LOCUS       RC210546                8547 bp    DNA     circular  SYN 
DEFINITION  VCPIP1 (Myc-DDK-tagged)-Human valosin containing protein (p97)/p47 complex interacting protein 1 (VCPIP1)
ACCESSION   NM_025054
VERSION     NM_025054.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4704..4795 
                     /label="Myc-DDK"  
     misc_feature    4825..5410 
                     /label="PolyA signal"  
     misc_feature    4882..4901 
                     /label="Restriction Enzyme"  
     misc_feature    5686..6358 
                     /label="ColE1"  
     misc_feature    6788..7582 
                     /label="Kan/Neo"  
     misc_feature    7617..7919 
                     /label="SV40 ori"  
     misc_feature    8005..8482 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4686..4692 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4686 
                     /label="VCPIP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTCAGCCG CCGCCGCCGC CGCCTCCGTT GCCGCCGCCA CCTCCTCCCC 
     1081 CTGAGGCTCC ACAGACTCCG TCGTCCTTGG CGTCGGCGGC TGCTTCGGGG GGGCTTTTGA 
     1141 AGCGGAGAGA CCGGAGAATC CTTTCCGGGA GCTGCCCGGA TCCGAAGTGT CAGGCGCGTC 
     1201 TATTTTTCCC GGCCTCCGGT TCTGTCAGCA TCGAGTGTAC CGAGTGCGGC CAGCGGCACG 
     1261 AGCAGCAACA GCTGCTGGGG GTTGAGGAGG TGACCGACCC GGACGTAGTG CTACACAACC 
     1321 TGCTGCGGAA CGCGCTGCTC GGGGTTACGG GGGCACCCAA GAAGAACACG GAACTGGTAA 
     1381 AGGTGATGGG CCTTTCCAAC TATCACTGCA AATTGTTGTC GCCCATATTA GCTCGCTATG 
     1441 GAATGGACAA ACAGACAGGC CGGGCCAAGC TTCTCCGGGA CATGAACCAG GGCGAACTGT 
     1501 TCGATTGCGC CTTACTGGGT GACCGCGCCT TCCTCATAGA ACCAGAGCAT GTTAACACTG 
     1561 TGGGCTATGG CAAGGACCGC TCCGGAAGCC TCCTGTATTT GCATGACACT CTGGAGGACA 
     1621 TTAAGCGGGC CAATAAAAGC CAGGAATGTC TCATTCCAGT GCATGTGGAC GGGGATGGAC 
     1681 ACTGCTTGGT GCATGCTGTG TCTCGGGCTC TAGTAGGCCG AGAGCTCTTC TGGCATGCCT 
     1741 TAAGAGAGAA TCTTAAACAG CACTTTCAGC AGCACCTGGC CCGATATCAA GCTCTGTTCC 
     1801 ATGACTTCAT TGATGCTGCT GAGTGGGAGG ACATTATCAA TGAGTGTGAC CCTCTGTTTG 
     1861 TACCACCTGA GGGTGTTCCC TTGGGCCTGA GGAATATCCA CATATTTGGT CTTGCCAATG 
     1921 TGCTACATCG TCCTATTATT CTGTTAGATT CCCTCAGTGG CATGAGAAGC TCTGGTGATT 
     1981 ATTCAGCCAC CTTTCTACCT GGGCTCATCC CTGCAGAGAA GTGCACTGGG AAAGATGGTC 
     2041 ATTTGAACAA ACCAATCTGT ATTGCATGGA GCAGCTCCGG TAGAAACCAT TATATCCCCT 
     2101 TGGTAGGCAT AAAAGGGGCT GCTTTGCCCA AACTGCCTAT GAATTTGCTT CCTAAAGCAT 
     2161 GGGGTGTGCC TCAGGACCTT ATTAAAAAGT ACATAAAACT TGAAGAGGAT GGTGGTTGTG 
     2221 TTATTGGAGG TGACAGAAGT TTGCAAGATA AATACTTACT TAGGCTTGTT GCTGCTATGG 
     2281 AAGAAGTCTT TATGGACAAA CATGGTATCC ATCCTAGTTT GGTTGCTGAT GTCCATCAGT 
     2341 ATTTCTACAG AAGGACTGGA GTGATAGGAG TTCAGCCTGA GGAAGTCACA GCAGCTGCTA 
     2401 AAAAAGCAGT AATGGATAAT CGCCTTCACA AATGTTTGCT CTGTGGTGCC CTTTCTGAAC 
     2461 TTCATGTTCC TCCAGAGTGG TTGGCTCCTG GAGGGAAATT GTATAACCTG GCAAAAAGTA 
     2521 CTCATGGACA GCTGAGGACT GACAAAAATT ACAGCTTTCC CTTGAACAAT TTGGTTTGCT 
     2581 CATATGATTC AGTGAAAGAT GTTCTGGTAC CAGACTATGG AATGAGTAAC CTAACAGCTT 
     2641 GTAATTGGTG CCATGGCACA TCTGTGCGAA AGGTCAGAGG AGATGGGTCT ATTGTGTATT 
     2701 TGGATGGAGA CAGAACTAAT TCTAGGTCCA CTGGTGGCAA ATGTGGTTGT GGATTCAAAC 
     2761 ACTTTTGGGA TGGTAAGGAG TATGACAATC TACCAGAAGC TTTCCCTATT ACTTTAGAAT 
     2821 GGGGTGGAAG AGTGGTCAGA GAAACAGTAT ATTGGTTCCA GTATGAAAGT GATTCATCTT 
     2881 TGAATAGTAA TGTTTACGAT GTTGCAATGA AACTTGTTAC CAAGCACTTT CCAGGTGAAT 
     2941 TTGGGAGTGA AATCCTAGTT CAGAAAGTTG TCCACACTAT ATTGCATCAG ACTGCCAAAA 
     3001 AGAATCCCGA TGATTATACT CCTGTAAATA TAGATGGTGC TCACGCCCAA AGAGTTGGAG 
     3061 ATGTTCAAGG ACAAGAATCA GAGTCTCAGC TCCCAACTAA AATTATTCTT ACTGGACAGA 
     3121 AAACAAAAAC TTTGCACAAG GAGGAGTTAA ACATGAGTAA AACTGAAAGA ACTATTCAAC 
     3181 AGAATATTAC GGAACAGGCT TCTGTAATGC AGAAACGGAA AACAGAGAAG TTAAAACAAG 
     3241 AACAAAAAGG GCAACCCAGG ACTGTTTCTC CCAGTACCAT TCGTGATGGT CCATCCTCTG 
     3301 CACCTGCTAC ACCTACCAAG GCTCCCTATT CACCGACAAC TTCTAAGGAG AAGAAGATCC 
     3361 GAATCACAAC TAATGATGGA CGACAGTCCA TGGTTACCCT TAAGTCTTCA ACAACCTTTT 
     3421 TTGAACTTCA GGAAAGTATA GCCAGAGAAT TCAACATTCC TCCATATTTA CAGTGTATTC 
     3481 GATACGGGTT TCCTCCTAAA GAGTTAATGC CACCACAGGC AGGAATGGAA AAGGAACCAG 
     3541 TTCCTTTACA GCATGGCGAC AGAATTACAA TAGAAATTCT AAAAAGTAAA GCTGAAGGTG 
     3601 GTCAGTCTGC TGCAGCACAC TCAGCCCACA CTGTGAAACA AGAAGATATT GCTGTTACTG 
     3661 GTAAACTGTC ATCTAAGGAA CTTCAGGAGC AAGCTGAAAA AGAAATGTAC TCCTTGTGTC 
     3721 TTTTAGCAAC ATTAATGGGA GAAGATGTAT GGTCTTATGC AAAGGGACTT CCTCACATGT 
     3781 TTCAGCAGGG TGGTGTATTC TACAGTATTA TGAAGAAAAC CATGGGTATG GCTGATGGCA 
     3841 AGCATTGTAC TTTTCCACAT CTGCCTGGCA AAACCTTTGT CTATAATGCT TCTGAAGATA 
     3901 GACTGGAATT GTGTGTGGAT GCTGCAGGAC ATTTCCCCAT TGGTCCTGAT GTTGAAGATT 
