LOCUS       RC209308L1                9330 bp    DNA     circular  SYN 
DEFINITION  RNF111 (Myc-DDK-tagged)-Human ring finger protein 111 (RNF111)
ACCESSION   NM_017610
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7621..7801 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8529..9188 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7960..8528 
                     /label="ColE1"  
     misc_feature    6496..6516 
                     /label="LR50 primer"  
     misc_feature    6293..6364 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6505..7096 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6275..6281 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6275 
                     /label="RNF111"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GTCTCAATGG ACTCCTGAAT ATAAGGAGCT 
     3361 CTACACCTTA AAAGTGGATA TGAAGAGTGA GATTCCTTCT GATGCACCAA AGACACAGGA 
     3421 GAGTCTGAAA GGGATCCTTT TGCATCCAGA GCCCATTGGG GCAGCCAAAA GTTTTCCTGC 
     3481 AGGAGTTGAG ATGATTAATA GTAAAGTGGG GAATGAATTC TCTCACCTGT GTGATGATTC 
     3541 TCAAAAGCAA GAGAAGGAAA TGAATGGTAA CCAGCAAGAA CAAGAAAAAA GTCTCGTTGT 
     3601 GAGGAAAAAA CGCAAAAGCC AGCAGGCTGG CCCTTCGTAT GTGCAGAATT GTGTTAAAGA 
     3661 AAACCAGGGA ATATTAGGAC TGAGGCAACA CCTAGGGACA CCAAGTGATG AAGATAATGA 
     3721 TTCCTCTTTT AGTGATTGTC TTTCTTCTCC TTCATCTAGT CTGCATTTTG GGGATTCTGA 
     3781 TACTGTGACT TCAGATGAGG ATAAAGAAGT CTCTGTAAGA CATTCCCAGA CCATTTTGAA 
     3841 TGCTAAAAGT AGAAGCCATA GTGCACGGTC TCATAAGTGG CCTCGGACTG AGACAGAATC 
     3901 TGTATCGGGA TTGTTAATGA AAAGACCCTG TTTACATGGC AGTTCGTTAC GGAGACTTCC 
     3961 ATGCAGAAAG AGATTTGTAA AAAATAATTC CTCACAGAGG ACACAGAAAC AAAAAGAGAG 
     4021 GATATTAATG CAGAGGAAGA AACGAGAAGT GTTAGCTCGA AGAAAATATG CCTTGCTACC 
     4081 TAGTTCTAGT AGTTCCAGTG AGAATGACCT CAGCAGTGAA TCCTCTTCTA GCTCATCAAC 
     4141 TGAAGGAGAA GAAGATTTGT TTGTTTCTGC CAGTGAAAAC CACCAAAACA ATCCAGCTGT 
     4201 TCCCTCAGGA AGTATTGATG AAGATGTTGT GGTGATAGAA GCTTCCTCCA CTCCCCAGGT 
     4261 TACTGCCAAT GAAGAAATTA ATGTTACCTC AACTGACAGT GAAGTGGAGA TTGTAACAGT 
     4321 TGGAGAAAGC TATCGGTCTC GTTCAACCCT TGGACACTCC AGATCTCATT GGAGCCAGGG 
     4381 TTCCAGTTCT CATGCAAGTC GGCCACAGGA GCCACGGAAC CGCAGTAGGA TTTCTACTGT 
     4441 TATACAGCCC TTGAGGCAGA ATGCAGCAGA AGTTGTGGAC CTTACCGTTG ATGAAGATGA 
     4501 ACCTACTGTA GTACCAACCA CTTCTGCAAG AATGGAATCA CAAGCTACTA GCGCTTCCAT 
     4561 TAACAATTCA AATCCATCTA CCTCTGAGCA GGCCTCTGAT ACTGCTTCAG CTGTCACCAG 
     4621 TAGCCAACCT TCCACAGTGT CAGAGACTTC AGCTACTCTT ACAAGCAATA GTACCACTGG 
     4681 CACTTCTATA GGAGATGACT CAAGGAGAAC TACATCTAGT GCTGTAACGG AAACTGGCCC 
     4741 TCCTGCAATG CCAAGGTTAC CTTCCTGCTG TCCCCAGCAC TCACCATGTG GAGGGTCGTC 
     4801 ACAGAACCAC CATGCATTAG GACATCCTCA TACAAGTTGC TTTCAGCAGC ATGGTCACCA 
