LOCUS       RC209951L1                7656 bp    DNA     circular  SYN 
DEFINITION  IRF3 (Myc-DDK-tagged)-Human interferon regulatory factor 3 (IRF3), transcript variant 1
ACCESSION   NM_001571
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    5947..6127 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    6855..7514 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    6286..6854 
                     /label="ColE1"  
     misc_feature    4822..4842 
                     /label="LR50 primer"  
     misc_feature    4619..4690 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    4831..5422 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    4601..4607 
                     /label="Restriction Enzyme"  
     misc_feature    3320..4601 
                     /label="IRF3"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGGAACCCCA AAGCCACGGA TCCTGCCCTG 
     3361 GCTGGTGTCG CAGCTGGACC TGGGGCAACT GGAGGGCGTG GCCTGGGTGA ACAAGAGCCG 
     3421 CACGCGCTTC CGCATCCCTT GGAAGCACGG CCTACGGCAG GATGCACAGC AGGAGGATTT 
     3481 CGGAATCTTC CAGGCCTGGG CCGAGGCCAC TGGTGCATAT GTTCCCGGGA GGGATAAGCC 
     3541 AGACCTGCCA ACCTGGAAGA GGAATTTCCG CTCTGCCCTC AACCGCAAAG AAGGGTTGCG 
     3601 TTTAGCAGAG GACCGGAGCA AGGACCCTCA CGACCCACAT AAAATCTACG AGTTTGTGAA 
     3661 CTCAGGAGTT GGGGACTTTT CCCAGCCAGA CACCTCTCCG GACACCAATG GTGGAGGCAG 
     3721 TACTTCTGAT ACCCAGGAAG ACATTCTGGA TGAGTTACTG GGTAACATGG TGTTGGCCCC 
     3781 ACTCCCAGAT CCGGGACCCC CAAGCCTGGC TGTAGCCCCT GAGCCCTGCC CTCAGCCCCT 
     3841 GCGGAGCCCC AGCTTGGACA ATCCCACTCC CTTCCCAAAC CTGGGGCCCT CTGAGAACCC 
     3901 ACTGAAGCGG CTGTTGGTGC CGGGGGAAGA GTGGGAGTTC GAGGTGACAG CCTTCTACCG 
     3961 GGGCCGCCAA GTCTTCCAGC AGACCATCTC CTGCCCGGAG GGCCTGCGGC TGGTGGGGTC 
     4021 CGAAGTGGGA GACAGGACGC TGCCTGGATG GCCAGTCACA CTGCCAGACC CTGGCATGTC 
     4081 CCTGACAGAC AGGGGAGTGA TGAGCTACGT GAGGCATGTG CTGAGCTGCC TGGGTGGGGG 
     4141 ACTGGCTCTC TGGCGGGCCG GGCAGTGGCT CTGGGCCCAG CGGCTGGGGC ACTGCCACAC 
     4201 ATACTGGGCA GTGAGCGAGG AGCTGCTCCC CAACAGCGGG CATGGGCCTG ATGGCGAGGT 
     4261 CCCCAAGGAC AAGGAAGGAG GCGTGTTTGA CCTGGGGCCC TTCATTGTAG ATCTGATTAC 
     4321 CTTCACGGAA GGAAGCGGAC GCTCACCACG CTATGCCCTC TGGTTCTGTG TGGGGGAGTC 
     4381 ATGGCCCCAG GACCAGCCGT GGACCAAGAG GCTCGTGATG GTCAAGGTTG TGCCCACGTG 
     4441 CCTCAGGGCC TTGGTAGAAA TGGCCCGGGT AGGGGGTGCC TCCTCCCTGG AGAATACTGT 
     4501 GGACCTGCAC ATTTCCAACA GCCACCCACT CTCCCTCACC TCCGACCAGT ACAAGGCCTA 
     4561 CCTGCAGGAC TTGGTGGAGG GCATGGATTT CCAGGGCCCT GGGGAGAGCA CGCGTACGCG 
     4621 GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA 
     4681 CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CTGTACAAGT AGGATTCGTC 
     4741 GAGGGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATACATGT TTAAGGGTTC 
     4801 CGGTTCCACT AGGTACAATT CGATATCAAG CTTATCGATA ATCAACCTCT GGATTACAAA 
     4861 ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC 
     4921 GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC 
     4981 TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT 
     5041 GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC 
     5101 TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC 
     5161 GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG 
     5221 GTGTTGTCGG GGAAATCATC GTCCTTTCCT TGGCTGCTCG CCTGTGTTGC CACCTGGATT 
     5281 CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC 
     5341 CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT 
     5401 CGGATCTCCC TTTGGGCCGC CTCCCCGCAT CGATACCGTC GACCTCGATC GAGACCTAGA 
     5461 AAAACATGGA GCAATCACAA GTAGCAATAC AGCAGCTACC AATGCTGATT GTGCCTGGCT 
     5521 AGAAGCACAA GAGGAGGAGG AGGTGGGTTT TCCAGTCACA CCTCAGGTAC CTTTAAGACC 
     5581 AATGACTTAC AAGGCAGCTG TAGATCTTAG CCACTTTTTA AAAGAAAAGG GGGGACTGGA 
     5641 AGGGCTAATT CACTCCCAAC GAAGACAAGA TATCCTTGAT CTGTGGATCT ACCACACACA 
     5701 AGGCTACTTC CCTGATTGGC AGAACTACAC ACCAGGGCCA GGGATCAGAT ATCCACTGAC 
     5761 CTTTGGATGG TGCTACAAGC TAGTACCAGT TGAGCAAGAG AAGGTAGAAG AAGCCAATGA 
     5821 AGGAGAGAAC ACCCGCTTGT TACACCCTGT GAGCCTGCAT GGGATGGATG ACCCGGAGAG 
     5881 AGAAGTATTA GAGTGGAGGT TTGACAGCCG CCTAGCATTT CATCACATGG CCCGAGAGCT 
     5941 GCATCCGGAC TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG AGCTCTCTGG 
     6001 CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT 
     6061 GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT TTTAGTCAGT 
     6121 GTGGAAAATC TCTAGCAGCA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     6181 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     6241 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6301 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6361 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6421 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6481 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6541 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6601 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6661 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6721 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6781 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6841 ACGTTAAGGG ATTTTGGTCA TGATTACGCC CCGCCCTGCC ACTCATCGCA GTACTGTTGT 
     6901 AATTCATTAA GCATTCTGCC GACATGGAAG CCATCACAAA CGGCATGATG AACCTGAATC 
     6961 GCCAGCGGCA TCAGCACCTT GTCGCCTTGC GTATAATATT TGCCCATGGT GAAAACGGGG 
     7021 GCGAAGAAGT TGTCCATATT GGCCACGTTT AAATCAAAAC TGGTGAAACT CACCCAGGGA 
     7081 TTGGCTGAGA CGAAAAACAT ATTCTCAATA AACCCTTTAG GGAAATAGGC CAGGTTTTCA 
     7141 CCGTAACACG CCACATCTTG CGAATATATG TGTAGAAACT GCCGGAAATC GTCGTGGTAT 
     7201 TCACTCCAGA GCGATGAAAA CGTTTCAGTT TGCTCATGGA AAACGGTGTA ACAAGGGTGA 
     7261 ACACTATCCC ATATCACCAG CTCACCGTCT TTCATTGCCA TACGGAACTC CGGATGAGCA 
     7321 TTCATCAGGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT ATTTTTCTTT 
     7381 ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT ACATTGAGCA 
     7441 ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA 
     7501 TATCCAGTGA TTTTTTTCTC CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7561 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7621 GTCCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGAC
//