LOCUS       RC211091                8277 bp    DNA     circular  SYN 
DEFINITION  INPP5F (Myc-DDK-tagged)-Human inositol polyphosphate-5-phosphatase F (INPP5F), transcript variant 1
ACCESSION   NM_014937
VERSION     NM_014937.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4434..4525 
                     /label="Myc-DDK"  
     misc_feature    4555..5140 
                     /label="PolyA signal"  
     misc_feature    4612..4631 
                     /label="Restriction Enzyme"  
     misc_feature    5416..6088 
                     /label="ColE1"  
     misc_feature    6518..7312 
                     /label="Kan/Neo"  
     misc_feature    7347..7649 
                     /label="SV40 ori"  
     misc_feature    7735..8212 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4416..4422 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4416 
                     /label="INPP5F"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCTCTTC CAAGCCAAGG ACCACTACAT CCTGCAGCAG GGCGAGCGCG 
     1081 CGCTGTGGTG CAGCCGCCGC GACGGCGGCC TCCAGCTCCG ACCCGCTACT GATCTACTTC 
     1141 TTGCCTGGAA TCCCATTTGT TTGGGGTTGG TAGAAGGTGT TATTGGGAAA ATTCAACTTC 
     1201 ATTCAGATCT TCCATGGTGG CTTATTCTAA TTCGGCAGAA AGCATTGGTG GGCAAACTCC 
     1261 CAGGAGACCA TGAGGTCTGT AAAGTTACCA AAATTGCTGT GCTCTCACTT TCTGAAATGG 
     1321 AACCTCAGGA TCTTGAGCTA GAGCTCTGTA AGAAGCATCA TTTTGGTATT AACAAACCAG 
     1381 AGAAGATCAT ACCATCTCCT GATGACTCAA AGTTTCTACT GAAGACCTTT ACGCATATTA 
     1441 AATCCAATGT GTCTGCTCCT AATAAAAAGA AAGTTAAGGA AAGTAAAGAG AAGGAGAAGT 
     1501 TGGAGAGGAG ATTACTTGAA GAGTTGCTGA AGATGTTCAT GGACTCAGAA TCCTTTTATT 
     1561 ATAGCTTGAC CTATGACCTG ACCAATTCCG TGCAGAGGCA GAGCACTGGG GAGAGGGACG 
     1621 GTCGGCCCCT CTGGCAGAAG GTTGATGACC GATTTTTTTG GAATAAATAC ATGATACAAG 
     1681 ATCTTACTGA GATTGGTACT CCAGATGTGG ACTTTTGGAT TATCCCCATG ATCCAAGGTT 
     1741 TTGTGCAGAT TGAAGAACTT GTGGTTAATT ATACCGAATC ATCTGATGAT GAGAAAAGCA 
     1801 GCCCAGAGAC CCCCCCTCAG GAGTCCACCT GTGTAGATGA TATTCACCCA CGATTTCTAG 
     1861 TGGCTCTCAT TTCACGCCGA AGTAGGCACA GAGCAGGAAT GCGCTATAAA CGAAGAGGAG 
     1921 TGGATAAAAA TGGAAATGTT GCCAATTATG TGGAGACTGA GCAGTTGATT CATGTTCATA 
     1981 ATCATACCCT GTCATTTGTT CAAACACGAG GCTCTGTGCC TGTCTTTTGG AGCCAGGTTG 
     2041 GGTATCGATA TAACCCAAGA CCGCGGCTGG ACAGAAGTGA AAAGGAAACT GTTGCCTATT 
     2101 TCTGTGCCCA TTTCGAAGAA CAACTGAACA TTTACAAAAA ACAGGTTATT ATTAACTTGG 
     2161 TAGACCAGGC AGGAAGAGAG AAGGTTATTG GCGATGCTTA CCTGAAGCAA GTGTTGCTTT 
     2221 TCAACAACTC ACACCTCACT TACGTTTCGT TTGACTTCCA TGAGCACTGC CGAGGAATGA 
     2281 AGTTTGAGAA TGTTCAGACA CTAACAGATG CCATTTATGA CATTATTCTT GATATGAAGT 
     2341 GGTGTTGGGT TGATGAAGCT GGGGTAATAT GTAAGCAGGA AGGGATTTTT CGTGTTAATT 
     2401 GTATGGACTG CCTGGATCGC ACCAACGTGG TCCAAGCTGC CATCGCGAGA GTGGTCATGG 
     2461 AACAGCAGCT GAAAAAATTA GGTGTGATGC CCCCGGAACA GCCATTACCT GTGAAATGTA 
     2521 ATCGCATCTA CCAGATAATG TGGGCCAATA ATGGTGACTC CATTAGCAGA CAGTATGCTG 
     2581 GGACAGCTGC TCTGAAGGGT GACTTTACAA GGACAGGAGA AAGGAAGTTA GCAGGAGTTA 
     2641 TGAAAGATGG AGTGAACTCA GCAAACAGAT ATTACCTCAA CCGATTTAAG GATGCTTATA 
     2701 GGCAAGCTGT TATAGATTTG ATGCAAGGCA TTCCAGTGAC AGAAGATCTT TATTCCATAT 
     2761 TTACCAAGGA GAAAGAACAT GAAGCTTTGC ATAAGGAAAA TCAGAGAAGC CACCAGGAAC 
     2821 TAATTAGCCA GCTCTTACAA AGTTACATGA AGTTACTACT GCCTGATGAT GAGAAGTTCC 
     2881 ATGGGGGCTG GGCCCTCATT GACTGTGACC CTAGCCTCAT TGATGCTACT CACAGAGACG 
     2941 TGGATGTGCT GTTACTGCTT TCTAACTCTG CCTACTACGT GGCCTATTAT GATGATGAAG 
     3001 TTGATAAAGT AAACCAGTAT CAACGACTAA GTCTAGAAAA CCTGGAAAAA ATTGAAATAG 
     3061 GCCCTGAACC CACTCTTTTT GGTAAGCCAA AGTTCTCCTG CATGCGACTG CACTACAGAT 
     3121 ACAAAGAAGC GAGTGGCTAT TTCCACACAT TGCGAGCTGT AATGCGTAAT CCTGAAGAGG 
     3181 ATGGAAAAGA TACCCTTCAG TGCATTGCAG AGATGCTGCA GATCACCAAG CAAGCCATGG 
     3241 GATCGGATTT ACCCATAATT GAGAAGAAAC TTGAGAGGAA GAGCAGTAAA CCTCACGAAG 
     3301 ACATCATTGG TATCAGGTCT CAAAACCAAG GTTCTTTGGC CCAGGGAAAG AATTTTTTAA 
     3361 TGAGCAAATT TTCATCTCTA AATCAAAAAG TGAAGCAGAC CAAATCCAAT GTAAATATTG 
     3421 GCAACCTCCG AAAGCTAGGA AACTTTACCA AACCTGAAAT GAAAGTTAAC TTTCTAAAAC 
     3481 CAAACTTAAA AGTAAATCTT TGGAAATCAG ATAGTAGTCT TGAAACTATG GAAAACACAG 
     3541 GAGTGATGGA TAAGGTTCAG GCAGAGTCTG ATGGGGACAT GTCTTCAGAT AATGACTCAT 
     3601 ACCACTCTGA TGAATTCCTT ACAAATTCTA AGTCTGATGA AGACAGGCAG CTAGCTAACT 
     3661 CATTAGAGAG TGTAGGGCCA ATAGATTACG TTCTTCCTAG TTGTGGTATT ATTGCCTCAG 
     3721 CGCCTCGATT GGGCAGTCGG TCCCAGTCTC TTAGCAGCAC AGATAGTAGC GTTCATGCTC 
     3781 CTTCAGAGAT TACTGTTGCT CATGGGAGTG GGCTTGGAAA AGGCCAGGAG TCTCCTTTGA 
