LOCUS       RC210659                7674 bp    DNA     circular  SYN 
DEFINITION  TPO (Myc-DDK-tagged)-Human thyroid peroxidase (TPO), transcript variant 1
ACCESSION   NM_000547
VERSION     NM_000547.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3829..3920 
                     /label="Myc-DDK"  
     misc_feature    3950..4535 
                     /label="PolyA signal"  
     misc_feature    4007..4026 
                     /label="Restriction Enzyme"  
     misc_feature    4811..5483 
                     /label="ColE1"  
     misc_feature    5913..6707 
                     /label="Kan/Neo"  
     misc_feature    6742..7044 
                     /label="SV40 ori"  
     misc_feature    7130..7607 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3819..3827 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3819 
                     /label="TPO"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGAGCGCTG GCTGTGCTGT CTGTCACGCT GGTTATGGCC TGCACAGAAG 
     1081 CCTTCTTCCC CTTCATCTCG AGAGGGAAAG AACTCCTTTG GGGAAAGCCT GAGGAGTCTC 
     1141 GTGTCTCTAG CGTCTTGGAG GAAAGCAAGC GCCTGGTGGA CACCGCCATG TACGCCACGA 
     1201 TGCAGAGAAA CCTCAAGAAA AGAGGAATCC TTTCTGCAGC TCAGCTTCTG TCTTTTTCCA 
     1261 AACTTCCTGA GCCAACAAGC GGAGTGATTG CCCGAGCAGC AGAGATAATG GAAACATCAA 
     1321 TACAAGCGAT GAAAAGAAAA GTCAACCTGA AAACTCAACA ATCACAGCAT CCAACGGATG 
     1381 CTTTATCAGA AGATCTGCTG AGCATCATTG CAAACATGTC TGGATGTCTC CCTTACATGC 
     1441 TGCCCCCAAA ATGCCCAAAC ACTTGCCTGG CGAACAAATA CAGGCCCATC ACAGGAGCTT 
     1501 GCAACAACAG AGACCACCCC AGATGGGGCG CCTCCAACAC GGCCCTGGCA CGATGGCTCC 
     1561 CTCCAGTCTA TGAGGACGGC TTCAGTCAGC CCCGAGGCTG GAACCCCGGC TTCTTGTACA 
     1621 ACGGGTTCCC ACTGCCCCCG GTCCGGGAGG TGACAAGACA TGTCATTCAA GTTTCAAATG 
     1681 AGGTTGTCAC AGATGATGAC CGCTATTCTG ACCTCCTGAT GGCATGGGGA CAATACATCG 
     1741 ACCACGACAT CGCGTTCACA CCACAGAGCA CCAGCAAAGC TGCCTTCGGG GGAGGGGCTG 
     1801 ACTGCCAGAT GACTTGTGAG AACCAAAACC CATGTTTTCC CATACAACTC CCGGAGGAGG 
     1861 CCCGGCCGGC CGCGGGCACC GCCTGTCTGC CCTTCTACCG CTCTTCGGCC GCCTGCGGCA 
     1921 CCGGGGACCA AGGCGCGCTC TTTGGGAACC TGTCCACGGC CAACCCGCGG CAGCAGATGA 
     1981 ACGGGTTGAC CTCGTTCCTG GACGCGTCCA CCGTGTATGG CAGCTCCCCG GCCCTAGAGA 
     2041 GGCAGCTGCG GAACTGGACC AGTGCCGAAG GGCTGCTCCG CGTCCACGCG CGCCTCCGGG 
     2101 ACTCCGGCCG CGCCTACCTG CCCTTCGTGC CGCCACGCGC GCCTGCGGCC TGTGCGCCCG 
     2161 AGCCCGGCAT CCCCGGAGAG ACCCGCGGGC CCTGCTTCCT GGCCGGAGAC GGCCGCGCCA 
     2221 GCGAGGTCCC CTCCCTGACG GCACTGCACA CGCTGTGGCT GCGCGAGCAC AACCGCCTGG 
     2281 CCGCGGCGCT CAAGGCCCTC AATGCGCACT GGAGCGCGGA CGCCGTGTAC CAGGAGGCGC 
     2341 GCAAGGTCGT GGGCGCTCTG CACCAGATCA TCACCCTGAG GGATTACATC CCCAGGATCC 
     2401 TGGGACCCGA GGCCTTCCAG CAGTACGTGG GTCCCTATGA AGGCTATGAC TCCACCGCCA 
     2461 ACCCCACTGT GTCCAACGTG TTCTCCACAG CCGCCTTCCG CTTCGGCCAT GCCACGATCC 
     2521 ACCCGCTGGT GAGGAGGCTG GACGCCAGCT TCCAGGAGCA CCCCGACCTG CCCGGGCTGT 
     2581 GGCTGCACCA GGCTTTCTTC AGCCCATGGA CATTACTCCG TGGAGGTGGT TTGGACCCAC 
     2641 TAATACGAGG CCTTCTTGCA AGACCAGCCA AACTGCAGGT GCAGGATCAG CTGATGAACG 
     2701 AGGAGCTGAC GGAAAGGCTC TTTGTGCTGT CCAATTCCAG CACCTTGGAT CTGGCGTCCA 
     2761 TCAACCTGCA GAGGGGCCGG GACCACGGGC TGCCAGGTTA CAATGAGTGG AGGGAGTTCT 
     2821 GCGGCCTGCC TCGCCTGGAG ACCCCCGCTG ACCTGAGCAC AGCCATCGCC AGCAGGAGCG 
     2881 TGGCCGACAA GATCCTGGAC TTGTACAAGC ATCCTGACAA CATCGATGTC TGGCTGGGAG 
     2941 GCTTAGCTGA AAACTTCCTC CCCAGGGCTC GGACAGGGCC CCTGTTTGCC TGTCTCATTG 
     3001 GGAAGCAGAT GAAGGCTCTG CGGGACGGTG ACTGGTTTTG GTGGGAGAAC AGCCACGTCT 
     3061 TCACGGATGC ACAGAGGCGT GAGCTGGAGA AGCACTCCCT GTCTCGGGTC ATCTGTGACA 
     3121 ACACTGGCCT CACCAGGGTG CCCATGGATG CCTTCCAAGT CGGCAAATTC CCCGAAGACT 
     3181 TTGAGTCTTG TGACAGCATC ACTGGCATGA ACCTGGAGGC CTGGAGGGAA ACCTTTCCTC 
     3241 AAGACGACAA GTGTGGCTTC CCAGAGAGCG TGGAGAATGG GGACTTTGTG CACTGTGAGG 
     3301 AGTCTGGGAG GCGCGTGCTG GTGTATTCCT GCCGGCACGG GTATGAGCTC CAAGGCCGGG 
     3361 AGCAGCTCAC TTGCACCCAG GAAGGATGGG ATTTCCAGCC TCCCCTCTGC AAAGATGTGA 
     3421 ACGAGTGTGC AGACGGTGCC CACCCCCCCT GCCACGCCTC TGCGAGGTGC AGAAACACCA 
     3481 AAGGCGGCTT CCAGTGTCTC TGCGCGGACC CCTACGAGTT AGGAGACGAT GGGAGAACCT 
