LOCUS       RC209595                8298 bp    DNA     circular  SYN 
DEFINITION  HMHA1 (Myc-DDK-tagged)-Human histocompatibility (minor) HA-1 (HMHA1)
ACCESSION   NM_012292
VERSION     NM_012292.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4453..4544 
                     /label="Myc-DDK"  
     misc_feature    4574..5159 
                     /label="PolyA signal"  
     misc_feature    4631..4650 
                     /label="Restriction Enzyme"  
     misc_feature    5435..6107 
                     /label="ColE1"  
     misc_feature    6537..7331 
                     /label="Kan/Neo"  
     misc_feature    7366..7668 
                     /label="SV40 ori"  
     misc_feature    7754..8231 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4428..4435 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4428 
                     /label="ARHGAP45"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTCTCCAGG AAGAAACGAG AGCTCATGAA AACCCCTTCC ATCTCGAAAA 
     1081 AGAACCGCGC GGGAAGCCCC AGCCCGCAGC CCTCGGGGGA GCTGCCCAGG AAGGATGGGG 
     1141 CTGACGCGGT GTTCCCCGGA CCAAGCCTGG AGCCGCCCGC TGGGTCCTCC GGCGTCAAGG 
     1201 CCACAGGGAC CCTCAAGCGG CCCACCAGCC TGAGCCGCCA CGCCAGCGCG GCTGGCTTCC 
     1261 CCCTGTCGGG TGCTGCCTCC TGGACACTGG GCCGGAGCCA CCGGAGCCCA CTGACAGCCG 
     1321 CCAGCCCGGG CGAGCTGCCC ACCGAGGGTG CCGGCCCGGA CGTCGTCGAG GACATCTCCC 
     1381 ATCTGCTGGC GGACGTGGCC CGCTTCGCTG AGGGCCTTGA GAAACTTAAG GAGTGTGTGC 
     1441 TGCATGACGA CCTCCTTGAG GCCCGCCGCC CGCGGGCCCA CGAGTGCCTG GGTGAGGCTC 
     1501 TGCGTGTCAT GCATCAGATC ATCTCCAAGT ACCCGCTGCT GAACACCGTG GAGACGCTCA 
     1561 CCGCAGCCGG CACCCTCATT GCCAAGGTCA AAGCCTTCCA TTATGAGAGC AACAATGATC 
     1621 TGGAGAAACA GGAGTTCGAG AAGGCCCTGG AGACGATTGC TGTGGCCTTC AGTAGCACAG 
     1681 TGTCCGAGTT CCTCATGGGT GAAGTGGACA GCAGCACCCT CCTAGCAGTG CCTCCTGGGG 
     1741 ACTCGAGCCA GTCCATGGAA AGCCTGTATG GACCGGGCAG TGAGGGCACG CCTCCCAGCC 
     1801 TGGATGACTG TGACGCCGGC TGCCTGCCCG CCGAGGAGGT GGACGTGCTG CTACAGCGCT 
     1861 GTGAGGGGGG CGTGGATGCC GCACTGCTGT ATGCCAAGAA CATGGCCAAG TACATGAAGG 
     1921 ACCTCATCAG CTACCTGGAG AAGCGGACGA CGCTGGAGAT GGAGTTTGCC AAGGGCCTGC 
     1981 AGAAGATCGC TCACAACTGC AGACAGAGCG TCATGCAGGA GCCCCACATG CCGCTCCTGT 
     2041 CCATCTACTC GCTGGCCCTG GAGCAGGACC TGGAGTTCGG CCACAGCATG GTGCAGGCGG 
     2101 TGGGCACCTT GCAGACCCAG ACCTTCATGC AGCCCCTGAC CCTGCGGCGG CTTGAACACG 
     2161 AGAAGCGCAG GAAGGAGATC AAGGAGGCCT GGCACCGTGC CCAGAGGAAG CTGCAAGAGG 
     2221 CGGAGTCCAA CCTGCGCAAG GCCAAGCAGG GTTACGTGCA GCGCTGCGAG GACCACGACA 
     2281 AGGCTCGCTT CCTCGTGGCC AAGGCGGAGG AGGAGCAGGC TGGCAGCGCG CCGGGAGCAG 
     2341 GCGGCACGGC CACCAAGACC CTGGACAAGC GGCGGCGGCT GGAGGAGGAG GCCAAGAACA 
     2401 AGGCGGAGGA AGCTATGGCC ACCTACCGCA CCTGCGTGGC CGACGCGAAG ACGCAGAAGC 
     2461 AGGAGCTGGA GGATACCAAG GTGACGGCGC TGCGGCAGAT CCAGGAGGTC ATCCGGCAGA 
     2521 GCGACCAAAC CATCAAGTCG GCCACGATCT CCTACTACCA GATGATGCAT ATGCAGACGG 
     2581 CGCCGCTGCC CGTGCACTTC CAGATGCTGT GTGAGAGCAG CAAGCTGTAT GACCCAGGCC 
     2641 AGCAGTACGC CTCCCACGTG CGCCAGCTGC AGCGGGACCA GGAGCCCGAT GTGCACTACG 
     2701 ACTTTGAGCC CCACGTCTCC GCCAACGCCT GGTCCCCCGT CATGCGTGCC CGGAAGAGCA 
     2761 GCTTCAACGT GAGTGATGTG GCGCGGCCCG AGGCTGCCGG GAGCCCCCCA GAAGAAGGCG 
     2821 GGTGCACTGA GGGCACACCT GCCAAGGACC ACAGGGCCGG GCGAGGACAC CAGGTTCACA 
     2881 AGTCATGGCC GCTCTCGATC TCAGACTCGG ACAGTGGGCT GGACCCCGGC CCTGGCGCAG 
     2941 GGGACTTTAA GAAGTTCGAG CGGACGTCAT CCAGTGGTAC CATGTCGTCC ACGGAGGAGC 
     3001 TGGTGGACCC AGACGGTGGA GCCGGGGCTT CAGCCTTTGA GCAGGCTGAC CTCAACGGCA 
     3061 TGACCCCCGA GCTGCCGGTG GCCGTGCCCA GTGGACCGTT CCGCCACGAG GGGCTGTCCA 
     3121 AGGCGGCCCG TACTCACCGG CTCCGGAAGC TCCGCACGCC CGCCAAGTGC CGCGAGTGCA 
     3181 ACAGCTACGT CTACTTCCAG GGTGCTGAGT GTGAAGAGTG CTGCCTGGCC TGCCACAAGA 
     3241 AATGTCTGGA GACGCTGGCC ATACAGTGCG GGCACAAGAA GCTGCAGGGC CGCCTGCAGC 
     3301 TGTTCGGCCA GGACTTCAGC CACGCGGCCC GCAGCGCCCC CGACGGCGTG CCCTTCATCG 
     3361 TCAAGAAGTG CGTCTGCGAG ATCGAGCGGC GGGCGCTGCG CACCAAGGGC ATCTACCGGG 
     3421 TCAATGGGGT AAAGACACGC GTGGAGAAGC TGTGCCAGGC CTTCGAGAAC GGCAAGGAGC 
     3481 TGGTCGAGCT GTCGCAGGCC TCGCCCCACG ACATCAGCAA CGTCCTCAAG CTCTACCTGC 
     3541 GTCAGCTTCC CGAGCCGCTC ATCTCCTTCC GCCTCTACCA CGAGCTCGTA GGGCTGGCCA 
     3601 AGGACAGCCT GAAGGCAGAG GCCGAGGCCA AGGCGGCGTC CCGGGGCCGG CAGGACGGCT 
     3661 CGGAGAGCGA GGCAGTGGCG GTGGCCCTGG CAGGTCGGCT GCGGGAGCTC CTGCGGGACC 
     3721 TGCCGCCTGA GAACCGGGCC TCGCTGCAGT ACCTGCTGCG TCACCTACGC AGGATCGTGG 
     3781 AGGTGGAGCA GGACAACAAG ATGACCCCCG GGAACCTGGG CATCGTGTTC GGGCCCACGC 
