LOCUS       RC210392L4                9245 bp    DNA     circular  SYN 
DEFINITION  KCNA10 (Myc-DDK-tagged)-Human potassium voltage-gated channel, shaker-related subfamily, member 10 (KCNA10)
ACCESSION   NM_005549
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7536..7716 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8444..9090 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    6411..6431 
                     /label="LR50"  
     misc_feature    4874..5602 
                     /label="mGFP"  
     misc_feature    5609..5674 
                     /label="P2A"  
     misc_feature    7764..8383 
                     /label="pBR322 Ori"  
     misc_feature    5675..6274 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    4853..4859 
                     /label="Restriction Enzyme"  
     misc_feature    3320..4853 
                     /label="KCNA10"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGATGTGTGT GGCTGGAAAG AAATGGAGGT 
     3361 TGCGCTGGTC AATTTTGATA ATTCAGATGA AATCCAAGAA GAGCCAGGCT ATGCCACAGA 
     3421 CTTCGACTCA ACCAGCCCAA AAGGCCGGCC TGGGGGCAGC TCCTTCTCCA ACGGGAAGAT 
     3481 CCTCATCAGC GAAAGCACCA ACCATGAGAC GGCCTTCTCC AAGCTTCCGG GAGACTATGC 
     3541 TGACCCCCCA GGGCCTGAGC CAGTGGTCCT AAATGAAGGA AACCAGCGGG TGATCATCAA 
     3601 CATTGCTGGG CTGAGATTTG AGACCCAGCT CAGAACCCTT AGTCAGTTCC CAGAGACTCT 
     3661 CCTGGGAGAC CGGGAGAAAA GGATGCAGTT CTTTGACTCC ATGAGAAATG AGTATTTCTT 
     3721 TGATCGTAAC CGGCCCAGTT TTGATGGAAT CCTATATTAT TACCAATCTG GTGGGAAAAT 
     3781 TCGGCGCCCA GCCAATGTTC CCATTGATAT CTTTGCTGAT GAAATCTCCT TCTATGAGCT 
     3841 GGGTAGTGAG GCCATGGACC AGTTCCGGGA GGATGAAGGC TTCATCAAAG ACCCTGAAAC 
     3901 ACTGCTACCC ACCAATGACA TCCACCGTCA GTTCTGGCTC CTCTTTGAGT ACCCTGAAAG 
     3961 TTCCAGCGCT GCCCGTGCTG TGGCCGTGGT CTCGGTGTTG GTTGTGGTCA TCTCCATCAC 
     4021 CATCTTCTGC CTGGAGACAC TGCCAGAGTT CCGGGAGGAT AGGGAGCTGA AGGTGGTCAG 
     4081 AGACCCCAAT CTCAACATGA GCAAGACAGT CCTCTCCCAG ACCATGTTCA CCGACCCTTT 
     4141 CTTCATGGTG GAGTCTACCT GCATCGTGTG GTTCACCTTC GAGCTGGTGC TCCGGTTCGT 
     4201 GGTCTGCCCC AGCAAGACTG ACTTCTTCAG GAACATCATG AACATCATTG ACATCATCTC 
     4261 CATTATCCCC TACTTTGCAA CTCTCATCAC AGAGCTAGTC CAGGAGACAG AGCCGAGTGC 
     4321 CCAACAGAAC ATGTCCCTGG CCATCCTGAG GATCATCCGC CTGGTGAGGG TCTTCCGCAT 
     4381 CTTCAAGCTC TCGCGCCACT CCAAGGGGCT GCAGATCCTC GGGCAAACAC TGAAGGCGTC 
     4441 CATGCGGGAG TTGGGGTTGC TCATCTTCTT TCTCTTCATT GGAGTCATCC TCTTCTCCAG 
     4501 TGCAGTCTAC TTTGCTGAGG TGGATGAGCC AGAGTCCCAT TTCTCTAGCA TTCCTGATGG 
     4561 CTTCTGGTGG GCAGTGGTCA CCATGACAAC TGTAGGCTAT GGGGACATGT GCCCGACCAC 
     4621 CCCAGGGGGG AAGATTGTGG GCACTCTGTG TGCCATTGCA GGGGTCCTCA CCATTGCCCT 
     4681 CCCTGTGCCT GTCATTGTCT CCAACTTCAA TTACTTCTAC CACCGGGAGA CTGAGAATGA 
     4741 AGAAAAGCAG AACATCCCGG GAGAAATTGA AAGAATCCTC AACAGTGTAG GCTCAAGAAT 
     4801 GGGCAGCACA GACTCTCTTA ATAAGACCAA TGGTGGCTGT TCCACAGAGA AGTCTAGGAA 
     4861 AACGCGTACG CGGCCGCTCG AGATGAGCGG GGGCGAGGAG CTGTTCGCCG GCATCGTGCC 
     4921 CGTGCTGATC GAGCTGGACG GCGACGTGCA CGGCCACAAG TTCAGCGTGC GCGGCGAGGG 
     4981 CGAGGGCGAC GCCGACTACG GCAAGCTGGA GATCAAGTTC ATCTGCACCA CCGGCAAGCT 
     5041 GCCCGTGCCC TGGCCCACCC TGGTGACCAC CCTCTGCTAC GGCATCCAGT GCTTCGCCCG 
     5101 CTACCCCGAG CACATGAAGA TGAACGACTT CTTCAAGAGC GCCATGCCCG AGGGCTACAT 
     5161 CCAGGAGCGC ACCATCCAGT TCCAGGACGA CGGCAAGTAC AAGACCCGCG GCGAGGTGAA 
     5221 GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCAAGGACT TCAAGGAGGA 
     5281 CGGCAACATC CTGGGCCACA AGCTGGAGTA CAGCTTCAAC AGCCACAACG TGTACATCCG 
     5341 CCCCGACAAG GCCAACAACG GCCTGGAGGC TAACTTCAAG ACCCGCCACA ACATCGAGGG 
     5401 CGGCGGCGTG CAGCTGGCCG ACCACTACCA GACCAACGTG CCCCTGGGCG ACGGCCCCGT 
     5461 GCTGATCCCC ATCAACCACT ACCTGAGCAC TCAGACCAAG ATCAGCAAGG ACCGCAACGA 
     5521 GGCCCGCGAC CACATGGTGC TCCTGGAGTC CTTCAGCGCC TGCTGCCACA CCCACGGCAT 
     5581 GGACGAGCTG TACAGGTCCG GACTCAGAGT TTGGGTAGGA AGCGGAGCTA CTAACTTCAG 
     5641 CCTGCTGAAG CAGGCTGGAG ACGTGGAGGA GAACCCTGGA CCTATGACCG AGTACAAGCC 
     5701 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC 
     5761 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GATCGCCACA TCGAGCGGGT 
