LOCUS       RC211111L1                9300 bp    DNA     circular  SYN 
DEFINITION  NLRX1 (Myc-DDK-tagged)-Human NLR family member X1 (NLRX1), transcript variant 1
ACCESSION   NM_024618
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7591..7771 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8499..9158 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7930..8498 
                     /label="ColE1"  
     misc_feature    6466..6486 
                     /label="LR50 primer"  
     misc_feature    6263..6334 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6475..7066 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6245..6251 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6245 
                     /label="NLRX1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAGGTGGGGC CACCATTTGC CCAGGGCCTC 
     3361 TTGGGGCTCT GGTTTTAGAA GAGCACTCCA GCGACCAGAT GATCGTATCC CCTTCCTGAT 
     3421 CCACTGGAGT TGGCCCCTTC AAGGGGAGCG TCCCTTTGGG CCCCCTAGGG CCTTTATACG 
     3481 CCACCACGGA AGCTCGGTAG ATAGCGCTCC CCCACCCGGG AGGCATGGAC GGCTGTTCCC 
     3541 CAGCGCCTCT GCAACTGAAG CTATACAGCG GCACCGCCGG AACCTGGCTG AGTGGTTCAG 
     3601 CCGGCTGCCC AGGGAGGAGC GCCAGTTTGG CCCAACCTTT GCCCTAGACA CGGTCCACGT 
     3661 TGACCCTGTG ATCCGCGAGA GTACCCCTGA TGAGCTACTT CGCCCACCCG CGGAGCTGGC 
     3721 CCTGGAGCAT CAGCCACCCC AGGCCGGGCT CCCCCCACTG GCCTTGTCTC AGCTCTTTAA 
     3781 CCCGGATGCC TGTGGGCGCC GGGTGCAGAC AGTGGTGCTG TATGGGACAG TGGGCACAGG 
     3841 CAAGAGCACG CTGGTGCGCA AGATGGTTCT GGACTGGTGT TATGGGCGGC TGCCGGCCTT 
     3901 CGAGCTGCTC ATCCCCTTCT CCTGTGAGGA CCTGTCATCC CTGGGCCCTG CCCCAGCCTC 
     3961 CCTGTGCCAA CTTGTGGCCC AGCGCTACAC GCCCCTGAAG GAGGTTCTGC CCCTGATGGC 
     4021 TGCTGCTGGG TCCCACCTCC TCTTTGTGCT CCATGGCTTA GAGCATCTCA ACCTCGACTT 
     4081 CCGGCTGGCA GGCACGGGAC TTTGTAGTGA CCCGGAGGAA CCGCAGGAAC CAGCTGCTAT 
     4141 CATCGTCAAC CTGCTGCGCA AATACATGCT GCCTCAGGCC AGCATTCTGG TGACCACTCG 
     4201 GCCCTCTGCC ATTGGCCGTA TCCCCAGCAA GTACGTGGGC CGCTATGGTG AGATCTGCGG 
     4261 TTTCTCTGAT ACCAACCTGC AGAAGCTCTA CTTCCAGCTC CGCCTCAACC AGCCGTACTG 
     4321 CGGGTATGCC GTTGGCGGTT CAGGTGTCTC TGCCACACCA GCTCAGCGTG ACCACCTGGT 
     4381 GCAGATGCTC TCCCGGAACC TGGAGGGGCA CCACCAGATA GCCGCTGCCT GCTTCCTGCC 
     4441 GTCCTATTGC TGGCTCGTTT GTGCCACCTT GCACTTCCTG CATGCCCCCA CGCCTGCTGG 
     4501 GCAGACCCTT ACAAGCATCT ATACCAGCTT CCTGCGCCTC AACTTCAGCG GGGAAACCCT 
     4561 GGACAGCACT GACCCCTCCA ATTTGTCCCT GATGGCCTAT GCAGCCCGAA CCATGGGCAA 
     4621 GTTGGCCTAT GAGGGGGTGT CCTCCCGCAA GACCTACTTC TCTGAAGAGG ATGTCTGTGG 
     4681 CTGCCTGGAG GCTGGCATCA GGACGGAGGA GGAGTTTCAG CTGCTGCACA TCTTCCGTCG 
     4741 GGATGCCCTG AGGTTTTTCC TGGCCCCATG TGTGGAGCCA GGGCGTGCAG GCACCTTCGT 
     4801 GTTCACCGTG CCCGCCATGC AGGAATACCT GGCTGCCCTC TACATTGTGC TGGGTTTGCG 
