LOCUS       RC209649                8526 bp    DNA     circular  SYN 
DEFINITION  HDAC6 (Myc-DDK-tagged)-Human histone deacetylase 6 (HDAC6)
ACCESSION   NM_006044
VERSION     NM_006044.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4683..4774 
                     /label="Myc-DDK"  
     misc_feature    4804..5389 
                     /label="PolyA signal"  
     misc_feature    4861..4880 
                     /label="Restriction Enzyme"  
     misc_feature    5665..6337 
                     /label="ColE1"  
     misc_feature    6767..7561 
                     /label="Kan/Neo"  
     misc_feature    7596..7898 
                     /label="SV40 ori"  
     misc_feature    7984..8461 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4665..4671 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4665 
                     /label="HDAC6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCTCAACC GGCCAGGATT CCACCACAAC CAGGCAGCGA AGAAGTAGGC 
     1081 AGAACCCCCA GTCGCCCCCT CAGGACTCCA GTGTCACTTC GAAGCGAAAT ATTAAAAAGG 
     1141 GAGCCGTTCC CCGCTCTATC CCCAATCTAG CGGAGGTAAA GAAGAAAGGC AAAATGAAGA 
     1201 AGCTCGGCCA AGCAATGGAA GAAGACCTAA TCGTGGGACT GCAAGGGATG GATCTGAACC 
     1261 TTGAGGCTGA AGCACTGGCT GGCACTGGCT TGGTGTTGGA TGAGCAGTTA AATGAATTCC 
     1321 ATTGCCTCTG GGATGACAGC TTCCCGGAAG GCCCTGAGCG GCTCCATGCC ATCAAGGAGC 
     1381 AACTGATCCA GGAGGGCCTC CTAGATCGCT GCGTGTCCTT TCAGGCCCGG TTTGCTGAAA 
     1441 AGGAAGAGCT GATGTTGGTT CACAGCCTAG AATATATTGA TCTGATGGAA ACAACCCAGT 
     1501 ACATGAATGA GGGAGAACTC CGTGTCCTAG CAGACACCTA CGACTCAGTT TATCTGCATC 
     1561 CGAACTCATA CTCCTGTGCC TGCCTGGCCT CAGGCTCTGT CCTCAGGCTG GTGGATGCGG 
     1621 TCCTGGGGGC TGAGATCCGG AATGGCATGG CCATCATTAG GCCTCCTGGA CATCACGCCC 
     1681 AGCACAGTCT TATGGATGGC TATTGCATGT TCAACCACGT GGCTGTGGCA GCCCGCTATG 
     1741 CTCAACAGAA ACACCGCATC CGGAGGGTCC TTATCGTAGA TTGGGATGTG CACCACGGTC 
     1801 AAGGAACACA GTTCACCTTC GACCAGGACC CCAGTGTCCT CTATTTCTCC ATCCACCGCT 
     1861 ACGAGCAGGG TAGGTTCTGG CCCCACCTGA AGGCCTCTAA CTGGTCCACC ACAGGTTTCG 
     1921 GCCAAGGCCA AGGATATACC ATCAATGTGC CTTGGAACCA GGTGGGGATG CGGGATGCTG 
     1981 ACTACATTGC TGCTTTCCTG CACGTCCTGC TGCCAGTCGC CCTCGAGTTC CAGCCTCAGC 
     2041 TGGTCCTGGT GGCTGCTGGA TTTGATGCCC TGCAAGGGGA CCCCAAGGGT GAGATGGCCG 
     2101 CCACTCCGGC AGGGTTCGCC CAGCTAACCC ACCTGCTCAT GGGTCTGGCA GGAGGCAAGC 
     2161 TGATCCTGTC TCTGGAGGGT GGCTACAACC TCCGCGCCCT GGCTGAAGGC GTCAGTGCTT 
     2221 CGCTCCACAC CCTTCTGGGA GACCCTTGCC CCATGCTGGA GTCACCTGGT GCCCCCTGCC 
     2281 GGAGTGCCCA GGCTTCAGTT TCCTGTGCTC TGGAAGCCCT TGAGCCCTTC TGGGAGGTTC 
     2341 TTGTGAGATC AACTGAGACC GTGGAGAGGG ACAACATGGA GGAGGACAAT GTAGAGGAGA 
     2401 GCGAGGAGGA AGGACCCTGG GAGCCCCCTG TGCTCCCAAT CCTGACATGG CCAGTGCTAC 
     2461 AGTCTCGCAC AGGGCTGGTC TATGACCAAA ATATGATGAA TCACTGCAAC TTGTGGGACA 
     2521 GCCACCACCC TGAGGTACCC CAGCGCATCT TGCGGATCAT GTGCCGTCTG GAGGAGCTGG 
     2581 GCCTTGCCGG GCGCTGCCTC ACCCTGACAC CGCGCCCTGC CACAGAGGCT GAGCTGCTCA 
     2641 CCTGTCACAG TGCTGAGTAC GTGGGTCATC TCCGGGCCAC AGAGAAAATG AAAACCCGGG 
     2701 AGCTGCACCG TGAGAGTTCC AACTTTGACT CCATCTATAT CTGCCCCAGT ACCTTCGCCT 
     2761 GTGCACAGCT TGCCACTGGC GCTGCCTGCC GCCTGGTGGA GGCTGTGCTC TCAGGAGAGG 
     2821 TTCTGAATGG TGCTGCTGTG GTGCGTCCCC CAGGACACCA CGCAGAGCAG GATGCAGCTT 
     2881 GCGGTTTTTG CTTTTTCAAC TCTGTGGCTG TGGCTGCTCG CCATGCCCAG ACTATCAGTG 
     2941 GGCATGCCCT ACGGATCCTG ATTGTGGATT GGGATGTCCA CCACGGTAAT GGAACTCAGC 
     3001 ACATGTTTGA GGATGACCCC AGTGTGCTAT ATGTGTCCCT GCACCGCTAT GATCATGGCA 
     3061 CCTTCTTCCC CATGGGGGAT GAGGGTGCCA GCAGCCAGAT CGGCCGGGCT GCGGGCACAG 
     3121 GCTTCACCGT CAACGTGGCA TGGAACGGGC CCCGCATGGG TGATGCTGAC TACCTAGCTG 
     3181 CCTGGCATCG CCTGGTGCTT CCCATTGCCT ACGAGTTTAA CCCAGAACTG GTGCTGGTCT 
     3241 CAGCTGGCTT TGATGCTGCA CGGGGGGATC CGCTGGGGGG CTGCCAGGTG TCACCTGAGG 
     3301 GTTATGCCCA CCTCACCCAC CTGCTGATGG GCCTTGCCAG TGGCCGCATT ATCCTTATCC 
     3361 TAGAGGGTGG CTATAACCTG ACATCCATCT CAGAGTCCAT GGCTGCCTGC ACTCGCTCCC 
     3421 TCCTTGGAGA CCCACCACCC CTGCTGACCC TGCCACGGCC CCCACTATCA GGGGCCCTGG 
     3481 CCTCAATCAC TGAGACCATC CAAGTCCATC GCAGATACTG GCGCAGCTTA CGGGTCATGA 
     3541 AGGTAGAAGA CAGAGAAGGA CCCTCCAGTT CTAAGTTGGT CACCAAGAAG GCACCCCAAC 
     3601 CAGCCAAACC TAGGTTAGCT GAGCGGATGA CCACACGAGA AAAGAAGGTT CTGGAAGCAG 
     3661 GCATGGGGAA AGTCACCTCG GCATCATTTG GGGAAGAGTC CACTCCAGGC CAGACTAACT 
     3721 CAGAGACAGC TGTGGTGGCC CTCACTCAGG ACCAGCCCTC AGAGGCAGCC ACAGGGGGAG 
     3781 CCACTCTGGC CCAGACCATT TCTGAGGCAG CCATTGGGGG AGCCATGCTG GGCCAGACCA 
     3841 CCTCAGAGGA GGCTGTCGGG GGAGCCACTC CGGACCAGAC CACCTCAGAG GAGACTGTGG 
     3901 GAGGAGCCAT TCTGGACCAG ACCACCTCAG AGGATGCTGT TGGGGGAGCC ACGCTGGGCC 
