LOCUS       RC209509                8502 bp    DNA     circular  SYN 
DEFINITION  SF3B3 (Myc-DDK-tagged)-Human splicing factor 3b, subunit 3, 130kDa (SF3B3)
ACCESSION   NM_012426
VERSION     NM_012426.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4659..4750 
                     /label="Myc-DDK"  
     misc_feature    4780..5365 
                     /label="PolyA signal"  
     misc_feature    4837..4856 
                     /label="Restriction Enzyme"  
     misc_feature    5641..6313 
                     /label="ColE1"  
     misc_feature    6743..7537 
                     /label="Kan/Neo"  
     misc_feature    7572..7874 
                     /label="SV40 ori"  
     misc_feature    7960..8437 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4641..4647 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4641 
                     /label="SF3B3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTTCTGTAC AACTTAACCT TGCAGAGAGC CACTGGCATC AGCTTTGCCA 
     1081 TTCATGGAAA CTTTTCTGGA ACCAAACAAC AAGAAATTGT TGTTTCCCGT GGGAAGATCT 
     1141 TGGAGCTGCT TCGCCCAGAC CCCAACACTG GCAAAGTACA TACCCTACTC ACTGTGGAAG 
     1201 TATTCGGTGT TATCCGGTCA CTCATGGCCT TTAGGCTGAC AGGTGGCACC AAAGACTACA 
     1261 TTGTAGTTGG CAGTGACTCT GGTCGAATTG TTATTTTGGA ATACCAGCCA TCTAAGAATA 
     1321 TGTTTGAGAA GATTCACCAA GAAACCTTTG GCAAGAGTGG ATGCCGTCGC ATCGTTCCTG 
     1381 GCCAGTTCTT AGCTGTGGAT CCCAAAGGGC GAGCCGTTAT GATTAGTGCC ATTGAGAAAC 
     1441 AGAAATTGGT GTATATTTTG AACAGAGATG CTGCAGCCCG ACTTACCATT TCATCTCCCC 
     1501 TGGAAGCCCA CAAAGCAAAC ACTTTAGTGT ATCATGTAGT TGGAGTAGAT GTCGGATTTG 
     1561 AAAATCCAAT GTTTGCTTGT CTGGAAATGG ATTATGAGGA AGCAGACAAT GATCCAACAG 
     1621 GGGAAGCAGC AGCTAATACC CAGCAGACAC TTACTTTCTA TGAGCTAGAC CTTGGTTTAA 
     1681 ATCATGTGGT CCGAAAATAC AGTGAACCTT TGGAGGAACA CGGCAACTTC CTTATTACAG 
     1741 TTCCAGGAGG GTCAGATGGT CCAAGTGGAG TACTGATCTG CTCTGAAAAC TATATTACTT 
     1801 ACAAGAACTT TGGTGACCAG CCAGATATCC GCTGTCCAAT TCCCAGGAGG CGGAATGACC 
     1861 TGGATGACCC TGAAAGAGGA ATGATTTTTG TCTGCTCTGC AACCCATAAA ACCAAATCGA 
     1921 TGTTCTTCTC TTTGGCTCAA ACTGAGCAGG GAGATATCTT TAAGATCACT TTGGAGACAG 
     1981 ATGAAGATAT GGTTACTGAG ATCCGGCTCA AATATTTTGA TACTGTACCC GTTGCTGCTG 
     2041 CCATGTGTGT GCTTAAAACA GGGTTCCTTT TTGTAGCATC AGAATTTGGA AACCATTACT 
     2101 TATATCAAAT TGCACATCTT GGAGATGATG ATGAAGAACC TGAGTTTTCA TCAGCCATGC 
     2161 CTCTGGAAGA AGGAGACACA TTCTTTTTTC AGCCAAGACC ACTTAAAAAC CTTGTGCTGG 
     2221 TTGATGAGTT GGACAGCCTC TCTCCCATTC TGTTTTGCCA GATAGCTGAT CTGGCCAATG 
     2281 AAGATACTCC ACAGTTGTAT GTGGCCTGTG GTAGGGGACC CCGATCATCT CTGAGAGTCC 
     2341 TAAGACATGG ACTTGAGGTG TCAGAAATGG CTGTTTCTGA GCTACCTGGT AACCCCAACG 
     2401 CTGTCTGGAC AGTGCGTCGA CACATTGAAG ATGAGTTTGA TGCCTACATT ATTGTGTCTT 
     2461 TCGTGAATGC CACCCTAGTG TTGTCCATTG GAGAAACTGT AGAAGAAGTG ACTGACTCTG 
     2521 GGTTCCTGGG GACCACCCCG ACCTTGTCCT GCTCCTTATT AGGAGATGAT GCCTTGGTGC 
     2581 AGGTCTATCC AGATGGCATT CGGCACATAC GAGCAGACAA GAGAGTCAAT GAGTGGAAGA 
     2641 CCCCTGGAAA GAAAACAATT GTGAAGTGTG CAGTGAACCA GCGACAAGTG GTGATTGCCC 
     2701 TGACAGGAGG AGAGCTGGTC TATTTCGAGA TGGATCCTTC AGGACAGCTG AATGAGTACA 
     2761 CAGAACGGAA GGAGATGTCA GCAGATGTGG TGTGCATGAG TCTGGCCAAT GTACCCCCTG 
     2821 GAGAGCAGCG GTCTCGCTTC CTGGCTGTGG GGCTTGTGGA CAACACTGTC AGAATCATCT 
     2881 CCCTGGATCC CTCAGACTGT TTGCAACCTC TAAGCATGCA GGCTCTCCCA GCCCAGCCTG 
     2941 AGTCCTTGTG TATCGTGGAA ATGGGTGGGA CTGAGAAGCA GGATGAGCTG GGTGAGAGGG 
     3001 GCTCGATTGG CTTCCTATAC CTGAATATTG GGCTACAGAA CGGTGTGCTG CTGAGGACTG 
     3061 TCTTGGACCC TGTCACTGGG GATTTGTCTG ATACTCGCAC TCGGTACCTG GGGTCCCGTC 
     3121 CTGTGAAGCT CTTCCGAGTC CGAATGCAAG GCCAGGAGGC AGTATTGGCC ATGTCAAGCC 
     3181 GCTCATGGTT GAGCTATTCT TACCAATCTC GCTTCCATCT CACCCCACTG TCTTACGAGA 
     3241 CACTGGAATT TGCATCGGGT TTTGCCTCGG AACAGTGTCC CGAGGGCATT GTGGCCATCT 
     3301 CCACCAACAC CCTACGGATT TTGGCATTAG AGAAGCTCGG TGCTGTCTTC AATCAAGTAG 
     3361 CCTTCCCACT GCAGTACACA CCCAGGAAAT TTGTCATCCA CCCTGAGAGT AACAACCTTA 
     3421 TTATCATTGA AACGGACCAC AATGCCTACA CTGAGGCCAC GAAAGCTCAG AGAAAGCAGC 
     3481 AGATGGCAGA GGAAATGGTG GAAGCAGCAG GGGAGGATGA GCGGGAGCTG GCCGCAGAGA 
     3541 TGGCAGCAGC ATTCCTCAAT GAAAACCTCC CTGAATCCAT CTTTGGAGCT CCCAAGGCTG 
     3601 GCAATGGGCA GTGGGCCTCT GTGATCCGAG TGATGAATCC CATTCAAGGG AACACACTGG 
     3661 ACCTTGTCCA GCTGGAACAG AATGAGGCAG CTTTTAGTGT GGCTGTGTGC AGGTTTTCCA 
     3721 ACACTGGTGA AGACTGGTAT GTGCTGGTGG GTGTGGCCAA GGACCTGATA CTAAACCCCC 
     3781 GATCTGTGGC AGGGGGCTTC GTCTATACTT ACAAGCTTGT GAACAATGGG GAAAAACTGG 
     3841 AGTTTTTGCA CAAGACTCCT GTGGAAGAGG TCCCTGCTGC TATTGCCCCA TTCCAGGGGA 
     3901 GGGTGTTGAT TGGTGTGGGG AAGCTGTTGC GTGTCTATGA CCTGGGAAAG AAGAAGTTAC 
