LOCUS       RC210714                6681 bp    DNA     circular  SYN 
DEFINITION  MED26 (Myc-DDK-tagged)-Human mediator complex subunit 26 (MED26)
ACCESSION   NM_004831
VERSION     NM_004831.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2838..2929 
                     /label="Myc-DDK"  
     misc_feature    2959..3544 
                     /label="PolyA signal"  
     misc_feature    3016..3035 
                     /label="Restriction Enzyme"  
     misc_feature    3820..4492 
                     /label="ColE1"  
     misc_feature    4922..5716 
                     /label="Kan/Neo"  
     misc_feature    5751..6053 
                     /label="SV40 ori"  
     misc_feature    6139..6616 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2820..2826 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2820 
                     /label="MED26"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACAGCGGCT CCGGCGTCTC CGCAGCAGAT CAGGGACCGG CTGCTGCAGG 
     1081 CCATCGACCC CCAGAGCAAC ATCCGGAACA TGGTGGCGGT GCTGGAAGTC ATCTCCAGCC 
     1141 TGGAGAAATA CCCTATTACC AAAGAGGCAC TTGAGGAAAC ACGACTTGGG AAGCTCATCA 
     1201 ACGACGTCCG CAAGAAAACC AAGAACGAGG AGCTCGCCAA GCGGGCCAAG AAGCTGCTGC 
     1261 GGAGCTGGCA GAAGCTCATC GAGCCGGCAC ACCAGCATGA GGCGGCGCTG CGGGGGCTGG 
     1321 CGGGGGCCAC CGGCTCTGCC AACGGGGGCG CACACAACTG CCGGCCGGAG GTGGGGGCGG 
     1381 CTGGCCCACC CAGGAGCATC CATGACCTGA AGAGCCGCAA TGACCTCCAG AGGCTGCCCG 
     1441 GGCAGCGGCT GGACAGGCTG GGCAGCCGCA AGCGCCGGGG TGACCAGCGT GACCTCGGCC 
     1501 ACCCAGGGCC GCCACCCAAG GTCTCCAAAG CTAGCCACGA CCCCCTGGTC CCCAACTCAT 
     1561 CCCCCCTCCC CACCAACGGG ATCAGTGGGA GTCCAGAGAG CTTCGCCAGC TCCCTGGATG 
     1621 GCAGTGGGCA TGCAGGCCCA GAGGGCAGCC GCCTGGAGCG TGACGAGAAT GACAAGCACA 
     1681 GTGGCAAGAT CCCCGTCAAC GCCGTGCGAC CGCACACCAG CTCCCCGGGC CTGGGCAAGC 
     1741 CCCCTGGACC CTGCTTGCAG CCAAAGGCTT CGGTGCTGCA GCAGCTGGAC AGGGTGGACG 
     1801 AGACTCCGGG GCCTCCCCAT CCCAAGGGAC CCCCTCGCTG CTCTTTCAGT CCTCGGAACT 
     1861 CACGGCATGA GGGCTCCTTT GCCCGGCAGC AGAGCTTGTA TGCACCCAAG GGCTCCGTGC 
     1921 CCAGCCCCTC ACCGCGGCCC CAGGCACTCG ATGCCACACA GGTGCCGTCA CCGCTTCCAC 
     1981 TGGCACAGCC GTCCACACCC CCCGTACGGC GGCTCGAGCT GCTGCCCAGT GCGGAAAGCC 
     2041 CAGTGTGCTG GCTTGAGCAG CCTGAGAGCC ACCAGCGGCT GGCGGGGCCG GGCTGCAAGG 
     2101 CAGGGCTGTC CCCAGCCGAG CCCCTCCTGT CCCGGGCAGG CTTTTCCCCA GACTCCTCCA 
     2161 AGGCGGACAG TGATGCTGCC TCCTCAGGGG GCTCGGACAG TAAAAAGAAG AAGAGGTACC 
     2221 GACCTCGAGA CTATACGGTT AACTTGGACG GGCAGGTGGC TGAGGCGGGC GTCAAGCCTG 
     2281 TCCGGTTAAA AGAGCGGAAG CTCACCTTTG ACCCCATGAC GAGACAGATC AAACCTCTGA 
     2341 CCCAGAAAGA GCCAGTGCGG GCAGACAGCC CTGTGCACAT GGAGCAGCAG TCCAGGACAG 
     2401 AGCTGGACAA GCAGGAGGCC AAGGCCAGCC TCCAGAGCCC CTTCGAACAG ACGAACTGGA 
     2461 AGGAGCTGTC ACGCAACGAG ATCATCCAGT CCTACCTGAG CCGGCAGAGC AGCCTGCTCT 
     2521 CATCATCGGG CGCGCAGACC CCAGGGGCTC ACCACTTCAT GTCTGAGTAC CTGAAGCAGG 
     2581 AGGAGAGCAC CCGGCAAGGG GCCAGGCAGC TGCATGTGCT GGTGCCTCAA AGCCCGCCCA 
     2641 CGGACCTCCC TGGTCTGACC CGGGAGGTCA CACAGGACGA TCTCGACAGA ATCCAGGCCA 
     2701 GCCAGTGGCC GGGGGTGAAC GGGTGTCAGG ACACACAGGG TAACTGGTAT GACTGGACGC 
     2761 AGTGCATATC GCTCGATCCG CACGGCGACG ACGGGCGCTT GAACATTCTG CCTTATGTCT 
     2821 GCTTGGACAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     2881 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     2941 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3001 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     3061 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     3121 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     3181 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     3241 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     3301 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     3361 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     3421 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     3481 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     3541 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     3601 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     3661 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     3721 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     3781 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     3841 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     3901 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     3961 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     4021 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     4081 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     4141 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     4201 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     4261 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     4321 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     4381 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     4441 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     4501 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     4561 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     4621 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     4681 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     4741 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     4801 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     4861 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     4921 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     4981 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     5041 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     5101 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     5161 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     5221 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     5281 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     5341 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     5401 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     5461 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     5521 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     5581 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     5641 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     5701 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     5761 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     5821 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     5881 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     5941 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6001 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6061 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     6121 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     6181 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     6241 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     6301 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     6361 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     6421 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     6481 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     6541 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     6601 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     6661 CAAAAATTTA ACGCGAATTT T
//