LOCUS       RC210005                9477 bp    DNA     circular  SYN 
DEFINITION  AGL (Myc-DDK-tagged)-Human amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 1
ACCESSION   NM_000642
VERSION     NM_000642.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5634..5725 
                     /label="Myc-DDK"  
     misc_feature    5755..6340 
                     /label="PolyA signal"  
     misc_feature    5812..5831 
                     /label="Restriction Enzyme"  
     misc_feature    6616..7288 
                     /label="ColE1"  
     misc_feature    7718..8512 
                     /label="Kan/Neo"  
     misc_feature    8547..8849 
                     /label="SV40 ori"  
     misc_feature    8935..9412 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5616..5622 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5616 
                     /label="AGL"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGACACAGT AAACAGATTC GAATTTTACT TCTGAACGAA ATGGAGAAAC 
     1081 TGGAAAAGAC CCTCTTCAGA CTTGAACAAG GGTATGAGCT ACAGTTCCGA TTAGGCCCAA 
     1141 CTTTACAGGG AAAAGCAGTT ACCGTGTATA CAAATTACCC ATTTCCTGGA GAAACATTTA 
     1201 ATAGAGAAAA ATTCCGTTCT CTGGATTGGG AAAATCCAAC AGAAAGAGAA GATGATTCTG 
     1261 ATAAATACTG TAAACTTAAT CTGCAACAAT CTGGTTCATT TCAGTATTAT TTCCTTCAAG 
     1321 GAAATGAGAA AAGTGGTGGA GGTTACATAG TTGTGGACCC CATTTTACGT GTTGGTGCTG 
     1381 ATAATCATGT GCTACCCTTG GACTGTGTTA CTCTTCAGAC ATTTTTAGCT AAGTGTTTGG 
     1441 GACCTTTTGA TGAATGGGAA AGCAGACTTA GGGTTGCAAA AGAATCAGGC TACAACATGA 
     1501 TTCATTTTAC CCCATTGCAG ACTCTTGGAC TATCTAGGTC ATGCTACTCC CTTGCCAATC 
     1561 AGTTAGAATT AAATCCTGAC TTTTCAAGAC CTAATAGAAA GTATACCTGG AATGATGTTG 
     1621 GACAGCTAGT GGAAAAATTA AAAAAGGAAT GGAATGTTAT TTGTATTACT GATGTTGTCT 
     1681 ACAATCATAC TGCTGCTAAT AGTAAATGGA TCCAGGAACA TCCAGAATGT GCCTATAATC 
     1741 TTGTGAATTC TCCACACTTA AAACCTGCCT GGGTCTTAGA CAGAGCACTT TGGCGTTTCT 
     1801 CCTGTGATGT TGCAGAAGGG AAATACAAAG AAAAGGGAAT ACCTGCTTTG ATTGAAAATG 
     1861 ATCACCATAT GAATTCCATC CGAAAAATAA TTTGGGAGGA TATTTTTCCA AAGCTTAAAC 
     1921 TCTGGGAATT TTTCCAAGTA GATGTCAACA AAGCGGTTGA GCAATTTAGA AGACTTCTTA 
     1981 CACAAGAAAA TAGGCGAGTA ACCAAGTCTG ATCCAAACCA ACACCTTACG ATTATTCAAG 
     2041 ATCCTGAATA CAGACGGTTT GGCTGTACTG TAGATATGAA CATTGCACTA ACGACTTTCA 
     2101 TACCACATGA CAAGGGGCCA GCAGCAATTG AAGAATGCTG TAATTGGTTT CATAAAAGAA 
     2161 TGGAGGAATT AAATTCAGAG AAGCATCGAC TCATTAACTA TCATCAGGAA CAGGCAGTTA 
     2221 ATTGCCTTTT GGGAAATGTG TTTTATGAAC GACTGGCTGG CCATGGTCCA AAACTAGGAC 
     2281 CTGTCACTAG AAAGCATCCT TTAGTTACCA GGTATTTTAC TTTCCCATTT GAAGAGATAG 
     2341 ACTTCTCCAT GGAAGAATCT ATGATTCATC TGCCAAATAA AGCTTGTTTT CTGATGGCAC 
     2401 ACAATGGATG GGTAATGGGA GATGATCCTC TTCGAAACTT TGCTGAACCG GGTTCAGAAG 
     2461 TTTACCTAAG GAGAGAACTT ATTTGCTGGG GAGACAGTGT TAAATTACGC TATGGGAATA 
     2521 AACCAGAGGA CTGTCCTTAT CTCTGGGCAC ACATGAAAAA ATACACTGAA ATAACTGCAA 
     2581 CTTATTTCCA GGGAGTACGT CTTGATAACT GCCACTCAAC ACCTCTTCAC GTAGCTGAGT 
     2641 ACATGTTGGA TGCTGCTAGG AATTTGCAAC CCAATTTATA TGTAGTAGCT GAACTGTTCA 
     2701 CAGGAAGTGA AGATCTGGAC AATGTCTTTG TTACTAGACT GGGCATTAGT TCCTTAATAA 
     2761 GAGAGGCAAT GAGTGCATAT AATAGTCATG AAGAGGGCAG ATTAGTTTAC CGATATGGAG 
     2821 GAGAACCTGT TGGATCCTTT GTTCAGCCCT GTTTGAGGCC TTTAATGCCA GCTATTGCAC 
     2881 ATGCCCTGTT TATGGATATT ACGCATGATA ATGAGTGTCC TATTGTGCAT AGATCAGCGT 
     2941 ATGATGCTCT TCCAAGTACT ACAATTGTTT CTATGGCATG TTGTGCTAGT GGAAGTACAA 
     3001 GAGGCTATGA TGAATTAGTG CCTCATCAGA TTTCAGTGGT TTCTGAAGAA CGGTTTTACA 
     3061 CTAAGTGGAA TCCTGAAGCA TTGCCTTCAA ACACAGGTGA AGTTAATTTC CAAAGCGGCA 
