LOCUS       RC210936                7404 bp    DNA     circular  SYN 
DEFINITION  GRIPAP1 (Myc-DDK-tagged)-Human GRIP1 associated protein 1 (GRIPAP1), transcript variant 1
ACCESSION   NM_020137
VERSION     NM_020137.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3561..3652 
                     /label="Myc-DDK"  
     misc_feature    3682..4267 
                     /label="PolyA signal"  
     misc_feature    3739..3758 
                     /label="Restriction Enzyme"  
     misc_feature    4543..5215 
                     /label="ColE1"  
     misc_feature    5645..6439 
                     /label="Kan/Neo"  
     misc_feature    6474..6776 
                     /label="SV40 ori"  
     misc_feature    6862..7339 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3543..3549 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3543 
                     /label="GRIPAP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCAAGCT CTGTCTGAGG AGGAGTTTCA GCGGATGCAG GCTCAGCTCC 
     1081 TGGAACTCCG GACAAACAAC TACCAGCTTT CAGATGAACT ACGCAAGAAT GGTGTTGAAC 
     1141 TCACCAGTCT TCGACAGAAG GTCGCCTACT TGGATAAGGA GTTCAGCAAA GCTCAGAAGG 
     1201 CACTGAGCAA GAGCAAGAAA GCTCAGGAAG TCGAGGTATT GCTGAGTGAA AATGAGATGC 
     1261 TGCAGGCAAA GCTGCACAGC CAGGAGGAGG ACTTCCGTTT GCAGAACAGC ACACTAATGG 
     1321 CCGAGTTCAG CAAGCTCTGC AGCCAGATGG AACAGCTGGA GCAAGAGAAC CAGCAACTGA 
     1381 AGGAGGGGGC TGCAGGAGCA GGGGTTGCCC AAGCTGGGCC CCTCGTGGAT GGGGAGCTGC 
     1441 TGAGGCTACA GGCTGAAAAC ACAGCCTTGC AGAAGAACGT GGCAGCCCTG CAGGAACGCT 
     1501 ATGGGAAAGA AGCCGGGAAG TTCTCAGCTG TCAGTGAGGG CCAGGGGGAT CCCCCAGGGG 
     1561 GCCCGGCCCC CACCGTCCTG GCCCCCATGC CGTTGGCAGA GGTGGAGCTG AAATGGGAAA 
     1621 TGGAGAAAGA GGAGAAGAGA TTGCTCTGGG AACAGCTGCA AGGCTTAGAG AGCTCAAAGC 
     1681 AGGCCGAAAC ATCCAGGCTG CAGGAGGAAC TTGCTAAGCT CTCCGAGAAA CTGAAAAAGA 
     1741 AACAAGAAAG TTTTTGCCGT CTGCAGACAG AAAAGGAGAC TCTGTTTAAT GACAGCAGGA 
     1801 ACAAGATTGA GGAATTACAA CAACGGAAGG AAGCTGATCA CAAAGCCCAG TTGGCTCGAA 
     1861 CCCAGAAGCT GCAGCAGGAA CTTGAGGCTG CCAATCAGAG CTTGGCAGAG CTGAGAGATC 
     1921 AGCGGCAGGG GGAGCGCCTG GAACATGCAG CAGCTTTGCG GGCCCTACAA GATCAGGTAT 
     1981 CCATCCAGAG TGCAGATGCA CAGGAACAAG TGGAAGGGCT TTTGGCTGAG AACAATGCCT 
     2041 TGAGGACTAG CCTGGCTGCC CTGGAGCAGA TCCAAACAGC AAAGACCCAA GAACTGAATA 
     2101 TGCTCCGGGA ACAGACCACT GGGCTGGCAG CTGAGTTGCA GCAGCAGCAG GCTGAGTACG 
     2161 AGGACCTTAT GGGACAGAAA GATGACCTCA ACTCCCAGCT CCAGGAGTCA TTACGGGCCA 
     2221 ATAGTCGACT GCTGGAACAA CTTCAAGAAA TAGGGCAGGA GAAGGAGCAG TTGACCCAGG 
     2281 AATTACAGGA GGCTCGGAAG AGTGCGGAGA AGCGGAAGGC CATGCTGGAT GAGCTAGCAA 
     2341 TGGAAACGCT GCAAGAGAAG TCCCAGCACA AGGAAGAGCT GGGAGCAGTT CGTCTACGGC 
     2401 ATGAGAAGGA GGTGCTGGGG GTGCGTGCCC GCTATGAGCG TGAGCTCCGA GAGCTGCATG 
     2461 AAGACAAGAA GCGTCAGGAG GAGGAGCTCC GTGGGCAGAT CCGGGAGGAG AAGGCCCGGA 
     2521 CACGGGAGCT GGAGACTCTC CAGCAGACAG TGGAAGAACT TCAAGCTCAG GTACATTCCA 
     2581 TGGATGGAGC CAAGGGCTGG TTTGAACGGC GCTTGAAGGA AGCCGAGGAA TCCCTGCAGC 
     2641 AGCAGCAGCA GGAACAAGAG GAAGCCCTCA AGCAGTGTCG GGAGCAGCAC GCTGCCGAGC 
     2701 TGAAGGGCAA GGAGGAGGAG CTACAGGATG TACGGGATCA GCTCGAGCAG GCCCAGGAGG 
     2761 AGCGGGACTG CCACCTGAAG ACCATTAGCA GCCTGAAGCA GGAGGTGAAG GACACAGTGG 
     2821 ATGGGCAGAG GATCCTGGAG AAGAAGGGCA GTGCTGCGCT CAAGGACCTC AAGCGGCAGC 
     2881 TGCATTTGGA GCGGAAACGG GCAGATAAGC TGCAGGAGCG ACTGCAGGAC ATCCTCACTA 
     2941 ACAGCAAGAG CCGCTCAGGC CTTGAGGAGC TGGTTCTCTC AGAGATGAAC TCACCAAGCC 
     3001 GGACCCAGAC AGGGGACAGC AGTAGCATCT CCTCCTTCAG CTACCGGGAG ATCTTGCGGG 
     3061 AAAAGGAGAG CTCGGCTGTT CCAGCCAGGT CCTTATCCAG CAGCCCTCAA GCCCAGCCCC 
     3121 CTCGGCCAGC AGAGCTGTCA GATGAGGAAG TGGCTGAGCT CTTTCAGCGG CTGGCAGAGA 
     3181 CACAGCAGGA GAAATGGATG CTGGAGGAGA AGGTGAAGCA CCTGGAAGTG AGCAGTGCTT 
     3241 CCATGGCAGA GGACCTCTGC CGGAAGAGCG CCATCATTGA GACCTACGTC ATGGACAGCC 
     3301 GGATCGATGT GTCTGTGGCA GCAGGCCACA CAGACCGCAG CGGGCTGGGC AGCGTCCTGA 
     3361 GAGACCTAGT GAAGCCAGGC GACGAGAACC TTCGGGAGAT GAACAAGAAG CTGCAGAACA 
     3421 TGCTGGAGGA GCAGCTCACC AAGAATATGC ACTTGCACAA GGATATGGAA GTTCTGTCCC 
     3481 AGGAAATTGT GCGGCTCAGC AAGGAGTGCG TGGGGCCTCC TGACCCAGAC CTAGAGCCAG 
     3541 GAGAAACCAG CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3601 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3661 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3721 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3781 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3841 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3901 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3961 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4021 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4081 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4141 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4201 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4261 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4321 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4381 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4441 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4501 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4561 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4621 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4681 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4741 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4801 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4861 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     4921 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     4981 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5041 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5101 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5161 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5221 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5281 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5341 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5401 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5461 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5521 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5581 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5641 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5701 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5761 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5821 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     5881 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     5941 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6001 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6061 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6121 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6181 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6241 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6301 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6361 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6421 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6481 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6541 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6601 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6661 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6721 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6781 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6841 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     6901 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     6961 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7021 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7081 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7141 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7201 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7261 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7321 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7381 TAACAAAAAT TTAACGCGAA TTTT
//