LOCUS       RC211126                7503 bp    DNA     circular  SYN 
DEFINITION  TUT1 (Myc-DDK-tagged)-Human terminal uridylyl transferase 1, U6 snRNA-specific (TUT1)
ACCESSION   NM_022830
VERSION     NM_022830.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3660..3751 
                     /label="Myc-DDK"  
     misc_feature    3781..4366 
                     /label="PolyA signal"  
     misc_feature    3838..3857 
                     /label="Restriction Enzyme"  
     misc_feature    4642..5314 
                     /label="ColE1"  
     misc_feature    5744..6538 
                     /label="Kan/Neo"  
     misc_feature    6573..6875 
                     /label="SV40 ori"  
     misc_feature    6961..7438 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3642..3648 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3642 
                     /label="TUT1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGGTG GATTCGGATG TCGAATCGCT GCCGCGTGGG GGGTTCCGCT 
     1081 GCTGCCTCTG CCACGTTACT ACAGCCAACC GACCCAGCCT TGATGCCCAC TTGGGAGGCA 
     1141 GAAAGCACCG GCACCTGGTA GAACTACGAG CTGCGAGAAA GGCCCAGGGA CTTCGAAGTG 
     1201 TGTTTGTCAG TGGCTTTCCC AGGGATGTGG ATTCTGCTCA GCTCTCTGAG TACTTCCTAG 
     1261 CATTTGGACC TGTGGCCAGT GTTGTCATGG ACAAGGACAA GGGAGTGTTT GCCATTGTGG 
     1321 AGATGGGGGA CGTGGGTGCT CGAGAGGCTG TCTTGTCACA GTCCCAGCAC AGCCTGGGAG 
     1381 GACATCGCCT GCGTGTCCGC CCACGGGAGC AGAAGGAGTT CCAGAGCCCG GCCTCCAAAT 
     1441 CCCCCAAAGG AGCGGCCCCC GACAGTCACC AGCTGGCCAA AGCGCTAGCT GAGGCTGCAG 
     1501 ACGTGGGGGC ACAAATGATA AAGCTTGTGG GGCTGAGGGA GTTGTCCGAG GCCGAGCGGC 
     1561 AGCTTCGCAG CCTAGTGGTG GCCCTGATGC AGGAGGTCTT CACAGAGTTC TTCCCTGGCT 
     1621 GTGTGGTCCA CCCTTTTGGC TCTTCCATAA ATAGCTTCGA TGTCCATGGC TGTGATCTTG 
     1681 ACCTCTTCTT GGATCTGGGT GACTTGGAAG AGCCCCAGCC AGTCCCAAAG GCTCCAGAAT 
     1741 CTCCATCGCT GGACTCGGCC CTGGCTTCCC CACTGGACCC TCAAGCCCTG GCCTGCACCC 
     1801 CAGCTTCCCC TCCAGATTCA CAACCTCCTG CTTCTCCCCA GGATTCTGAA GCCCTGGACT 
     1861 TTGAAACCCC TTCCTCCTCC CTGGCGCCCC AAACTCCGGA CTCTGCCTTG GCCTCCGAGA 
     1921 CCCTTGCTTC TCCCCAGTCT CTGCCTCCAG CTTCACCACT GCTAGAGGAC AGGGAAGAGG 
     1981 GGGACCTGGG GAAGGCCTCG GAACTAGCAG AGACCCCAAA GGAGGAGAAA GCAGAGGGGG 
     2041 CAGCAATGCT GGAGCTGGTG GGATCCATTC TCCGGGGCTG TGTCCCTGGA GTGTATCGAG 
     2101 TCCAAACTGT GCCCTCTGCC CGGCGCCCTG TGGTCAAGTT CTGTCATCGG CCTTCAGGTC 
     2161 TCCACGGTGA TGTCTCCCTC AGTAACCGGC TGGCCCTGCA TAACTCCCGT TTCCTGAGTC 
     2221 TCTGCTCTGA GCTGGATGGT CGAGTCCGGC CCCTCGTGTA CACCCTCCGC TGCTGGGCTC 
     2281 AGGGTCGGGG GCTGTCAGGG AGTGGCCCCC TTCTCAGTAA CTACGCCCTG ACCTTGCTGG 
     2341 TGATCTATTT TCTTCAGACC AGGGACCCTC CTGTGTTGCC CACTGTGTCC CAGCTCACCC 
     2401 AGAAAGCAGG AGAGGGGGAA CAGGTGGAAG TCGATGGCTG GGACTGCAGT TTCCCCAGGG 
     2461 ATGCCTCAAG ACTGGAGCCC AGCATAAATG TGGAGCCCCT CAGTTCCCTG CTAGCCCAGT 
     2521 TCTTCTCCTG TGTATCTTGT TGGGATCTTC GTGGCTCCCT GCTGTCCCTG CGGGAGGGTC 
     2581 AGGCACTGCC TGTGGCAGGG GGCCTGCCTT CTAATCTCTG GGAGGGTCTG CGCCTTGGCC 
     2641 CCCTGAATCT CCAGGACCCT TTTGACCTGA GTCACAATGT CGCAGCCAAT GTGACCAGCC 
     2701 GGGTGGCTGG GCGCCTACAG AACTGCTGCC GAGCAGCAGC CAATTACTGC CGAAGCCTCC 
     2761 AGTACCAGCG CCGTTCCTCC CGGGGTCGGG ACTGGGGGCT GCTCCCTCTT CTGCAGCCCA 
     2821 GCTCCCCCAG CTCCCTGCTC TCTGCTACGC CGATCCCTTT ACCCCTTGCA CCCTTCACCC 
     2881 AGCTCACTGC TGCCCTGGTG CAGGTATTCA GGGAAGCACT GGGGTGCCAT ATAGAACAGG 
     2941 CAACCAAGAG AACGCGGTCA GAAGGAGGTG GAACTGGGGA GTCCTCTCAG GGAGGGACAA 
     3001 GCAAAAGACT CAAAGTAGAT GGACAGAAAA ACTGCTGTGA GGAGGGGAAA GAGGAGCAGC 
     3061 AGGGATGTGC AGGGGACGGT GGGGAAGACA GGGTAGAAGA GATGGTTATA GAGGTTGGAG 
     3121 AGATGGTGCA GGACTGGGCC ATGCAGAGCC CTGGGCAGCC AGGGGACCTG CCCCTGACCA 
     3181 CTGGAAAGCA TGGAGCCCCT GGAGAAGAGG GGCAGCCCAG CCACGCAGCC CTGGCAGAGC 
     3241 GGGGGCCCAA GGGACATGAG GCAGCCCAAG AATGGTCTCA GGGTGAGGCA GGGAAGGGGG 
     3301 CATCCCTGCC CTCCTCAGCG AGCTGGCGCT GTGCCTTGTG GCACCGAGTG TGGCAAGGGC 
     3361 GGCGGCGAGC CCGTAGACGC TTGCAGCAGC AAACCAAGGA GGGAGCTGGA GGTGGCGCTG 
     3421 GCACAAGAGC AGGGTGGCTG GCGACTGAGG CTCAGGTCAC CCAGGAGCTG AAAGGACTGA 
     3481 GTGGTGGCGA AGAGAGGCCA GAAACTGAGC CCCTGCTGAG CTTTGTGGCG TCTGTCTCCC 
     3541 CGGCTGACCG AATGCTCACT GTGACCCCGC TCCAGGATCC CCAAGGCCTG TTCCCTGATC 
     3601 TCCATCATTT CTTACAGGTT TTCCTCCCTC AAGCAATTCG ACATCTCAAG ACGCGTACGC 
     3661 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     3721 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     3781 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     3841 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     3901 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     3961 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4021 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4081 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4141 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4201 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4261 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4321 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4381 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4441 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4501 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4561 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4621 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     4681 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     4741 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     4801 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     4861 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     4921 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     4981 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5041 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5101 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5161 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5221 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5281 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5341 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5401 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5461 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5521 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5581 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     5641 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     5701 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     5761 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     5821 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     5881 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     5941 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6001 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6061 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6121 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6181 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6241 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6301 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6361 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6421 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6481 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6541 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6601 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     6661 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     6721 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     6781 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     6841 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     6901 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     6961 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7021 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7081 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7141 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7201 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7261 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7321 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7381 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7441 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7501 TTT
//