LOCUS       RC209476                7947 bp    DNA     circular  SYN 
DEFINITION  COL6A2 (Myc-DDK-tagged)-Human collagen, type VI, alpha 2 (COL6A2), transcript variant 2C2
ACCESSION   NM_001849
VERSION     NM_001849.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4102..4193 
                     /label="Myc-DDK"  
     misc_feature    4223..4808 
                     /label="PolyA signal"  
     misc_feature    4280..4299 
                     /label="Restriction Enzyme"  
     misc_feature    5084..5756 
                     /label="ColE1"  
     misc_feature    6186..6980 
                     /label="Kan/Neo"  
     misc_feature    7015..7317 
                     /label="SV40 ori"  
     misc_feature    7403..7880 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4077..4084 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4077 
                     /label="COL6A2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTCCAGGGC ACCTGCTCCG TGCTCCTGCT CTGGGGAATC CTGGGGGCCA 
     1081 TCCAGGCCCA GCAGCAGGAG GTCATCTCGC CGGACACTAC CGAGAGAAAC AACAACTGCC 
     1141 CAGAGAAGAC CGACTGCCCC ATCCACGTGT ACTTCGTGCT GGACACCTCG GAGAGCGTCA 
     1201 CCATGCAGTC CCCCACGGAC ATCCTGCTCT TCCACATGAA GCAGTTCGTG CCGCAGTTCA 
     1261 TCAGCCAGCT GCAGAACGAG TTCTACCTGG ACCAGGTGGC GCTGAGCTGG CGCTACGGCG 
     1321 GCCTGCACTT CTCTGACCAG GTGGAGGTGT TCAGCCCACC GGGCAGCGAC CGGGCCTCCT 
     1381 TCATCAAGAA CCTGCAGGGC ATCAGCTCCT TCCGCCGCGG CACCTTCACC GACTGCGCGC 
     1441 TGGCCAACAT GACGGAGCAG ATCCGGCAGG ACCGCAGCAA GGGCACCGTC CACTTCGCCG 
     1501 TGGTCATCAC CGACGGCCAC GTCACCGGCA GCCCCTGCGG GGGCATCAAG CTGCAGGCCG 
     1561 AGCGGGCCCG CGAGGAGGGC ATCCGGCTCT TCGCCGTGGC CCCCAACCAG AACCTGAAGG 
     1621 AGCAGGGCCT GCGGGACATC GCCAGCACGC CGCACGAGCT CTACCGCAAC GACTACGCCA 
     1681 CCATGCTGCC CGACTCCACC GAGATCGACC AGGACACCAT CAACCGCATC ATCAAGGTCA 
     1741 TGAAACACGA AGCCTACGGA GAGTGCTACA AGGTGAGCTG CCTGGAAATC CCTGGGCCCT 
     1801 CTGGCCCCAA GGGCTACCGT GGACAGAAGG GTGCCAAGGG CAACATGGGT GAGCCGGGAG 
     1861 AGCCTGGCCA GAAGGGAAGA CAGGGAGACC CGGGCATCGA AGGCCCCATT GGATTCCCAG 
     1921 GACCCAAGGG CGTTCCTGGC TTCAAAGGAG AGAAGGGTGA ATTTGGAGCC GACGGTCGCA 
     1981 AGGGGGCCCC TGGCCTGGCT GGCAAGAACG GGACCGATGG ACAGAAGGGC AAGCTGGGGC 
     2041 GCATCGGACC TCCTGGCTGC AAGGGAGACC CTGGAAACCG GGGCCCCGAC GGTTACCCGG 
     2101 GGGAAGCAGG GAGTCCAGGG GAGCGAGGAG ACCAAGGCGG CAAGGGGGAC CCTGGCCGCC 
     2161 CAGGACGCAG AGGGCCCCCG GGAGAAATCG GGGCCAAGGG AAGCAAGGGG TATCAAGGCA 
     2221 ACAATGGAGC CCCAGGAAGT CCTGGTGTGA AAGGAGCCAA GGGCGGGCCT GGGCCCCGCG 
     2281 GACCCAAAGG CGAGCCGGGG CGCAGGGGAG ACCCCGGCAC CAAGGGCAGC CCAGGCAGCG 
     2341 ATGGCCCCAA GGGGGAGAAG GGGGACCCTG GCCCTGAGGG GCCCCGCGGC CTGGCTGGAG 
     2401 AGGTTGGCAA CAAAGGAGCC AAGGGAGACC GAGGCTTGCC TGGACCCAGA GGCCCCCAGG 
     2461 GAGCTCTTGG GGAGCCCGGA AAGCAGGGAT CTCGGGGAGA CCCCGGTGAT GCAGGACCCC 
     2521 GTGGAGACTC AGGACAGCCA GGCCCCAAGG GAGACCCCGG CAGGCCTGGA TTCAGCTACC 
     2581 CAGGACCCCG AGGAGCACCC GGAGAAAAAG GCGAGCCCGG CCCACGCGGC CCCGAGGGAG 
     2641 GCCGAGGCGA CTTTGGCTTG AAAGGAGAAC CTGGGAGGAA AGGAGAGAAA GGAGAGCCTG 
     2701 CGGATCCTGG TCCCCCTGGT GAGCCAGGCC CTCGGGGGCC AAGAGGAGTC CCAGGACCCG 
     2761 AGGGTGAGCC CGGCCCCCCT GGAGACCCCG GTCTCACGGA GTGTGACGTC ATGACCTACG 
     2821 TGAGGGAGAC CTGCGGGTGC TGCGACTGTG AGAAGCGCTG TGGCGCCCTG GACGTGGTCT 
     2881 TCGTCATCGA CAGCTCCGAG AGCATTGGGT ACACCAACTT CACACTGGAG AAGAACTTCG 
     2941 TCATCAACGT GGTCAACAGG CTGGGTGCCA TCGCTAAGGA CCCCAAGTCC GAGACAGGGA 
     3001 CGCGTGTGGG CGTGGTGCAG TACAGCCACG AGGGCACCTT TGAGGCCATC CAGCTGGACG 
     3061 ACGAACGTAT CGACTCCCTG TCGAGCTTCA AGGAGGCTGT CAAGAACCTC GAGTGGATTG 
     3121 CGGGCGGCAC CTGGACACCC TCAGCCCTCA AGTTTGCCTA CGACCGCCTC ATCAAGGAGA 
     3181 GCCGGCGCCA GAAGACACGT GTGTTTGCGG TGGTCATCAC GGACGGGCGC CACGACCCTC 
     3241 GGGACGATGA CCTCAACTTG CGGGCGCTGT GCGACCGCGA CGTCACAGTG ACGGCCATCG 
     3301 GCATCGGGGA CATGTTCCAC GAGAAGCACG AGAGTGAAAA CCTCTACTCC ATCGCCTGCG 
     3361 ACAAGCCACA GCAGGTGCGC AACATGACGC TGTTCTCCGA CCTGGTCGCT GAGAAGTTCA 
     3421 TCGATGACAT GGAGGACGTC CTCTGCCCGG ACCCTCAGAT CGTGTGCCCA GACCTTCCCT 
     3481 GCCAAACAGA GCTGTCCGTG GCACAGTGCA CGCAGCGGCC CGTGGACATC GTCTTCCTGC 
     3541 TGGACGGCTC CGAGCGGCTG GGTGAGCAGA ACTTCCACAA GGCCCGGCGC TTCGTGGAGC 
     3601 AGGTGGCGCG GCGGCTGACG CTGGCCCGGA GGGACGACGA CCCTCTCAAC GCACGCGTGG 
