LOCUS       RC210545                7611 bp    DNA     circular  SYN 
DEFINITION  ANO6 (Myc-DDK-tagged)-Human anoctamin 6 (ANO6), transcript variant 1
ACCESSION   NM_001025356
VERSION     NM_001025356.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3768..3859 
                     /label="Myc-DDK"  
     misc_feature    3889..4474 
                     /label="PolyA signal"  
     misc_feature    3946..3965 
                     /label="Restriction Enzyme"  
     misc_feature    4750..5422 
                     /label="ColE1"  
     misc_feature    5852..6646 
                     /label="Kan/Neo"  
     misc_feature    6681..6983 
                     /label="SV40 ori"  
     misc_feature    7069..7546 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3750..3756 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3750 
                     /label="ANO6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAAAGATG AGCAGGAATG TTTTGCTACA AATGGAGGAG GAGGAGGACG 
     1081 ACGACGATGG GGATATCGTG TTGGAAAACC TTGGACAGAC AATTGTCCCC GATTTGGGAT 
     1141 CACTGGAAAG TCAGCATGAT TTTCGAACCC CGGAGTTTGA AGAATTTAAT GGAAAACCTG 
     1201 ACTCCCTCTT TTTTAATGAT GGCCAGCGAA GAATTGACTT TGTTCTAGTA TATGAGGATG 
     1261 AAAGCAGAAA AGAGACCAAT AAAAAGGGTA CAAATGAAAA ACAAAGGAGG AAAAGACAAG 
     1321 CATACGAATC TAACCTTATC TGTCATGGCC TGCAGTTAGA AGCAACAAGA TCAGTATTGG 
     1381 ATGACAAGCT TGTATTTGTA AAAGTACACG CACCATGGGA GGTGTTATGT ACGTATGCTG 
     1441 AGATAATGCA CATCAAATTG CCTCTGAAAC CCAATGATCT GAAAAACCGG TCCTCAGCCT 
     1501 TTGGTACACT CAACTGGTTT ACCAAAGTCC TCAGTGTAGA CGAAAGCATC ATCAAGCCAG 
     1561 AGCAAGAGTT TTTCACTGCC CCATTTGAGA AGAACCGGAT GAATGATTTT TACATAGTTG 
     1621 ATAGAGATGC TTTCTTCAAT CCAGCCACCA GAAGCCGCAT TGTTTACTTC ATCCTCTCTC 
     1681 GGGTCAAGTA TCAAGTGATA AACAATGTTA GCAAGTTTGG GATCAACAGA CTTGTAAACT 
     1741 CTGGGATCTA CAAGGCAGCT TTCCCACTCC ATGATTGCAA ATTCCGCCGT CAGTCAGAGG 
     1801 ATCCCAGCTG CCCTAATGAA CGGTACCTTC TGTACAGAGA ATGGGCTCAT CCTCGAAGCA 
     1861 TATACAAAAA GCAGCCCTTG GATCTTATCA GGAAATACTA TGGAGAGAAG ATTGGAATCT 
     1921 ACTTTGCTTG GCTGGGCTAT TACACTCAGA TGCTTCTCCT GGCCGCAGTT GTAGGAGTGG 
     1981 CTTGCTTTCT CTATGGATAT CTTAATCAAG ATAACTGTAC ATGGAGCAAA GAAGTTTGTC 
     2041 ATCCTGATAT TGGTGGCAAG ATCATAATGT GTCCTCAGTG TGATAGGCTT TGTCCATTCT 
     2101 GGAAACTCAA TATTACTTGC GAGTCCTCAA AGAAATTGTG CATCTTCGAC AGTTTTGGAA 
     2161 CCCTGGTCTT TGCAGTATTT ATGGGAGTAT GGGTTACCTT GTTTTTGGAG TTTTGGAAGC 
     2221 GACGCCAGGC AGAACTTGAG TATGAATGGG ATACTGTTGA GTTACAGCAG GAAGAACAAG 
     2281 CCCGACCAGA ATACGAAGCA CGATGTACTC ACGTAGTGAT AAATGAGATT ACTCAGGAAG 
     2341 AAGAACGCAT TCCCTTTACT GCCTGGGGAA AATGTATACG GATAACCCTC TGTGCCAGTG 
     2401 CTGTCTTTTT CTGGATCCTA TTGATCATCG CTTCAGTTAT TGGGATCATT GTCTATAGGC 
     2461 TCTCGGTGTT CATTGTATTT TCTGCAAAAC TTCCCAAGAA CATTAATGGA ACAGACCCAA 
     2521 TCCAGAAATA CCTGACTCCA CAGACAGCCA CGTCCATCAC GGCCTCCATC ATCAGCTTTA 
     2581 TAATTATCAT GATTCTGAAC ACCATATATG AAAAAGTGGC AATTATGATT ACTAACTTCG 
     2641 AACTCCCAAG GACCCAGACT GATTATGAGA ACAGCCTCAC CATGAAGATG TTCTTATTCC 
     2701 AGTTTGTCAA CTACTACTCT TCATGCTTCT ACATAGCATT CTTTAAGGGC AAATTTGTAG 
     2761 GCTATCCAGG AGACCCAGTT TATTGGTTGG GAAAATACAG AAATGAAGAG TGTGACCCAG 
     2821 GTGGCTGTCT TCTTGAACTG ACAACTCAGC TGACAATAAT CATGGGAGGA AAAGCAATCT 
     2881 GGAATAACAT ACAAGAAGTA TTATTGCCCT GGATCATGAA TCTAATTGGG CGATTTCACA 
     2941 GAGTTTCTGG ATCAGAAAAG ATAACCCCAC GATGGGAACA GGACTACCAT CTGCAGCCTA 
     3001 TGGGCAAACT GGGATTATTT TATGAATATC TTGAAATGAT TATTCAGTTT GGGTTCGTCA 
     3061 CCTTATTTGT GGCCTCTTTT CCACTGGCCC CTCTGTTGGC TCTCGTGAAC AATATATTGG 
     3121 AAATAAGAGT GGACGCATGG AAACTGACCA CCCAGTTTAG ACGCCTGGTA CCAGAGAAAG 
     3181 CCCAAGACAT TGGAGCATGG CAGCCCATCA TGCAAGGAAT AGCAATTCTG GCTGTGGTGA 
     3241 CCAATGCCAT GATCATAGCT TTCACGTCGG ACATGATCCC CCGCCTAGTG TACTACTGGT 
     3301 CCTTCTCCGT CCCTCCCTAC GGGGACCACA CTTCCTACAC CATGGAAGGG TACATCAACA 
     3361 ACACTCTCTC CATCTTCAAA GTCGCAGACT TCAAAAACAA AAGCAAGGGA AACCCGTACT 
     3421 CTGACCTGGG TAACCATACC ACATGCAGGT ATCGTGATTT CCGATACCCA CCTGGACACC 
     3481 CCCAGGAGTA TAAACACAAC ATCTACTATT GGCATGTGAT TGCAGCCAAG CTGGCTTTTA 
