LOCUS       RC209913                9003 bp    DNA     circular  SYN 
DEFINITION  MAP3K5 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase 5 (MAP3K5)
ACCESSION   NM_005923
VERSION     NM_005923.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5160..5251 
                     /label="Myc-DDK"  
     misc_feature    5281..5866 
                     /label="PolyA signal"  
     misc_feature    5338..5357 
                     /label="Restriction Enzyme"  
     misc_feature    6142..6814 
                     /label="ColE1"  
     misc_feature    7244..8038 
                     /label="Kan/Neo"  
     misc_feature    8073..8375 
                     /label="SV40 ori"  
     misc_feature    8461..8938 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5142..5148 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5142 
                     /label="MAP3K5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCACGGAG GCGGACGAGG GCATCACTTT CTCTGTGCCA CCCTTCGCCC 
     1081 CCTCGGGCTT CTGCACCATC CCCGAGGGCG GCATCTGCAG GAGGGGAGGA GCGGCGGCGG 
     1141 TGGGCGAGGG CGAGGAGCAC CAGCTGCCAC CGCCGCCGCC GGGCAGTTTC TGGAACGTGG 
     1201 AGAGCGCCGC TGCCCCTGGC ATCGGTTGTC CGGCGGCCAC CTCCTCGAGC AGTGCCACCC 
     1261 GAGGCCGGGG CAGCTCTGTT GGCGGGGGCA GCCGACGGAC CACGGTGGCA TATGTGATCA 
     1321 ACGAAGCGAG CCAAGGGCAA CTGGTGGTGG CCGAGAGCGA GGCCCTGCAG AGCTTGCGGG 
     1381 AGGCGTGCGA GACAGTGGGC GCCACCCTGG AAACCCTGCA TTTTGGGAAA CTCGACTTTG 
     1441 GAGAAACCAC CGTGCTGGAC CGCTTTTACA ATGCAGATAT TGCGGTGGTG GAGATGAGCG 
     1501 ATGCCTTCCG GCAGCCGTCC TTGTTTTACC ACCTTGGGGT GAGAGAAAGT TTCAGCATGG 
     1561 CCAACAACAT CATCCTCTAC TGTGATACTA ACTCGGACTC TCTGCAGTCA CTGAAGGAAA 
     1621 TCATTTGCCA GAAGAATACT ATGTGCACTG GGAACTACAC CTTTGTTCCT TACATGATAA 
     1681 CTCCACATAA CAAAGTCTAC TGCTGTGACA GCAGCTTCAT GAAGGGGTTG ACAGAGCTCA 
     1741 TGCAACCGAA CTTCGAGCTG CTTCTTGGAC CCATCTGCTT ACCTCTTGTG GATCGTTTTA 
     1801 TTCAACTTTT GAAGGTGGCA CAAGCAAGTT CTAGCCAGTA CTTCCGGGAA TCTATACTCA 
     1861 ATGACATCAG GAAAGCTCGT AATTTATACA CTGGTAAAGA ATTGGCAGCT GAGTTGGCAA 
     1921 GAATTCGGCA GCGAGTAGAT AATATCGAAG TCTTGACAGC AGATATTGTC ATAAATCTGT 
     1981 TACTTTCCTA CAGAGATATC CAGGACTATG ATTCTATTGT GAAGCTGGTA GAGACTTTAG 
     2041 AAAAACTGCC AACCTTTGAT TTGGCCTCCC ATCACCATGT GAAGTTTCAT TATGCATTTG 
     2101 CACTGAATAG GAGAAATCTC CCTGGTGACA GAGCAAAAGC TCTTGATATT ATGATTCCCA 
     2161 TGGTGCAAAG CGAAGGACAA GTTGCTTCAG ATATGTATTG CCTAGTTGGT CGAATCTACA 
     2221 AAGATATGTT TTTGGACTCT AATTTCACGG ACACTGAAAG CAGAGACCAT GGAGCTTCTT 
     2281 GGTTCAAAAA GGCATTTGAA TCTGAGCCAA CACTACAGTC AGGAATTAAT TATGCGGTCC 
     2341 TCCTCCTGGC AGCTGGACAC CAGTTTGAAT CTTCCTTTGA GCTCCGGAAA GTTGGGGTGA 
     2401 AGCTAAGTAG TCTTCTTGGT AAAAAGGGAA ACTTGGAAAA ACTCCAGAGC TACTGGGAAG 
     2461 TTGGATTTTT TCTGGGGGCC AGCGTCCTAG CCAATGACCA CATGAGAGTC ATTCAAGCAT 
     2521 CTGAAAAGCT TTTTAAACTG AAGACACCAG CATGGTACCT CAAGTCTATT GTAGAGACAA 
     2581 TTTTAATATA TAAGCATTTT GTGAAACTGA CCACAGAACA GCCTGTGGCC AAGCAAGAAC 
     2641 TTGTGGACTT TTGGATGGAT TTCCTGGTCG AGGCCACAAA GACAGATGTT ACTGTGGTTA 
     2701 GGTTTCCAGT ATTAATATTA GAACCAACCA AAATCTATCA ACCTTCTTAT TTGTCTATCA 
     2761 ACAATGAAGT TGAGGAAAAG ACAATCTCTA TTTGGCACGT GCTTCCTGAT GACAAGAAAG 
     2821 GTATACATGA GTGGAATTTT AGTGCCTCTT CTGTCAGGGG AGTGAGTATT TCTAAATTTG 
     2881 AAGAAAGATG CTGCTTTCTT TATGTGCTTC ACAATTCTGA TGATTTCCAA ATCTATTTCT 
