LOCUS       RC210501                8571 bp    DNA     circular  SYN 
DEFINITION  POLRMT (Myc-DDK-tagged)-Human polymerase (RNA) mitochondrial (DNA directed) (POLRMT), nuclear gene encoding mitochondrial protein
ACCESSION   NM_005035
VERSION     NM_005035.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4728..4819 
                     /label="Myc-DDK"  
     misc_feature    4849..5434 
                     /label="PolyA signal"  
     misc_feature    4906..4925 
                     /label="Restriction Enzyme"  
     misc_feature    5710..6382 
                     /label="ColE1"  
     misc_feature    6812..7606 
                     /label="Kan/Neo"  
     misc_feature    7641..7943 
                     /label="SV40 ori"  
     misc_feature    8029..8506 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4710..4716 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4710 
                     /label="POLRMT"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGGCACTT TGCTGGGGCC GCGGAGCGGC GGGGCTCAAA CGAGCCCTAC 
     1081 GGCCTTGCGG CCGCCCGGGA CTCCCCGGCA AAGAAGGGAC CGCCGGTGGC GTCTGCGGCC 
     1141 CCAGGAGGAG CTCGTCCGCC AGCCCCCAGG AGCAAGACCA AGACCGCAGG AAGGACTGGG 
     1201 GCCACGTGGA GCTGCTGGAG GTGCTCCAGG CGCGGGTGCG GCAGCTGCAG GCTGAGAGCG 
     1261 TGTCGGAGGT GGTGGTGAAC AGGGTGGATG TGGCGCGGCT CCCAGAATGT GGCAGTGGAG 
     1321 ATGGTAGCCT CCAGCCACCC AGGAAGGTCC AGATGGGGGC CAAGGATGCC ACCCCGGTGC 
     1381 CCTGTGGCCG CTGGGCAAAG ATACTGGAGA AGGATAAGCG GACCCAGCAG ATGCGTATGC 
     1441 AGCGGTTGAA GGCGAAGCTG CAGATGCCAT TCCAGAGCGG GGAGTTCAAG GCGCTGACCA 
     1501 GGCGCCTGCA GGTGGAGCCC CGGCTCCTGA GCAAGCAGAT GGCCGGGTGC CTGGAGGACT 
     1561 GCACGCGCCA GGCCCCCGAG AGCCCCTGGG AGGAGCAGCT GGCCCAGCTG CTGCAGGAGG 
     1621 CCCCTGGGAA GCTGAGCCTC GATGTGGAGC AGGCCCCGTC GGGGCAGCAC TCGCAGGCCC 
     1681 AGCTCTCAGG TCAGCAGCAG AGGCTCCTGG CCTTCTTCAA GTGCTGCCTG CTCACTGACC 
     1741 AGCTGCCCCT CGCCCACCAC CTGCTGGTCG TCCACCACGG CCAGCGGCAG AAGCGGAAGC 
     1801 TGCTCACGCT GGACATGTAC AACGCCGTGA TGCTTGGCTG GGCGCGGCAG GGTGCCTTCA 
     1861 AGGAGCTGGT ATATGTGTTA TTCATGGTGA AGGATGCCGG CTTGACCCCG GACCTGCTGT 
     1921 CCTATGCGGC TGCCCTCCAG TGCATGGGGA GGCAGGACCA GGACGCCGGG ACCATCGAAA 
     1981 GGTGTCTGGA ACAGATGAGC CAGGAGGGGC TGAAGCTGCA GGCACTCTTC ACCGCCGTTC 
     2041 TGCTGTCTGA GGAGGATCGG GCCACTGTTC TGAAGGCCGT GCACAAGGTG AAGCCCACCT 
     2101 TCAGCCTCCC GCCGCAGCTG CCGCCCCCGG TCAACACCTC CAAGCTGCTC AGGGACGTGT 
     2161 ATGCCAAGGA TGGGCGTGTG TCCTACCCGA AGCTGCACCT GCCCTTGAAG ACCCTGCAGT 
     2221 GCCTCTTTGA GAAGCAGCTC CACATGGAGC TGGCCAGCAG GGTGTGCGTG GTGTCCGTGG 
     2281 AGAAGCCCAC GTTGCCAAGC AAGGAGGTCA AGCACGCGCG GAAGACCCTG AAGACCCTGC 
     2341 GGGACCAATG GGAGAAAGCA CTGTGCCGGG CGCTGCGGGA GACCAAGAAC CGCCTAGAGC 
     2401 GCGAGGTGTA CGAGGGCCGG TTCTCACTTT ACCCCTTCCT GTGCCTGCTG GACGAGCGCG 
     2461 AGGTGGTGCG GATGCTCCTG CAGGTCCTGC AGGCGCTGCC CGCCCAAGGT GAGTCCTTCA 
     2521 CCACCCTGGC CCGGGAGCTG AGTGCGCGCA CTTTCAGCCG GCACGTGGTG CAGAGGCAGC 
     2581 GGGTCAGTGG CCAGGTGCAG GCGCTGCAGA ACCACTACAG GAAGTACCTC TGCTTGCTGG 
     2641 CCTCCGACGC CGAGGTGCCC GAGCCCTGCC TGCCGCGGCA GTACTGGGAG GCGCTGGGGG 
     2701 CGCCCGAGGC CCTGCGGGAG CAGCCCTGGC CCCTGCCAGT GCAGATGGAG CTGGGCAAGC 
     2761 TGCTGGCGGA GATGCTGGTG CAGGCTACGC AGATGCCATG CAGCCTGGAC AAGCCGCATC 
     2821 ATTCCTCTCG GCTTGTCCCC GTGCTCTACC ACGTGTATTC CTTCCGCAAC GTCCAGCAGA 
     2881 TCGGCATACT GAAGCCGCAC CCGGCCTACG TGCAGCTGCT GGAGAAGGCC GCGGAGCCCA 
     2941 CGCTGACCTT CGAGGCGGTG GATGTACCCA TGCTTTGCCC CCCGCTGCCC TGGACATCGC 
     3001 CGCACTCTGG TGCCTTCCTG CTCAGCCCCA CCAAGCTGAT GCGCACGGTG GAAGGCGCCA 
     3061 CGCAGCACCA GGAGCTGCTG GAAACCTGCC CGCCCACCGC GCTGCATGGC GCACTGGACG 
     3121 CCCTCACCCA ACTGGGCAAC TGCGCCTGGC GCGTCAACGG GCGCGTGCTG GACCTGGTGC 
     3181 TGCAGCTCTT CCAGGCCAAG GGCTGCCCCC AGCTAGGCGT GCCGGCCCCG CCCTCCGAGG 
     3241 CGCCCCAGCC GCCCGAGGCC CACCTGCCGC ACAGCGCCGC GCCCGCCCGC AAGGCCGAGC 
     3301 TGCGCCGTGA GCTGGCGCAC TGCCAGAAGG TGGCCCGGGA GATGCACAGC CTGCGGGCGG 
     3361 AGGCGCTGTA CCGCCTCTCG CTGGCGCAGC ACCTGCGGGA CCGCGTCTTC TGGCTGCCGC 
     3421 ACAACATGGA CTTCCGCGGC CGCACCTACC CCTGCCCGCC GCACTTCAAC CACCTGGGCA 
     3481 GCGACGTGGC GCGGGCCCTG CTGGAGTTCG CCCAGGGCCG CCCGCTCGGC CCGCACGGCC 
     3541 TGGATTGGCT CAAGATCCAC CTGGTCAATC TCACGGGGTT GAAGAAGCGG GAGCCGCTGC 
     3601 GGAAGCGCCT GGCCTTTGCG GAGGAGGTGA TGGATGACAT CCTGGACTCC GCGGACCAAC 
     3661 CCTTGACGGG CCGAAAGTGG TGGATGGGCG CGGAGGAACC CTGGCAGACG CTGGCCTGCT 
     3721 GTATGGAGGT GGCGAACGCT GTGCGCGCCT CCGACCCTGC CGCCTATGTC TCCCACCTCC 
     3781 CCGTCCATCA GGACGGCTCT TGCAACGGCC TGCAGCATTA TGCTGCTCTG GGCCGCGACA 
     3841 GCGTGGGCGC CGCCTCCGTC AACCTGGAGC CCTCGGATGT GCCGCAGGAC GTGTACAGCG 
     3901 GCGTGGCCGC GCAGGTGGAG GTGTTCCGTA GGCAGGACGC CCAGCGGGGC ATGCGGGTGG 