     4861 CAAGACGACC CTGCAAAAGG TGGGCAAGGA AGTGGCTGAG CTCGTGGGCC GTGTTGGGGA 
     4921 GGACGTCAGC CTGGTACTGG GCATCATGGC CAAGCTGCTG CCTCTGCGGG CTCTGCCTCT 
     4981 GCTCTTCAAC CTGATCAAGG TGGTTCCACG AGTGTTTGGG CGCATGGTGG GTAAAAGCCG 
     5041 GGAGGCGGTG GCTCAGGCCA TGGTGCTGGA GATGTTTCGA GAGGAGGACT ACTACAACGA 
     5101 TGATGTTCTG GACCAGATGG GCGCCAGTAT CCTGGGCGTG GAGGGCCCCC GGCGCCACCC 
     5161 AGATGAGCCC CCTGAGGATG AAGTCTTCGA GCTCTTCCCC ATGTTCATGG GGGGGCTTCT 
     5221 CTCTGCCCAC AACCGAGCTG TGCTAGCTCA GCTTGGCTGC CCCATCAAGA ACCTGGATGC 
     5281 CCTGGAGAAT GCCCAGGCCA TCAAGAAGAA GCTGGGCAAG CTGGGCCGGC AGGTGCTGCC 
     5341 CCCATCAGAG CTCCTTGACC ACCTCTTCTT CCACTATGAG TTCCAGAACC AGCGCTTCTC 
     5401 CGCTGAGGTG CTCAGCTCCC TGCGTCAGCT CAACCTGGCA GGTGTGCGCA TGACACCAGT 
     5461 CAAGTGCACA GTGGTGGCAG CTGTGCTGGG CAGCGGAAGG CATGCCCTGG ATGAGGTGAA 
     5521 CTTGGCCTCC TGCCAGCTAG ATCCTGCTGG GCTGCGCACA CTCCTGCCTG TCTTCCTGCG 
     5581 TGCCCGGAAG CTGGGCTTGC AACTCAACAG CCTGGGCCCT GAGGCCTGCA AGGACCTCCG 
     5641 AGACCTGTTG CTGCATGACC AGTGCCAAAT TACCACACTG CGGCTGTCCA ACAACCCGCT 
     5701 GACGGCGGCA GGTGTTGCCG TGCTAATGGA GGGGCTGGCA GGAAACACCT CAGTGACGCA 
     5761 CCTGTCCCTG CTGCACACGG GCCTTGGGGA CGAAGGCCTG GAGCTGCTGG CTGCCCAGCT 
     5821 GGACCGCAAC CGGCAGCTGC AGGAGCTGAA CGTGGCGTAC AACGGTGCTG GTGACACAGC 
     5881 GGCCCTGGCC CTGGCCAGAG CTGCCCGGGA GCACCCTTCC CTGGAACTGC TACACCTCTA 
     5941 CTTCAATGAG CTGAGCTCAG AGGGCCGCCA GGTCTTGCGA GACTTGGGGG GTGCTGCTGA 
     6001 AGGTGGTGCC CGGGTGGTGG TGTCACTGAC AGAGGGGACG GCGGTGTCAG AATACTGGTC 
     6061 AGTGATCCTC AGTGAAGTCC AGCGGAACCT CAATAGCTGG GATCGGGCCC GGGTTCAGCG 
     6121 ACACCTTGAG CTCCTACTGC GGGATCTGGA AGATAGCCGG GGTGCCACCC TTAATCCTTG 
     6181 GCGCAAGGCC CAGCTGCTGC GAGTGGAGGG CGAGGTCAGG GCCCTCCTGG AGCAGCTGGG 
     6241 AAGCTCTGGA AGCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT 
     6301 GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG 
     6361 CGGTCTGTAC AAGTAGGATT CGTCGAGGGA CCTAATAACT TCGTATAGCA TACATTATAC 
     6421 GAAGTTATAC ATGTTTAAGG GTTCCGGTTC CACTAGGTAC AATTCGATAT CAAGCTTATC 
     6481 GATAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT 
     6541 GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC 
     6601 CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG 
     6661 TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC 
     6721 ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC 
     6781 CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG 
     6841 CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG 
     6901 CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC 
     6961 CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT 
     7021 CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCATCGATAC 