     2941 GTACAGAACT TCATTGTAAA AAGTTTTTTG AGATGGTGAA CACCATTACC GAAGAGAAGG 
     3001 GGAGAAGCAC AGAGGAAGGA GACTGTGAAA GTGACTTGCT GGAGTATGAC TATGAATATG 
     3061 ATGAAAATGG TGACAGAGTC GTTTTAGGAA AAGGCACTTA TGGGATAGTC TACGCAGGTC 
     3121 GGGACTTGAG CAACCAAGTC AGAATTGCTA TTAAGGAAAT CCCAGAGAGA GACAGCAGAT 
     3181 ACTCTCAGCC CCTGCATGAA GAAATAGCAT TGCATAAACA CCTGAAGCAC AAAAATATTG 
     3241 TCCAGTATCT GGGCTCTTTC AGTGAGAATG GTTTCATTAA AATCTTCATG GAGCAGGTCC 
     3301 CTGGAGGAAG TCTTTCTGCT CTCCTTCGTT CCAAATGGGG TCCATTAAAA GACAATGAGC 
     3361 AAACAATTGG CTTTTATACA AAGCAAATAC TGGAAGGATT AAAATATCTC CATGACAATC 
     3421 AGATAGTTCA CCGGGACATA AAGGGTGACA ATGTGTTGAT TAATACCTAC AGTGGTGTTC 
     3481 TCAAGATCTC TGACTTCGGA ACATCAAAGA GGCTTGCTGG CATAAACCCC TGTACTGAAA 
     3541 CTTTTACTGG TACCCTCCAG TATATGGCAC CAGAAATAAT AGATAAAGGA CCAAGAGGCT 
     3601 ACGGAAAAGC AGCAGACATC TGGTCTCTGG GCTGTACAAT CATTGAAATG GCCACAGGAA 
     3661 AACCCCCATT TTATGAACTG GGAGAACCAC AAGCAGCTAT GTTCAAGGTG GGAATGTTTA 
     3721 AAGTCCACCC TGAGATCCCA GAGTCCATGT CTGCAGAGGC CAAGGCATTC ATACTGAAAT 
     3781 GTTTTGAACC AGATCCTGAC AAGAGAGCCT GTGCTAACGA CTTGCTTGTT GATGAGTTTT 
     3841 TAAAAGTTTC AAGCAAAAAG AAAAAGACAC AACCTAAGCT TTCAGCTCTT TCAGCTGGAT 
     3901 CAAATGAATA TCTCAGGAGT ATATCCTTGC CGGTACCTGT GCTGGTGGAG GACACCAGCA 
     3961 GCAGCAGTGA GTACGGCTCA GTTTCACCCG ACACGGAGTT GAAAGTGGAC CCCTTCTCTT 
     4021 TCAAAACAAG AGCCAAGTCC TGCGGAGAAA GAGATGTCAA GGGAATTCGG ACACTCTTTT 
     4081 TGGGCATTCC AGATGAGAAT TTTGAAGATC ACAGTGCTCC TCCTTCCCCT GAAGAAAAAG 
     4141 ATTCTGGATT CTTCATGCTG AGGAAGGACA GTGAGAGGCG AGCTACCCTT CACAGGATCC 
     4201 TGACGGAAGA CCAAGACAAA ATTGTGAGAA ACCTAATGGA ATCTTTAGCT CAGGGGGCTG 
     4261 AAGAACCGAA ACTAAAATGG GAACACATCA CAACCCTCAT TGCAAGCCTC AGAGAATTTG 
     4321 TGAGATCCAC TGACCGAAAA ATCATAGCCA CCACACTGTC AAAGCTGAAA CTGGAGCTGG 
     4381 ACTTCGACAG CCATGGCATT AGCCAAGTCC AGGTGGTACT CTTTGGTTTT CAAGATGCTG 
     4441 TCAATAAAGT TCTTCGGAAT CATAACATCA AGCCGCACTG GATGTTTGCC TTAGACAGTA 
     4501 TCATTCGGAA GGCGGTACAG ACAGCCATTA CCATCCTGGT TCCAGAACTA AGGCCACATT 
     4561 TCAGCCTTGC ATCTGAGAGT GATACTGCTG ATCAAGAAGA CTTGGATGTA GAAGATGACC 
     4621 ATGAGGAACA GCCTTCAAAT CAAACTGTCC GAAGACCTCA GGCTGTCATT GAAGATGCTG 
     4681 TGGCTACCTC AGGCGTGAGC ACGCTCAGTT CTACTGTGTC TCATGATTCC CAGAGTGCTC 
     4741 ACCGGTCACT GAATGTACAG CTTGGAAGGA TGAAAATAGA AACCAATAGA TTACTGGAAG 
     4801 AATTGGTTCG GAAAGAGAAA GAATTACAAG CACTCCTTCA TCGAGCTATT GAAGAAAAAG 
     4861 ACCAAGAAAT TAAACACCTG AAGCTTAAGT CCCAACCCAT AGAAATTCCT GAATTGCCTG 
     4921 TATTTCATCT AAATTCTTCT GGCACAAATA CTGAAGATTC TGAACTTACC GACTGGCTGA 
     4981 GAGTGAATGG AGCTGATGAA GACACTATAA GCCGGTTTTT GGCTGAAGAT TATACACTAT 
     5041 TGGATGTTCT CTACTATGTT ACACGTGATG ACTTAAAATG CTTGAGACTA AGGGGAGGGA 
     5101 TGCTGTGCAC ACTGTGGAAG GCTATCATTG ACTTTCGAAA CAAACAGACT ACGCGTACGC 
     5161 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     5221 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     5281 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5341 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5401 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5461 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5521 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5581 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5641 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5701 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5761 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5821 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5881 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5941 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     6001 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     6061 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     6121 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     6181 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     6241 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6301 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6361 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6421 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6481 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6541 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6601 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6661 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6721 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6781 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6841 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6901 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6961 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     7021 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     7081 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     7141 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     7201 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     7261 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7321 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7381 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7441 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7501 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7561 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7621 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7681 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7741 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7801 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7861 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7921 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7981 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     8041 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     8101 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     8161 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     8221 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     8281 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8341 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8401 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8461 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8521 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8581 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8641 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8701 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8761 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8821 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8881 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8941 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     9001 TTT
//