LOCUS       RC210197                8529 bp    DNA     circular  SYN 
DEFINITION  PLCB1 (Myc-DDK-tagged)-Human phospholipase C, beta 1 (phosphoinositide-specific) (PLCB1), transcript variant 1
ACCESSION   NM_015192
VERSION     NM_015192.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4686..4777 
                     /label="Myc-DDK"  
     misc_feature    4807..5392 
                     /label="PolyA signal"  
     misc_feature    4864..4883 
                     /label="Restriction Enzyme"  
     misc_feature    5668..6340 
                     /label="ColE1"  
     misc_feature    6770..7564 
                     /label="Kan/Neo"  
     misc_feature    7599..7901 
                     /label="SV40 ori"  
     misc_feature    7987..8464 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4668..4674 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4668 
                     /label="PLCB1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGGGGCT CAACCCGGAG TGCACGCCTT GCAACTCAAG CCCGTGTGCG 
     1081 TGTCCGACAG CCTCAAGAAG GGCACCAAAT TCGTCAAGTG GGATGATGAC TCAACTATTG 
     1141 TTACTCCAAT TATTTTGAGG ACTGACCCTC AGGGATTTTT CTTTTACTGG ACAGATCAAA 
     1201 ACAAGGAGAC AGAGCTACTG GATCTCAGCC TTGTCAAAGA TGCCAGATGT GGGAGACACG 
     1261 CCAAAGCTCC CAAGGACCCC AAATTACGTG AACTTTTGGA TGTGGGGAAC ATCGGGCGCC 
     1321 TGGAGCAGCG CATGATCACA GTGGTGTATG GGCCTGACCT CGTGAACATC TCCCATTTGA 
     1381 ATCTCGTGGC TTTCCAAGAA GAAGTGGCCA AGGAATGGAC AAATGAGGTT TTCAGTTTGG 
     1441 CAACAAACCT GCTGGCCCAA AACATGTCCA GGGATGCATT TCTGGAAAAA GCCTATACTA 
     1501 AACTTAAGCT GCAAGTCACT CCAGAAGGGC GTATTCCTCT CAAAAACATA TATCGCTTGT 
     1561 TTTCAGCAGA TCGGAAGCGA GTTGAAACTG CTTTAGAGGC TTGTAGTCTT CCATCTTCAA 
     1621 GGAATGATTC AATACCTCAA GAAGATTTCA CTCCAGAAGT GTACAGAGTT TTCCTCAACA 
     1681 ACCTTTGCCC TCGACCTGAA ATTGATAACA TCTTTTCAGA ATTTGGTGCA AAAAGCAAAC 
     1741 CATATCTTAC CGTTGATCAG ATGATGGATT TTATCAACCT TAAGCAGCGA GATCCTCGGC 
     1801 TTAATGAAAT ACTTTATCCA CCTCTAAAAC AAGAGCAAGT CCAAGTATTG ATTGAGAAGT 
     1861 ATGAACCCAA CAACAGCCTC GCCAGAAAAG GACAAATATC AGTGGATGGG TTCATGCGCT 
     1921 ATCTGAGTGG AGAAGAAAAC GGAGTCGTTT CACCTGAGAA ACTGGATTTG AATGAAGACA 
     1981 TGTCTCAGCC CCTTTCTCAC TATTTCATTA ATTCCTCGCA CAACACCTAC CTCACAGCTG 
     2041 GCCAACTGGC TGGAAACTCC TCTGTTGAGA TGTATCGCCA AGTGCTCCTG TCTGGTTGTC 
     2101 GCTGTGTGGA GCTGGACTGC TGGAAGGGAC GGACTGCAGA AGAGGAACCT GTCATCACCC 
     2161 ATGGCTTCAC CATGACAACT GAAATATCTT TCAAGGAAGT GATAGAAGCA ATTGCGGAGT 
     2221 GTGCATTTAA GACTTCACCT TTTCCAATTC TCCTTTCGTT TGAGAACCAT GTGGATTCCC 
     2281 CAAAGCAGCA AGCCAAGATG GCGGAGTACT GCCGACTGAT CTTTGGGGAT GCCCTTCTCA 
     2341 TGGAGCCCCT GGAAAAATAT CCACTGGAAT CTGGAGTTCC TCTTCCAAGC CCTATGGATT 
     2401 TAATGTATAA AATTTTGGTG AAAAATAAGA AGAAATCACA CAAGTCATCA GAAGGAAGCG 
     2461 GCAAAAAGAA GCTCTCAGAA CAAGCCTCCA ACACCTACAG TGACTCCTCC AGCATGTTCG 
     2521 AGCCCTCATC CCCAGGAGCC GGAGAAGCTG ATACGGAAAG TGACGACGAC GATGATGATG 
     2581 ATGACTGTAA AAAATCTTCA ATGGATGAGG GGACTGCTGG AAGTGAGGCT ATGGCCACAG 
     2641 AAGAAATGTC TAATCTGGTG AACTATATTC AGCCAGTCAA GTTTGAGTCA TTTGAAATTT 
     2701 CAAAAAAAAG AAATAAAAGT TTTGAAATGT CTTCCTTCGT GGAAACCAAA GGACTTGAAC 
     2761 AACTCACCAA GTCTCCAGTG GAATTTGTAG AATATAACAA AATGCAGCTT AGCAGGATAT 
     2821 ATCCAAAAGG AACACGTGTG GATTCATCCA ACTATATGCC TCAGCTCTTC TGGAATGCAG 
     2881 GTTGTCAGAT GGTGGCACTT AATTTCCAGA CAATGGACCT GGCTATGCAA ATAAATATGG 
     2941 GGATGTATGA ATACAACGGG AAGAGTGGCT ACAGATTGAA GCCAGAGTTC ATGAGGAGGC 
     3001 CTGACAAGCA TTTTGATCCA TTTACTGAAG GCATCGTAGA TGGGATAGTG GCAAACACTT 
     3061 TGTCTGTTAA GATTATTTCA GGTCAGTTTC TTTCTGATAA GAAAGTTGGG ACTTACGTGG 
     3121 AAGTAGATAT GTTTGGTTTG CCTGTGGATA CAAGGAGGAA GGCATTTAAG ACCAAAACAT 
     3181 CCCAAGGAAA TGCTGTGAAT CCTGTCTGGG AAGAAGAACC TATTGTGTTC AAAAAGGTGG 
     3241 TTCTTCCTAC TCTGGCCTGT TTGAGAATAG CAGTTTATGA AGAAGGAGGT AAATTCATTG 
     3301 GCCACCGTAT CTTGCCAGTG CAAGCCATTC GGCCAGGCTA TCACTATATC TGTCTAAGGA 
     3361 ATGAAAGGAA CCAGCCTCTG ACGCTGCCTG CTGTCTTTGT CTACATAGAA GTGAAAGACT 
     3421 ATGTGCCAGA CACATATGCA GATGTCATCG AAGCTTTATC AAACCCAATC CGATATGTGA 
     3481 ACCTGATGGA ACAGAGAGCT AAGCAATTGG CTGCTTTGAC ACTGGAAGAT GAAGAAGAAG 
     3541 TAAAGAAAGA GGCTGATCCT GGAGAAACAC CATCAGAGGC TCCAAGTGAA GCGAGAACGA 
     3601 CTCCAGCAGA AAATGGGGTG AATCACACTA CAACCCTGAC ACCCAAGCCA CCCTCCCAGG 
     3661 CTCTCCACAG CCAGCCAGCT CCAGGTTCTG TAAAGGCACC TGCCAAAACA GAAGATCTTA 
     3721 TTCAGAGTGT CTTAACAGAA GTGGAAGCAC AGACCATCGA AGAACTAAAG CAACAGAAAT 
     3781 CGTTTGTGAA ACTTCAAAAG AAACACTACA AAGAAATGAA AGACCTGGTT AAGAGACACC 
     3841 ACAAGAAAAC CACTGACCTT ATCAAAGAAC ACACTACCAA GTATAATGAA ATTCAGAATG 
     3901 ACTACTTGAG AAGGAGAGCC GCTTTGGAAA AGTCCGCCAA AAAGGACAGT AAGAAAAAAT 