     3541 TCATTGTCAT GGAGCACGTC ATCTACTCTG TGAAATTTTT CATTTCATAT GCAATTCCCG 
     3601 ATGTATCAAA ACGCACAAAG AGCAAGATCC AGAGAGAAAA ATACCTAACC CAAAAGCTTC 
     3661 TTCATGAGAA TCACCTCAAA GATATGACGA AAAATATGGG GGTGATAGCT GAGCGGATGA 
     3721 TAGAAGCAGT AGATAACAAT TTACGGCCAA AATCAGAAAC GCGTACGCGG CCGCTCGAGC 
     3781 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     3841 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     3901 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     3961 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4021 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4081 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4141 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4201 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4261 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4321 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4381 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4441 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4501 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4561 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     4621 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4681 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4741 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     4801 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     4861 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     4921 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     4981 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5041 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5101 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5161 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5221 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5281 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5341 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5401 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     5461 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     5521 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     5581 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     5641 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     5701 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     5761 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     5821 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     5881 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     5941 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6001 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6061 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6121 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6181 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6241 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6301 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6361 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6421 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6481 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6541 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     6601 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6661 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6721 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     6781 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     6841 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6901 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     6961 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7021 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7081 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7141 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7201 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7261 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7321 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7381 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7441 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7501 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7561 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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