     3661 CGCTGCTGCA GTTTGGTGGC CCCGGCGAGC AGCAGGTGGC CTTCCCGCTG AGCCACAACC 
     3721 TCACGGCCAT CCACGAGGCG CTGGAGACCA CGCAATACCT GAACTCCTTC TCGCACGTGG 
     3781 GCGCAGGCGT GGTGCACGCC ATCAATGCCA TCGTGCGCAG CCCGCGTGGC GGGGCCCGGA 
     3841 GGCACGCAGA GCTGTCCTTC GTGTTCCTCA CGGACGGCGT CACGGGCAAC GACAGTCTGC 
     3901 ACGAGTCGGC GCACTCCATG CGCAAGCAGA ACGTGGTACC CACCGTGCTG GCCTTGGGCA 
     3961 GCGACGTGGA CATGGACGTG CTCACCACGC TCAGCCTGGG TGACCGTGCC GCCGTGTTCC 
     4021 ACGAGAAGGA CTATGACAGC CTGGCGCAAC CCGGCTTCTT CGACCGCTTC ATCCGCTGGA 
     4081 TCTGCAGCGG ACCGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4141 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4201 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4261 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4321 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4381 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4441 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4501 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4561 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4621 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4681 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4741 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4801 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4861 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4921 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     4981 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5041 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5101 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5161 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5221 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5281 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5341 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5401 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5461 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5521 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5581 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5641 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5701 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5761 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5821 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     5881 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     5941 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6001 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6061 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6121 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6181 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6241 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6301 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6361 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6421 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6481 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6541 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6601 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6661 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6721 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6781 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6841 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6901 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     6961 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7021 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7081 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7141 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7201 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7261 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7321 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7381 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7441 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7501 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7561 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7621 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7681 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7741 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7801 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7861 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7921 ATTTAACAAA AATTTAACGC GAATTTT
//