     3961 CGGAACCCAG CAGCCCTGAT CATGGTTCAT CAACGATTGA GCAAGACCTC GCTGCCCTGG 
     4021 ATGCTGAAAT GACCCAAAAG TTAATAGACT TGAAGGACAA ACAACAGCAG CAGCTGCTTA 
     4081 ATCTTCGGCA AGAACAGTAT TATAGTGAAA AATACCAGAA GCGAGAACAT ATTAAACTGC 
     4141 TTATTCAAAA GTTGACGGAT GTCGCAGAAG AGTGTCAGAA CAATCAGTTA AAGAAGCTCA 
     4201 AAGAAATCTG TGAGAAAGAA AAGAAAGAAT TAAAGAAGAA AATGGATAAA AAGAGGCAGG 
     4261 AGAAGATAAC AGAAGCTAAA TCCAAAGACA AAAGTCAGAT GGAAGAGGAG AAGACAGAGA 
     4321 TGATCCGGTC ATATATCCAG GAAGTGGTGC AGTATATCAA GAGGCTAGAA GAAGCGCAAA 
     4381 GTAAACGGCA AGAAAAACTC GTAGAGAAAC ACAAGGAAAT ACGTCAGCAG ATCCTGGATG 
     4441 AAAAGCCCAA GCTGCAGGTG GAGCTGGAGC AAGAATACCA AGACAAATTC AAAAGACTGC 
     4501 CCCTCGAGAT TTTGGAATTC GTGCAGGAAG CCATGAAAGG AAAGATCAGT GAAGACAGCA 
     4561 ATCACGGTTC TGCCCCTCTC TCCCTGTCCT CAGACCCTGG AAAAGTGAAC CACAAGACTC 
     4621 CCTCCAGTGA GGAGCTGGGA GGAGACATCC CAGGAAAAGA ATTTGATACT CCTCTGACGC 
     4681 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4741 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4801 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4861 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4921 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4981 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5041 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5101 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5161 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5221 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5281 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5341 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5401 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5461 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5521 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5581 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5641 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5701 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5761 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5821 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5881 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5941 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6001 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6061 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6121 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6181 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6241 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6301 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6361 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6421 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6481 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6541 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6601 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6661 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6721 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6781 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6841 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6901 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6961 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7021 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7081 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7141 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7201 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7261 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7321 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7381 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7441 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7501 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7561 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7621 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7681 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7741 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7801 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7861 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7921 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7981 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8041 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8101 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8161 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8221 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8281 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8341 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8401 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8461 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8521 GCGAATTTT
//