LOCUS       RC210866                7125 bp    DNA     circular  SYN 
DEFINITION  SMURF2 (Myc-DDK-tagged)-Human SMAD specific E3 ubiquitin protein ligase 2 (SMURF2)
ACCESSION   NM_022739
VERSION     NM_022739.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3282..3373 
                     /label="Myc-DDK"  
     misc_feature    3403..3988 
                     /label="PolyA signal"  
     misc_feature    3460..3479 
                     /label="Restriction Enzyme"  
     misc_feature    4264..4936 
                     /label="ColE1"  
     misc_feature    5366..6160 
                     /label="Kan/Neo"  
     misc_feature    6195..6497 
                     /label="SV40 ori"  
     misc_feature    6583..7060 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3264..3270 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3264 
                     /label="SMURF2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTAACCCC GGAGGCCGGA GGAACGGGCC CGTCAAGCTG CGCCTGACAG 
     1081 TACTCTGTGC AAAAAACCTG GTGAAAAAGG ATTTTTTCCG ACTTCCTGAT CCATTTGCTA 
     1141 AGGTGGTGGT TGATGGATCT GGGCAATGCC ATTCTACAGA TACTGTGAAG AATACGCTTG 
     1201 ATCCAAAGTG GAATCAGCAT TATGACCTGT ATATTGGAAA GTCTGATTCA GTTACGATCA 
     1261 GTGTATGGAA TCACAAGAAG ATCCATAAGA AACAAGGTGC TGGATTTCTC GGTTGTGTTC 
     1321 GTCTTCTTTC CAATGCCATC AACCGCCTCA AAGACACTGG TTATCAGAGG TTGGATTTAT 
     1381 GCAAACTCGG GCCAAATGAC AATGATACAG TTAGAGGACA GATAGTAGTA AGTCTTCAGT 
     1441 CCAGAGACCG AATAGGCACA GGAGGACAAG TTGTGGACTG CAGTCGTTTA TTTGATAACG 
     1501 ATTTACCAGA CGGCTGGGAA GAAAGGAGAA CCGCCTCTGG AAGAATCCAG TATCTAAACC 
     1561 ATATAACAAG AACTACGCAA TGGGAGCGCC CAACACGACC GGCATCCGAA TATTCTAGCC 
     1621 CTGGCAGACC TCTTAGCTGC TTTGTTGATG AGAACACTCC AATTAGTGGA ACAAATGGTG 
     1681 CAACATGTGG ACAGTCTTCA GATCCCAGGC TGGCAGAGAG GAGAGTCAGG TCACAACGAC 
     1741 ATAGAAATTA CATGAGCAGA ACACATTTAC ATACTCCTCC AGACCTACCA GAAGGCTATG 
     1801 AACAGAGGAC AACGCAACAA GGCCAGGTGT ATTTCTTACA TACACAGACT GGTGTGAGCA 
     1861 CATGGCATGA TCCAAGAGTG CCCAGGGATC TTAGCAACAT CAATTGTGAA GAGCTTGGTC 
     1921 CATTGCCTCC TGGATGGGAG ATCCGTAATA CGGCAACAGG CAGAGTTTAT TTCGTTGACC 
     1981 ATAACAACAG AACAACACAA TTTACAGATC CTCGGCTGTC TGCTAACTTG CATTTAGTTT 
     2041 TAAATCGGCA GAACCAATTG AAAGACCAAC AGCAACAGCA AGTGGTATCG TTATGTCCTG 
     2101 ATGACACAGA ATGCCTGACA GTCCCAAGGT ACAAGCGAGA CCTGGTTCAG AAACTAAAAA 
     2161 TTTTGCGGCA AGAACTTTCC CAACAACAGC CTCAGGCAGG TCATTGCCGC ATTGAGGTTT 
     2221 CCAGGGAAGA GATTTTTGAG GAATCATATC GACAGGTCAT GAAAATGAGA CCAAAAGATC 
     2281 TCTGGAAGCG ATTAATGATA AAATTTCGTG GAGAAGAAGG CCTTGACTAT GGAGGCGTTG 
     2341 CCAGGGAATG GTTGTATCTC TTGTCACATG AAATGTTGAA TCCATACTAT GGCCTCTTCC 
     2401 AGTATTCAAG AGATGATATT TATACATTGC AGATCAATCC TGATTCTGCA GTTAATCCGG 
     2461 AACATTTATC CTATTTCCAC TTTGTTGGAC GAATAATGGG AATGGCTGTG TTTCATGGAC 
     2521 ATTATATTGA TGGTGGTTTC ACATTGCCTT TTTATAAGCA ATTGCTTGGG AAGTCAATTA 
     2581 CCTTGGATGA CATGGAGTTA GTAGATCCGG ATCTTCACAA CAGTTTAGTG TGGATACTTG 
     2641 AGAATGATAT TACAGGTGTT TTGGACCATA CCTTCTGTGT TGAACATAAT GCATATGGTG 
     2701 AAATTATTCA GCATGAACTT AAACCAAATG GCAAAAGTAT CCCTGTTAAT GAAGAAAATA 
     2761 AAAAAGAATA TGTCAGGCTC TATGTGAACT GGAGATTTTT ACGAGGCATT GAGGCTCAAT 
     2821 TCTTGGCTCT GCAGAAAGGA TTTAATGAAG TAATTCCACA ACATCTGCTG AAGACATTTG 
     2881 ATGAGAAGGA GTTAGAGCTC ATTATTTGTG GACTTGGAAA GATAGATGTT AATGACTGGA 
     2941 AGGTAAACAC CCGGTTAAAA CACTGTACAC CAGACAGCAA CATTGTCAAA TGGTTCTGGA 
     3001 AAGCTGTGGA GTTTTTTGAT GAAGAGCGAC GAGCAAGATT GCTTCAGTTT GTGACAGGAT 
     3061 CCTCTCGAGT GCCTCTGCAG GGCTTCAAAG CATTGCAAGG TGCTGCAGGC CCGAGACTCT 
     3121 TTACCATACA CCAGATTGAT GCCTGCACTA ACAACCTGCC GAAAGCCCAC ACTTGCTTCA 
     3181 ATCGAATAGA CATTCCACCC TATGAAAGCT ATGAAAAGCT ATATGAAAAG CTGCTAACAG 
     3241 CCATTGAAGA AACATGTGGA TTTGCTGTGG AAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3301 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3361 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     3421 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     3481 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     3541 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     3601 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     3661 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     3721 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     3781 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     3841 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     3901 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     3961 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4021 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4081 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4141 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4201 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4261 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4321 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4381 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     4441 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     4501 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     4561 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     4621 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     4681 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     4741 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     4801 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     4861 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     4921 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     4981 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5041 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5101 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5161 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5221 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5281 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     5341 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     5401 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     5461 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     5521 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     5581 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     5641 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     5701 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     5761 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     5821 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     5881 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     5941 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6001 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6061 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6121 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6181 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6241 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6301 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6361 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6421 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6481 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     6541 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     6601 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     6661 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     6721 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     6781 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     6841 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     6901 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     6961 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7021 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7081 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//