     3841 AGAAAAGTCC TTCTGCTGGC GACGTACACA TATTGACTGG CTTTGCCAAG CCTATGGATA 
     3901 TTTACTGCCA CAGATTTGTG CAAGATGCAC AGAACAAAGT GACCCACCTA TCAGAGACCA 
     3961 GATCTGTGTC TCAGCAGGCT AGTCAGGAAA GAAATCAAAT GACCAATCAA GTTTCAGATG 
     4021 AAACCCAATC AGAATCAACA GAACAGACAC CTTCTCGGCC ATCGCAATTA GATGTCTCTC 
     4081 TTTCTGCAAC AGGCCCACAG TTTTTGTCAG TTGAGCCAGC GCATTCAGTT GCATCTCAAA 
     4141 AAACCCCCAC CTCCGCTTCC AGCATGCTTG AACTTGAGAC AGGGCTTCAT GTAACTCCTT 
     4201 CTCCTTCAGA GAGCAGTAGC AGCAGAGCAG TCTCTCCCTT TGCCAAGATT CGAAGTTCCA 
     4261 TGGTCCAGGT TGCTAGTATT ACCCAAGCTG GATTAACCCA TGGGATAAAC TTTGCAGTGT 
     4321 CAAAAGTTCA GAAGAGTCCT CCAGAACCTG AAATCATTAA TCAAGTCCAG CAAAATGAAC 
     4381 TTAAAAAGAT GTTTATACAA TGCCAGACAC GGATAATTCA GATTACGCGT ACGCGGCCGC 
     4441 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4501 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4561 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4621 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4681 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4741 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4801 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4861 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4921 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4981 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5041 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5101 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5161 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5221 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5281 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5341 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5401 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5461 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5521 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5581 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5641 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5701 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5761 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5821 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5881 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5941 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6001 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6061 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6121 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6181 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6241 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6301 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6361 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6421 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6481 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6541 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6601 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6661 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6721 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6781 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6841 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6901 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6961 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7021 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7081 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7141 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7201 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7261 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7321 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7381 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7441 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7501 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7561 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7621 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7681 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7741 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7801 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7861 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7921 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7981 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8041 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8101 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8161 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8221 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//