     4861 TTTTCAACAT CATCACCACC ACCACCATAC TCCCCACCCA GCTGTCCCAG TTTCTCCTTC 
     4921 CTTTAGTGAT CCTGCTTGCC CTGTGGAAAG ACCTCCACAA GTACAAGCAC CTTGTGGAGC 
     4981 AAATAGTAGT TCTGGTACCA GCTATCATGA ACAGGCATTG CCAGTGGACC TGAGCAACAG 
     5041 TGGTATCAGA AGTCATGGAA GTGGCAGTTT TCATGGAGCA TCTGCATTTG ACCCCTGCTG 
     5101 CCCTGTTTCT TCCTCCCGAG CTGCAATCTT TGGCCATCAG GCCGCTGCTG CTGCCCCAAG 
     5161 TCAACCTTTA TCATCAATAG ATGGCTATGG ATCAAGCATG GTTGCGCAGC CCCAGCCCCA 
     5221 GCCCCCTCCA CAGCCCTCTC TCTCATCATG TCGACATTAC ATGCCACCCC CTTATGCCTC 
     5281 TTTGACAAGG CCACTTCATC ATCAAGCTTC TGCCTGCCCG CATTCTCATG GAAACCCCCC 
     5341 TCCTCAGACT CAGCCTCCGC CTCAAGTGGA TTATGTTATT CCTCATCCTG TACATGCTTT 
     5401 CCATTCTCAA ATATCTTCTC ATGCAACATC TCATCCTGTG GCACCCCCAC CACCAACTCA 
     5461 CTTAGCCAGT ACAGCTGCAC CAATCCCTCA GCATCTTCCT CCTACACACC AGCCAATTTC 
     5521 GCACCATATT CCAGCCACAG CACCTCCAGC ACAGAGACTG CATCCTCATG AAGTGATGCA 
     5581 GAGGATGGAA GTTCAAAGGA GGAGGATGAT GCAGCATCCA ACGCGGGCAC ATGAACGCCC 
     5641 CCCACCCCAT CCACATAGGA TGCACCCAAA CTATGGTCAT GGGCATCATA TTCATGTGCC 
     5701 TCAGACTATG TCCTCACATC CTCGACAGGC TCCAGAGAGG TCTGCCTGGG AACTGGGAAT 
     5761 TGAAGCTGGA GTGACTGCAG CTACTTATAC ACCTGGTGCA TTGCATCCTC ACTTGGCCCA 
     5821 TTATCACGCA CCTCCTCGAC TTCATCACTT ACAATTAGGA GCTCTTCCTT TAATGGTTCC 
     5881 TGATATGGCA GGCTATCCTC ACATCCGTTA CATTTCATCA GGATTGGATG GAACATCATT 
     5941 CAGAGGTCCT TTCAGGGGCA ATTTTGAGGA ACTGATTCAT TTGGAAGAAA GATTAGGCAA 
     6001 TGTCAATCGT GGAGCATCCC AGGGGACAAT TGAAAGATGT ACATATCCAC ATAAATACAA 
     6061 AAAGAGGAAA CTGCACTGCA AACAAGATGG GGAAGAAGGG ACTGAGGAAG ACACAGAGGA 
     6121 AAAATGTACT ATCTGTTTGT CTATTTTAGA GGAAGGTGAA GATGTGAGAC GTCTTCCATG 
     6181 TATGCACCTT TTCCACCAAG TGTGTGTTGA CCAATGGTTG ATTACCAATA AGAAGTGCCC 
     6241 CATATGCAGA GTGGACATTG AGGCCCAGCT GCCAAGTGAA AGTACGCGTA CGCGGCCGCT 
     6301 CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA 
     6361 TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCTGTAC AAGTAGGATT CGTCGAGGGA 
     6421 CCTAATAACT TCGTATAGCA TACATTATAC GAAGTTATAC ATGTTTAAGG GTTCCGGTTC 
     6481 CACTAGGTAC AATTCGATAT CAAGCTTATC GATAATCAAC CTCTGGATTA CAAAATTTGT 
     6541 GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT 
     6601 TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT 
     6661 AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG 
     6721 GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG 
     6781 CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC 
     6841 TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG 
     6901 TCGGGGAAAT CATCGTCCTT TCCTTGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC 
     6961 GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC 
     7021 CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC GAGTCGGATC 
