LOCUS       RC210596L1                9486 bp    DNA     circular  SYN 
DEFINITION  PITRM1 (Myc-DDK-tagged)-Human pitrilysin metallopeptidase 1 (PITRM1), nuclear gene encoding mitochondrial protein, transcript variant 2
ACCESSION   NM_014889
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7777..7957 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8685..9344 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    8116..8684 
                     /label="ColE1"  
     misc_feature    6652..6672 
                     /label="LR50 primer"  
     misc_feature    6449..6520 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6661..7252 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6431..6437 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6431 
                     /label="PITRM1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GTGGCGCTGC GGCGGGCGGC AGGGCCTGTG 
     3361 TGTGCTGAGG CGGCTGAGCG GCGGACATGC ACACCACAGA GCGTGGCGAT GGAACAGTAA 
     3421 CCGGGCTTGT GAGAGGGCTC TGCAGTATAA ACTAGGAGAC AAGATCCATG GATTCACCGT 
     3481 AAACCAGGTG ACATCTGTTC CCGAGCTGTT CCTGACTGCA GTGAAGCTCA CCCATGATGA 
     3541 CACAGGAGCC AGGTATTTAC ACCTGGCCAG AGAAGACACG AATAATCTGT TCAGCGTGCA 
     3601 GTTCCGTACC ACTCCCATGG ACAGTACTGG TGTTCCTCAC ATTCTTGAGC ATACTGTCCT 
     3661 TTGTGGGTCT CAGAAATATC CGTGCAGAGA CCCTTTCTTC AAAATGTTGA ACCGGTCCCT 
     3721 CTCCACGTTC ATGAACGCCT TCACAGCTAG TGATTATACT GTGTATCCAT TTTCCACACA 
     3781 AAATCCCAAG GACTTTCAGA ATCTCCTCTC GGTGTATTTG GATGCCACCT TTTTCCCATG 
     3841 TTTACGCGAG CTGGATTTCT GGCAGGAAGG ATGGCGGCTG GAACATGAGA ATCCGAGCGA 
     3901 CCCCCAGACG CCCTTGGTCT TTAAAGGAGT CGTCTTTAAT GAGATGAAGG GAGCGTTTAC 
     3961 AGACAATGAG AGGATATTCT CCCAGCACCT TCAGAACAGA CTTCTTCCCG ACCACACGTA 
     4021 CTCAGTGGTC TCCGGGGGTG ACCCACTGTG CATCCCGGAG CTTACATGGG AGCAGCTTAA 
     4081 GCAGTTTCAT GCCACTCACT ATCACCCAAG CAATGCTAGG TTCTTCACGT ACGGTAATTT 
     4141 TCCATTAGAA CAGCATCTGA AACAAATTCA CGAGGAAGCA CTGAGCAAAT TCCAGAAAAT 
     4201 TGAACCAAGC ACCGTGGTGC CAGCTCAGAC ACCCTGGGAC AAGCCTAGGG AATTCCAGAT 
     4261 AACATGTGGC CCGGATTCAT TTGCTACAGA TCCCTCTAAA CAAACAACCG TCAGCGTTAG 
     4321 CTTCCTCTTA CCGGACATCA CCGACACATT TGAAGCCTTC ACATTAAGTC TTCTGTCTTC 
     4381 ACTCTTGACT TCTGGGCCCA ATTCTCCCTT TTACAAAGCC TTGATTGAAT CTGGCCTTGG 
     4441 CACAGACTTT TCTCCTGATG TTGGATATAA TGGCTACACG AGGGAGGCCT ACTTTAGTGT 
     4501 CGGCCTCCAA GGGATTGTGG AGAAAGACAT TGAGACCGTC AGAAGCCTCA TAGACAGAAC 
     4561 GATTGATGAA GTAGTTGAGA AAGGATTTGA AGATGATCGA ATTGAGGCTT TACTTCATAA 
     4621 AATTGAAATA CAGATGAAAC ATCAGTCTAC CAGCTTTGGG CTGATGCTGA CATCATACAT 
     4681 AGCTTCTTGC TGGAACCATG ATGGGGACCC TGTGGAGCTC TTGAAGTTGG GAAATCAGTT 
     4741 AGCTAAATTC AGACAGTGCC TGCAGGAAAA TCCAAAATTT TTGCAAGAAA AAGTAAAACA 
     4801 GTATTTTAAG AATAACCAGC ATAAGCTGAC TTTATCGATG AGGCCAGATG ACAAGTATCA 
