LOCUS       RC209861                8244 bp    DNA     circular  SYN 
DEFINITION  PLEKHA7 (Myc-DDK-tagged)-Human pleckstrin homology domain containing, family A member 7 (PLEKHA7)
ACCESSION   NM_175058
VERSION     NM_175058.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4401..4492 
                     /label="Myc-DDK"  
     misc_feature    4522..5107 
                     /label="PolyA signal"  
     misc_feature    4579..4598 
                     /label="Restriction Enzyme"  
     misc_feature    5383..6055 
                     /label="ColE1"  
     misc_feature    6485..7279 
                     /label="Kan/Neo"  
     misc_feature    7314..7616 
                     /label="SV40 ori"  
     misc_feature    7702..8179 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4383..4389 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4383 
                     /label="PLEKHA7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGGCG ACGGTCGGGC GGGACACTTT ACCTGAGCAT TGGTCCTACG 
     1081 GGGTGTGCCG GGATGGCCGC GTCTTCTTCA TCAATGACCA GCTCCGCTGC ACGACCTGGC 
     1141 TGCATCCGCG CACCGGGGAG CCCGTCAACT CGGGCCACAT GATCCGCTCA GACCTGCCCC 
     1201 GCGGCTGGGA GGAGGGCTTC ACGGAGGAGG GCGCCAGCTA CTTCATCGAC CATAACCAGC 
     1261 AGACCACAGC ATTCAGGCAT CCTGTGACGG GACAGTTTTC TCCAGAAAAT AGTGAATTCA 
     1321 TTCTTCAAGA AGAGCCGAAT CCACATATGT CGAAGCAAGA CAGAAACCAA AGACCGTCCA 
     1381 GCATGGTCAG TGAAACATCC ACGGCTGGGA CCGCCTCCAC CCTGGAGGCC AAGCCTGGAC 
     1441 CCAAGATCAT AAAGTCCAGC AGTAAAGTCC ACAGCTTTGG GAAGAGAGAC CAGGCCATTC 
     1501 GGAGGAACCC CAATGTTCCC GTGGTGGTGA GGGGCTGGCT GCACAAGCAG GACAGTTCTG 
     1561 GGATGAGGCT GTGGAAAAGG AGGTGGTTTG TGCTTGCTGA TTACTGCTTA TTTTACTATA 
     1621 AAGACAGCCG AGAAGAAGCG GTCCTCGGGA GCATCCCCTT GCCCAGCTAC GTGATCTCTC 
     1681 CTGTGGCCCC TGAGGATCGC ATAAGCCGCA AATATTCCTT TAAGGCTGTG CACACGGGGA 
     1741 TGCGAGCGCT CATCTATAAC AGCTCCACAG CGGGCTCTCA GGCCGAGCAG TCAGGCATGA 
     1801 GGACCTACTA CTTCAGTGCC GACACCCAGG AGGACATGAA CGCTTGGGTC AGGGCCATGA 
     1861 ACCAGGCTGC ACAGGTGCTG TCTCGATCGT CACTGAAGAG GGATATGGAG AAGGTGGAGC 
     1921 GGCAGGCTGT CCCCCAGGCC AACCACACAG AGTCCTGTCA CGAATGTGGC CGGGTGGGAC 
     1981 CCGGACATAC GAGAGATTGT CCTCATCGTG GCCATGATGA CATTGTCAAC TTCGAGAGGC 
     2041 AGGAGCAGGA GGGAGAGCAG TACCGTTCCC AGAGGGACCC ACTGGAGGGC AAGCGGGACC 
     2101 GGAGCAAGGC CAGGTCTCCG TACTCGCCAG CCGAGGAGGA TGCCTTGTTT ATGGATTTAC 
     2161 CCACTGGCCC AAGAGGCCAG CAGGCACAGC CCCAACGGGC AGAGAAGAAT GGAATGCTGC 
     2221 CTGCCTCATA TGGCCCAGGA GAACAGAATG GGACTGGTGG GTACCAGCGG GCCTTTCCTC 
     2281 CCAGGACCAA CCCTGAAAAA CACAGCCAAA GGAAGAGCAA TCTGGCCCAG GTGGAGCACT 
     2341 GGGCAAGGGC CCAGAAAGGG GATAGCAGGA GTCTTCCCTT GGACCAGACG CTTCCTCGCC 
     2401 AGGGTCCTGG CCAATCCCTG TCCTTCCCAG AAAACTACCA GACTCTTCCC AAGAGCACCC 
     2461 GACACCCCTC GGGGGGCTCC TCGCCACCTC CCCGAAACCT GCCAAGTGAC TACAAGTATG 
     2521 CGCAGGACCG AGCCAGCCAC CTGAAGATGT CGAGTGAAGA GCGCCGGGCG CACCGGGATG 
     2581 GCACCGTGTG GCAGCTCTAC GAGTGGCAGC AGCGCCAGCA GTTCCGGCAC GGCAGCCCCA 
     2641 CAGCGCCCAT CTGCCTTGGC TCCCCAGAGT TCACCGACCA GGGCCGGAGC AGGAGCATGC 
     2701 TAGAGGTGCC CCGCTCCATC TCTGTGCCTC CATCTCCCTC GGACATCCCT CCCCCAGGAC 
     2761 CCCCAAGGGT CTTCCCACCC CGGCGGCCAC ACACACCAGC AGAGCGAGTC ACAGTGAAGC 
     2821 CACCGGACCA GAGGAGGAGT GTGGACATCT CGCTGGGGGA TTCTCCAAGG AGGGCACGGG 
     2881 GCCACGCTGT CAAGAACTCA TCTCATGTGG ACCGACGTTC CATGCCCTCC ATGGGTTACA 
     2941 TGACCCACAC CGTCAGCGCT CCCAGTTTAC ATGGAAAATC GGCTGATGAT ACCTACCTCC 
     3001 AGCTGAAGAA AGACCTGGAG TACCTGGATC TAAAGATGAC AGGCCGGGAC CTTCTCAAGG 
     3061 ATCGAAGTCT GAAGCCTGTG AAGATCGCTG AGAGCGACAC TGACGTCAAA CTGAGCATCT 
     3121 TCTGTGAACA AGACAGGGTC CTCCAGGACT TGGAAGACAA GATACGAGCC CTTAAAGAGA 
     3181 ACAAAGACCA GCTAGAATCT GTGCTGGAGG TGTTGCACAG ACAGATGGAG CAGTACCGAG 
     3241 ACCAGCCCCA GCACTTGGAG AAGATTGCCT ACCAGCAGAA GTTGCTGCAG GAGGACCTTG 
     3301 TCCATATCCG AGCTGAGCTC TCCAGAGAGT CCACTGAGAT GGAAAATGCT TGGAACGAAT 
     3361 ACCTGAAGTT GGAGAATGAT GTGGAACAGC TGAAGCAGAC CCTGCAGGAG CAACACAGAA 
     3421 GAGCCTTTTT TTTCCAGGAG AAATCGCAGA TACAGAAAGA TCTATGGAGA ATTGAAGATG 
     3481 TCACTGCAGG CCTGAGTGCA AATAAAGAGA ACTTCAGAAT TCTAGTGGAG TCAGTAAAAA 
     3541 ATCCGGAGAG AAAAACGGTG CCTTTGTTTC CTCACCCGCC TGTGCCTTCA CTCTCAACTT 
     3601 CTGAGAGCAA GCCGCCCCCA CAGCCCAGTC CTCCCACCAG CCCTGTGCGG ACCCCTCTGG 
     3661 AGGTTCGACT CTTCCCCCAG CTGCAAACCT ACGTGCCGTA CCGACCTCAC CCACCCCAGC 
     3721 TGAGGAAAGT GACATCCCCC CTTCAGTCAC CAACTAAGGC GAAGCCCAAA GTTGAAGATG 
