LOCUS       RC210522                9375 bp    DNA     circular  SYN 
DEFINITION  NCAPD3 (Myc-DDK-tagged)-Human non-SMC condensin II complex, subunit D3 (NCAPD3). Note: ORF is codon optimized
ACCESSION   NM_015261
VERSION     NM_015261.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5532..5623 
                     /label="Myc-DDK"  
     misc_feature    5653..6238 
                     /label="PolyA signal"  
     misc_feature    5710..5729 
                     /label="Restriction Enzyme"  
     misc_feature    6514..7186 
                     /label="ColE1"  
     misc_feature    7616..8410 
                     /label="Kan/Neo"  
     misc_feature    8445..8747 
                     /label="SV40 ori"  
     misc_feature    8833..9310 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5514..5520 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5514 
                     /label="NCAPD3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGGCGTTG CGGGGCCTTG GTAGCGGCCT GCAGCCCTGG TGTCCGCTGG 
     1081 ATCTTAGACT CGAATGGGTT GACACAGTGT GGGAACTGGA TTTCACAGAG ACTGAGCCTT 
     1141 TGGATCCCAG CATAGAAGCA GAGATCATAG AGACTGGATT GGCTGCATTC ACAAAACTCT 
     1201 ATGAAAGCCT TTTACCCTTT GCTACTGGAG AACATGGATC TATGGAGAGT ATCTGGACCT 
     1261 TCTTCATTGA GAACAATGTT TCCCATAGTA CACTGGTGGC ATTGTTCTAT CATTTTGTTC 
     1321 AAATAGTTCA TAAGAAGAAT GTCAGTGTAC AGTATCGAGA ATATGGCCTT CATGCCGCTG 
     1381 GGCTTTACTT TTTGCTACTA GAAGTACCAG GCAGTGTAGC CAATCAAGTA TTCCACCCAG 
     1441 TGATGTTTGA CAAATGCATT CAGACTCTAA AGAAGAGCTG GCCCCAGGAA TCTAACTTGA 
     1501 ATCGGAAAAG AAAGAAAGAA CAGCCTAAGA GCTCTCAGGC TAACCCCGGG AGGCATAGAA 
     1561 AAAGGGGAAA GCCACCCAGG AGAGAAGATA TTGAGATGGA TGAAATTATA GAAGAACAAG 
     1621 AAGATGAGAA TATTTGTTTT TCTGCCCGGG ACCTTTCTCA AATTCGAAAT GCCATCTTTC 
     1681 ACCTTTTAAA GAATTTTTTA AGGCTTCTGC CAAAGTTTTC CTTGAAAGAA AAGCCACAAT 
     1741 GTGTACAGAA TTGTATAGAG GTCTTTGTTT CATTAACTAA TTTTGAGCCA GTTCTTCATG 
     1801 AATGTCATGT TACACAAGCC AGAGCTCTTA ACCAAGCAAA ATACATACCA GAACTGGCTT 
     1861 ATTATGGATT GTATTTGCTG TGCTCTCCCA TTCATGGAGA AGGAGATAAG GTCATCAGTT 
     1921 GTGTTTTCCA TCAAATGCTC AGTGTAATAT TAATGTTAGA AGTTGGTGAA GGATCCCATC 
     1981 GTGCCCCCCT TGCTGTTACC TCCCAAGTCA TCAACTGTAG AAACCAGGCG GTCCAGTTTA 
     2041 TCAGCGCCCT TGTGGATGAA TTAAAGGAGA GTATATTCCC AGTCGTCCGT ATCTTACTGC 
     2101 AGCACATCTG TGCCAAGGTG GTAGATAAAT CAGAGTATCG TACTTTTGCA GCCCAGTCCC 
     2161 TAGTCCAGCT GCTCAGTAAA CTTCCTTGTG GGGAATACGC TATGTTCATT GCCTGGCTTT 
     2221 ACAAATACTC CCGAAGTTCC AAGATCCCAC ACCGGGTTTT TACTCTTGAT GTTGTCTTAG 
     2281 CTCTGTTAGA ACTGCCTGAA AGAGAGGTGG ATAACACCCT CTCCTTGGAG CATCAGAAGT 
     2341 TCTTAAAGCA TAAGTTCCTG GTGCAGGAAA TTATGTTTGA TCGTTGCTTA GACAAGGCGC 
     2401 CTACTGTCCG CAGCAAGGCA CTGTCCAGCT TTGCACACTG TCTGGAGTTG ACTGTTACCA 
     2461 GTGCGTCGGA GAGTATCCTG GAGCTCCTGA TTAACAGTCC TACGTTTTCT GTAATAGAGA 
     2521 GTCACCCTGG TACCTTACTG AGAAATTCAT CAGCTTTTTC CTACCAAAGG CAGACATCTA 
     2581 ACCGTTCCGA ACCCTCAGGG GAGATCAACA TAGACAGCAG TGGTGAAACA GTTGGATCTG 
     2641 GAGAAAGATG TGTCATGGCA ATGCTGAGAA GGAGGATCAG GGATGAGAAG ACCAACGTTA 
     2701 GGAAGTCTGC ACTGCAGGTA TTAGTGAGTA TTTTGAAACA CTGTGATGTC TCAGGCATGA 
     2761 AGGAAGACCT GTGGATTCTG CAGGACCAGT GTCGGGACCC TGCAGTGTCT GTCCGGAAGC 
     2821 AGGCCCTCCA GTCTCTTACT GAACTCCTTA TGGCTCAGCC TAGATGCGTG CAGATCCAGA 
     2881 AAGCCTGGTT GCGGGGGGTG GTCCCGGTGG TGATGGACTG CGAGAGCACT GTGCAGGAGA 
     2941 AGGCCCTGGA GTTCCTGGAC CAGCTGCTGC TGCAGAACAT CCGGCATCAC AGTCATTTTC 
     3001 ACTCTGGGGA CGACAGCCAG GTCCTCGCCT GGGCGCTTCT TACTCTCCTC ACCACCGAAA 
