LOCUS       RC210506                7737 bp    DNA     circular  SYN 
DEFINITION  MMRN2 (Myc-DDK-tagged)-Human multimerin 2 (MMRN2)
ACCESSION   NM_024756
VERSION     NM_024756.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3892..3983 
                     /label="Myc-DDK"  
     misc_feature    4013..4598 
                     /label="PolyA signal"  
     misc_feature    4070..4089 
                     /label="Restriction Enzyme"  
     misc_feature    4874..5546 
                     /label="ColE1"  
     misc_feature    5976..6770 
                     /label="Kan/Neo"  
     misc_feature    6805..7107 
                     /label="SV40 ori"  
     misc_feature    7193..7670 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3867..3874 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3867 
                     /label="MMRN2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATCCTGAGC TTGCTGTTCA GCCTTGGGGG CCCCCTGGGC TGGGGGCTGC 
     1081 TGGGGGCATG GGCCCAGGCT TCCAGTACTA GCCTCTCTGA TCTGCAGAGC TCCAGGACAC 
     1141 CTGGGGTCTG GAAGGCAGAG GCTGAGGACA CCGGCAAGGA CCCCGTTGGA CGTAACTGGT 
     1201 GCCCCTACCC AATGTCCAAG CTGGTCACCT TACTAGCTCT TTGCAAAACA GAGAAATTCC 
     1261 TCATCCACTC GCAGCAGCCG TGTCCGCAGG GAGCTCCAGA CTGCCAGAAA GTCAAAGTCA 
     1321 TGTACCGCAT GGCCCACAAG CCAGTGTACC AGGTCAAGCA GAAGGTGCTG ACCTCTTTGG 
     1381 CCTGGAGGTG CTGCCCTGGC TACACGGGCC CCAACTGCGA GCACCACGAT TCCATGGCAA 
     1441 TCCCTGAGCC TGCAGATCCT GGTGACAGCC ACCAGGAACC TCAGGATGGA CCAGTCAGCT 
     1501 TCAAACCTGG CCACCTTGCT GCAGTGATCA ATGAGGTTGA GGTGCAACAG GAACAGCAGG 
     1561 AACATCTGCT GGGAGATCTC CAGAATGATG TGCACCGGGT GGCAGACAGC CTGCCAGGCC 
     1621 TGTGGAAAGC CCTGCCTGGT AACCTCACAG CTGCAGTGAT GGAAGCAAAT CAAACAGGGC 
     1681 ACGAGTTCCC TGATAGATCC TTGGAGCAGG TGCTGCTACC CCACGTGGAC ACCTTCCTAC 
     1741 AAGTGCATTT CAGCCCCATC TGGAGGAGCT TTAACCAAAG CCTGCACAGC CTTACCCAGG 
     1801 CCATAAGAAA CCTGTCTCTT GACGTGGAGG CCAACCGCCA GGCCATCTCC AGAGTCCAGG 
     1861 ACAGTGCCGT GGCCAGGGCT GACTTCCAGG AGCTTGGTGC CAAATTTGAG GCCAAGGTCC 
     1921 AGGAGAACAC TCAGAGAGTG GGTCAGCTGC GACAGGACGT GGAGGACCGC CTGCACGCCC 
     1981 AGCACTTTAC CCTGCACCGC TCGATCTCAG AGCTCCAAGC CGATGTGGAC ACCAAATTGA 
     2041 AGAGGCTGCA CAAGGCTCAG GAGGCCCCAG GGACCAATGG CAGTCTGGTG TTGGCAACGC 
     2101 CTGGGGCTGG GGCAAGGCCT GAGCCGGACA GCCTGCAGGC CAGGCTGGGC CAGCTGCAGA 
     2161 GGAACCTCTC AGAGCTGCAC ATGACCACGG CCCGCAGGGA GGAGGAGTTG CAGTACACCC 
     2221 TGGAGGACAT GAGGGCCACC CTGACCCGGC ACGTGGATGA GATCAAGGAA CTGTACTCCG 
     2281 AATCGGACGA GACTTTCGAT CAGATTAGCA AGGTGGAGCG GCAGGTGGAG GAGCTGCAGG 
     2341 TGAACCACAC GGCGCTCCGT GAGCTGCGCG TGATCCTGAT GGAGAAGTCT CTGATCATGG 
     2401 AGGAGAACAA GGAGGAGGTG GAGCGGCAGC TCCTGGAGCT CAACCTCACG CTGCAGCACC 
     2461 TGCAGGGTGG CCATGCCGAC CTCATCAAGT ACGTGAAGGA CTGCAATTGC CAGAAGCTCT 
     2521 ATTTAGACCT GGACGTCATC CGGGAGGGCC AGAGGGACGC CACGCGTGCC CTGGAGGAGA 
     2581 CCCAGGTGAG CCTGGACGAG CGGCGGCAGC TGGACGGCTC CTCCCTGCAG GCCCTGCAGA 
     2641 ACGCCGTGGA CGCCGTGTCG CTGGCCGTGG ACGCGCACAA AGCGGAGGGC GAGCGGGCGC 
     2701 GGGCGGCCAC GTCGCGGCTC CGGAGCCAAG TGCAGGCGCT GGATGACGAG GTGGGCGCGC 
     2761 TGAAGGCGGC CGCGGCCGAG GCCCGCCACG AGGTGCGCCA GCTGCACAGC GCCTTCGCCG 
     2821 CCCTGCTGGA GGACGCGCTG CGGCACGAGG CGGTGCTGGC CGCGCTCTTC GGGGAGGAGG 
     2881 TGCTGGAGGA GATGTCTGAG CAGACGCCGG GACCGCTGCC CCTGAGCTAC GAGCAGATCC 
     2941 GCGTGGCCCT GCAGGACGCC GCTAGCGGGC TGCAGGAGCA GGCGCTCGGC TGGGACGAGC 
     3001 TGGCCGCCCG AGTGACGGCC CTGGAGCAGG CCTCGGAGCC CCCGCGGCCG GCAGAGCACC 
     3061 TGGAGCCCAG CCACGACGCG GGCCGCGAGG AGGCCGCCAC CACCGCCCTG GCCGGGCTGG 
     3121 CGCGGGAGCT CCAGAGCCTG AGCAACGACG TCAAGAATGT CGGGCGGTGC TGCGAGGCTG 
     3181 AGGCCGGGGC CGGGGCCGCC TCCCTCAACG CCTCCCTTGA CGGCCTCCAC AACGCACTCT 
     3241 TCGCCACTCA GCGCAGCTTG GAGCAGCACC AGCGGCTCTT CCACAGCCTC TTTGGGAACT 
     3301 TCCAAGGGCT CATGGAAGCC AACGTCAGCC TGGACCTGGG GAAGCTGCAG ACCATGCTGA 
     3361 GCAGGAAAGG GAAGAAGCAG CAGAAAGACC TGGAAGCTCC CCGGAAGAGG GACAAGAAGG 
     3421 AAGCGGAGCC TTTGGTGGAC ATACGGGTCA CAGGGCCTGT GCCAGGTGCC TTGGGCGCGG 
     3481 CGCTCTGGGA GGCAGGATCC CCTGTGGCCT TCTATGCCAG CTTTTCAGAA GGGACGGCTG 
