LOCUS       RC209621                8889 bp    DNA     circular  SYN 
DEFINITION  IGSF1 (Myc-DDK-tagged)-Human immunoglobulin superfamily, member 1 (IGSF1), transcript variant 1
ACCESSION   NM_001555
VERSION     NM_001555.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5046..5137 
                     /label="Myc-DDK"  
     misc_feature    5167..5752 
                     /label="PolyA signal"  
     misc_feature    5224..5243 
                     /label="Restriction Enzyme"  
     misc_feature    6028..6700 
                     /label="ColE1"  
     misc_feature    7130..7924 
                     /label="Kan/Neo"  
     misc_feature    7959..8261 
                     /label="SV40 ori"  
     misc_feature    8347..8824 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5028..5034 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5028 
                     /label="IGSF1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCCTGGAC AGACCAGGGG AGGGGGCCAC CATGCTGAAG ACATTCACTG 
     1081 TTTTGCTCTT TTGCATTCGG ATGAGTCTGG GTATGACATC GATAGTGATG GACCCTCAAC 
     1141 CGGAGTTGTG GATAGAGTCC AACTACCCCC AGGCCCCTTG GGAGAACATC ACGCTTTGGT 
     1201 GCCGAAGCCC CTCTCGGATA TCAAGCAAGT TCCTGCTGCT GAAGGATAAG ACACAGATGA 
     1261 CCTGGATCCG CCCTTCCCAC AAGACCTTCC AAGTTTCATT CCTTATAGGT GCCCTTACTG 
     1321 AGTCCAATGC AGGTCTTTAC CGGTGCTGCT ACTGGAAGGA GACAGGCTGG TCAAAGCCCA 
     1381 GTAAAGTTCT AGAGTTGGAG GCACCAGGCC AACTGCCCAA GCCCATCTTC TGGATTCAGG 
     1441 CTGAGACCCC CGCTCTTCCT GGGTGTAATG TTAACATCCT CTGCCATGGC TGGCTGCAGG 
     1501 ATTTGGTATT CATGCTGTTT AAAGAGGGAT ATGCAGAGCC TGTGGATTAC CAAGTCCCAA 
     1561 CTGGGACAAT GGCCATATTC TCCATTGACA ACCTGACACC TGAGGATGAA GGGGTTTACA 
     1621 TCTGCCGCAC TCATATCCAG ATGCTCCCCA CCCTGTGGTC AGAGCCCAGC AACCCCCTGA 
     1681 AGCTGGTTGT AGCAGGACTC TACCCCAAAC CAACTTTGAC AGCCCATCCT GGGCCCATCA 
     1741 TGGCACCTGG AGAAAGCCTG AATCTCAGGT GCCAAGGGCC AATCTATGGA ATGACCTTTG 
     1801 CTCTAATGAG GGTTGAAGAC TTGGAGAAGT CCTTTTACCA CAAGAAGACA ATAAAAAATG 
     1861 AGGCAAATTT CTTCTTCCAG TCTTTGAAGA TCCAAGATAC TGGACATTAC CTCTGTTTTT 
     1921 ACTATGACGC ATCATATAGA GGTTCACTCC TTAGTGATGT CCTGAAAATC TGGGTAACTG 
     1981 ACACTTTCCC CAAGACCTGG CTACTTGCTC GGCCCAGTGC TGTGGTCCAA ATGGGTCAGA 
     2041 ATGTGAGCCT ACGGTGTCGA GGACCAGTGG ATGGAGTGGG TCTTGCACTC TATAAGAAAG 
     2101 GAGAAGACAA ACCACTTCAA TTTTTGGATG CCACCAGCAT CGATGACAAC ACATCATTCT 
     2161 TCCTCAACAA TGTAACCTAC AGTGATACTG GCATCTATAG CTGCCACTAT CTTCTCACCT 
     2221 GGAAGACCTC CATTAGGATG CCATCACACA ACACTGTGGA GCTTATGGTT GTAGATAAGC 
     2281 CCCCCAAACC CTCCCTGTCA GCTTGGCCAA GCACTGTGTT CAAGCTAGGA AAGGCCATCA 
     2341 CCCTTCAGTG CCGAGTATCT CATCCAGTAC TGGAATTTTC TCTGGAATGG GAAGAAAGAG 
     2401 AAACATTCCA AAAATTCTCA GTAAACGGAG ACTTCATCAT CAGTAATGTT GACGGGAAAG 
     2461 GCACAGGGAC CTACAGTTGC AGCTATCGCG TAGAGACACA TCCTAACATC TGGTCACATC 
     2521 GCAGTGAGCC CCTGAAGCTG ATGGGGCCAG CAGGCTATCT CACCTGGAAT TACGTTCTGA 
     2581 ATGAAGCTAT CAGGTTGTCT CTAATCATGC AGCTTGTTGC CTTGCTGTTG GTAGTGCTGT 
     2641 GGATAAGGTG GAAGTGTCGG AGACTCAGAA TCAGAGAAGC CTGGTTGCTG GGAACAGCTC 
     2701 AAGGGGTCAC CATGCTCTTC ATAGTCACGG CCCTTCTCTG CTGTGGACTG TGCAATGGGG 
     2761 TATTGATAGA AGAGACTGAA ATAGTCATGC CAACCCCTAA GCCTGAGCTG TGGGCAGAGA 
     2821 CCAACTTTCC TCTGGCCCCG TGGAAGAACT TAACCCTCTG GTGCAGAAGC CCTTCTGGCT 