     4861 CGAGAAGCAG GCACAGGTGG AAGCCACGAA GCTCAAGCAG AAGGTCGAGG CTCTGTCCCC 
     4921 CGGAGACAGG CAGCAGATCT ACGAGAAAGG TCTAGAATTA CGGAGTCAAC AAAGCAAACC 
     4981 TCAAGATGCC TCTTGTCTGC CAGCGTTGAA AGTTTCCGAT ATTGAACCCA CCATACCTGT 
     5041 CACAGAGTTG GACGTGGTCC TGACAGCTGG AGATATCCCT GTTCAGTACT GCGCCCAGCC 
     5101 CACCAATGGC ATGGTGTATT TCCGGGCCTT CTCCAGCCTG AACACACTCC CCGAGGAGCT 
     5161 GAGGCCCTAT GTGCCCCTCT TCTGCAGCGT CCTCACCAAG CTGGGCTGCG GCCTTCTTGA 
     5221 CTACCGGGAG CAGGCTCAGC AGATAGAATT GAAGACCGGA GGGATGAGTG CTTCTCCCCA 
     5281 CGTGCTCCCC GACGACTCAC ACATGGACAC CTACGAGCAG GGTGTGCTTT TCTCCTCTCT 
     5341 CTGCCTGGAT CGAAACCTGC CAGACATGAT GCAGCTATGG AGTGAAATAT TTAACAACCC 
     5401 GTGCTTTGAA GAAGAGGAGC ACTTCAAGGT GCTGGTGAAG ATGACCGCCC AGGAGCTCGC 
     5461 CAATGGAATT CCTGACTCTG GGCACCTGTA CGCATCCATC AGGGCAGGCC GGACCCTCAC 
     5521 GCCCGCAGGG GACCTGCAGG AGACCTTCAG CGGGATGGAT CAGGTGCGGC TGATGAAGAG 
     5581 GATTGCAGAA ATGACAGATA TCAAACCCAT CCTGAGGAAG CTCCCACGTA TCAAGAAACA 
     5641 CTTGTTAAAT GGTGATAATA TGAGGTGTTC AGTGAATGCG ACTCCTCAGC AGATGCCTCA 
     5701 GACAGAAAAA GCGGTCGAAG ACTTCCTTAG AAGCATCGGT CGGAGTAAAA AGGAACGGAG 
     5761 GCCTGTGCGC CCACACACGG TCGAGAAACC TGTGCCCAGC AGCTCTGGTG GAGATGCCCA 
     5821 CGTTCCCCAT GGCTCCCAGG TCATTAGGAA GCTGGTCATG GAACCCACCT TCAAGCCCTG 
     5881 GCAGATGAAG ACTCACTTCC TGATGCCCTT CCCGGTGAAT TACGTGGGTG AATGCATCCG 
     5941 AACTGTCCCC TACACGGACC CAGATCATGC CAGTTTTGAA ATCCTTGCAC GTTTGATGAC 
     6001 TGCCAAATTC TTGCATACAG AAATTCGAGA AAAAGGCGGT GCTTATGGTG GAGGCGCAAA 
     6061 ACTCAGCCAC AATGGGATTT TCACCCTTTA CTCTTACAGG GACCCAAATA CAATAGAGAC 
     6121 GCTCCAGTCT TTTGGGAAGG CTGTCGACTG GGCTAAGTCT GGAAAATTCA CACAGCAAGA 
     6181 CATCGACGAA GCCAAACTTT CTGTCTTCTC AACCGTAGAT GCTCCTGTCG CTCCTTCAGA 
     6241 CAAAGGAATG GACCACTTCT TGTACGGCCT CTCGGATGAG ATGAAGCAGG CCCACAGAGA 
     6301 GCAGCTCTTT GCTGTCAGCC ACGACAAGCT CCTGGCCGTG AGCGATAGGT ACCTCGGCAC 
     6361 TGGGAAAAGC ACACACGGCC TGGCCATCCT CGGACCCGAG AACCCGAAAA TTGCCAAGGA 
     6421 CCCATCCTGG ATCATCCGAA CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA 
     6481 GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC 
     6541 CGGCCGCGGT CTGTACAAGT AGGATTCGTC GAGGGACCTA ATAACTTCGT ATAGCATACA 
     6601 TTATACGAAG TTATACATGT TTAAGGGTTC CGGTTCCACT AGGTACAATT CGATATCAAG 
     6661 CTTATCGATA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC 
     6721 TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT 
     6781 GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT 
     6841 GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA 
     6901 ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC 
     6961 CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG 
     7021 GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC GTCCTTTCCT 
     7081 TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT 
