LOCUS       RC209563L2                9444 bp    DNA     circular  SYN 
DEFINITION  HSP90B1 (Myc-DDK-tagged)-Human heat shock protein 90kDa beta (Grp94), member 1 (HSP90B1)
ACCESSION   NM_003299
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7735..7915 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8643..9302 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7963..8582 
                     /label="ColE1"  
     misc_feature    6610..6630 
                     /label="LR50"  
     misc_feature    5750..6475 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    6619..7210 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5729..5735 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5729 
                     /label="HSP90B1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAGGGCCCTG TGGGTGCTGG GCCTCTGCTG 
     3361 CGTCCTGCTG ACCTTCGGGT CGGTCAGAGC TGACGATGAA GTTGATGTGG ATGGTACAGT 
     3421 AGAAGAGGAT CTGGGTAAAA GTAGAGAAGG ATCAAGGACG GATGATGAAG TAGTACAGAG 
     3481 AGAGGAAGAA GCTATTCAGT TGGATGGATT AAATGCATCA CAAATAAGAG AACTTAGAGA 
     3541 GAAGTCGGAA AAGTTTGCCT TCCAAGCCGA AGTTAACAGA ATGATGAAAC TTATCATCAA 
     3601 TTCATTGTAT AAAAATAAAG AGATTTTCCT GAGAGAACTG ATTTCAAATG CTTCTGATGC 
     3661 TTTAGATAAG ATAAGGCTAA TATCACTGAC TGATGAAAAT GCTCTTTCTG GAAATGAGGA 
     3721 ACTAACAGTC AAAATTAAGT GTGATAAGGA GAAGAACCTG CTGCATGTCA CAGACACCGG 
     3781 TGTAGGAATG ACCAGAGAAG AGTTGGTTAA AAACCTTGGT ACCATAGCCA AATCTGGGAC 
     3841 AAGCGAGTTT TTAAACAAAA TGACTGAAGC ACAGGAAGAT GGCCAGTCAA CTTCTGAATT 
     3901 GATTGGCCAG TTTGGTGTCG GTTTCTATTC CGCCTTCCTT GTAGCAGATA AGGTTATTGT 
     3961 CACTTCAAAA CACAACAACG ATACCCAGCA CATCTGGGAG TCTGACTCCA ATGAATTTTC 
     4021 TGTAATTGCT GACCCAAGAG GAAACACTCT AGGACGGGGA ACGACAATTA CCCTTGTCTT 
     4081 AAAAGAAGAA GCATCTGATT ACCTTGAATT GGATACAATT AAAAATCTCG TCAAAAAATA 
     4141 TTCACAGTTC ATAAACTTTC CTATTTATGT ATGGAGCAGC AAGACTGAAA CTGTTGAGGA 
     4201 GCCCATGGAG GAAGAAGAAG CAGCCAAAGA AGAGAAAGAA GAATCTGATG ATGAAGCTGC 
     4261 AGTAGAGGAA GAAGAAGAAG AAAAGAAACC AAAGACTAAA AAAGTTGAAA AAACTGTCTG 
     4321 GGACTGGGAA CTTATGAATG ATATCAAACC AATATGGCAG AGACCATCAA AAGAAGTAGA 
     4381 AGAAGATGAA TACAAAGCTT TCTACAAATC ATTTTCAAAG GAAAGTGATG ACCCCATGGC 
     4441 TTATATTCAC TTTACTGCTG AAGGGGAAGT TACCTTCAAA TCAATTTTAT TTGTACCCAC 
     4501 ATCTGCTCCA CGTGGTCTGT TTGACGAATA TGGATCTAAA AAGAGCGATT ACATTAAGCT 
     4561 CTATGTGCGC CGTGTATTCA TCACAGACGA CTTCCATGAT ATGATGCCTA AATACCTCAA 
     4621 TTTTGTCAAG GGTGTGGTGG ACTCAGATGA TCTCCCCTTG AATGTTTCCC GCGAGACTCT 
     4681 TCAGCAACAT AAACTGCTTA AGGTGATTAG GAAGAAGCTT GTTCGTAAAA CGCTGGACAT 
     4741 GATCAAGAAG ATTGCTGATG ATAAATACAA TGATACTTTT TGGAAAGAAT TTGGTACCAA 
     4801 CATCAAGCTT GGTGTGATTG AAGACCACTC GAATCGAACA CGTCTTGCTA AACTTCTTAG 
     4861 GTTCCAGTCT TCTCATCATC CAACTGACAT TACTAGCCTA GACCAGTATG TGGAAAGAAT 
