LOCUS       RC210817                8502 bp    DNA     circular  SYN 
DEFINITION  EGF (Myc-DDK-tagged)-Human epidermal growth factor (EGF), transcript variant 1
ACCESSION   NM_001963
VERSION     NM_001963.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4659..4750 
                     /label="Myc-DDK"  
     misc_feature    4780..5365 
                     /label="PolyA signal"  
     misc_feature    4837..4856 
                     /label="Restriction Enzyme"  
     misc_feature    5641..6313 
                     /label="ColE1"  
     misc_feature    6743..7537 
                     /label="Kan/Neo"  
     misc_feature    7572..7874 
                     /label="SV40 ori"  
     misc_feature    7960..8437 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4641..4647 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4641 
                     /label="EGF"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGCTCACT CTTATCATTC TGTTGCCAGT AGTTTCAAAA TTTAGTTTTG 
     1081 TTAGTCTCTC AGCACCGCAG CACTGGAGCT GTCCTGAAGG TACTCTCGCA GGAAATGGGA 
     1141 ATTCTACTTG TGTGGGTCCT GCACCCTTCT TAATTTTCTC CCATGGAAAT AGTATCTTTA 
     1201 GGATTGACAC AGAAGGAACC AATTATGAGC AATTGGTGGT GGATGCTGGT GTCTCAGTGA 
     1261 TCATGGATTT TCATTATAAT GAGAAAAGAA TCTATTGGGT GGATTTAGAA AGACAACTTT 
     1321 TGCAAAGAGT TTTTCTGAAT GGGTCAAGGC AAGAGAGAGT ATGTAATATA GAGAAAAATG 
     1381 TTTCTGGAAT GGCAATAAAT TGGATAAATG AAGAAGTTAT TTGGTCAAAT CAACAGGAAG 
     1441 GAATCATTAC AGTAACAGAT ATGAAAGGAA ATAATTCCCA CATTCTTTTA AGTGCTTTAA 
     1501 AATATCCTGC AAATGTAGCA GTTGATCCAG TAGAAAGGTT TATATTTTGG TCTTCAGAGG 
     1561 TGGCTGGAAG CCTTTATAGA GCAGATCTCG ATGGTGTGGG AGTGAAGGCT CTGTTGGAGA 
     1621 CATCAGAGAA AATAACAGCT GTGTCATTGG ATGTGCTTGA TAAGCGGCTG TTTTGGATTC 
     1681 AGTACAACAG AGAAGGAAGC AATTCTCTTA TTTGCTCCTG TGATTATGAT GGAGGTTCTG 
     1741 TCCACATTAG TAAACATCCA ACACAGCATA ATTTGTTTGC AATGTCCCTT TTTGGTGACC 
     1801 GTATCTTCTA TTCAACATGG AAAATGAAGA CAATTTGGAT AGCCAACAAA CACACTGGAA 
     1861 AGGACATGGT TAGAATTAAC CTCCATTCAT CATTTGTACC ACTTGGTGAA CTGAAAGTAG 
     1921 TGCATCCACT TGCACAACCC AAGGCAGAAG ATGACACTTG GGAGCCTGAG CAGAAACTTT 
     1981 GCAAATTGAG GAAAGGAAAC TGCAGCAGCA CTGTGTGTGG GCAAGACCTC CAGTCACACT 
     2041 TGTGCATGTG TGCAGAGGGA TACGCCCTAA GTCGAGACCG GAAGTACTGT GAAGATGTTA 
     2101 ATGAATGTGC TTTTTGGAAT CATGGCTGTA CTCTTGGGTG TAAAAACACC CCTGGATCCT 
     2161 ATTACTGCAC GTGCCCTGTA GGATTTGTTC TGCTTCCTGA TGGGAAACGA TGTCATCAAC 
     2221 TTGTTTCCTG TCCACGCAAT GTGTCTGAAT GCAGCCATGA CTGTGTTCTG ACATCAGAAG 
     2281 GTCCCTTATG TTTCTGTCCT GAAGGCTCAG TGCTTGAGAG AGATGGGAAA ACATGTAGCG 
     2341 GTTGTTCCTC ACCCGATAAT GGTGGATGTA GCCAGCTCTG CGTTCCTCTT AGCCCAGTAT 
     2401 CCTGGGAATG TGATTGCTTT CCTGGGTATG ACCTACAACT GGATGAAAAA AGCTGTGCAG 
     2461 CTTCAGGACC ACAACCATTT TTGCTGTTTG CCAATTCTCA AGATATTCGA CACATGCATT 
     2521 TTGATGGAAC AGACTATGGA ACTCTGCTCA GCCAGCAGAT GGGAATGGTT TATGCCCTAG 
     2581 ATCATGACCC TGTGGAAAAT AAGATATACT TTGCCCATAC AGCCCTGAAG TGGATAGAGA 
     2641 GAGCTAATAT GGATGGTTCC CAGCGAGAAA GGCTTATTGA GGAAGGAGTA GATGTGCCAG 
     2701 AAGGTCTTGC TGTGGACTGG ATTGGCCGTA GATTCTATTG GACAGACAGA GGGAAATCTC 
     2761 TGATTGGAAG GAGTGATTTA AATGGGAAAC GTTCCAAAAT AATCACTAAG GAGAACATCT 
     2821 CTCAACCACG AGGAATTGCT GTTCATCCAA TGGCCAAGAG ATTATTCTGG ACTGATACAG 
     2881 GGATTAATCC ACGAATTGAA AGTTCTTCCC TCCAAGGCCT TGGCCGTCTG GTTATAGCCA 
     2941 GCTCTGATCT AATCTGGCCC AGTGGAATAA CGATTGACTT CTTAACTGAC AAGTTGTACT 
     3001 GGTGCGATGC CAAGCAGTCT GTGATTGAAA TGGCCAATCT GGATGGTTCA AAACGCCGAA 
     3061 GACTTACCCA GAATGATGTA GGTCACCCAT TTGCTGTAGC AGTGTTTGAG GATTATGTGT 
     3121 GGTTCTCAGA TTGGGCTATG CCATCAGTAA TAAGAGTAAA CAAGAGGACT GGCAAAGATA 
     3181 GAGTACGTCT CCAAGGCAGC ATGCTGAAGC CCTCATCACT GGTTGTGGTT CATCCATTGG 
     3241 CAAAACCAGG AGCAGATCCC TGCTTATATC AAAACGGAGG CTGTGAACAT ATTTGCAAAA 
     3301 AGAGGCTTGG AACTGCTTGG TGTTCGTGTC GTGAAGGTTT TATGAAAGCC TCAGATGGGA 
     3361 AAACGTGTCT GGCTCTGGAT GGTCATCAGC TGTTGGCAGG TGGTGAAGTT GATCTAAAGA 
     3421 ACCAAGTAAC ACCATTGGAC ATCTTGTCCA AGACTAGAGT GTCAGAAGAT AACATTACAG 
     3481 AATCTCAACA CATGCTAGTG GCTGAAATCA TGGTGTCAGA TCAAGATGAC TGTGCTCCTG 
     3541 TGGGATGCAG CATGTATGCT CGGTGTATTT CAGAGGGAGA GGATGCCACA TGTCAGTGTT 
     3601 TGAAAGGATT TGCTGGGGAT GGAAAACTAT GTTCTGATAT AGATGAATGT GAGATGGGTG 
     3661 TCCCAGTGTG CCCCCCTGCC TCCTCCAAGT GCATCAACAC CGAAGGTGGT TATGTCTGCC 
     3721 GGTGCTCAGA AGGCTACCAA GGAGATGGGA TTCACTGTCT TGATATTGAT GAGTGCCAAC 
     3781 TGGGGGTGCA CAGCTGTGGA GAGAATGCCA GCTGCACAAA TACAGAGGGA GGCTATACCT 
     3841 GCATGTGTGC TGGACGCCTG TCTGAACCAG GACTGATTTG CCCTGACTCT ACTCCACCCC 
     3901 CTCACCTCAG GGAAGATGAC CACCACTATT CCGTAAGAAA TAGTGACTCT GAATGTCCCC 