     5821 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT 
     5881 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC 
     5941 GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA 
     6001 GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC 
     6061 CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC 
     6121 CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG 
     6181 CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA 
     6241 AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGATGTACAC CCAAACGGCC 
     6301 GGCCGCGGTC TGTACAAGTA GGATTCGTCG AGGGACCTAA TAACTTCGTA TAGCATACAT 
     6361 TATACGAAGT TATACATGTT TAAGGGTTCC GGTTCCACTA GGTACAATTC GATATCAAGC 
     6421 TTATCGATAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT 
     6481 ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG 
     6541 CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG 
     6601 AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA 
     6661 CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC 
     6721 CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG 
     6781 CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAATCATCG TCCTTTCCTT 
     6841 GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT 
     6901 CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC 
     6961 CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCATC 
     7021 GATACCGTCG ACCTCGATCG AGACCTAGAA AAACATGGAG CAATCACAAG TAGCAATACA 
     7081 GCAGCTACCA ATGCTGATTG TGCCTGGCTA GAAGCACAAG AGGAGGAGGA GGTGGGTTTT 
     7141 CCAGTCACAC CTCAGGTACC TTTAAGACCA ATGACTTACA AGGCAGCTGT AGATCTTAGC 
     7201 CACTTTTTAA AAGAAAAGGG GGGACTGGAA GGGCTAATTC ACTCCCAACG AAGACAAGAT 
     7261 ATCCTTGATC TGTGGATCTA CCACACACAA GGCTACTTCC CTGATTGGCA GAACTACACA 
     7321 CCAGGGCCAG GGATCAGATA TCCACTGACC TTTGGATGGT GCTACAAGCT AGTACCAGTT 
     7381 GAGCAAGAGA AGGTAGAAGA AGCCAATGAA GGAGAGAACA CCCGCTTGTT ACACCCTGTG 
     7441 AGCCTGCATG GGATGGATGA CCCGGAGAGA GAAGTATTAG AGTGGAGGTT TGACAGCCGC 
     7501 CTAGCATTTC ATCACATGGC CCGAGAGCTG CATCCGGACT GTACTGGGTC TCTCTGGTTA 
     7561 GACCAGATCT GAGCCTGGGA GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA 
     7621 TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC 
     7681 TAGAGATCCC TCAGACCCTT TTAGTCAGTG TGGAAAATCT CTAGCAGCAT GTGAGCAAAA 
     7741 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC 
     7801 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA 
     7861 GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG 
     7921 ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT 
     7981 CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT 
     8041 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG 
     8101 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC 
     8161 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC 
     8221 ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA 
     8281 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC 
     8341 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG 
     8401 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GATTACGCCC 
     8461 CGCCCTGCCA CTCATCGCAG TACTGTTGTA ATTCATTAAG CATTCTGCCG ACATGGAAGC 
     8521 CATCACAAAC GGCATGATGA ACCTGAATCG CCAGCGGCAT CAGCACCTTG TCGCCTTGCG 
     8581 TATAATATTT GCCCATGGTG AAAACGGGGG CGAAGAAGTT GTCCATATTG GCCACGTTTA 
     8641 AATCAAAACT GGTGAAACTC ACCCAGGGAT TGGCTGAGAC GAAAAACATA TTCTCAATAA 
     8701 ACCCTTTAGG GAAATAGGCC AGGTTTTCAC CGTAACACGC CACATCTTGC GAATATATGT 
     8761 GTAGAAACTG CCGGAAATCG TCGTGGTATT CACTCCAGAG CGATGAAAAC GTTTCAGTTT 
     8821 GCTCATGGAA AACGGTGTAA CAAGGGTGAA CACTATCCCA TATCACCAGC TCACCGTCTT 
     8881 TCATTGCCAT ACGGAACTCC GGATGAGCAT TCATCAGGCG GGCAAGAATG TGAATAAAGG 
     8941 CCGGATAAAA CTTGTGCTTA TTTTTCTTTA CGGTCTTTAA AAAGGCCGTA ATATCCAGCT 
     9001 GAACGGTCTG GTTATAGGTA CATTGAGCAA CTGACTGAAA TGCCTCAAAA TGTTCTTTAC 
     9061 GATGCCATTG GGATATATCA ACGGTGGTAT ATCCAGTGAT TTTTTTCTCC ATACTCTTCC 
     9121 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG 
     9181 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 
     9241 CTGAC
//