     3541 GCGTAGACTC CGGGAGGCTC CCTCGGGTGA CTTGGATCTC CATGTCGCTG GCTGCTCTGC 
     3601 TGATCGGAGG CTTCGCAGGT CTCACCTCGA CGGTGATTTG CAGGTGGACA CGCACTGGCA 
     3661 CTAAATCCAC ACTGCCCATC TCGGAGACAG GCGGAGGAAC TCCCGAGCTG AGATGCGGAA 
     3721 AGCACCAGGC CGTAGGGACC TCACCGCAGC GGGCCGCAGC TCAGGACTCG GAGCAGGAGA 
     3781 GTGCTGGGAT GGAAGGCCGG GATACTCACA GGCTGCCGAG AGCCCTCACG CGGCCGCTCG 
     3841 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3901 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3961 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4021 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4081 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4141 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4201 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4261 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4321 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4381 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4441 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4501 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4561 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4621 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4681 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4741 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4801 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4861 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4921 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4981 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5041 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5101 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5161 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5221 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5281 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5341 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5401 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5461 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5521 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5581 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5641 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5701 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5761 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5821 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5881 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     5941 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6001 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6061 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6121 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6181 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6241 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6301 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6361 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6421 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6481 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6541 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6601 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6661 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6721 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6781 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6841 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6901 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6961 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7021 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7081 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7141 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7201 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7261 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7321 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7381 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7441 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7501 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7561 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7621 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
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