     4921 GAAGGAAAAA CAAGACAAAA TCTACTTCAT GGCTGGGTCC AGCAGAAAAG AGGCTGAATC 
     4981 TTCTCCATTT GTTGAGCGAC TTCTGAAAAA GGGCTATGAA GTTATTTACC TCACAGAACC 
     5041 TGTGGATGAA TACTGTATTC AGGCCCTTCC CGAATTTGAT GGGAAGAGGT TCCAGAATGT 
     5101 TGCCAAGGAA GGAGTGAAGT TCGATGAAAG TGAGAAAACT AAGGAGAGTC GTGAAGCAGT 
     5161 TGAGAAAGAA TTTGAGCCTC TGCTGAATTG GATGAAAGAT AAAGCCCTTA AGGACAAGAT 
     5221 TGAAAAGGCT GTGGTGTCTC AGCGCCTGAC AGAATCTCCG TGTGCTTTGG TGGCCAGCCA 
     5281 GTACGGATGG TCTGGCAACA TGGAGAGAAT CATGAAAGCA CAAGCGTACC AAACGGGCAA 
     5341 GGACATCTCT ACAAATTACT ATGCGAGTCA GAAGAAAACA TTTGAAATTA ATCCCAGACA 
     5401 CCCGCTGATC AGAGACATGC TTCGACGAAT TAAGGAAGAT GAAGATGATA AAACAGTTTT 
     5461 GGATCTTGCT GTGGTTTTGT TTGAAACAGC AACGCTTCGG TCAGGGTATC TTTTACCAGA 
     5521 CACTAAAGCA TATGGAGATA GAATAGAAAG AATGCTTCGC CTCAGTTTGA ACATTGACCC 
     5581 TGATGCAAAG GTGGAAGAAG AGCCTGAAGA AGAACCTGAA GAGACAGCAG AAGACACAAC 
     5641 AGAAGACACA GAGCAAGACG AAGATGAAGA AATGGATGTG GGAACAGATG AAGAAGAAGA 
     5701 AACAGCAAAG GAATCTACAG CTGAAAAAGA TGAATTGACG CGTACGCGGC CGCTCGAGAT 
     5761 GAGCGGGGGC GAGGAGCTGT TCGCCGGCAT CGTGCCCGTG CTGATCGAGC TGGACGGCGA 
     5821 CGTGCACGGC CACAAGTTCA GCGTGCGCGG CGAGGGCGAG GGCGACGCCG ACTACGGCAA 
     5881 GCTGGAGATC AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTGGT 
     5941 GACCACCCTC TGCTACGGCA TCCAGTGCTT CGCCCGCTAC CCCGAGCACA TGAAGATGAA 
     6001 CGACTTCTTC AAGAGCGCCA TGCCCGAGGG CTACATCCAG GAGCGCACCA TCCAGTTCCA 
     6061 GGACGACGGC AAGTACAAGA CCCGCGGCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA 
     6121 CCGCATCGAG CTGAAGGGCA AGGACTTCAA GGAGGACGGC AACATCCTGG GCCACAAGCT 
     6181 GGAGTACAGC TTCAACAGCC ACAACGTGTA CATCCGCCCC GACAAGGCCA ACAACGGCCT 
     6241 GGAGGCTAAC TTCAAGACCC GCCACAACAT CGAGGGCGGC GGCGTGCAGC TGGCCGACCA 
     6301 CTACCAGACC AACGTGCCCC TGGGCGACGG CCCCGTGCTG ATCCCCATCA ACCACTACCT 
     6361 GAGCACTCAG ACCAAGATCA GCAAGGACCG CAACGAGGCC CGCGACCACA TGGTGCTCCT 
     6421 GGAGTCCTTC AGCGCCTGCT GCCACACCCA CGGCATGGAC GAGCTGTACA GGTCCGGACT 
     6481 CAGATAAGTT TAAACGGCCG GCCGCGGTCT GTACAAGTAG GATTCGTCGA GGGACCTAAT 
     6541 AACTTCGTAT AGCATACATT ATACGAAGTT ATACATGTTT AAGGGTTCCG GTTCCACTAG 
     6601 GTACAATTCG ATATCAAGCT TATCGATAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA 
     6661 TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG 
     6721 CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC 
     6781 TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC 
     6841 ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT 
     6901 TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT 
     6961 GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG 
     7021 AAATCATCGT CCTTTCCTTG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG 
