LOCUS       RC209846L2                9054 bp    DNA     circular  SYN 
DEFINITION  FOXO3 (Myc-DDK-tagged)-Human forkhead box O3 (FOXO3), transcript variant 1
ACCESSION   NM_001455
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7345..7525 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8253..8912 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7573..8192 
                     /label="ColE1"  
     misc_feature    6220..6240 
                     /label="LR50"  
     misc_feature    5360..6085 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    6229..6820 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5339..5345 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5339 
                     /label="FOXO3"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCAGAGGCA CCGGCTTCCC CGGCCCCGCT 
     3361 CTCTCCGCTC GAAGTGGAGC TGGACCCGGA GTTCGAGCCC CAGAGCCGTC CGCGATCCTG 
     3421 TACGTGGCCC CTGCAAAGGC CGGAGCTCCA AGCGAGCCCT GCCAAGCCCT CGGGGGAGAC 
     3481 GGCCGCTGAC TCCATGATCC CCGAGGAGGA GGACGATGAA GACGACGAGG ACGGCGGGGG 
     3541 ACGGGCCGGC TCGGCCATGG CGATCGGCGG CGGCGGCGGG AGCGGCACGC TGGGCTCCGG 
     3601 GCTGCTCCTT GAGGACTCGG CCCGGGTGCT GGCACCCGGA GGGCAAGACC CCGGGTCTGG 
     3661 GCCAGCCACC GCGGCGGGCG GGCTGAGCGG GGGTACACAG GCGCTGCTGC AGCCTCAGCA 
     3721 ACCGCTGCCA CCGCCGCAGC CGGGGGCGGC TGGGGGCTCC GGGCAGCCGA GGAAATGTTC 
     3781 GTCGCGGCGG AACGCCTGGG GAAACCTGTC CTACGCGGAC CTGATCACCC GCGCCATCGA 
     3841 GAGCTCCCCG GACAAACGGC TCACTCTGTC CCAGATCTAC GAGTGGATGG TGCGTTGCGT 
     3901 GCCCTACTTC AAGGATAAGG GCGACAGCAA CAGCTCTGCC GGCTGGAAGA ACTCCATCCG 
     3961 GCACAACCTG TCACTGCATA GTCGATTCAT GCGGGTCCAG AATGAGGGAA CTGGCAAGAG 
     4021 CTCTTGGTGG ATCATCAACC CTGATGGGGG GAAGAGCGGA AAAGCCCCCC GGCGGCGGGC 
     4081 TGTCTCCATG GACAATAGCA ACAAGTATAC CAAGAGCCGT GGCCGCGCAG CCAAGAAGAA 
     4141 GGCAGCCCTG CAGACAGCCC CCGAATCAGC TGACGACAGT CCCTCCCAGC TCTCCAAGTG 
     4201 GCCTGGCAGC CCCACGTCAC GCAGCAGTGA TGAGCTGGAT GCGTGGACGG ACTTCCGTTC 
     4261 ACGCACCAAT TCTAACGCCA GCACAGTCAG TGGCCGCCTG TCGCCCATCA TGGCAAGCAC 
     4321 AGAGTTGGAT GAAGTCCAGG ACGATGATGC GCCTCTCTCG CCCATGCTCT ACAGCAGCTC 
     4381 AGCCAGCCTG TCACCTTCAG TAAGCAAGCC GTGCACGGTG GAACTGCCAC GGCTGACTGA 
     4441 TATGGCAGGC ACCATGAATC TGAATGATGG GCTGACTGAA AACCTCATGG ACGACCTGCT 
     4501 GGATAACATC ACGCTCCCGC CATCCCAGCC ATCGCCCACT GGGGGACTCA TGCAGCGGAG 
     4561 CTCTAGCTTC CCGTATACCA CCAAGGGCTC GGGCCTGGGC TCCCCAACCA GCTCCTTTAA 
     4621 CAGCACGGTG TTCGGACCTT CATCTCTGAA CTCCCTACGC CAGTCTCCCA TGCAGACCAT 
     4681 CCAAGAGAAC AAGCCAGCTA CCTTCTCTTC CATGTCACAC TATGGTAACC AGACACTCCA 
     4741 GGACCTGCTC ACTTCGGACT CACTTAGCCA CAGCGATGTC ATGATGACAC AGTCGGACCC 
     4801 CTTGATGTCT CAGGCCAGCA CCGCTGTGTC TGCCCAGAAT TCCCGCCGGA ACGTGATGCT 
     4861 TCGCAATGAT CCGATGATGT CCTTTGCTGC CCAGCCTAAC CAGGGAAGTT TGGTCAATCA 
     4921 GAACTTGCTC CACCACCAGC ACCAAACCCA GGGCGCTCTT GGTGGCAGCC GTGCCTTGTC 
     4981 GAATTCTGTC AGCAACATGG GCTTGAGTGA GTCCAGCAGC CTTGGGTCAG CCAAACACCA 
     5041 GCAGCAGTCT CCTGTCAGCC AGTCTATGCA AACCCTCTCG GACTCTCTCT CAGGCTCCTC 
     5101 CTTGTACTCA ACTAGTGCAA ACCTGCCCGT CATGGGCCAT GAGAAGTTCC CCAGCGACTT 
     5161 GGACCTGGAC ATGTTCAATG GGAGCTTGGA ATGTGACATG GAGTCCATTA TCCGTAGTGA 
     5221 ACTCATGGAT GCTGATGGGT TGGATTTTAA CTTTGATTCC CTCATCTCCA CACAGAATGT 
     5281 TGTTGGTTTG AACGTGGGGA ACTTCACTGG TGCTAAGCAG GCCTCATCTC AGAGCTGGGT 
     5341 GCCAGGCACG CGTACGCGGC CGCTCGAGAT GAGCGGGGGC GAGGAGCTGT TCGCCGGCAT 
     5401 CGTGCCCGTG CTGATCGAGC TGGACGGCGA CGTGCACGGC CACAAGTTCA GCGTGCGCGG 
     5461 CGAGGGCGAG GGCGACGCCG ACTACGGCAA GCTGGAGATC AAGTTCATCT GCACCACCGG 
     5521 CAAGCTGCCC GTGCCCTGGC CCACCCTGGT GACCACCCTC TGCTACGGCA TCCAGTGCTT 
     5581 CGCCCGCTAC CCCGAGCACA TGAAGATGAA CGACTTCTTC AAGAGCGCCA TGCCCGAGGG 
     5641 CTACATCCAG GAGCGCACCA TCCAGTTCCA GGACGACGGC AAGTACAAGA CCCGCGGCGA 
     5701 GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA AGGACTTCAA 
     5761 GGAGGACGGC AACATCCTGG GCCACAAGCT GGAGTACAGC TTCAACAGCC ACAACGTGTA 