     7081 CGTCGACCTC GATCGAGACC TAGAAAAACA TGGAGCAATC ACAAGTAGCA ATACAGCAGC 
     7141 TACCAATGCT GATTGTGCCT GGCTAGAAGC ACAAGAGGAG GAGGAGGTGG GTTTTCCAGT 
     7201 CACACCTCAG GTACCTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC TTAGCCACTT 
     7261 TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT AATTCACTCC CAACGAAGAC AAGATATCCT 
     7321 TGATCTGTGG ATCTACCACA CACAAGGCTA CTTCCCTGAT TGGCAGAACT ACACACCAGG 
     7381 GCCAGGGATC AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCA 
     7441 AGAGAAGGTA GAAGAAGCCA ATGAAGGAGA GAACACCCGC TTGTTACACC CTGTGAGCCT 
     7501 GCATGGGATG GATGACCCGG AGAGAGAAGT ATTAGAGTGG AGGTTTGACA GCCGCCTAGC 
     7561 ATTTCATCAC ATGGCCCGAG AGCTGCATCC GGACTGTACT GGGTCTCTCT GGTTAGACCA 
     7621 GATCTGAGCC TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG 
     7681 CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG 
     7741 ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGCATGTGAG CAAAAGGCCA 
     7801 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     7861 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     7921 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     7981 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     8041 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     8101 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     8161 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     8221 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     8281 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     8341 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     8401 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     8461 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGATTA CGCCCCGCCC 
     8521 TGCCACTCAT CGCAGTACTG TTGTAATTCA TTAAGCATTC TGCCGACATG GAAGCCATCA 
     8581 CAAACGGCAT GATGAACCTG AATCGCCAGC GGCATCAGCA CCTTGTCGCC TTGCGTATAA 
     8641 TATTTGCCCA TGGTGAAAAC GGGGGCGAAG AAGTTGTCCA TATTGGCCAC GTTTAAATCA 
     8701 AAACTGGTGA AACTCACCCA GGGATTGGCT GAGACGAAAA ACATATTCTC AATAAACCCT 
     8761 TTAGGGAAAT AGGCCAGGTT TTCACCGTAA CACGCCACAT CTTGCGAATA TATGTGTAGA 
     8821 AACTGCCGGA AATCGTCGTG GTATTCACTC CAGAGCGATG AAAACGTTTC AGTTTGCTCA 
     8881 TGGAAAACGG TGTAACAAGG GTGAACACTA TCCCATATCA CCAGCTCACC GTCTTTCATT 
     8941 GCCATACGGA ACTCCGGATG AGCATTCATC AGGCGGGCAA GAATGTGAAT AAAGGCCGGA 
     9001 TAAAACTTGT GCTTATTTTT CTTTACGGTC TTTAAAAAGG CCGTAATATC CAGCTGAACG 
     9061 GTCTGGTTAT AGGTACATTG AGCAACTGAC TGAAATGCCT CAAAATGTTC TTTACGATGC 
     9121 CATTGGGATA TATCAACGGT GGTATATCCA GTGATTTTTT TCTCCATACT CTTCCTTTTT 
     9181 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     9241 ATTTAGAAAA ATAAACAAAT AGGGGTCCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
//