     7081 TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG 
     7141 CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT 
     7201 TGGGCCGCCT CCCCGCATCG ATACCGTCGA CCTCGATCGA GACCTAGAAA AACATGGAGC 
     7261 AATCACAAGT AGCAATACAG CAGCTACCAA TGCTGATTGT GCCTGGCTAG AAGCACAAGA 
     7321 GGAGGAGGAG GTGGGTTTTC CAGTCACACC TCAGGTACCT TTAAGACCAA TGACTTACAA 
     7381 GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG GGACTGGAAG GGCTAATTCA 
     7441 CTCCCAACGA AGACAAGATA TCCTTGATCT GTGGATCTAC CACACACAAG GCTACTTCCC 
     7501 TGATTGGCAG AACTACACAC CAGGGCCAGG GATCAGATAT CCACTGACCT TTGGATGGTG 
     7561 CTACAAGCTA GTACCAGTTG AGCAAGAGAA GGTAGAAGAA GCCAATGAAG GAGAGAACAC 
     7621 CCGCTTGTTA CACCCTGTGA GCCTGCATGG GATGGATGAC CCGGAGAGAG AAGTATTAGA 
     7681 GTGGAGGTTT GACAGCCGCC TAGCATTTCA TCACATGGCC CGAGAGCTGC ATCCGGACTG 
     7741 TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA 
     7801 CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT 
     7861 GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC 
     7921 TAGCAGCATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     7981 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     8041 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     8101 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     8161 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     8221 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     8281 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     8341 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     8401 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     8461 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     8521 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     8581 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     8641 TTTGGTCATG ATTACGCCCC GCCCTGCCAC TCATCGCAGT ACTGTTGTAA TTCATTAAGC 
     8701 ATTCTGCCGA CATGGAAGCC ATCACAAACG GCATGATGAA CCTGAATCGC CAGCGGCATC 
     8761 AGCACCTTGT CGCCTTGCGT ATAATATTTG CCCATGGTGA AAACGGGGGC GAAGAAGTTG 
     8821 TCCATATTGG CCACGTTTAA ATCAAAACTG GTGAAACTCA CCCAGGGATT GGCTGAGACG 
     8881 AAAAACATAT TCTCAATAAA CCCTTTAGGG AAATAGGCCA GGTTTTCACC GTAACACGCC 
     8941 ACATCTTGCG AATATATGTG TAGAAACTGC CGGAAATCGT CGTGGTATTC ACTCCAGAGC 
     9001 GATGAAAACG TTTCAGTTTG CTCATGGAAA ACGGTGTAAC AAGGGTGAAC ACTATCCCAT 
     9061 ATCACCAGCT CACCGTCTTT CATTGCCATA CGGAACTCCG GATGAGCATT CATCAGGCGG 
     9121 GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA 
     9181 AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC ATTGAGCAAC TGACTGAAAT 
     9241 GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA CGGTGGTATA TCCAGTGATT 
     9301 TTTTTCTCCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     9361 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     9421 TTTCCCCGAA AAGTGCCACC TGAC
//