     3541 CCCTGCAGAC AGTGAAGTTC AACACCACAT ACATCAACAT TGGCAGCAGC TACTTCCCTG 
     3601 AACATGGCTA CTTCCGAGCC CCTGAGCGTG GTGTCTACCT GTTTGCAGTG AGCGTTGAAT 
     3661 TTGGCCCAGG GCCAGGCACC GGGCAGCTGG TGTTTGGAGG TCACCATCGG ACTCCAGTCT 
     3721 GTACCACTGG GCAGGGGAGT GGAAGCACAG CAACGGTCTT TGCCATGGCT GAGCTGCAGA 
     3781 AGGGTGAGCG AGTATGGTTT GAGTTAACCC AGGGATCAAT AACAAAGAGA AGCCTGTCGG 
     3841 GCACTGCATT TGGGGGCTTC CTGATGTTTA AGACCAGCGG ACCGACGCGT ACGCGGCCGC 
     3901 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3961 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4021 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4081 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4141 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4201 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4261 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4321 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4381 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4441 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4501 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4561 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4621 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4681 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4741 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4801 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4861 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4921 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4981 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5041 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5101 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5161 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5221 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5281 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5341 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5401 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5461 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5521 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5581 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5641 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5701 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5761 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5821 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5881 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5941 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6001 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6061 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6121 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6181 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6241 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6301 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6361 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6421 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6481 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6541 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6601 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6661 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6721 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6781 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6841 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6901 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6961 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7021 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7081 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7141 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7201 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7261 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7321 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7381 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7441 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7501 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7561 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7621 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7681 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//