     3121 TTATTGCAGC CAGGTGTGCT ATCAGTAAAC TTCATCAGGA GCTTGGAGCC AAGGGTTTTA 
     3181 TTCAGGTGTA TGTGGATCAA GTTGATGAAG ACATAGTGGC AGTAACAAGA CACTCACCTA 
     3241 GCATCCATCA GTCTGTTGTG GCTGTATCTA GAACTGCTTT CAGGAATCCC AAGACTTCAT 
     3301 TTTACAGCAA GGAAGTGCCT CAAATGTGCA TCCCTGGCAA AATTGAAGAA GTAGTTCTTG 
     3361 AAGCTAGAAC TATTGAGAGA AACACGAAAC CTTATAGGAA GGATGAGAAT TCAATCAATG 
     3421 GAACACCAGA TATCACAGTA GAAATTAGAG AACATATTCA GCTTAATGAA AGTAAAATTG 
     3481 TTAAACAAGC TGGAGTTGCC ACAAAAGGGC CCAATGAATA TATTCAAGAA ATAGAATTTG 
     3541 AAAACTTGTC TCCAGGAAGT GTTATTATAT TCAGAGTTAG TCTTGATCCA CATGCACAAG 
     3601 TCGCTGTTGG AATTCTTCGA AATCATCTGA CACAATTCAG TCCTCACTTT AAATCTGGCA 
     3661 GCCTAGCTGT TGACAATGCA GATCCTATAT TAAAAATTCC TTTTGCTTCT CTTGCCTCCA 
     3721 GATTAACTTT GGCTGAGCTA AATCAGATCC TTTACCGATG TGAATCAGAA GAAAAGGAAG 
     3781 ATGGTGGAGG GTGCTATGAC ATACCAAACT GGTCAGCCCT TAAATATGCA GGTCTTCAAG 
     3841 GTTTAATGTC TGTATTGGCA GAAATAAGAC CAAAGAATGA CTTGGGGCAT CCTTTTTGTA 
     3901 ATAATTTGAG ATCTGGAGAT TGGATGATTG ACTATGTCAG TAACCGGCTT ATTTCACGAT 
     3961 CAGGAACTAT TGCTGAAGTT GGTAAATGGT TGCAGGCTAT GTTCTTCTAC CTGAAGCAGA 
     4021 TCCCACGTTA CCTTATCCCA TGTTACTTTG ATGCTATATT AATTGGTGCA TATACCACTC 
     4081 TTCTGGATAC AGCATGGAAG CAGATGTCAA GCTTTGTTCA GAATGGTTCA ACCTTTGTGA 
     4141 AACACCTTTC ATTGGGTTCA GTTCAACTGT GTGGAGTAGG AAAATTCCCT TCCCTGCCAA 
     4201 TTCTTTCACC TGCCCTAATG GATGTACCTT ATAGGTTAAA TGAGATCACA AAAGAAAAGG 
     4261 AGCAATGTTG TGTTTCTCTA GCTGCAGGCT TACCTCATTT TTCTTCTGGT ATTTTCCGCT 
     4321 GCTGGGGAAG GGATACTTTT ATTGCACTTA GAGGTATACT GCTGATTACT GGACGCTATG 
     4381 TAGAAGCCAG GAATATTATT TTAGCATTTG CGGGTACCCT GAGGCATGGT CTCATTCCTA 
     4441 ATCTACTGGG TGAAGGAATT TATGCCAGAT ACAATTGTCG GGATGCTGTG TGGTGGTGGC 
     4501 TGCAGTGTAT CCAGGATTAC TGTAAAATGG TTCCAAATGG TCTAGACATT CTCAAGTGCC 
     4561 CAGTTTCCAG AATGTATCCT ACAGATGATT CTGCTCCTTT GCCTGCTGGC ACACTGGATC 
     4621 AGCCATTGTT TGAAGTCATA CAGGAAGCAA TGCAAAAACA CATGCAGGGC ATACAGTTCC 
     4681 GAGAAAGGAA TGCTGGTCCC CAGATAGATC GAAACATGAA GGACGAAGGT TTTAATATAA 
     4741 CTGCAGGAGT TGATGAAGAA ACAGGATTTG TTTATGGAGG AAATCGTTTC AATTGTGGCA 
     4801 CATGGATGGA TAAAATGGGA GAAAGTGACA GAGCTAGAAA CAGAGGAATC CCAGCCACAC 
     4861 CAAGAGATGG GTCTGCTGTG GAAATTGTGG GCCTGAGTAA ATCTGCTGTT CGCTGGTTGC 
     4921 TGGAATTATC CAAAAAAAAT ATTTTCCCTT ATCATGAAGT CACAGTAAAA AGACATGGAA 
     4981 AGGCTATAAA GGTCTCATAT GATGAGTGGA ACAGAAAAAT ACAAGACAAC TTTGAAAAGC 
     5041 TATTTCATGT TTCCGAAGAC CCTTCAGATT TAAATGAAAA GCATCCAAAT CTGGTTCACA 
     5101 AACGTGGCAT ATACAAAGAT AGTTATGGAG CTTCAAGTCC TTGGTGTGAC TATCAGCTCA 
     5161 GGCCTAATTT TACCATAGCA ATGGTTGTGG CCCCTGAGCT CTTTACTACA GAAAAAGCAT 
     5221 GGAAAGCTTT GGAGATTGCA GAAAAAAAAT TGCTTGGTCC CCTTGGCATG AAAACTTTAG 
     5281 ATCCAGATGA TATGGTTTAC TGTGGAATTT ATGACAATGC ATTAGACAAT GACAACTACA 
     5341 ATCTTGCTAA AGGTTTCAAT TATCACCAAG GACCTGAGTG GCTGTGGCCT ATTGGGTATT 
     5401 TTCTTCGTGC AAAATTATAT TTTTCCAGAT TGATGGGCCC GGAGACTACT GCAAAGACTA 
     5461 TAGTTTTGGT TAAAAATGTT CTTTCCCGAC ATTATGTTCA TCTTGAGAGA TCCCCTTGGA 
     5521 AAGGACTTCC AGAACTGACC AATGAGAATG CCCAGTACTG TCCTTTCAGC TGTGAAACAC 
     5581 AAGCCTGGTC AATTGCTACT ATTCTTGAGA CACTTTATGA TTTAACGCGT ACGCGGCCGC 
     5641 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5701 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5761 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5821 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5881 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5941 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     6001 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     6061 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     6121 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     6181 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     6241 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     6301 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     6361 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     6421 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     6481 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     6541 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     6601 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6661 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6721 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6781 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6841 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6901 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6961 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     7021 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     7081 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     7141 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     7201 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     7261 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     7321 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     7381 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     7441 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     7501 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     7561 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     7621 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7681 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7741 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7801 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7861 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7921 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7981 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     8041 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     8101 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     8161 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     8221 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     8281 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     8341 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     8401 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     8461 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     8521 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     8581 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     8641 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8701 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8761 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8821 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8881 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8941 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     9001 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     9061 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     9121 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     9181 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     9241 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     9301 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     9361 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     9421 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//