LOCUS       RC211142                8079 bp    DNA     circular  SYN 
DEFINITION  RPS6KC1 (Myc-DDK-tagged)-Human ribosomal protein S6 kinase, 52kDa, polypeptide 1 (RPS6KC1), transcript variant 1
ACCESSION   NM_012424
VERSION     NM_012424.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4236..4327 
                     /label="Myc-DDK"  
     misc_feature    4357..4942 
                     /label="PolyA signal"  
     misc_feature    4414..4433 
                     /label="Restriction Enzyme"  
     misc_feature    5218..5890 
                     /label="ColE1"  
     misc_feature    6320..7114 
                     /label="Kan/Neo"  
     misc_feature    7149..7451 
                     /label="SV40 ori"  
     misc_feature    7537..8014 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4218..4224 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4218 
                     /label="RPS6KC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCTCTTAC CGGGAGCGGA GTGCCGACCT GGCCCGTTTC TACACTGTCA 
     1081 CCGAGCCCCA GCGACACCCG AGGGGCTACA CAGTATATAA GGTCACCGCC CGGGTTGTTT 
     1141 CACGAAGAAA TCCAGAGGAT GTCCAGGAGA TAATTGTATG GAAGAGATAC AGTGATTTTA 
     1201 AGAAACTACA CAAAGAACTA TGGCAAATTC ACAAAAACTT ATTCCGACAT TCAGAGTTGT 
     1261 TTCCTCCATT TGCTAAAGGA ATAGTGTTTG GGCGATTTGA TGAAACTGTT ATCGAAGAGA 
     1321 GAAGACAATG TGCTGAAGAC CTGCTACAGT TCTCTGCCAA TATTCCTGCT CTTTACAATA 
     1381 GTAAACAGCT TGAAGACTTT TTCAAGGGTG GAATAATTAA TGATAGTTCT GAATTAATTG 
     1441 GTCCTGCTGA AGCTCACTCA GATTCCCTCA TTGATACCTT TCCTGAGTGT AGTACGGAAG 
     1501 GCTTCTCCAG TGACAGTGAT CTGGTATCTC TTACTGTTGA TGTGGATTCT CTTGCTGAGT 
     1561 TAGATGATGG AATGGCTTCC AATCAAAATT CTCCCATTAG AACTTTTGGT CTCAATCTTT 
     1621 CTTCGGATTC TTCAGCACTA GGGGCTGTTG CTTCTGACAG TGAACAGAGC AAAACAGAAG 
     1681 AAGAACGGGA AAGTCGTAGC CTCTTTCCTG GCAGTTTAAA GCCGAAGCTT GGCAAGAGAG 
     1741 ATTATTTGGA GAAAGCAGGA GAATTAATAA AGCTGGCTTT AAAAAAGGAA GAAGAAGACG 
     1801 ACTATGAAGC TGCTTCTGAT TTTTATAGGA AGGGAGTTGA TTTACTCCTA GAAGGTGTTC 
     1861 AAGGAGAGTC AAGCCCTACC CGTCGAGAAG CTGTGAAGAG AAGAACAGCC GAGTACCTCA 
     1921 TGCGGGCAGA AAGTATCTCT AGTCTTTATG GGAAACCTCA GCTTGATGAT GTATCTCAGC 
     1981 CTCCAGGATC ACTAAGTTCA AGGCCCCTTT GGAACCTAAG GAGCCCTGCC GAGGAGCTGA 
     2041 AGGCCTTCAG AGTCCTTGGG GTGATTGACA AGGTTTTACT TGTAATGGAC ACAAGGACAG 
     2101 AACAGACTTT CATTTTAAAA GGTCTAAGGA AAAGCAGTGA ATACAGCAGG AACAGAAAGA 
     2161 CCATCATCCC CCGCTGTGTG CCCAACATGG TGTGTCTGCA TAAGTACATC ATCTCTGAGG 
     2221 AGTCAGTATT TCTTGTGCTG CAGCATGCGG AAGGTGGCAA ACTGTGGTCA TATATCAGTA 
     2281 AATTTCTAAA CAGAAGTCCT GAAGAAAGCT TTGACATCAA GGAAGTGAAA AAACCTACAC 
     2341 TTGCAAAAGT TCACCTGCAG CAGCCAACTT CTAGTCCTCA GGACAGCAGT AGCTTTGAAT 
     2401 CCAGAGGAAG TGATGGTGGA AGCATGCTTA AAGCTCTGCC TTTGAAGAGT AGTCTTACTC 
     2461 CAAGTTCTCA AGATGACAGC AACCAGGAAG ATGATGGCCA AGATAGCTCT CCAAAGTGGC 
     2521 CAGATTCTGG TTCAAGTTCA GAAGAAGAAT GTACTACTAG TTATTTAACA TTATGCAATG 
     2581 AATATGGGCA AGAAAAGATT GAACCAGGGT CTTTGAATGA GGAGCCCTTC ATGAAGACTG 
     2641 AAGGGAATGG TGTTGATACA AAAGCTATTA AAAGCTTCCC AGCACACCTT GCTGCTGACA 
     2701 GTGACAGCCC CAGCACACAG CTGAGAGCTC ACGAGCTGAA GTTCTTCCCC AACGATGACC 
     2761 CAGAAGCAGT TAGTTCTCCA AGAACATCAG ATTCCCTCAG TAGATCAAAA AATAGCCCCA 
     2821 TGGAATTCTT TAGGATAGAC AGTAAGGATA GCGCAAGTGA ACTCCTGGGA CTTGACTTTG 
     2881 GAGAAAAATT GTATAGTCTA AAATCAGAAC CTTTGAAACC ATTCTTTACT CTTCCAGATG 
     2941 GAGACAGTGC TTCTAGGAGT TTTAATACTA GTGAAAGCAA GGTAGAGTTT AAAGCTCAGG 
     3001 ACACCATTAG CAGGGGCTCA GATGACTCAG TGCCAGTTAT TTCATTTAAA GATGCTGCTT 
     3061 TTGATGATGT CAGTGGTACT GATGAAGGAA GACCTGATCT TCTTGTAAAT TTACCTGGTG 
     3121 AATTGGAGTC AACAAGAGAA GCTGCAGCAA TGGGACCTAC TAAGTTTACA CAAACTAATA 
     3181 TAGGGATAAT AGAAAATAAA CTCTTGGAAG CCCCTGATGT TTTATGCCTC AGGCTTAGTA 
     3241 CTGAACAATG CCAAGCACAT GAGGAGAAAG GCATAGAGGA ACTGAGTGAT CCCTCTGGGC 
     3301 CCAAATCCTA TAGTATAACA GAGAAACACT ATGCACAGGA GGATCCCAGG ATGTTATTTG 
     3361 TAGCAGCTGT TGATCATAGT AGTTCAGGAG ATATGTCTTT GTTACCCAGC TCAGATCCTA 
     3421 AGTTTCAAGG ACTTGGAGTG GTTGAGTCAG CAGTAACTGC AAACAACACA GAAGAAAGCT 
     3481 TATTCCGTAT TTGTAGTCCA CTCTCAGGTG CTAATGAATA TATTGCAAGC ACAGACACTT 
     3541 TAAAAACAGA AGAAGTATTG CTGTTTACAG ATCAGACTGA TGATTTGGCT AAAGAGGAAC 
     3601 CAACTTCTTT ATTCCAGAGA GACTCTGAGA CTAAGGGTGA AAGTGGTTTA GTGCTAGAAG 
     3661 GAGACAAGGA AATACATCAG ATTTTTGAGG ACCTTGATAA AAAATTAGCA CTAGCCTCCA 
