LOCUS       RC209688                8613 bp    DNA     circular  SYN 
DEFINITION  TTBK2 (Myc-DDK-tagged)-Human tau tubulin kinase 2 (TTBK2)
ACCESSION   NM_173500
VERSION     NM_173500.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4770..4861 
                     /label="Myc-DDK"  
     misc_feature    4891..5476 
                     /label="PolyA signal"  
     misc_feature    4948..4967 
                     /label="Restriction Enzyme"  
     misc_feature    5752..6424 
                     /label="ColE1"  
     misc_feature    6854..7648 
                     /label="Kan/Neo"  
     misc_feature    7683..7985 
                     /label="SV40 ori"  
     misc_feature    8071..8548 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4752..4758 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4752 
                     /label="TTBK2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTGGGGGA GGAGAGCAGC TGGATATCCT GAGTGTTGGA ATCCTAGTGA 
     1081 AAGAAAGATG GAAAGTGTTG AGAAAGATTG GGGGTGGGGG CTTTGGAGAA ATTTACGATG 
     1141 CCTTGGACAT GCTCACCAGG GAAAATGTTG CACTGAAGGT GGAATCAGCT CAACAACCAA 
     1201 AACAAGTTCT GAAAATGGAA GTTGCTGTTT TGAAAAAGCT GCAAGGGAAA GACCATGTTT 
     1261 GTAGATTTAT TGGCTGTGGG AGGAATGATC GATTCAACTA TGTGGTCATG CAGTTGCAGG 
     1321 GTCGGAATCT GGCAGATCTT CGCCGTAGCC AGTCCCGAGG CACATTCACC ATTAGTACCA 
     1381 CTCTCCGGCT GGGTAGACAG ATTTTGGAGT CTATTGAAAG CATTCATTCT GTGGGATTCT 
     1441 TGCATCGAGA CATCAAACCG TCGAACTTCG CTATGGGTCG CTTTCCTAGT ACATGTAGGA 
     1501 AATGTTACAT GCTTGATTTT GGCTTGGCTC GACAATTTAC CAATTCCTGT GGTGACGTCA 
     1561 GACCACCTCG AGCTGTGGCA GGTTTTCGAG GGACAGTTCG TTATGCATCA ATCAACGCAC 
     1621 ATCGGAACAG GGAAATGGGA AGACATGATG ACCTTTGGTC CTTATTCTAC ATGTTGGTGG 
     1681 AGTTTGTGGT TGGTCAGCTG CCCTGGAGAA AAATAAAGGA CAAGGAGCAA GTAGGCTCTA 
     1741 TTAAGGAGAG ATATGACCAC AGGCTCATGT TGAAACATCT CCCTCCAGAA TTCAGCATCT 
     1801 TTCTAGACCA TATCTCTTCT TTGGATTATT TTACAAAACC AGACTACCAG CTTCTTACAT 
     1861 CCGTGTTTGA CAATAGCATC AAGACTTTTG GAGTAATTGA GAGTGACCCT TTTGACTGGG 
     1921 AGAAGACTGG AAATGATGGC TCCCTAACAA CCACCACTAC TTCTACCACC CCTCAGTTGC 
     1981 ACACTCGCTT GACCCCTGCT GCAATTGGAA TTGCCAATGC TACTCCCATC CCTGGAGACT 
     2041 TGCTTCGAGA AAATACAGAT GAGGTATTTC CAGATGAACA GCTTAGCGAT GGAGAAAATG 
     2101 GCATCCCTGT TGGTGTGTCA CCAGATAAAT TGCCTGGATC TCTGGGACAC CCCCGTCCCC 
     2161 AGGAGAAGGA TGTTTGGGAA GAGATGGATG CCAACAAAAA CAAGATAAAG CTTGGAATTT 
     2221 GTAAGGCTGC TACTGAAGAG GAGAACAGCC ATGGCCAGGC AAATGGTCTT CTCAATGCTC 
     2281 CAAGCCTTGG GTCACCAATT CGTGTCCGCT CAGAGATTAC TCAGCCAGAC AGAGATATTC 
     2341 CACTGGTGCG AAAGTTACGT TCCATTCACA GCTTTGAGCT GGAAAAACGT CTGACCCTGG 
     2401 AGCCAAAGCC AGACACTGAC AAGTTCCTTG AGACCTGCCT GGAGAAAATG CAGAAAGATA 
     2461 CCAGTGCAGG AAAAGAATCT ATTCTCCCTG CTCTGCTGCA TAAGCCTTGC GTTCCTGCTG 
     2521 TGTCCCGTAC TGACCACATC TGGCACTATG ATGAAGAATA TCTTCCAGAT GCCTCCAAGC 
     2581 CTGCTTCTGC CAACACCCCT GAGCAGGCAG ATGGTGGTGG CAGCAATGGA TTTATAGCTG 
     2641 TTAACCTGAG CTCTTGCAAG CAAGAAATTG ATTCCAAAGA ATGGGTGATT GTGGACAAGG 
     2701 AGCAGGACCT TCAGGATTTT AGGACAAATG AGGCTGTAGG ACATAAAACA ACTGGAAGTC 
     2761 CTTCTGATGA GGAGCCTGAA GTACTTCAAG TCCTGGAGGC ATCACCTCAA GATGAAAAGC 
     2821 TCCAGTTAGG TCCTTGGGCA GAAAATGATC ATTTAAAGAA GGAAACCTCA GGTGTGGTCT 
     2881 TAGCACTTTC TGCAGAGGGT CCTCCTACTG CTGCTTCAGA ACAATATACA GATAGGCTGG 
     2941 AACTCCAGCC TGGAGCTGCT AGTCAGTTTA TTGCAGCGAC GCCCACAAGT CTAATGGAGG 
     3001 CGCAGGCAGA AGGACCCCTT ACAGCGATTA CAATTCCTAG ACCTTCTGTG GCATCTACAC 
     3061 AGTCAACTTC AGGAAGCTTT CACTGTGGTC AGCAGCCAGA GAAGAAAGAT CTTCAGCCCA 
     3121 TGGAGCCCAC TGTGGAACTT TACTCTCCAA GGGAAAACTT CTCTGGCTTG GTTGTGACAG 
     3181 AGGGTGAACC TCCTAGTGGA GGAAGCAGAA CAGATTTGGG GCTTCAGATA GATCACATTG 
     3241 GTCATGACAT GTTACCCAAC ATTAGAGAAA GTAACAAATC TCAAGACCTG GGACCAAAAG 
     3301 AACTTCCTGA TCATAATAGA CTGGTTGTGA GAGAATTTGA AAATCTCCCT GGGGAAACTG 
     3361 AAGAGAAAAG CATCCTTTTA GAGTCAGATA ATGAAGATGA GAAGTTAAGT AGAGGGCAGC 
     3421 ATTGTATTGA GATCTCCTCT CTCCCAGGAG ATTTGGTAAT TGTGGAAAAG GATCACTCAG 
     3481 CTACTACTGA ACCTCTTGAT GTGACAAAAA CACAGACTTT TAGTGTGGTG CCAAATCAAG 
     3541 ACAAAAATAA TGAGATAATG AAGCTTCTGA CAGTTGGAAC TTCAGAAATT TCTTCCAGAG 
     3601 ACATTGACCC ACATGTTGAA GGTCAGATAG GCCAAGTGGC AGAAATGCAA AAAAATAAGA 
     3661 TATCTAAGGA TGATGACATC ATGAGTGAAG ACTTGCCAGG TCATCAAGGA GACCTCTCTA 
     3721 CTTTTTTGCA CCAAGAGGGC AAGAGAGAGA AAATCACCCC TAGAAATGGA GAACTATTTC 
     3781 ATTGTGTTTC AGAGAATGAA CATGGTGCCC CAACCCGGAA GGATATGGTT AGGTCATCCT 
     3841 TTGTAACTAG ACACAGCCGA ATCCCTGTTT TAGCACAAGA GATAGACTCA ACTTTGGAAT 
     3901 CATCCTCTCC AGTTTCTGCA AAAGAAAAGC TCCTCCAAAA GAAAGCCTAT CAGCCAGACC 
     3961 TAGTCAAGCT TCTGGTGGAA AAAAGACAAT TCAAGTCCTT CCTTGGCGAC CTCTCAAGTG 