     3961 CACAGGTGCT GGAAGGTTTC ATCACCCGCA AGGTGGTGAA GCAGACGGTG ATGACGGTGG 
     4021 TGTACGGGGT CACGCGCTAT GGCGGGCGCC TGCAGATTGA GAAGCGCCTC CGGGAGCTGA 
     4081 GCGACTTTCC CCAGGAGTTC GTGTGGGAGG CCTCTCACTA TCTCGTACGC CAGGTCTTCA 
     4141 AGAGTCTACA GGAGATGTTC TCGGGGACCC GGGCCATCCA GCACTGGCTG ACCGAGAGTG 
     4201 CCCGCCTCAT CTCCCACATG GGCTCTGTGG TGGAGTGGGT CACACCCCTG GGCGTCCCCG 
     4261 TCATCCAGCC GTATCGCCTG GACTCCAAGG TCAAGCAAAT AGGAGGTGGA ATTCAGAGCA 
     4321 TCACCTACAC CCACAACGGA GACATCAGCC GAAAGCCCAA CACACGTAAG CAGAAGAACG 
     4381 GCTTCCCGCC CAACTTCATC CACTCGCTGG ACTCCTCCCA CATGATGCTC ACCGCCCTGC 
     4441 ACTGCTACAG GAAGGGCCTG ACCTTCGTCT CTGTGCACGA CTGTTACTGG ACTCACGCAG 
     4501 CTGATGTCTC CGTCATGAAC CAGGTGTGCC GGGAGCAGTT TGTCCGCTTG CACAGCGAGC 
     4561 CCATCCTGCA GGACCTGTCC AGATTCCTGG TCAAGCGGTT CTGCTCTGAG CCCCAGAAGA 
     4621 TCTTGGAGGC CAGCCAGCTG AAGGAGACAC TGCAGGCGGT GCCCAAGCCA GGGGCCTTCG 
     4681 ACCTGGAGCA GGTGAAGCGT TCCACCTACT TCTTCAGCAC GCGTACGCGG CCGCTCGAGC 
     4741 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4801 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4861 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4921 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4981 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5041 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5101 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5161 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5221 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5281 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5341 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5401 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5461 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5521 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5581 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5641 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5701 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5761 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5821 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5881 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5941 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6001 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6061 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6121 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6181 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6241 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6301 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6361 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6421 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6481 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6541 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6601 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6661 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6721 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6781 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6841 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6901 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6961 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7021 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7081 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7141 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7201 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7261 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7321 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7381 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7441 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7501 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7561 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7621 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7681 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7741 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7801 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7861 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7921 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7981 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8041 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8101 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8161 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8221 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8281 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8341 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8401 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8461 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8521 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//