LOCUS       RC209575L1                8628 bp    DNA     circular  SYN 
DEFINITION  APP (Myc-DDK-tagged)-Human amyloid beta (A4) precursor protein (APP), transcript variant 2
ACCESSION   NM_201413
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    6919..7099 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    7827..8486 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7258..7826 
                     /label="ColE1"  
     misc_feature    5794..5814 
                     /label="LR50 primer"  
     misc_feature    5591..5662 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    5803..6394 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5573..5579 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5573 
                     /label="APP"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GCTGCCCGGT TTGGCACTGC TCCTGCTGGC 
     3361 CGCCTGGACG GCTCGGGCGC TGGAGGTACC CACTGATGGT AATGCTGGCC TGCTGGCTGA 
     3421 ACCCCAGATT GCCATGTTCT GTGGCAGACT GAACATGCAC ATGAATGTCC AGAATGGGAA 
     3481 GTGGGATTCA GATCCATCAG GGACCAAAAC CTGCATTGAT ACCAAGGAAG GCATCCTGCA 
     3541 GTATTGCCAA GAAGTCTACC CTGAACTGCA GATCACCAAT GTGGTAGAAG CCAACCAACC 
     3601 AGTGACCATC CAGAACTGGT GCAAGCGGGG CCGCAAGCAG TGCAAGACCC ATCCCCACTT 
     3661 TGTGATTCCC TACCGCTGCT TAGTTGGTGA GTTTGTAAGT GATGCCCTTC TCGTTCCTGA 
     3721 CAAGTGCAAA TTCTTACACC AGGAGAGGAT GGATGTTTGC GAAACTCATC TTCACTGGCA 
     3781 CACCGTCGCC AAAGAGACAT GCAGTGAGAA GAGTACCAAC TTGCATGACT ACGGCATGTT 
     3841 GCTGCCCTGC GGAATTGACA AGTTCCGAGG GGTAGAGTTT GTGTGTTGCC CACTGGCTGA 
     3901 AGAAAGTGAC AATGTGGATT CTGCTGATGC GGAGGAGGAT GACTCGGATG TCTGGTGGGG 
     3961 CGGAGCAGAC ACAGACTATG CAGATGGGAG TGAAGACAAA GTAGTAGAAG TAGCAGAGGA 
     4021 GGAAGAAGTG GCTGAGGTGG AAGAAGAAGA AGCCGATGAT GACGAGGACG ATGAGGATGG 
     4081 TGATGAGGTA GAGGAAGAGG CTGAGGAACC CTACGAAGAA GCCACAGAGA GAACCACCAG 
     4141 CATTGCCACC ACCACCACCA CCACCACAGA GTCTGTGGAA GAGGTGGTTC GAGAGGTGTG 
     4201 CTCTGAACAA GCCGAGACGG GGCCGTGCCG AGCAATGATC TCCCGCTGGT ACTTTGATGT 
     4261 GACTGAAGGG AAGTGTGCCC CATTCTTTTA CGGCGGATGT GGCGGCAACC GGAACAACTT 
     4321 TGACACAGAA GAGTACTGCA TGGCCGTGTG TGGCAGCGCC ATTCCTACAA CAGCAGCCAG 
     4381 TACCCCTGAT GCCGTTGACA AGTATCTCGA GACACCTGGG GATGAGAATG AACATGCCCA 
     4441 TTTCCAGAAA GCCAAAGAGA GGCTTGAGGC CAAGCACCGA GAGAGAATGT CCCAGGTCAT 
     4501 GAGAGAATGG GAAGAGGCAG AACGTCAAGC AAAGAACTTG CCTAAAGCTG ATAAGAAGGC 
     4561 AGTTATCCAG CATTTCCAGG AGAAAGTGGA ATCTTTGGAA CAGGAAGCAG CCAACGAGAG 
     4621 ACAGCAGCTG GTGGAGACAC ACATGGCCAG AGTGGAAGCC ATGCTCAATG ACCGCCGCCG 
     4681 CCTGGCCCTG GAGAACTACA TCACCGCTCT GCAGGCTGTT CCTCCTCGGC CTCGTCACGT 
     4741 GTTCAATATG CTAAAGAAGT ATGTCCGCGC AGAACAGAAG GACAGACAGC ACACCCTAAA 
     4801 GCATTTCGAG CATGTGCGCA TGGTGGATCC CAAGAAAGCC GCTCAGATCC GGTCCCAGGT 
     4861 TATGACACAC CTCCGTGTGA TTTATGAGCG CATGAATCAG TCTCTCTCCC TGCTCTACAA 
     4921 CGTGCCTGCA GTGGCCGAGG AGATTCAGGA TGAAGTTGAT GAGCTGCTTC AGAAAGAGCA 
     4981 AAACTATTCA GATGACGTCT TGGCCAACAT GATTAGTGAA CCAAGGATCA GTTACGGAAA 
     5041 CGATGCTCTC ATGCCATCTT TGACCGAAAC GAAAACCACC GTGGAGCTCC TTCCCGTGAA 
     5101 TGGAGAGTTC AGCCTGGACG ATCTCCAGCC GTGGCATTCT TTTGGGGCTG ACTCTGTGCC 
     5161 AGCCAACACA GAAAACGAAG TTGAGCCTGT TGATGCCCGC CCTGCTGCCG ACCGAGGACT 
     5221 GACCACTCGA CCAGGTTCTG GGTTGACAAA TATCAAGACG GAGGAGATCT CTGAAGTGAA 
     5281 GATGGATGCA GAATTCCGAC ATGACTCAGG ATATGAAGTT CATCATCAAA AATTGGTGTT 
     5341 CTTTGCAGAA GATGTGGGTT CAAACAAAGG TGCAATCATT GGACTCATGG TGGGCGGTGT 
     5401 TGTCATAGCG ACAGTGATCG TCATCACCTT GGTGATGCTG AAGAAGAAAC AGTACACATC 
     5461 CATTCATCAT GGTGTGGTGG AGGTTGACGC CGCTGTCACC CCAGAGGAGC GCCACCTGTC 
     5521 CAAGATGCAG CAGAACGGCT ACGAAAATCC AACCTACAAG TTCTTTGAGC AGATGCAGAA 
     5581 CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA 