     3061 GCCAGGAACT GAGCCGATAT TTAAATAAGG CTTTTCATAT CTGGTCCAAG AAAGAAAAAT 
     3121 TCTCACCCAC TTTTATAAAC AATGTAATAT CTCACACTGG CACGGAACAT TCGGCACCTG 
     3181 CCTGGATGCT GCTCTCCAAG ATTGCTGGCT CCTCACCCAG GCTGGACTAC AGCAGAATAA 
     3241 TACAATCTTG GGAGAAAATC AGCAGTCAGC AGAATCCCAA TTCAAACACC TTAGGACATA 
     3301 TTCTCTGTGT GATTGGGCAT ATTGCAAAGC ATCTTCCTAA GAGCACCCGG GACAAAGTGA 
     3361 CTGATGCTGT CAAGTGTAAG CTGAATGGAT TTCAGTGGTC TCTAGAGGTG ATCAGTTCAG 
     3421 CTGTTGACGC CTTGCAGAGG CTTTGTAGAG CATCTGCAGA GACACCAGCA GAGGAGCAGG 
     3481 AATTGCTGAC GCAGGTGTGT GGGGATGTAC TCTCCACCTG CGAGCACCGC CTCTCCAACA 
     3541 TCGTTCTCAA GGAGAATGGA ACAGGGAATA TGGACGAAGA CCTGTTGGTG AAGTACATTT 
     3601 TTACCTTAGG GGATATAGCC CAGCTGTGTC CAGCCAGGGT GGAGAAGCGC ATCTTCCTTC 
     3661 TGATTCAGTC CGTCCTGGCT TCGTCTGCTG ATGCTGACCA CTCACCATCA TCTCAAGGCA 
     3721 GCAGTGAGGC CCCAGCGTCT CAGCCACCCC CCCAGGTCAG AGGTTCTGTC ATGCCCTCTG 
     3781 TGATTAGAGC ACATGCCATC ATTACCTTAG GTAAGCTGTG CTTACAGCAC GAGGATCTGG 
     3841 CAAAGAAGAG CATCCCAGCC CTGGTGCGAG AGCTCGAGGT GTGTGAGGAC GTGGCTGTCC 
     3901 GCAACAACGT CATCATTGTA ATGTGCGATC TCTGCATTCG CTACACCATC ATGGTGGACA 
     3961 AGTATATTCC CAACATCTCC ATGTGTCTGA AGGATTCCGA CCCATTCATC CGGAAGCAGA 
     4021 CACTCATCTT GCTTACCAAT CTCTTGCAGG AGGAATTTGT GAAATGGAAG GGCTCCCTGT 
     4081 TCTTCCGATT TGTCAGCACT CTGATCGATT CACACCCAGA CATTGCCAGC TTCGGGGAGT 
     4141 TTTGCCTGGC TCACCTGTTA CTGAAGAGGA ACCCTGTCAT GTTCTTCCAA CACTTCATTG 
     4201 AATGTATTTT TCACTTTAAT AACTATGAGA AGCATGAGAA GTACAACAAG TTCCCCCAGT 
     4261 CAGAGAGAGA GAAGCGGCTG TTTTCATTGA AGGGAAAGTC AAACAAAGAG AGACGAATGA 
     4321 AAATCTACAA ATTTCTTCTA GAGCACTTCA CAGATGAACA GCGATTCAAC ATCACTTCCA 
     4381 AAATCTGCCT TAGTATTTTG GCGTGCTTTG CTGATGGCAT CCTACCCCTG GACCTGGACG 
     4441 CCAGTGAGTT ACTCTCAGAC ACGTTTGAGG TCCTCAGCTC AAAGGAGATC AAGCTTTTGG 
     4501 CAATGAGATC TAAACCAGAC AAAGACCTCC TTATGGAAGA AGATGACATG GCCTTGGCAA 
     4561 ATGTAGTCAT GCAGGAAGCT CAGAAGAAGC TCATCTCACA AGTTCAGAAG AGGAATTTCA 
     4621 TAGAAAATAT TATTCCAATT ATCATCTCCC TGAAGACTGT GCTGGAGAAA AATAAGATCC 
     4681 CAGCTTTGCG GGAACTCATG CACTATCTCA GGGAGGTGAT GCAGGATTAC CGAGATGAGC 
     4741 TCAAGGACTT CTTTGCAGTT GACAAACAGC TGGCATCAGA GCTTGAGTAT GACATGAAGA 
     4801 AGTACCAGGA ACAGCTGGTC CAGGAGCAGG AGCTAGCAAA ACATGCAGAT GTGGCCGGGA 
     4861 CGGCTGGAGG TGCTGAGGTG GCACCTGTGG CACAGGTTGC CCTGTGTTTA GAAACAGTGC 
     4921 CAGTTCCTGC TGGCCAAGAA AACCCTGCCA TGTCACCTGC CGTGAGCCAG CCCTGCACAC 
     4981 CCAGGGCAAG TGCTGGCCAT GTAGCAGTAT CATCTCCTAC ACCTGAAACA GGGCCATTGC 
     5041 AGAGGTTGCT GCCCAAAGCC AGGCCCATGT CCCTGAGCAC CATTGCAATC CTGAATTCTG 
     5101 TCAAGAAAGC CGTGGAGTCA AAGAGCAGGC ATCGGAGTCG GAGCTTAGGA GTGCTGCCTT 
     5161 TCACTTTAAA TTCTGGAAGC CCAGAAAAAA CGTGCAGTCA GGTGTCTTCA TACAGTTTGG 
     5221 AGCAAGAGTC GAATGGCGAG ATTGAGCACG TGACCAAGCG GGCCATCAGC ACCCCCGAGA 
     5281 AGAGCATCAG TGATGTCACG TTTGGAGCAG GGGTCAGTTA CATCGGGACA CCACGGACTC 
     5341 CGTCGTCAGC CAAAGAGAAA ATTGAAGGCC GGAGTCAAGG AAATGACATC TTATGTTTAT 
     5401 CACTGCCTGA TAAACCGCCC CCACAGCCTC AGCAGTGGAA TGTGCGGTCT CCCGCCAGGA 
     5461 ATAAAGACAC TCCAGCCTGC AGCAGGAGGT CCCTCCGAAA GACCCCTCTG AAAACAGCCA 
     5521 ACACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     5581 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     5641 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5701 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5761 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5821 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5881 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5941 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     6001 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     6061 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     6121 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     6181 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     6241 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     6301 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     6361 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     6421 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     6481 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     6541 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     6601 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6661 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6721 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6781 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6841 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6901 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6961 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     7021 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     7081 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     7141 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     7201 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     7261 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     7321 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     7381 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     7441 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     7501 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     7561 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     7621 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7681 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7741 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7801 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7861 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7921 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7981 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     8041 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     8101 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     8161 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     8221 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     8281 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     8341 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     8401 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     8461 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     8521 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     8581 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     8641 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8701 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8761 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8821 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8881 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8941 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     9001 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     9061 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     9121 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     9181 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     9241 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     9301 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     9361 TTTAACGCGA ATTTT
//