GGACTCGGCC AACTACACCT 
     1921 GCGTGGCCAT GTCCAGCCTG GGCGTCATTG AGGCGGTTGC TCAGATCACG GTGAAATCTC 
     1981 TCCCCAAAGC TCCCGGGACT CCCATGGTGA CTGAGAACAC AGCCACCAGC ATCACCATCA 
     2041 CGTGGGACTC GGGCAACCCA GATCCTGTGT CCTATTACGT CATCGAATAT AAATCCAAGA 
     2101 GCCAAGACGG GCCGTATCAG ATTAAAGAGG ACATCACCAC CACACGTTAC AGCATCGGCG 
     2161 GCCTGAGCCC CAACTCGGAG TACGAGATCT GGGTGTCGGC CGTCAACTCC ATCGGCCAGG 
     2221 GGCCCCCCAG CGAGTCCGTG GTCACCCGCA CAGGCGAGCA GGCCCCGGCC AGCGCGCCGC 
     2281 GGAACGTGCA AGCCCGGATG CTCAGCGCGA CCACCATGAT TGTGCAGTGG GAGGAGCCGG 
     2341 TGGAGCCCAA CGGCCTGATC CGCGGCTACC GCGTCTACTA CACCATGGAA CCGGAGCACC 
     2401 CCGTGGGCAA CTGGCAGAAG CACAACGTGG ACGACAGCCT GCTGACCACC GTGGGCAGCC 
     2461 TGCTGGAGGA CGAGACCTAC ACCGTGCGGG TGCTCGCCTT CACCTCCGTC GGCGACGGGC 
     2521 CCCTCTCGGA CCCCATCCAG GTCAAGACGC AGCAGGGAGT GCCGGGCCAG CCCATGAACC 
     2581 TGCGGGCCGA GGCCAGGTCG GAGACCAGCA TCACGCTGTC CTGGAGCCCC CCGCGGCAGG 
     2641 AGAGTATCAT CAAGTACGAG CTCCTCTTCC GGGAAGGCGA CCATGGCCGG GAGGTGGGAA 
     2701 GGACCTTCGA CCCGACGACT TCCTACGTGG TGGAGGACCT GAAGCCCAAC ACGGAGTACG 
     2761 CCTTCCGCCT GGCGGCCCGC TCGCCGCAGG GCCTGGGCGC CTTCACCCCC GTGGTGCGGC 
     2821 AGCGCACGCT GCAGTCCATC TCGCCCAAGA ACTTCAAGGT GAAAATGATC ATGAAGACAT 
     2881 CAGTTCTGCT CAGCTGGGAG TTCCCTGACA ACTACAACTC ACCCACACCC TACAAGATCC 
     2941 AGTACAATGG GCTCACACTG GATGTGGATG GCCGTACCAC CAAGAAGCTC ATCACGCACC 
     3001 TCAAGCCCCA CACCTTCTAC AACTTTGTGC TGACCAATCG CGGCAGCAGC CTGGGCGGCC 
     3061 TCCAGCAGAC GGTCACCGCC TGGACTGCCT TCAACCTGCT CAACGGCAAG CCCAGCGTCG 
     3121 CCCCCAAGCC TGATGCTGAC GGCTTCATCA TGGTGTATCT TCCTGACGGC CAGAGCCCCG 
     3181 TGCCTGTCCA GAGCTATTTC ATTGTGATGG TGCCACTGCG CAAGTCTCGT GGAGGCCAAT 
     3241 TCCTGACCCC GCTGGGTAGC CCAGAGGACA TGGATCTGGA AGAGCTCATC CAGGACATCT 
     3301 CACGGCTACA GAGGCGCAGC CTGCGGCACT CGCGTCAGCT GGAGGTGCCC CGGCCCTATA 
     3361 TTGCAGCTCG CTTCTCTGTG CTGCCACCCA CGTTCCATCC CGGCGACCAG AAGCAGTATG 
     3421 GCGGCTTCGA TAACCGGGGC CTGGAGCCCG GCCACCGCTA TGTCCTCTTC GTGCTTGCCG 
     3481 TGCTTCAGAA GAGCGAGCCT ACCTTTGCAG CCAGTCCCTT CTCAGACCCC TTCCAGCTGG 
     3541 ATAACCCGGA CCCCCAGCCC ATCGTGGATG GCGAGGAGGG GCTTATCTGG GTGATCGGGC 
     3601 CTGTGCTGGC CGTGGTCTTC ATAATCTGCA TTGTCATTGC TATCCTGCTC TACAAGAACA 
     3661 AACCCGACAG TAAACGCAAG GACTCAGAAC CCCGCACCAA ATGCCTCCTG AACAATGCCG 
     3721 ACCTCGCCCC TCACCACCCC AAGGACCCTG TGGAAATGAG ACGCATTAAC TTCCAGACTC 
     3781 CAGGCATGCT TAGCCACCCG CCAATTCCCA TCGCAGACAT GGCGGAGCAC ACGGAGCGGC 
     3841 TCAAGGCCAA CGACAGCCTC AAGCTCTCCC AGGAGTATGA GTCCATCGAC CCTGGACAGC 
     3901 AGTTCACATG GGAACATTCC AACCTGGAAG TGAACAAGCC GAAGAACCGC TATGCCAACG 
     3961 TCATCGCCTA TGACCACTCC CGTGTCATCC TCCAGCCCAT TGAAGGCATC ATGGGCAGTG 
     4021 ATTACATCAA TGCCAACTAC GTGGACGGCT ACCGGCGTCA GAACGCGTAC ATTGCCACGC 
     4081 AGGGGCCGCT GCCTGAGACC TTTGGGGACT TCTGGCGTAT GGTGTGGGAG CAGCGGTCGG 
     4141 CGACCATCGT CATGATGACG CGGCTGGAGG AGAAGTCACG GATCAAGTGT GATCAGTATT 
     4201 GGCCCAACAG AGGCACGGAG ACCTACGGCT TCATCCAGGT CACGTTGCTA GATACCATCG 
     4261 AGCTGGCCAC ATTCTGCGTC AGGACATTCT CTCTGCACAA GAATGGCTCC AGTGAGAAAC 
     4321 GCGAGGTCCG CCAGTTCCAG TTTACGGCGT GGCCGGACCA TGGCGTGCCC GAATACCCAA 
     4381 CGCCCTTCCT GGCTTTCCTG CGGAGAGTCA AGACCTGCAA CCCGCCAGAT GCCGGCCCCA 
     4441 TCGTGGTTCA CTGCAGTGCC GGTGTGGGCC GCACAGGCTG CTTTATCGTC ATCGACGCCA 
     4501 TGCTTGAGCG GATCAAGCCA GAGAAGACAG TCGATGTCTA TGGCCACGTG ACGCTCATGA 
     4561 GGTCCCAGCG CAACTACATG GTGCAGACGG AGGACCAGTA CAGCTTCATC CACGAGGCCC 
     4621 TGCTGGAGGC CGTGGGCTGT GGCAACACAG AAGTGCCCGC ACGCAGCCTC TATGCCTACA 
     4681 TCCAGAAGCT GGCCCAGGTG GAGCCTGGCG AACACGTCAC TGGCATGGAA CTCGAGTTCA 
     4741 AGCGGCTGGC TAACTCCAAG GCCCACACGT CACGCTTCAT CAGTGCCAAT CTGCCTTGTA 
     4801 ACAAGTTCAA GAACCGCCTG GTGAACATCA TGCCCTATGA GAGCACACGG GTCTGTCTGC 
     4861 AACCCATCCG GGGTGTGGAG GGCTCTGACT ACATCAACGC CAGCTTCATT GATGGCTACA 
     4921 GGCAGCAGAA GGCCTACATC GCGACACAGG GGCCGCTGGC GGAGACCACG GAAGACTTCT 
     4981 GGCGCATGCT GTGGGAGAAC AATTCGACGA TCGTGGTGAT GCTGACCAAG CTGCGGGAGA 
     5041 TGGGCCGGGA GAAGTGTCAC CAGTACTGGC CGGCCGAGCG CTCTGCCCGC TACCAGTACT 
     5101 TTGTGGTAGA TCCGATGGCA GAATACAACA TGCCTCAGTA TATCCTGCGA GAGTTCAAGG 
     5161 TCACAGATGC CCGGGATGGC CAGTCCCGGA CTGTCCGGCA GTTCCAGTTC ACAGACTGGC 
     5221 CGGAACAGGG TGTGCCAAAG TCGGGGGAGG GCTTCATCGA CTTCATTGGC CAAGTGCATA 
