LOCUS       RC210990                8706 bp    DNA     circular  SYN 
DEFINITION  ATRN (Myc-DDK-tagged)-Human attractin (ATRN), transcript variant 2
ACCESSION   NM_139322
VERSION     NM_139322.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4861..4952 
                     /label="Myc-DDK"  
     misc_feature    4982..5567 
                     /label="PolyA signal"  
     misc_feature    5039..5058 
                     /label="Restriction Enzyme"  
     misc_feature    5843..6515 
                     /label="ColE1"  
     misc_feature    6945..7739 
                     /label="Kan/Neo"  
     misc_feature    7774..8076 
                     /label="SV40 ori"  
     misc_feature    8162..8639 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4836..4843 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4836 
                     /label="ATRN"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGGCTGCA GCGGCGGCAA CTGAGGCAAG GCTGAGGAGG AGGACGGCGG 
     1081 CGACGGCAGC GCTCGCGGGC AGGAGCGGCG GGCCGCACTG GGACTGGGAC GTGACCAGGG 
     1141 CTGGGAGGCC GGGGCTGGGG GCCGGGCTGC GCCTCCCGCG GCTGCTGTCT CCACCGCTGC 
     1201 GGCCACGGCT GCTGCTGCTG CTGTTGTTGC TCTCGCCGCC GCTGCTGCTG CTGCTGCTGC 
     1261 CCTGTGAGGC CGAGGCCGCC GCGGCGGCGG CGGCGGTGTC GGGCTCAGCC GCAGCCGAGG 
     1321 CCAAGGAATG TGACCGGCCC TGTGTCAACG GCGGTCGCTG CAACCCTGGC ACCGGCCAGT 
     1381 GCGTCTGCCC CGCCGGCTGG GTGGGCGAGC AATGCCAGCA CTGCGGGGGC CGCTTCAGAC 
     1441 TAACTGGATC TTCTGGGTTT GTGACAGATG GACCTGGAAA TTATAAATAC AAAACGAAGT 
     1501 GCACGTGGCT CATTGAAGGA CAGCCAAATA GAATAATGAG ACTTCGTTTC AATCATTTTG 
     1561 CTACAGAGTG TAGTTGGGAC CATTTATATG TTTATGATGG GGACTCAATT TATGCACCGC 
     1621 TAGTTGCTGC ATTTAGTGGC CTCATTGTTC CTGAGAGAGA TGGCAATGAG ACTGTCCCTG 
     1681 AGGTTGTTGC CACATCAGGT TATGCCTTGC TGCATTTTTT TAGTGATGCT GCTTATAATT 
     1741 TGACTGGATT TAATATTACT TACAGTTTTG ATATGTGTCC AAATAACTGC TCAGGCCGAG 
     1801 GAGAGTGTAA GATCAGTAAT AGCAGCGATA CTGTTGAATG TGAATGTTCT GAAAACTGGA 
     1861 AAGGTGAAGC ATGTGACATT CCTCACTGTA CAGACAACTG TGGTTTTCCT CATCGAGGCA 
     1921 TCTGCAATTC AAGTGATGTC AGAGGATGCT CCTGCTTCTC AGACTGGCAG GGTCCTGGAT 
     1981 GTTCAGTTCC TGTACCAGCT AACCAGTCAT TTTGGACTCG AGAGGAATAT TCTAACTTAA 
     2041 AGCTCCCCAG AGCATCTCAT AAAGCTGTGG TCAATGGAAA CATTATGTGG GTTGTTGGAG 
     2101 GATATATGTT CAACCACTCA GATTATAACA TGGTTCTAGC GTATGACCTT GCTTCTAGGG 
     2161 AGTGGCTTCC ACTAAACCGT TCTGTGAACA ATGTGGTTGT TAGATATGGT CATTCTTTGG 
     2221 CATTATACAA GGATAAAATT TACATGTATG GAGGAAAAAT TGATTCAACT GGGAATGTGA 
     2281 CCAATGAGTT GAGAGTTTTT CACATTCATA ATGAGTCATG GGTGTTGTTG ACCCCTAAGG 
     2341 CAAAGGAGCA GTATGCAGTG GTTGGGCACT CTGCACACAT TGTTACACTG AAGAATGGCC 
     2401 GAGTGGTCAT GCTGGTCATC TTTGGTCACT GCCCTCTCTA TGGATATATA AGCAATGTGC 
     2461 AGGAATATGA TTTGGATAAG AACACATGGA GTATATTACA CACCCAGGGT GCCCTTGTGC 
     2521 AAGGGGGTTA CGGCCATAGC AGTGTTTACG ACCATAGGAC CAGGGCCCTA TACGTTCATG 
     2581 GTGGCTACAA GGCTTTCAGT GCCAATAAGT ACCGGCTTGC AGATGATCTC TACCGATATG 
     2641 ATGTGGATAC CCAGATGTGG ACCATTCTTA AGGACAGCCG ATTTTTCCGT TACTTGCACA 
     2701 CAGCTGTGAT AGTGAGTGGA ACCATGCTGG TGTTTGGAGG AAACACACAC AATGACACAT 
     2761 CTATGAGCCA TGGCGCCAAA TGCTTCTCTT CAGATTTCAT GGCCTATGAC ATTGCCTGTG 
     2821 ACCGCTGGTC AGTGCTTCCC AGACCTGATC TCCACCATGA TGTCAACAGA TTTGGCCATT 
     2881 CAGCAGTCTT ACACAACAGC ACCATGTATG TGTTCGGTGG TTTCAATAGT CTCCTCCTCA 
     2941 GCGACATCCT GGTATTCACC TCGGAACAGT GTGATGCGCA TCGGAGTGAA GCCGCTTGTT 
     3001 TAGCAGCAGG ACCTGGTATT CGGTGTGTGT GGAACACAGG GTCGTCTCAG TGTATCTCGT 
     3061 GGGCGCTGGC AACTGATGAA CAAGAAGAAA AGTTAAAATC AGAATGTTTT TCCAAAAGAA 
     3121 CTCTTGACCA TGACAGATGT GACCAGCACA CAGATTGTTA CAGCTGCACA GCCAACACCA 
     3181 ATGACTGCCA CTGGTGCAAT GACCATTGTG TCCCCAGGAA CCACAGCTGC TCAGAAGGCC 
     3241 AGATCTCCAT TTTTAGGTAT GAGAATTGCC CCAAGGATAA CCCCATGTAC TACTGTAACA 
     3301 AGAAGACCAG CTGCAGGAGC TGTGCCCTGG ACCAGAACTG CCAGTGGGAG CCCCGGAATC 
     3361 AGGAGTGCAT TGCCCTGCCC GAAAATATCT GTGGCATTGG CTGGCATTTG GTTGGAAACT 
     3421 CATGTTTGAA AATTACTACT GCCAAGGAGA ATTATGACAA TGCTAAATTG TTCTGTAGGA 
     3481 ACCACAATGC CCTTTTGGCT TCTCTTACAA CCCAGAAGAA GGTAGAATTT GTCCTTAAGC 
     3541 AGCTGCGAAT AATGCAGTCA TCTCAGAGCA TGTCCAAGCT CACCTTAACC CCATGGGTCG 
     3601 GCCTTCGGAA GATCAATGTG TCCTACTGGT GCTGGGAAGA TATGTCCCCA TTTACAAATA 
     3661 GTTTACTACA GTGGATGCCG TCTGAGCCCA GTGATGCTGG ATTCTGTGGA ATTTTATCAG 
     3721 AACCCAGTAC TCGGGGACTG AAGGCTGCAA CCTGCATCAA CCCACTCAAT GGTAGTGTCT 
     3781 GTGAAAGGCC TGCAAACCAC AGTGCTAAGC AGTGCCGGAC ACCATGTGCC TTGAGGACAG 
     3841 CATGTGGAGA TTGCACCAGC GGCAGCTCTG AGTGCATGTG GTGCAGCAAC ATGAAGCAGT 
     3901 GTGTGGACTC CAATGCCTAT GTGGCCTCCT TCCCTTTTGG CCAGTGTATG GAATGGTATA 
     3961 CGATGAGCAC CTGCCCCCCT GAAAATTGTT CAGGCTACTG TACCTGTAGT CATTGCTTGG 
     4021 AGCAACCAGG CTGTGGCTGG TGTACTGATC CCAGCAATAC TGGCAAAGGG AAATGCATAG 
