LOCUS       RC211013L2                9333 bp    DNA     circular  SYN 
DEFINITION  BRAF (Myc-DDK-tagged)-Human v-raf murine sarcoma viral oncogene homolog B1 (BRAF)
ACCESSION   NM_004333
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7624..7804 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8532..9191 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7852..8471 
                     /label="ColE1"  
     misc_feature    6499..6519 
                     /label="LR50"  
     misc_feature    5639..6364 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    6508..7099 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5618..5624 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5618 
                     /label="BRAF"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCGGCGCTG AGCGGTGGCG GTGGTGGCGG 
     3361 CGCGGAGCCG GGCCAGGCTC TGTTCAACGG GGACATGGAG CCCGAGGCCG GCGCCGGCGC 
     3421 CGGCGCCGCG GCCTCTTCGG CTGCGGACCC TGCCATTCCG GAGGAGGTGT GGAATATCAA 
     3481 ACAAATGATT AAGTTGACAC AGGAACATAT AGAGGCCCTA TTGGACAAAT TTGGTGGGGA 
     3541 GCATAATCCA CCATCAATAT ATCTGGAGGC CTATGAAGAA TACACCAGCA AGCTAGATGC 
     3601 ACTCCAACAA AGAGAACAAC AGTTATTGGA ATCTCTGGGG AACGGAACTG ATTTTTCTGT 
     3661 TTCTAGCTCT GCATCAATGG ATACCGTTAC ATCTTCTTCC TCTTCTAGCC TTTCAGTGCT 
     3721 ACCTTCATCT CTTTCAGTTT TTCAAAATCC CACAGATGTG GCACGGAGCA ACCCCAAGTC 
     3781 ACCACAAAAA CCTATCGTTA GAGTCTTCCT GCCCAACAAA CAGAGGACAG TGGTACCTGC 
     3841 AAGGTGTGGA GTTACAGTCC GAGACAGTCT AAAGAAAGCA CTGATGATGA GAGGTCTAAT 
     3901 CCCAGAGTGC TGTGCTGTTT ACAGAATTCA GGATGGAGAG AAGAAACCAA TTGGTTGGGA 
     3961 CACTGATATT TCCTGGCTTA CTGGAGAAGA ATTGCATGTG GAAGTGTTGG AGAATGTTCC 
     4021 ACTTACAACA CACAACTTTG TACGAAAAAC GTTTTTCACC TTAGCATTTT GTGACTTTTG 
     4081 TCGAAAGCTG CTTTTCCAGG GTTTCCGCTG TCAAACATGT GGTTATAAAT TTCACCAGCG 
     4141 TTGTAGTACA GAAGTTCCAC TGATGTGTGT TAATTATGAC CAACTTGATT TGCTGTTTGT 
     4201 CTCCAAGTTC TTTGAACACC ACCCAATACC ACAGGAAGAG GCGTCCTTAG CAGAGACTGC 
     4261 CCTAACATCT GGATCATCCC CTTCCGCACC CGCCTCGGAC TCTATTGGGC CCCAAATTCT 
     4321 CACCAGTCCG TCTCCTTCAA AATCCATTCC AATTCCACAG CCCTTCCGAC CAGCAGATGA 
     4381 AGATCATCGA AATCAATTTG GGCAACGAGA CCGATCCTCA TCAGCTCCCA ATGTGCATAT 
     4441 AAACACAATA GAACCTGTCA ATATTGATGA CTTGATTAGA GACCAAGGAT TTCGTGGTGA 
     4501 TGGAGGATCA ACCACAGGTT TGTCTGCTAC CCCCCCTGCC TCATTACCTG GCTCACTAAC 
     4561 TAACGTGAAA GCCTTACAGA AATCTCCAGG ACCTCAGCGA GAAAGGAAGT CATCTTCATC 
     4621 CTCAGAAGAC AGGAATCGAA TGAAAACACT TGGTAGACGG GACTCGAGTG ATGATTGGGA 
     4681 GATTCCTGAT GGGCAGATTA CAGTGGGACA AAGAATTGGA TCTGGATCAT TTGGAACAGT 
     4741 CTACAAGGGA AAGTGGCATG GTGATGTGGC AGTGAAAATG TTGAATGTGA CAGCACCTAC 
     4801 ACCTCAGCAG TTACAAGCCT TCAAAAATGA AGTAGGAGTA CTCAGGAAAA CACGACATGT 