     5281 AGACTAAGGA GCAGTTTGGC CAGGACGGCC CCATCTCTGT CCACTGCAGT GCCGGCGTGG 
     5341 GCAGGACGGG CGTCTTCATC ACGCTTAGCA TCGTGCTGGA GCGGATGCGG TATGAAGGCG 
     5401 TGGTGGACAT CTTTCAGACG GTGAAGATGC TACGAACCCA GCGGCCGGCC ATGGTGCAGA 
     5461 CAGAGGATGA GTACCAGTTC TGTTACCAGG CGGCACTGGA GTACCTCGGA AGCTTTGACC 
     5521 ACTATGCAAC CAGCGGACCG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     5581 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5641 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5701 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5761 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5821 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5881 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5941 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     6001 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     6061 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     6121 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     6181 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     6241 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     6301 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     6361 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     6421 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     6481 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     6541 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6601 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6661 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6721 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6781 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6841 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6901 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6961 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     7021 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     7081 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     7141 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     7201 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     7261 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     7321 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     7381 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     7441 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     7501 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     7561 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7621 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7681 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7741 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7801 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7861 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7921 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7981 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     8041 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     8101 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     8161 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     8221 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     8281 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     8341 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     8401 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     8461 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     8521 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     8581 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8641 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8701 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8761 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8821 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8881 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8941 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     9001 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     9061 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     9121 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     9181 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     9241 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     9301 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     9361 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//