     5641 TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCTGTACAA 
     5701 GTAGGATTCG TCGAGGGACC TAATAACTTC GTATAGCATA CATTATACGA AGTTATACAT 
     5761 GTTTAAGGGT TCCGGTTCCA CTAGGTACAA TTCGATATCA AGCTTATCGA TAATCAACCT 
     5821 CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG 
     5881 CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC 
     5941 ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT 
     6001 GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC 
     6061 ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG 
     6121 GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT 
     6181 GACAATTCCG TGGTGTTGTC GGGGAAATCA TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT 
     6241 GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG 
     6301 GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC 
     6361 CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC ATCGATACCG TCGACCTCGA 
     6421 TCGAGACCTA GAAAAACATG GAGCAATCAC AAGTAGCAAT ACAGCAGCTA CCAATGCTGA 
     6481 TTGTGCCTGG CTAGAAGCAC AAGAGGAGGA GGAGGTGGGT TTTCCAGTCA CACCTCAGGT 
     6541 ACCTTTAAGA CCAATGACTT ACAAGGCAGC TGTAGATCTT AGCCACTTTT TAAAAGAAAA 
     6601 GGGGGGACTG GAAGGGCTAA TTCACTCCCA ACGAAGACAA GATATCCTTG ATCTGTGGAT 
     6661 CTACCACACA CAAGGCTACT TCCCTGATTG GCAGAACTAC ACACCAGGGC CAGGGATCAG 
     6721 ATATCCACTG ACCTTTGGAT GGTGCTACAA GCTAGTACCA GTTGAGCAAG AGAAGGTAGA 
     6781 AGAAGCCAAT GAAGGAGAGA ACACCCGCTT GTTACACCCT GTGAGCCTGC ATGGGATGGA 
     6841 TGACCCGGAG AGAGAAGTAT TAGAGTGGAG GTTTGACAGC CGCCTAGCAT TTCATCACAT 
     6901 GGCCCGAGAG CTGCATCCGG ACTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG 
     6961 GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT 
     7021 GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC 
     7081 CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     7141 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     7201 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     7261 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     7321 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     7381 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     7441 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     7501 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     7561 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     7621 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     7681 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     7741 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     7801 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGATTACG CCCCGCCCTG CCACTCATCG 
     7861 CAGTACTGTT GTAATTCATT AAGCATTCTG CCGACATGGA AGCCATCACA AACGGCATGA 
     7921 TGAACCTGAA TCGCCAGCGG CATCAGCACC TTGTCGCCTT GCGTATAATA TTTGCCCATG 
     7981 GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT TTAAATCAAA ACTGGTGAAA 
     8041 CTCACCCAGG GATTGGCTGA GACGAAAAAC ATATTCTCAA TAAACCCTTT AGGGAAATAG 
     8101 GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA TGTGTAGAAA CTGCCGGAAA 
     8161 TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG TTTGCTCATG GAAAACGGTG 
     8221 TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT CTTTCATTGC CATACGGAAC 
     8281 TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA AGGCCGGATA AAACTTGTGC 
     8341 TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA GCTGAACGGT CTGGTTATAG 
     8401 GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT TACGATGCCA TTGGGATATA 
     8461 TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATACTCT TCCTTTTTCA ATATTATTGA 
     8521 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8581 AAACAAATAG GGGTCCCGCG CACATTTCCC CGAAAAGTGC CACCTGAC
//