     4021 CCTCTGATAA ATTGCTAGAG GAGAAACTAG CTACTGTTCC TGCTCCCTTT TGTGAGGAGG 
     4081 AAGTGCTCAC TCCCTTTTCA AGACTGACAG TAGATTCTCA CCTGAGTAGG TCAGCTGAAG 
     4141 ATAGCTTTCT GTCACCCATC ATCTCCCAGT CTAGAAAGAG CAAAATTCCA AGGCCAGTTT 
     4201 CATGGGTCAA CACAGATCAG GTCAATAGCT CAACTTCGTC TCAGTTCTTT CCTCGGCCAC 
     4261 CACCAGGAAA GCCACCCACG AGGCCTGGAG TAGAAGCCAG GCTACGCAGA TATAAAGTCC 
     4321 TAGGGAGTAG TAACTCCGAC TCAGACCTTT TCTCCCGCCT GGCCCAAATT CTTCAAAATG 
     4381 GATCTCAGAA ACCCCGGAGC ACTACTCAGT GCAAGAGTCC AGGATCTCCT CACAATCCAA 
     4441 AAACACCACC CAAGAGTCCA GTTGTCCCTC GCAGGAGTCC CAGTGCCTCT CCTCGAAGCT 
     4501 CATCCTTGCC TCGCACGTCT AGTTCCTCAC CATCTAGGGC TGGACGGCCC CACCATGACC 
     4561 AGAGGAGTTC GTCCCCACAT CTGGGGAGAA GCAAGTCACC TCCCAGCCAC TCAGGATCTT 
     4621 CCTCCTCCAG GAGGTCCTGC CAACAGGAGC ATTGCAAACC CAGCAAGAAT GGCCTGAAAG 
     4681 GATCCGGCAG CCTCCACCAC CACTCAGCCA GCACTAAAAC CCCCCAAGGG AAGAGTAAGC 
     4741 CAGCCAGTAA ACTCAGCAGA ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4801 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4861 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4921 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4981 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5041 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5101 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5161 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5221 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5281 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5341 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5401 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5461 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5521 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5581 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5641 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5701 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5761 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5821 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5881 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5941 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6001 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6061 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6121 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6181 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6241 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6301 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6361 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6421 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6481 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6541 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6601 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6661 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6721 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6781 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6841 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6901 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6961 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7021 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7081 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7141 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7201 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7261 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7321 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7381 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7441 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7501 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7561 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7621 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7681 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7741 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7801 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7861 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7921 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7981 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8041 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8101 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8161 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8221 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8281 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8341 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8401 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8461 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8521 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8581 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//