     4861 GAATATCCTA CTCTTCATGG GCTATTCCAC AAAGCCACAA CTGGCTATTG TTACCCAGTG 
     4921 GTGTGAGGGC TCCAGCTTGT ATCACCATCT CCATATCATT GAGACCAAAT TTGAGATGAT 
     4981 CAAACTTATA GATATTGCAC GACAGACTGC ACAGGGCATG GATTACTTAC ACGCCAAGTC 
     5041 AATCATCCAC AGAGACCTCA AGAGTAATAA TATATTTCTT CATGAAGACC TCACAGTAAA 
     5101 AATAGGTGAT TTTGGTCTAG CTACAGTGAA ATCTCGATGG AGTGGGTCCC ATCAGTTTGA 
     5161 ACAGTTGTCT GGATCCATTT TGTGGATGGC ACCAGAAGTC ATCAGAATGC AAGATAAAAA 
     5221 TCCATACAGC TTTCAGTCAG ATGTATATGC ATTTGGAATT GTTCTGTATG AATTGATGAC 
     5281 TGGACAGTTA CCTTATTCAA ACATCAACAA CAGGGACCAG ATAATTTTTA TGGTGGGACG 
     5341 AGGATACCTG TCTCCAGATC TCAGTAAGGT ACGGAGTAAC TGTCCAAAAG CCATGAAGAG 
     5401 ATTAATGGCA GAGTGCCTCA AAAAGAAAAG AGATGAGAGA CCACTCTTTC CCCAAATTCT 
     5461 CGCCTCTATT GAGCTGCTGG CCCGCTCATT GCCAAAAATT CACCGCAGTG CATCAGAACC 
     5521 CTCCTTGAAT CGGGCTGGTT TCCAAACAGA GGATTTTAGT CTATATGCTT GTGCTTCTCC 
     5581 AAAAACACCC ATCCAGGCAG GGGGATATGG TGCGTTTCCT GTCCACACGC GTACGCGGCC 
     5641 GCTCGAGATG AGCGGGGGCG AGGAGCTGTT CGCCGGCATC GTGCCCGTGC TGATCGAGCT 
     5701 GGACGGCGAC GTGCACGGCC ACAAGTTCAG CGTGCGCGGC GAGGGCGAGG GCGACGCCGA 
     5761 CTACGGCAAG CTGGAGATCA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC 
     5821 CACCCTGGTG ACCACCCTCT GCTACGGCAT CCAGTGCTTC GCCCGCTACC CCGAGCACAT 
     5881 GAAGATGAAC GACTTCTTCA AGAGCGCCAT GCCCGAGGGC TACATCCAGG AGCGCACCAT 
     5941 CCAGTTCCAG GACGACGGCA AGTACAAGAC CCGCGGCGAG GTGAAGTTCG AGGGCGACAC 
     6001 CCTGGTGAAC CGCATCGAGC TGAAGGGCAA GGACTTCAAG GAGGACGGCA ACATCCTGGG 
     6061 CCACAAGCTG GAGTACAGCT TCAACAGCCA CAACGTGTAC ATCCGCCCCG ACAAGGCCAA 
     6121 CAACGGCCTG GAGGCTAACT TCAAGACCCG CCACAACATC GAGGGCGGCG GCGTGCAGCT 
     6181 GGCCGACCAC TACCAGACCA ACGTGCCCCT GGGCGACGGC CCCGTGCTGA TCCCCATCAA 
     6241 CCACTACCTG AGCACTCAGA CCAAGATCAG CAAGGACCGC AACGAGGCCC GCGACCACAT 
     6301 GGTGCTCCTG GAGTCCTTCA GCGCCTGCTG CCACACCCAC GGCATGGACG AGCTGTACAG 
     6361 GTCCGGACTC AGATAAGTTT AAACGGCCGG CCGCGGTCTG TACAAGTAGG ATTCGTCGAG 
     6421 GGACCTAATA ACTTCGTATA GCATACATTA TACGAAGTTA TACATGTTTA AGGGTTCCGG 
     6481 TTCCACTAGG TACAATTCGA TATCAAGCTT ATCGATAATC AACCTCTGGA TTACAAAATT 
     6541 TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT 
     6601 GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG 
     6661 TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC 
     6721 GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT 
     6781 CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC 
     6841 GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG 
     6901 TTGTCGGGGA AATCATCGTC CTTTCCTTGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG 
     6961 CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC 
     7021 GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG 