     3841 TGCTTCGGCC ACGGCCCACC GAGGCCACCG TGTCCCTCTC CTCCCTGGTG GATTATCCCC 
     3901 ATCAGGCCCG CGTCATCGAG ACTCTCATCG TCCACTACGG CCTGGTCTTC GAGGAGGAGC 
     3961 CGGAGGAGAC CCCCGGGGGC CAGGACGAGT CATCCAACCA GCGAGCTGAG GTAGTCGTCC 
     4021 AGGTGCCGTA CCTGGAGGCG GGCGAGGCGG TGGTCTACCC GCTGCAGGAG GCGGCGGCGG 
     4081 ACGGGTGCAG AGAATCCCGA GTTGTGTCCA ACGATTCGGA CTCGGACCTA GAGGAGGCCT 
     4141 CCGAGCTGCT GTCCTCATCG GAGGCCAGTG CCCTGGGCCA CCTCAGCTTC CTGGAGCAGC 
     4201 AGCAGAGCGA GGCCAGCCTA GAGGTGGCTT CTGGCAGCCA CAGCGGCAGT GAGGAGCAGC 
     4261 TGGAGGCCAC AGCCCGGGAG GACGGGGACG GGGACGAGGA CGGCCCGGCC CAGCAGCTCT 
     4321 CAGGATTCAA CACCAACCAG TCCAACAACG TGCTGCAGGC CCCACTGCCC CCCATGAGGC 
     4381 TCCGTGGCGG GCGGATGACA CTGGGCTCCT GCAGGGAAAG GCAGCCGGAA TTCGTGAGCG 
     4441 GACCGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4501 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4561 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4621 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4681 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4741 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4801 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4861 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4921 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4981 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5041 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5101 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5161 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5221 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5281 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5341 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5401 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5461 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5521 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5581 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5641 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5701 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5761 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5821 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5881 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5941 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6001 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6061 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6121 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6181 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6241 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6301 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6361 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6421 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6481 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6541 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6601 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6661 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6721 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6781 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6841 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6901 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6961 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7021 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7081 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7141 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7201 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7261 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7321 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7381 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7441 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7501 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7561 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7621 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7681 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7741 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7801 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7861 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7921 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7981 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8041 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8101 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8161 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8221 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8281 AAATTTAACG CGAATTTT
//