     3961 TAGTTAAAGA GGCTGTAAGT CAGGTTCGAG CAGAGGCTAC TACAAGAAGT AGGGAATCAA 
     4021 GTCCCTCACA TGGGCTATTA AAACTAGGTA GTGGTGGAGT AGTGAAAAAG AAATCTGAGC 
     4081 AACTTCATAA CGTAACTGCC TTTCAGGGAA AAGGGCATTC TTTAGGAACT GCATCTGGTA 
     4141 ACCCACACCT TGATCCAAGA GCTAGGGAAA CTTCAGTTGT AAGAAAGCAT AATACAGGGA 
     4201 CAGACTTTAG TAATAGTTCC ACTAAAACAG AGCCTTCTGT ATTCACAGCT TCTTCTAGTA 
     4261 ATAGTGAGCT TATTCGAATA GCTCCTGGAG TAGTAACAAT GAGAGACGGC AGGCAGCTTG 
     4321 ATCCTGATTT GGTTGAGGCC CAGCGAAAAA AATTGCAGGA AATGGTTTCT TCTATTCAGG 
     4381 CTTCAATGGA CAGGCACCTT CGGGATCAAA GTACAGAGCA GTCACCATCT GATCTTCCTC 
     4441 AAAGGAAAAC AGAAGTTGTG AGTTCTTCTG CAAAGTCTGG GAGTCTTCAG ACTGGTTTGC 
     4501 CTGAATCTTT TCCTTTAACT GGTGGTACTG AAAATTTGAA TACAGAAACA ACTGATGGCT 
     4561 GTGTAGCAGA TGCACTGGGA GCAGCCTTTG CCACAAGGTC AAAAGCACAA AGGGGAAATT 
     4621 CCGTGGAGGA GCTTGAAGAG ATGGATAGTC AAGATGCTGA GATGACTAAC ACAACTGAGC 
     4681 CAATGGATCA CTCTACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4741 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4801 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4861 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4921 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4981 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5041 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5101 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5161 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5221 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5281 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5341 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5401 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5461 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5521 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5581 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5641 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5701 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5761 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5821 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5881 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5941 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6001 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6061 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6121 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6181 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6241 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6301 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6361 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6421 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6481 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6541 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6601 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6661 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6721 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6781 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6841 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6901 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6961 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7021 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7081 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7141 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7201 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7261 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7321 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7381 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7441 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7501 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7561 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7621 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7681 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7741 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7801 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7861 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7921 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7981 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8041 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8101 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8161 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8221 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8281 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8341 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8401 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8461 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8521 ATTTAACAAA AATTTAACGC GAATTTT
//