     7081 TCCCTTTGGG CCGCCTCCCC GCATCGATAC CGTCGACCTC GATCGAGACC TAGAAAAACA 
     7141 TGGAGCAATC ACAAGTAGCA ATACAGCAGC TACCAATGCT GATTGTGCCT GGCTAGAAGC 
     7201 ACAAGAGGAG GAGGAGGTGG GTTTTCCAGT CACACCTCAG GTACCTTTAA GACCAATGAC 
     7261 TTACAAGGCA GCTGTAGATC TTAGCCACTT TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT 
     7321 AATTCACTCC CAACGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA CACAAGGCTA 
     7381 CTTCCCTGAT TGGCAGAACT ACACACCAGG GCCAGGGATC AGATATCCAC TGACCTTTGG 
     7441 ATGGTGCTAC AAGCTAGTAC CAGTTGAGCA AGAGAAGGTA GAAGAAGCCA ATGAAGGAGA 
     7501 GAACACCCGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG AGAGAGAAGT 
     7561 ATTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC ATGGCCCGAG AGCTGCATCC 
     7621 GGACTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT CTGGCTAACT 
     7681 AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC 
     7741 CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA 
     7801 AATCTCTAGC AGCATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     7861 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     7921 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     7981 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     8041 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     8101 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     8161 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     8221 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     8281 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     8341 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     8401 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     8461 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     8521 AGGGATTTTG GTCATGATTA CGCCCCGCCC TGCCACTCAT CGCAGTACTG TTGTAATTCA 
     8581 TTAAGCATTC TGCCGACATG GAAGCCATCA CAAACGGCAT GATGAACCTG AATCGCCAGC 
     8641 GGCATCAGCA CCTTGTCGCC TTGCGTATAA TATTTGCCCA TGGTGAAAAC GGGGGCGAAG 
     8701 AAGTTGTCCA TATTGGCCAC GTTTAAATCA AAACTGGTGA AACTCACCCA GGGATTGGCT 
     8761 GAGACGAAAA ACATATTCTC AATAAACCCT TTAGGGAAAT AGGCCAGGTT TTCACCGTAA 
     8821 CACGCCACAT CTTGCGAATA TATGTGTAGA AACTGCCGGA AATCGTCGTG GTATTCACTC 
     8881 CAGAGCGATG AAAACGTTTC AGTTTGCTCA TGGAAAACGG TGTAACAAGG GTGAACACTA 
     8941 TCCCATATCA CCAGCTCACC GTCTTTCATT GCCATACGGA ACTCCGGATG AGCATTCATC 
     9001 AGGCGGGCAA GAATGTGAAT AAAGGCCGGA TAAAACTTGT GCTTATTTTT CTTTACGGTC 
     9061 TTTAAAAAGG CCGTAATATC CAGCTGAACG GTCTGGTTAT AGGTACATTG AGCAACTGAC 
     9121 TGAAATGCCT CAAAATGTTC TTTACGATGC CATTGGGATA TATCAACGGT GGTATATCCA 
     9181 GTGATTTTTT TCTCCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     9241 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTCCCG 
     9301 CGCACATTTC CCCGAAAAGT GCCACCTGAC 
//