LOCUS       RC210308                8955 bp    DNA     circular  SYN 
DEFINITION  PIK3R4 (Myc-DDK-tagged)-Human phosphoinositide-3-kinase, regulatory subunit 4 (PIK3R4)
ACCESSION   NM_014602
VERSION     NM_014602.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5112..5203 
                     /label="Myc-DDK"  
     misc_feature    5233..5818 
                     /label="PolyA signal"  
     misc_feature    5290..5309 
                     /label="Restriction Enzyme"  
     misc_feature    6094..6766 
                     /label="ColE1"  
     misc_feature    7196..7990 
                     /label="Kan/Neo"  
     misc_feature    8025..8327 
                     /label="SV40 ori"  
     misc_feature    8413..8890 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5094..5100 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5094 
                     /label="PIK3R4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGAAATCAG CTTGCTGGCA TTGCTCCCTC CCAGATCCTT TCTGTAGAGA 
     1081 GTTATTTTTC AGATATTCAT GACTTTGAAT ATGATAAAAG CCTGGGGAGT ACTCGGTTTT 
     1141 TTAAAGTTGC TCGAGCCAAG CACCGAGAAG GCCTGGTCGT TGTGAAGGTT TTTGCAATTC 
     1201 AGGATCCCAC ATTGCCTTTA ACCAGCTATA AACAAGAGCT GGAGGAACTG AAAATCAGGC 
     1261 TTAATTCTGC ACAGAATTGT CTACCTTTCC AGAAAGCATC AGAAAAAGCA TCTGAGAAAG 
     1321 CAGCTATGCT CTTTAGGCAG TATGTGCGAG ACAATCTCTA TGATCGCATC AGTACCCGTC 
     1381 CATTCTTGAA TAACATTGAG AAGCGCTGGA TTGCTTTCCA GATCCTGACA GCTGTGGACC 
     1441 AAGCACACAA ATCTGGAGTT CGTCATGGGG ACATCAAGAC TGAGAATGTG ATGGTCACCA 
     1501 GTTGGAATTG GGTTCTTCTA ACTGATTTTG CCAGTTTTAA GCCCACTTAT CTTCCAGAAG 
     1561 ACAACCCGGC AGATTTCAAT TATTTCTTTG ACACATCACG GAGGAGAACT TGCTATATTG 
     1621 CTCCTGAACG TTTTGTTGAT GGTGGGATGT TTGCCACTGA GTTAGAATAT ATGAGAGATC 
     1681 CTTCAACTCC GCTTGTAGAC TTAAATAGCA ATCAGAGAAC AAGAGGAGAG TTGAAGAGAG 
     1741 CAATGGACAT CTTTTCAGCA GGTTGTGTGA TAGCTGAGCT TTTTACAGAA GGTGTACCAT 
     1801 TATTTGATCT CTCTCAACTT TTGGCTTATA GAAATGGACA TTTTTTCCCT GAACAAGTGC 
     1861 TAAATAAAAT TGAAGATCAC AGTATCAGAG AATTGGTAAC TCAGATGATT CACCGTGAGC 
     1921 CAGATAAACG TTTAGAGGCA GAAGATTACT TAAAACAGCA GCGTGGCAAT GCCTTTCCTG 
     1981 AAATATTTTA CACTTTTCTT CAGCCCTACA TGGCCCAGTT TGCCAAGGAA ACGTTTCTTT 
     2041 CTGCAGATGA GCGTATTCTG GTTATACGGA AGGATTTGGG CAACATTATT CACAATCTCT 
     2101 GTGGACATGA TCTGCCAGAA AAAGCCGAAG GAGAGCCTAA GGAAAATGGG CTGGTTATCT 
     2161 TGGTATCTGT TATAACATCC TGCCTACAGA CCCTTAAATA CTGTGATTCC AAACTAGCTG 
     2221 CTTTGGAACT GATTCTTCAT TTGGCTCCAA GATTAAGTGT TGAAATCCTT TTGGATCGTA 
     2281 TTACTCCATA TCTTTTGCAT TTCAGCAATG ACTCTGTTCC TAGGGTGAGG GCTGAAGCCT 
     2341 TGAGGACGTT GACCAAAGTT CTTGCTCTCG TCAAAGAGGT TCCTCGTAAT GATATCAATA 
     2401 TTTATCCGGA ATACATTCTG CCAGGCATAG CCCACTTAGC CCAAGATGAT GCTACTATCG 
     2461 TTAGACTAGC CTATGCTGAA AACATAGCTC TGCTGGCAGA AACAGCTCTG AGATTCCTGG 
     2521 AATTAGTACA GTTAAAAAAT CTTAATATGG AAAATGACCC CAATAATGAA GAAATAGATG 
     2581 AGGTTACACA TCCAAATGGA AATTATGACA CAGAGCTCCA AGCCTTACAT GAAATGGTCC 
     2641 AGCAGAAAGT TGTTACTTTG CTAAGTGACC CTGAAAATAT TGTAAAACAA ACCTTGATGG 
     2701 AAAATGGAAT AACACGGCTG TGTGTATTCT TTGGACGTCA GAAAGCCAAC GATGTTTTGT 
     2761 TGTCCCACAT GATTACTTTC CTAAATGATA AGAATGATTG GCATCTACGT GGAGCATTTT 
     2821 TTGATAGTAT AGTTGGTGTT GCTGCCTATG TTGGCTGGCA AAGCTCCTCA ATTCTCAAGC 
