LOCUS       RC210431                6630 bp    DNA     circular  SYN 
DEFINITION  STS (Myc-DDK-tagged)-Human steroid sulfatase (microsomal), isozyme S (STS)
ACCESSION   NM_000351
VERSION     NM_000351.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2787..2878 
                     /label="Myc-DDK"  
     misc_feature    2908..3493 
                     /label="PolyA signal"  
     misc_feature    2965..2984 
                     /label="Restriction Enzyme"  
     misc_feature    3769..4441 
                     /label="ColE1"  
     misc_feature    4871..5665 
                     /label="Kan/Neo"  
     misc_feature    5700..6002 
                     /label="SV40 ori"  
     misc_feature    6088..6565 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2769..2775 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2769 
                     /label="STS"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTTTAAGG AAGATGAAGA TCCCTTTCCT CCTACTGTTC TTTCTGTGGG 
     1081 AAGCCGAGAG CCACGCAGCA TCAAGGCCGA ACATCATCCT GGTGATGGCT GACGACCTCG 
     1141 GCATTGGAGA TCCTGGGTGC TATGGGAACA AAACTATCAG GACTCCCAAT ATCGACCGGT 
     1201 TGGCCAGTGG GGGAGTGAAA CTCACTCAGC ACCTGGCAGC ATCACCGCTG TGCACACCAA 
     1261 GCAGGGCAGC CTTCATGACT GGCCGGTACC CTGTCCGATC AGGAATGGCA TCTTGGTCCC 
     1321 GCACTGGAGT TTTCCTCTTC ACAGCCTCTT CGGGAGGACT TCCCACCGAT GAGATTACCT 
     1381 TTGCTAAGCT TCTGAAGGAT CAAGGTTATT CAACAGCACT GATAGGGAAA TGGCACCTTG 
     1441 GGATGAGCTG TCACAGCAAG ACTGACTTCT GTCACCACCC TTTACATCAC GGCTTCAATT 
     1501 ATTTCTATGG GATCTCTTTG ACCAATCTGA GAGACTGCAA GCCCGGAGAG GGCAGTGTCT 
     1561 TCACCACGGG CTTCAAGAGG CTGGTCTTCC TCCCCCTGCA GATCGTCGGG GTCACCCTCC 
     1621 TTACCCTTGC TGCACTCAAT TGTCTGGGGC TACTCCACGT GCCTCTAGGC GTTTTTTTCA 
     1681 GCCTTCTCTT CCTAGCAGCC CTAATCCTGA CCCTTTTCTT GGGCTTCCTT CATTACTTCC 
     1741 GGCCCCTGAA CTGCTTCATG ATGAGGAACT ACGAGATCAT TCAGCAGCCC ATGTCCTATG 
     1801 ACAATCTCAC CCAGAGGCTA ACGGTGGAGG CGGCCCAGTT CATACAGCGG AACACTGAGA 
     1861 CTCCGTTCCT GCTTGTCTTG TCCTACCTCC ACGTGCACAC AGCCCTGTTC TCCAGCAAAG 
     1921 ACTTTGCTGG CAAAAGTCAA CACGGAGTCT ACGGGGATGC TGTTGAGGAA ATGGACTGGA 
     1981 GTGTGGGGCA GATCTTGAAC CTTCTGGATG AGCTGAGATT GGCTAATGAT ACCCTCATCT 
     2041 ACTTCACATC GGACCAGGGA GCACATGTAG AAGAAGTGTC TTCCAAAGGA GAAATTCATG 
     2101 GCGGAAGTAA TGGGATCTAT AAAGGAGGAA AAGCAAACAA CTGGGAAGGA GGTATCCGGG 
     2161 TTCCAGGCAT CCTTCGTTGG CCCAGGGTGA TACAGGCTGG CCAGAAGATT GATGAGCCCA 
     2221 CTAGCAACAT GGACATATTT CCTACAGTAG CCAAGCTGGC TGGAGCTCCC TTGCCTGAGG 
     2281 ACAGGATCAT TGATGGACGT GATCTGATGC CCCTGCTTGA AGGAAAAAGC CAACGCTCCG 
     2341 ATCATGAGTT TCTCTTCCAT TACTGCAACG CCTACTTAAA TGCTGTGCGC TGGCACCCTC 
     2401 AGAACAGCAC ATCCATCTGG AAGGCCTTTT TCTTCACCCC CAACTTCAAC CCCGTGGGTT 
     2461 CCAACGGATG CTTTGCCACA CACGTGTGCT TCTGTTTCGG GAGTTATGTC ACCCATCACG 
     2521 ACCCACCTTT ACTCTTTGAT ATTTCCAAAG ATCCCAGAGA GAGAAACCCA CTAACTCCAG 
     2581 CATCCGAGCC CCGGTTTTAT GAAATCCTCA AAGTCATGCA GGAAGCTGCG GACAGACACA 
     2641 CCCAGACCCT GCCAGAGGTG CCCGATCAGT TTTCATGGAA CAACTTTCTT TGGAAGCCCT 
     2701 GGCTTCAGCT GTGCTGTCCT TCCACCGGCC TGTCTTGCCA GTGTGATAGA GAAAAACAGG 
     2761 ATAAGAGACT GAGCCGCACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     2821 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     2881 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     2941 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     3001 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     3061 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     3121 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     3181 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     3241 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     3301 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     3361 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     3421 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     3481 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     3541 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     3601 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     3661 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     3721 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     3781 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     3841 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     3901 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     3961 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     4021 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     4081 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     4141 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     4201 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     4261 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     4321 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     4381 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     4441 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     4501 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     4561 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     4621 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     4681 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     4741 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     4801 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     4861 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     4921 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     4981 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     5041 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     5101 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     5161 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     5221 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     5281 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     5341 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     5401 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     5461 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     5521 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     5581 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     5641 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     5701 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     5761 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     5821 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     5881 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     5941 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6001 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     6061 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     6121 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     6181 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     6241 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     6301 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     6361 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     6421 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     6481 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     6541 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     6601 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//