     7141 TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT 
     7201 CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCAT 
     7261 CGATACCGTC GACCTCGATC GAGACCTAGA AAAACATGGA GCAATCACAA GTAGCAATAC 
     7321 AGCAGCTACC AATGCTGATT GTGCCTGGCT AGAAGCACAA GAGGAGGAGG AGGTGGGTTT 
     7381 TCCAGTCACA CCTCAGGTAC CTTTAAGACC AATGACTTAC AAGGCAGCTG TAGATCTTAG 
     7441 CCACTTTTTA AAAGAAAAGG GGGGACTGGA AGGGCTAATT CACTCCCAAC GAAGACAAGA 
     7501 TATCCTTGAT CTGTGGATCT ACCACACACA AGGCTACTTC CCTGATTGGC AGAACTACAC 
     7561 ACCAGGGCCA GGGATCAGAT ATCCACTGAC CTTTGGATGG TGCTACAAGC TAGTACCAGT 
     7621 TGAGCAAGAG AAGGTAGAAG AAGCCAATGA AGGAGAGAAC ACCCGCTTGT TACACCCTGT 
     7681 GAGCCTGCAT GGGATGGATG ACCCGGAGAG AGAAGTATTA GAGTGGAGGT TTGACAGCCG 
     7741 CCTAGCATTT CATCACATGG CCCGAGAGCT GCATCCGGAC TGTACTGGGT CTCTCTGGTT 
     7801 AGACCAGATC TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA 
     7861 ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA 
     7921 CTAGAGATCC CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGCA TGTGAGCAAA 
     7981 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     8041 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     8101 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     8161 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     8221 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     8281 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     8341 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     8401 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     8461 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     8521 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     8581 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     8641 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGATTACGCC 
     8701 CCGCCCTGCC ACTCATCGCA GTACTGTTGT AATTCATTAA GCATTCTGCC GACATGGAAG 
     8761 CCATCACAAA CGGCATGATG AACCTGAATC GCCAGCGGCA TCAGCACCTT GTCGCCTTGC 
     8821 GTATAATATT TGCCCATGGT GAAAACGGGG GCGAAGAAGT TGTCCATATT GGCCACGTTT 
     8881 AAATCAAAAC TGGTGAAACT CACCCAGGGA TTGGCTGAGA CGAAAAACAT ATTCTCAATA 
     8941 AACCCTTTAG GGAAATAGGC CAGGTTTTCA CCGTAACACG CCACATCTTG CGAATATATG 
     9001 TGTAGAAACT GCCGGAAATC GTCGTGGTAT TCACTCCAGA GCGATGAAAA CGTTTCAGTT 
     9061 TGCTCATGGA AAACGGTGTA ACAAGGGTGA ACACTATCCC ATATCACCAG CTCACCGTCT 
     9121 TTCATTGCCA TACGGAACTC CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG 
     9181 GCCGGATAAA ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC 
     9241 TGAACGGTCT GGTTATAGGT ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA 
     9301 CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATACTCTTC 
     9361 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     9421 GAATGTATTT AGAAAAATAA ACAAATAGGG GTCCCGCGCA CATTTCCCCG AAAAGTGCCA 
     9481 CCTGAC
//