     3721 GGTTTTACAT CCCAGAGGGC TGCATTCAAA GATGGGCAGC TGAAATGGTG GTAGCCCTTG 
     3781 ATGCTTTACA TAGAGAGGGA ATTGTGTGCC GCGATTTGAA CCCAAACAAC ATCTTATTGA 
     3841 ATGATAGAGG ACACATTCAG CTAACGTATT TTAGCAGGTG GAGTGAGGTT GAAGATTCCT 
     3901 GTGACAGCGA TGCCATAGAG AGAATGTACT GTGCCCCAGA GGTTGGAGCA ATCACTGAAG 
     3961 AAACTGAAGC CTGTGATTGG TGGAGTTTGG GTGCTGTCCT CTTTGAACTT CTCACTGGCA 
     4021 AGACTCTGGT TGAATGCCAT CCAGCAGGAA TAAATACTCA CACTACTTTG AACATGCCAG 
     4081 AATGTGTCTC TGAAGAGGCT CGCTCACTCA TTCAACAGCT CTTGCAGTTC AATCCTCTGG 
     4141 AACGACTTGG TGCTGGAGTT GCTGGTGTTG AAGATATCAA ATCTCATCCA TTTTTTACCC 
     4201 CTGTGGATTG GGCAGAACTG ATGAGAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4261 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4321 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4381 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4441 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4501 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4561 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4621 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4681 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4741 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4801 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4861 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4921 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4981 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5041 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5101 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5161 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5221 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5281 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5341 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5401 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5461 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5521 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5581 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5641 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5701 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5761 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5821 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5881 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5941 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6001 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6061 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6121 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6181 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6241 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6301 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6361 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6421 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6481 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6541 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6601 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6661 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6721 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6781 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6841 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6901 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6961 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7021 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7081 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7141 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7201 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7261 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7321 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7381 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7441 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7501 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7561 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7621 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7681 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7741 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7801 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7861 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7921 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7981 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8041 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//