     7081 ATCTCCCTTT GGGCCGCCTC CCCGCATCGA TACCGTCGAC CTCGATCGAG ACCTAGAAAA 
     7141 ACATGGAGCA ATCACAAGTA GCAATACAGC AGCTACCAAT GCTGATTGTG CCTGGCTAGA 
     7201 AGCACAAGAG GAGGAGGAGG TGGGTTTTCC AGTCACACCT CAGGTACCTT TAAGACCAAT 
     7261 GACTTACAAG GCAGCTGTAG ATCTTAGCCA CTTTTTAAAA GAAAAGGGGG GACTGGAAGG 
     7321 GCTAATTCAC TCCCAACGAA GACAAGATAT CCTTGATCTG TGGATCTACC ACACACAAGG 
     7381 CTACTTCCCT GATTGGCAGA ACTACACACC AGGGCCAGGG ATCAGATATC CACTGACCTT 
     7441 TGGATGGTGC TACAAGCTAG TACCAGTTGA GCAAGAGAAG GTAGAAGAAG CCAATGAAGG 
     7501 AGAGAACACC CGCTTGTTAC ACCCTGTGAG CCTGCATGGG ATGGATGACC CGGAGAGAGA 
     7561 AGTATTAGAG TGGAGGTTTG ACAGCCGCCT AGCATTTCAT CACATGGCCC GAGAGCTGCA 
     7621 TCCGGACTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA 
     7681 ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG 
     7741 TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG 
     7801 GAAAATCTCT AGCAGCATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     7861 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     7921 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     7981 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     8041 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     8101 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     8161 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     8221 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     8281 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     8341 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     8401 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     8461 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     8521 TTAAGGGATT TTGGTCATGA TTACGCCCCG CCCTGCCACT CATCGCAGTA CTGTTGTAAT 
     8581 TCATTAAGCA TTCTGCCGAC ATGGAAGCCA TCACAAACGG CATGATGAAC CTGAATCGCC 
     8641 AGCGGCATCA GCACCTTGTC GCCTTGCGTA TAATATTTGC CCATGGTGAA AACGGGGGCG 
     8701 AAGAAGTTGT CCATATTGGC CACGTTTAAA TCAAAACTGG TGAAACTCAC CCAGGGATTG 
     8761 GCTGAGACGA AAAACATATT CTCAATAAAC CCTTTAGGGA AATAGGCCAG GTTTTCACCG 
     8821 TAACACGCCA CATCTTGCGA ATATATGTGT AGAAACTGCC GGAAATCGTC GTGGTATTCA 
     8881 CTCCAGAGCG ATGAAAACGT TTCAGTTTGC TCATGGAAAA CGGTGTAACA AGGGTGAACA 
     8941 CTATCCCATA TCACCAGCTC ACCGTCTTTC ATTGCCATAC GGAACTCCGG ATGAGCATTC 
     9001 ATCAGGCGGG CAAGAATGTG AATAAAGGCC GGATAAAACT TGTGCTTATT TTTCTTTACG 
     9061 GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA ACGGTCTGGT TATAGGTACA TTGAGCAACT 
     9121 GACTGAAATG CCTCAAAATG TTCTTTACGA TGCCATTGGG ATATATCAAC GGTGGTATAT 
     9181 CCAGTGATTT TTTTCTCCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     9241 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     9301 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GAC
//