     3961 AGACTACCTC AGAGGAGGCT GTAGGAGGAG CTACACTGGC CCAGACCACC TCGGAGGCAG 
     4021 CCATGGAGGG AGCCACACTG GACCAGACTA CGTCAGAGGA GGCTCCAGGG GGCACCGAGC 
     4081 TGATCCAAAC TCCTCTAGCC TCGAGCACAG ACCACCAGAC CCCCCCAACC TCACCTGTGC 
     4141 AGGGAACTAC ACCCCAGATA TCTCCCAGTA CACTGATTGG GAGTCTCAGG ACCTTGGAGC 
     4201 TAGGCAGCGA ATCTCAGGGG GCCTCAGAAT CTCAGGCCCC AGGAGAGGAG AACCTACTAG 
     4261 GAGAGGCAGC TGGAGGTCAG GACATGGCTG ATTCGATGCT GATGCAGGGA TCTAGGGGCC 
     4321 TCACTGATCA GGCCATATTT TATGCTGTGA CACCACTGCC CTGGTGTCCC CATTTGGTGG 
     4381 CAGTATGCCC CATACCTGCA GCAGGCCTAG ACGTGACCCA ACCTTGTGGG GACTGTGGAA 
     4441 CAATCCAAGA GAATTGGGTG TGTCTCTCTT GCTATCAGGT CTACTGTGGT CGTTACATCA 
     4501 ATGGCCACAT GCTCCAACAC CATGGAAATT CTGGACACCC GCTGGTCCTC AGCTACATCG 
     4561 ACCTGTCAGC CTGGTGTTAC TACTGTCAGG CCTATGTCCA CCACCAGGCT CTCCTAGATG 
     4621 TGAAGGACAT CGCCCACCAG AACAAGTTTG GGGAGGATAT GCCCCACCCA CACACGCGTA 
     4681 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4741 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4801 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4861 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4921 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4981 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5041 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5101 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5161 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5221 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5281 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5341 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5401 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5461 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5521 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5581 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5641 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5701 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5761 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5821 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5881 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5941 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6001 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6061 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6121 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6181 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6241 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6301 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6361 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6421 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6481 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6541 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6601 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6661 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6721 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6781 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6841 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6901 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6961 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7021 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7081 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7141 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7201 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7261 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7321 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7381 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7441 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7501 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7561 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7621 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7681 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7741 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7801 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7861 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7921 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7981 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8041 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8101 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8161 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8221 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8281 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8341 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8401 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8461 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8521 AATTTT
//