     2881 CTCTGCTGCA ACAAGGTCTT AGTGATGCTG AGGAATTTGT CATTGTGAAA GCTCTTTATG 
     2941 CCCTTACTTG TATGTGCCAG TTAGGACTGC TACAAAAACC CCATGTTTAC GAATTTGCCA 
     3001 GTGATATTGC CCCCTTCCTG TGTCATCCCA ATTTATGGAT ACGTTATGGT GCCGTGGGAT 
     3061 TTATCACAGT GGTAGCTCGT CAAATAAGTA CAGCTGATGT CTACTGTAAA CTGATGCCTT 
     3121 ATCTTGACCC ATATATTACC CAACCAATAA TACAGATTGA AAGAAAACTT GTTCTGCTCA 
     3181 GTGTTTTAAA GGAACCAGTA AGTCGTTCTA TATTTGATTA TGCTTTGAGG TCTAAAGATA 
     3241 TTACTAGCTT GTTCAGACAT CTTCACATGC GTCAGAAGAA ACGAAATGGT TCTCTTCCCG 
     3301 ACTGCCCTCC GCCAGAGGAT CCTGCCATAG CACAGCTTCT GAAGAAGTTG CTCTCACAGG 
     3361 GAATGACAGA GGAAGAGGAA GACAAACTTC TGGCACTGAA AGACTTCATG ATGAAATCTA 
     3421 ATAAAGCAAA GGCCAATATA GTGGACCAGA GCCATCTTCA TGATAGTAGT CAGAAAGGTG 
     3481 TAATTGACTT GGCAGCTTTA GGCATAACTG GGAGACAAGT TGATCTTGTT AAAACCAAAC 
     3541 AAGAACCAGA TGACAAACGG GCCAGAAAAC ATGTAAAACA AGACTCAAAT GTAAATGAAG 
     3601 AATGGAAAAG CATGTTTGGG TCACTGGACC CACCAAACAT GCCACAGGCC CTACCTAAAG 
     3661 GGAGTGATCA GGAGGTGATT CAGACTGGGA AACCTCCTCG TTCCGAGTCC TCTGCTGGCA 
     3721 TTTGTGTCCC TTTGTCAACT TCTTCACAGG TTCCAGAAGT GACAACTGTC CAAAATAAAA 
     3781 AACCAGTAAT ACCGGTTTTA AGTAGTACAA TCTTACCATC CACCTATCAG ATTCGAATTA 
     3841 CAACTTGTAA AACTGAACTT CAGCAACTCA TCCAGCAAAA GCGGGAGCAG TGCAATGCTG 
     3901 AGAGAATAGC TAAGCAGATG ATGGAAAATG CTGAATGGGA GAGTAAACCA CCACCACCTG 
     3961 GATGGCGTCC TAAAGGGCTG TTAGTTGCCC ATCTTCATGA GCATAAATCT GCTGTGAATC 
     4021 GAATTAGAGT CTCTGATGAA CACTCACTTT TTGCAACATG TTCAAATGAT GGCACAGTGA 
     4081 AAATCTGGAA CAGTCAAAAG ATGGAGGGGA AGACCACCAC TACCAGATCT ATTCTTACAT 
     4141 ACAGCCGAAT TGGAGGACGA GTCAAGACGC TCACATTCTG CCAAGGCTCC CACTATTTAG 
     4201 CCATAGCATC TGATAATGGT GCTGTCCAGC TTCTTGGAAT TGAGGCTTCT AAGCTGCCCA 
     4261 AGTCTCCTAA AATCCATCCT CTACAAAGCA GAATTCTAGA TCAGAAGGAG GACGGTTGTG 
     4321 TTGTGGATAT GCATCACTTC AACTCTGGAG CACAGTCTGT TCTTGCCTAT GCCACTGTGA 
     4381 ATGGCTCTCT GGTTGGCTGG GACCTTAGGT CTTCAAGCAA TGCGTGGACT TTAAAGCATG 
     4441 ATTTAAAGTC GGGCCTCATC ACTTCCTTTG CTGTGGACAT CCACCAATGC TGGCTCTGCA 
     4501 TTGGTACAAG CAGTGGTACC ATGGCTTGTT GGGACATGAG GTTCCAGTTG CCAATTTCAA 
     4561 GTCACTGTCA TCCTTCCAGG GCTCGAATCA GACGCCTCTC AATGCACCCT CTGTATCAGT 
     4621 CCTGGGTGAT TGCAGCTGTT CAGGGCAACA ACGAAGTGTC CATGTGGGAC ATGGAGACTG 
     4681 GTGACAGAAG ATTTACTCTC TGGGCCAGCA GTGCACCACC ACTTTCTGAA TTACAGCCTT 
     4741 CTCCTCATAG CGTCCATGGT ATCTACTGTA GTCCTGCAGA TGGAAATCCT ATCCTACTAA 
     4801 CAGCTGGCTC AGATATGAAA ATAAGGTTTT GGGACTTGGC TTACCCAGAA AGGTCCTATG 
     4861 TTGTTGCAGG AAGTACTAGT TCCCCATCTG TGTCCTACTA CAGGAAAATA ATTGAAGGCA 
     4921 CTGAAGTTGT CCAGGAAATT CAGAATAAGC AGAAAGTAGG ACCAAGTGAT GACACCCCTC 
     4981 GAAGGGGCCC AGAGTCCCTG CCCGTGGGAC ATCATGACAT CATCACTGAT GTCGCCACAT 
     5041 TCCAGACCAC ACAGGGCTTC ATCGTAACTG CTTCTAGAGA TGGGATTGTG AAGGTGTGGA 
     5101 AAACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     5161 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     5221 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5281 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5341 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5401 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5461 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5521 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5581 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5641 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5701 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5761 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5821 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5881 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5941 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     6001 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     6061 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     6121 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     6181 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6241 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6301 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6361 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6421 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6481 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6541 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6601 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6661 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6721 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6781 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6841 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6901 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6961 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     7021 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     7081 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     7141 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     7201 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7261 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7321 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7381 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7441 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7501 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7561 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7621 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7681 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7741 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7801 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7861 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7921 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7981 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     8041 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     8101 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     8161 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     8221 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8281 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8341 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8401 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8461 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8521 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8581 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8641 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8701 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8761 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8821 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8881 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8941 TTTAACGCGA ATTTT
//