     5821 CATCCGCCCC GACAAGGCCA ACAACGGCCT GGAGGCTAAC TTCAAGACCC GCCACAACAT 
     5881 CGAGGGCGGC GGCGTGCAGC TGGCCGACCA CTACCAGACC AACGTGCCCC TGGGCGACGG 
     5941 CCCCGTGCTG ATCCCCATCA ACCACTACCT GAGCACTCAG ACCAAGATCA GCAAGGACCG 
     6001 CAACGAGGCC CGCGACCACA TGGTGCTCCT GGAGTCCTTC AGCGCCTGCT GCCACACCCA 
     6061 CGGCATGGAC GAGCTGTACA GGTCCGGACT CAGATAAGTT TAAACGGCCG GCCGCGGTCT 
     6121 GTACAAGTAG GATTCGTCGA GGGACCTAAT AACTTCGTAT AGCATACATT ATACGAAGTT 
     6181 ATACATGTTT AAGGGTTCCG GTTCCACTAG GTACAATTCG ATATCAAGCT TATCGATAAT 
     6241 CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT 
     6301 TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG 
     6361 GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG 
     6421 CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT 
     6481 TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT 
     6541 GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG 
     6601 GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAATCATCGT CCTTTCCTTG GCTGCTCGCC 
     6661 TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT 
     6721 CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC 
     6781 CTTCGCCCTC AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCATCG ATACCGTCGA 
     6841 CCTCGATCGA GACCTAGAAA AACATGGAGC AATCACAAGT AGCAATACAG CAGCTACCAA 
     6901 TGCTGATTGT GCCTGGCTAG AAGCACAAGA GGAGGAGGAG GTGGGTTTTC CAGTCACACC 
     6961 TCAGGTACCT TTAAGACCAA TGACTTACAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA 
     7021 AGAAAAGGGG GGACTGGAAG GGCTAATTCA CTCCCAACGA AGACAAGATA TCCTTGATCT 
     7081 GTGGATCTAC CACACACAAG GCTACTTCCC TGATTGGCAG AACTACACAC CAGGGCCAGG 
     7141 GATCAGATAT CCACTGACCT TTGGATGGTG CTACAAGCTA GTACCAGTTG AGCAAGAGAA 
     7201 GGTAGAAGAA GCCAATGAAG GAGAGAACAC CCGCTTGTTA CACCCTGTGA GCCTGCATGG 
     7261 GATGGATGAC CCGGAGAGAG AAGTATTAGA GTGGAGGTTT GACAGCCGCC TAGCATTTCA 
     7321 TCACATGGCC CGAGAGCTGC ATCCGGACTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG 
     7381 AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC 
     7441 TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT 
     7501 CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGCATG TGAGCAAAAG GCCAGCAAAA 
     7561 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     7621 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     7681 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     7741 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     7801 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     7861 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     7921 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     7981 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     8041 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     8101 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     8161 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     8221 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG ATTACGCCCC GCCCTGCCAC 
     8281 TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC ATCACAAACG 
     8341 GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT ATAATATTTG 
     8401 CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA ATCAAAACTG 
     8461 GTGAAACTCA CCCAGGGATT GGCTGAGACG AAAAACATAT TCTCAATAAA CCCTTTAGGG 
     8521 AAATAGGCCA GGTTTTCACC GTAACACGCC ACATCTTGCG AATATATGTG TAGAAACTGC 
     8581 CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG CTCATGGAAA 
     8641 ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA 
     8701 CGGAACTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC 
     8761 TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG 
     8821 TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG 
     8881 GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA TACTCTTCCT TTTTCAATAT 
     8941 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     9001 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGAC
//