     3781 AAGCACCTCC CAGGCCCCCA CTCCCCGAAC TCTACAGCCC AGAGGACCAG CCCCCGGCTG 
     3841 TGCCGCCTCT GCCAAGAGAG GCCACCATCA TCCGGCACAC ATCTGTGCGG GGCCTCAAGC 
     3901 GGCAGTCAGA CGAGAGGAAG CGAGACCGGG AGCTGGGGCA GTGTGTGAAT GGGGATTCCA 
     3961 GGGTGGAGCC GCGGTCGTAT GTCAGTGAGC CTGAGCTGGC GACCCTCAGC GGGGACATGG 
     4021 CCCAGCCCTC CCTAGGACTT GTGGGCCCTG AGAGCAGGTA CCAGACGCTG CCAGGCAGAG 
     4081 GGCTCTCAGG GTCCACGTCA AGGCTCCAGC AGTCGTCCAC CATTGCTCCC TACGTCACAC 
     4141 TCCGGAGGGG TCTCAATGCC GAAAGCAGCA AGGCGACCTT CCCTAGACCT AAGAGTGCCT 
     4201 TGGAGCGCCT GTACTCAGGG GATCACCAGC GAGGCAAGAT GAGTGCAGAG GAGCAGCTGG 
     4261 AGCGCATGAA GCGACACCAG AAGGCCCTGG TCCGAGAGCG CAAGAGGACA CTGGGCCAAG 
     4321 GGGAGAGGAC GGGCCTGCCC TCATCTCGCT ACCTCAGCCG GCCGCTCCCT GGAGATCTTG 
     4381 GCTCAGTATG TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4441 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4501 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4561 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4621 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4681 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4741 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4801 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4861 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4921 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4981 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5041 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5101 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5161 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5221 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5281 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5341 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5401 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5461 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5521 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5581 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5641 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5701 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5761 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5821 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5881 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5941 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6001 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6061 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6121 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6181 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6241 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6301 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6361 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6421 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6481 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6541 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6601 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6661 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6721 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6781 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6841 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6901 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6961 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7021 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7081 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7141 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7201 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7261 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7321 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7381 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7441 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7501 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7561 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7621 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7681 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7741 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7801 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7861 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7921 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7981 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8041 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8101 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8161 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8221 TAACAAAAAT TTAACGCGAA TTTT
//