     3961 TGTCCCACGA TGGGTACTGC CTCCATGATG GTGTGTGCAT GTATATTGAA GCATTGGACA 
     4021 AGTATGCATG CAACTGTGTT GTTGGCTACA TCGGGGAGCG ATGTCAGTAC CGAGACCTGA 
     4081 AGTGGTGGGA ACTGCGCCAC GCTGGCCACG GGCAGCAGCA GAAGGTCATC GTGGTGGCTG 
     4141 TCTGCGTGGT GGTGCTTGTC ATGCTGCTCC TCCTGAGCCT GTGGGGGGCC CACTACTACA 
     4201 GGACTCAGAA GCTGCTATCG AAAAACCCAA AGAATCCTTA TGAGGAGTCG AGCAGAGATG 
     4261 TGAGGAGTCG CAGGCCTGCT GACACTGAGG ATGGGATGTC CTCTTGCCCT CAACCTTGGT 
     4321 TTGTGGTTAT AAAAGAACAC CAAGACCTCA AGAATGGGGG TCAACCAGTG GCTGGTGAGG 
     4381 ATGGCCAGGC AGCAGATGGG TCAATGCAAC CAACTTCATG GAGGCAGGAG CCCCAGTTAT 
     4441 GTGGAATGGG CACAGAGCAA GGCTGCTGGA TTCCAGTATC CAGTGATAAG GGCTCCTGTC 
     4501 CCCAGGTAAT GGAGCGAAGC TTTCATATGC CCTCCTATGG GACACAGACC CTTGAAGGGG 
     4561 GTGTCGAGAA GCCCCATTCT CTCCTATCAG CTAACCCATT ATGGCAACAA AGGGCCCTGG 
     4621 ACCCACCACA CCAAATGGAG CTGACTCAGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4681 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4741 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4801 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4861 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4921 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4981 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5041 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5101 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5161 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5221 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5281 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5341 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5401 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5461 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5521 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5581 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5641 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5701 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5761 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5821 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5881 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5941 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6001 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6061 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6121 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6181 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6241 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6301 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6361 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6421 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6481 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6541 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6601 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6661 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6721 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6781 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6841 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6901 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6961 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7021 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7081 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7141 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7201 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7261 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7321 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7381 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7441 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7501 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7561 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7621 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7681 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7741 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7801 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7861 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7921 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7981 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8041 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8101 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8161 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8221 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8281 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8341 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8401 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8461 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//