     3961 TCCGAAAATG TGAGAATAAG CATATTGCCA ATTATATCTC TGGGATCCAG ACTATCGGAC 
     4021 ATAGGGTAAT TGTATCTGAT GTCCAAGAAA GTTTCATCTG GGTTCGCTAC AAGCGTAATG 
     4081 AAAACCAGCT TATCATCTTT GCTGATGATA CCTACCCCCG ATGGGTCACT ACAGCCAGCC 
     4141 TCCTGGACTA TGACACTGTG GCTGGGGCAG ACAAGTTTGG CAACATATGT GTGGTGAGGC 
     4201 TCCCACCTAA CACCAATGAT GAAGTAGATG AGGATCCTAC AGGAAACAAA GCCCTGTGGG 
     4261 ACCGTGGCTT GCTCAATGGG GCCTCCCAGA AGGCAGAGGT GATCATGAAC TACCATGTCG 
     4321 GGGAGACGGT GCTGTCCTTG CAGAAGACCA CGCTGATCCC TGGAGGCTCA GAATCACTTG 
     4381 TCTATACCAC CTTGTCTGGA GGAATTGGCA TCCTTGTGCC ATTCACGTCC CATGAGGACC 
     4441 ATGACTTCTT CCAGCATGTG GAAATGCACC TGCGGTCTGA ACATCCCCCT CTCTGTGGGC 
     4501 GGGACCACCT CAGCTTTCGC TCCTACTACT TCCCTGTGAA GAATGTGATT GATGGAGACC 
     4561 TCTGTGAGCA GTTCAATTCC ATGGAACCCA ACAAACAAAA GAACGTCTCT GAAGAACTGG 
     4621 ACCGAACCCC ACCCGAAGTG TCCAAGAAAA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4681 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4741 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4801 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4861 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4921 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4981 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5041 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5101 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5161 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5221 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5281 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5341 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5401 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5461 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5521 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5581 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5641 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5701 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5761 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5821 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5881 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5941 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6001 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6061 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6121 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6181 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6241 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6301 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6361 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6421 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6481 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6541 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6601 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6661 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6721 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6781 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6841 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6901 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6961 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7021 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7081 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7141 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7201 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7261 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7321 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7381 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7441 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7501 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7561 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7621 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7681 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7741 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7801 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7861 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7921 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7981 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8041 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8101 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8161 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8221 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8281 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8341 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8401 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8461 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//