     4081 AGGGTTCCTA TAAAGGACCA GTGAAGATGC CTTCGCAAGC CCCTACAGGA AATTTCTATC 
     4141 CACAGCCCCT GCTCAATTCC AGCATGTGTC TAGAGGACAG CAGATACAAC TGGTCTTTCA 
     4201 TTCACTGTCC AGCTTGCCAA TGCAACGGCC ACAGTAAATG CATCAATCAG AGCATCTGTG 
     4261 AGAAGTGTGA GAACCTGACC ACAGGCAAGC ACTGCGAGAC CTGCATATCT GGCTTCTACG 
     4321 GTGATCCCAC CAATGGAGGG AAATGTCAGC CATGCAAGTG CAATGGGCAC GCGTCTCTGT 
     4381 GCAACACCAA CACGGGCAAG TGCTTCTGCA CCACCAAGGG CGTCAAGGGG GACGAGTGCC 
     4441 AGCTATGTGA GGTAGAAAAT CGATACCAAG GAAACCCTCT CAGAGGAACA TGTTATTATA 
     4501 CTCTTCTTAT TGACTATCAG TTCACCTTTA GTCTATCCCA GGAAGATGAT CGCTATTACA 
     4561 CAGCTATCAA TTTTGTGGCT ACTCCTGACG AACAAAACAG GGATTTGGAC ATGTTCATCA 
     4621 ATGCCTCCAA GAATTTCAAC CTCAACATCA CCTGGGCTGC CAGTTTCTCA GCTGGAACCC 
     4681 AGGCTGGAGA AGAGATGCCT GTTGTTTCAA AAACCAACAT TAAGGAGTAC AAAGATAGTT 
     4741 TCTCTAATGA GAAGTTTGAT TTTCGCAACC ACCCAAATAT CACTTTCTTT GTTTATGTCA 
     4801 GTAATTTCAC CTGGCCCATC AAAATTCAGG TGCAAACTGA ACAAAGCGGA CCGACGCGTA 
     4861 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4921 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4981 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     5041 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5101 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5161 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5221 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5281 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5341 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5401 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5461 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5521 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5581 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5641 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5701 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5761 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5821 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5881 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5941 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     6001 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6061 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6121 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6181 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6241 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6301 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6361 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6421 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6481 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6541 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6601 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6661 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6721 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6781 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6841 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6901 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6961 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     7021 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7081 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7141 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7201 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7261 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7321 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7381 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7441 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7501 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7561 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7621 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7681 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7741 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7801 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7861 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7921 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7981 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     8041 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8101 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8161 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8221 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8281 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8341 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8401 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8461 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8521 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8581 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8641 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8701 AATTTT
//