     2881 CAACTAAGGA GTTTGTGTTG CTGAAGGATG GGACCGGGTG GATCGCCACT CGCCCGGCCT 
     2941 CAGAGCAGGT CCGGGCTGCC TTCCCCCTTG GCGCCCTGAC CCAGAGCCAC ACCGGGAGCT 
     3001 ACCACTGCCA TTCATGGGAG GAGATGGCTG TATCGGAGCC CAGTGAGGCA CTTGAGCTGG 
     3061 TGGGGACAGA CATCCTCCCC AAACCTGTCA TTTCTGCTTC CCCCACAATC CGGGGCCAGG 
     3121 AACTACAACT CCGGTGCAAA GGATGGCTGG CAGGCATGGG GTTTGCTCTG TATAAGGAGG 
     3181 GAGAGCAAGA ACCTGTCCAG CAACTTGGTG CTGTTGGAAG AGAAGCCTTC TTTACAATCC 
     3241 AGAGAATGGA GGATAAAGAC GAAGGCAATT ACAGCTGCCG CACTCACACT GAAAAACGCC 
     3301 CCTTCAAGTG GTCTGAGCCC AGTGAGCCGC TGGAGCTTGT CATAAAAGAA ATGTACCCTA 
     3361 AGCCCTTCTT CAAGACATGG GCCAGCCCTG TGGTCACCCC TGGTGCCCGA GTGACTTTCA 
     3421 ATTGCTCCAC CCCCCACCAG CATATGAGCT TTATTCTTTA CAAAGATGGA AGTGAAATAG 
     3481 CATCCAGTGA CAGGTCCTGG GCAAGTCCGG GGGCCAGTGC AGCTCACTTT CTAATCATTT 
     3541 CGGTGGGCAT TGGTGATGGA GGGAATTACA GCTGCCGATA TTACGACTTT TCTATCTGGT 
     3601 CTGAGCCCAG CGACCCTGTG GAGCTCGTGG TGACAGAATT CTACCCCAAA CCCACTCTCC 
     3661 TGGCACAGCC AGGTCCTGTG GTGTTTCCTG GGAAGAGTGT GATCCTGCGC TGCCAAGGGA 
     3721 CTTTCCAGGG CATGAGGTTC GCCCTCTTGC AGGAGGGAGC CCATGTTCCC TTACAGTTTC 
     3781 GGAGTGTCTC AGGGAACTCA GCTGACTTCC TTCTCCACAC TGTTGGAGCA GAGGACTCTG 
     3841 GGAACTATAG CTGTATCTAC TATGAGACAA CCATGTCAAA CAGGGGGTCA TATCTCAGTA 
     3901 TGCCCCTTAT GATCTGGGTG ACTGACACAT TCCCTAAGCC ATGGTTGTTT GCTGAGCCCA 
     3961 GTTCTGTGGT TCCCATGGGG CAGAATGTTA CTCTCTGGTG CCGAGGGCCG GTCCATGGAG 
     4021 TAGGATACAT TCTGCACAAA GAAGGAGAAG CCACTTCAAT GCAGCTCTGG GGATCCACCA 
     4081 GTAATGACGG GGCATTCCCC ATCACCAATA TATCTGGTAC TAGCATGGGG CGTTACAGCT 
     4141 GCTGCTACCA CCCTGACTGG ACCAGTTCTA TCAAGATACA ACCTAGCAAC ACCCTGGAAC 
     4201 TCCTAGTCAC AGGCTTACTC CCCAAACCCA GCCTATTAGC CCAGCCTGGT CCCATGGTGG 
     4261 CCCCTGGCGA AAATATGACT CTTCAGTGTC AAGGGGAACT GCCAGACTCA ACATTTGTCC 
     4321 TGTTGAAGGA GGGGGCTCAG GAGCCTTTAG AGCAACAGAG GCCAAGTGGG TACAGGGCTG 
     4381 ACTTCTGGAT GCCAGCAGTG AGAGGTGAAG ACTCTGGGAT CTATAGCTGT GTTTATTATT 
     4441 TGGACTCTAC TCCCTTTGCA GCTTCAAATC ACAGTGACTC CCTGGAGATC TGGGTGACTG 
     4501 ATAAGCCCCC TAAACCCTCT CTGTCAGCCT GGCCCAGCAC CATGTTCAAG TTAGGGAAGG 
     4561 ACATCACCCT TCAGTGCCGA GGACCCCTGC CAGGTGTTGA ATTTGTCCTA GAACATGATG 
     4621 GAGAAGAAGC ACCTCAGCAG TTTTCAGAGG ATGGAGACTT TGTCATCAAC AACGTAGAAG 
     4681 GAAAAGGCAT TGGAAACTAC AGCTGCAGCT ACCGCCTCCA GGCCTACCCT GATATCTGGT 
     4741 CAGAGCCTAG TGATCCCCTG GAGCTGGTGG GGGCAGCAGG GCCTGTTGCT CAGGAGTGCA 
     4801 CTGTAGGGAA CATTGTCCGA AGTAGCCTAA TCGTGGTGGT TGTTGTAGCC TTGGGGGTAG 
     4861 TGCTAGCCAT AGAGTGGAAG AAGTGGCCTC GACTGCGAAC CAGAGGCTCA GAGACAGACG 
     4921 GAAGAGACCA GACCATTGCC CTTGAAGAGT GTAACCAAGA AGGAGAACCA GGCACCCCTG 
     4981 CCAATTCTCC TTCATCAACC TCTCAGAGAA TCTCTGTGGA ACTGCCCGTT CCAATAACGC 
     5041 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     5101 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5161 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5221 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5281 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5341 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5401 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5461 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5521 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5581 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5641 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5701 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5761 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5821 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5881 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5941 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     6001 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6061 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6121 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6181 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6241 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6301 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6361 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6421 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6481 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6541 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6601 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6661 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6721 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6781 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6841 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6901 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6961 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     7021 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     7081 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7141 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7201 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7261 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7321 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7381 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7441 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7501 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7561 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7621 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7681 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7741 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7801 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7861 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7921 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7981 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     8041 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     8101 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8161 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8221 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8281 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8341 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8401 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8461 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8521 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8581 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8641 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8701 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8761 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8821 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8881 GCGAATTTT
//