LOCUS       RC209258                8715 bp    DNA     circular  SYN 
DEFINITION  NPC1 (Myc-DDK-tagged)-Human Niemann-Pick disease, type C1 (NPC1)
ACCESSION   NM_000271
VERSION     NM_000271.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4872..4963 
                     /label="Myc-DDK"  
     misc_feature    4993..5578 
                     /label="PolyA signal"  
     misc_feature    5050..5069 
                     /label="Restriction Enzyme"  
     misc_feature    5854..6526 
                     /label="ColE1"  
     misc_feature    6956..7750 
                     /label="Kan/Neo"  
     misc_feature    7785..8087 
                     /label="SV40 ori"  
     misc_feature    8173..8650 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4854..4860 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4854 
                     /label="NPC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCGCTCGC GGCCTGGCCC TTGGCCTCCT CCTGCTGCTA CTGTGTCCAG 
     1081 CGCAGGTGTT TTCACAGTCC TGTGTTTGGT ATGGAGAGTG TGGAATTGCA TATGGGGACA 
     1141 AGAGGTACAA TTGCGAATAT TCTGGCCCAC CAAAACCATT GCCAAAGGAT GGATATGACT 
     1201 TAGTGCAGGA ACTCTGTCCA GGATTCTTCT TTGGCAATGT CAGTCTCTGT TGTGATGTTC 
     1261 GGCAGCTTCA GACACTAAAA GACAACCTGC AGCTGCCTCT ACAGTTTCTG TCCAGATGTC 
     1321 CATCCTGTTT TTATAACCTA CTGAACCTGT TTTGTGAGCT GACATGTAGC CCTCGACAGA 
     1381 GTCAGTTTTT GAATGTTACA GCTACTGAAG ATTATGTTGA TCCTGTTACA AACCAGACGA 
     1441 AAACAAATGT GAAAGAGTTA CAATACTACG TCGGACAGAG TTTTGCCAAT GCAATGTACA 
     1501 ATGCCTGCCG GGATGTGGAG GCCCCCTCAA GTAATGACAA GGCCCTGGGA CTCCTGTGTG 
     1561 GGAAGGACGC TGACGCCTGT AATGCCACCA ACTGGATTGA ATACATGTTC AATAAGGACA 
     1621 ATGGACAGGC ACCTTTTACC ATCACTCCTG TGTTTTCAGA TTTTCCAGTC CATGGGATGG 
     1681 AGCCCATGAA CAATGCCACC AAAGGCTGTG ACGAGTCTGT GGATGAGGTC ACAGCACCAT 
     1741 GTAGCTGCCA AGACTGCTCT ATTGTCTGTG GCCCCAAGCC CCAGCCCCCA CCTCCTCCTG 
     1801 CTCCCTGGAC GATCCTTGGC TTGGACGCCA TGTATGTCAT CATGTGGATC ACCTACATGG 
     1861 CGTTTTTGCT TGTGTTTTTT GGAGCATTTT TTGCAGTGTG GTGCTACAGA AAACGGTATT 
     1921 TTGTCTCCGA GTACACTCCC ATCGATAGCA ATATAGCTTT TTCTGTTAAT GCAAGTGACA 
     1981 AAGGAGAGGC GTCCTGCTGT GACCCTGTCA GCGCAGCATT TGAGGGCTGC TTGAGGCGGC 
     2041 TGTTCACACG CTGGGGGTCT TTCTGCGTCC GAAACCCTGG CTGTGTCATT TTCTTCTCGC 
     2101 TGGTCTTCAT TACTGCGTGT TCGTCAGGCC TGGTGTTTGT CCGGGTCACA ACCAATCCAG 
     2161 TTGACCTCTG GTCAGCCCCC AGCAGCCAGG CTCGCCTGGA AAAAGAGTAC TTTGACCAGC 
     2221 ACTTTGGGCC TTTCTTCCGG ACGGAGCAGC TCATCATCCG GGCCCCTCTC ACTGACAAAC 
     2281 ACATTTACCA GCCATACCCT TCGGGAGCTG ATGTACCCTT TGGACCTCCG CTTGACATAC 
     2341 AGATACTGCA CCAGGTTCTT GACTTACAAA TAGCCATCGA AAACATTACT GCCTCTTATG 
     2401 ACAATGAGAC TGTGACACTT CAAGACATCT GCTTGGCCCC TCTTTCACCG TATAACACGA 
     2461 ACTGCACCAT TTTGAGTGTG TTAAATTACT TCCAGAACAG CCATTCCGTG CTGGACCACA 
     2521 AGAAAGGGGA CGACTTCTTT GTGTATGCCG ATTACCACAC GCACTTTCTG TACTGCGTAC 
     2581 GGGCTCCTGC CTCTCTGAAT GATACAAGTT TGCTCCATGA CCCTTGTCTG GGTACGTTTG 
     2641 GTGGACCAGT GTTCCCGTGG CTTGTGTTGG GAGGCTATGA TGATCAAAAC TACAATAACG 
     2701 CCACTGCCCT TGTGATTACC TTCCCTGTCA ATAATTACTA TAATGATACA GAGAAGCTCC 
     2761 AGAGGGCCCA GGCCTGGGAA AAAGAGTTTA TTAATTTTGT GAAAAACTAC AAGAATCCCA 
     2821 ATCTGACCAT TTCCTTCACT GCTGAACGAA GTATTGAAGA TGAACTAAAT CGTGAAAGTG 
     2881 ACAGTGATGT CTTCACCGTT GTAATTAGCT ATGCCATCAT GTTTCTATAT ATTTCCCTAG 
     2941 CCTTGGGGCA CATGAAAAGC TGTCGCAGGC TTCTGGTGGA TTCGAAGGTC TCACTAGGCA 
     3001 TCGCGGGCAT CTTGATCGTG CTGAGCTCGG TGGCTTGCTC CTTGGGTGTC TTCAGCTACA 
     3061 TTGGGTTGCC CTTGACCCTC ATTGTGATTG AAGTCATCCC GTTCCTGGTG CTGGCTGTTG 
     3121 GAGTGGACAA CATCTTCATT CTGGTGCAGG CCTACCAGAG AGATGAACGT CTTCAAGGGG 
     3181 AAACCCTGGA TCAGCAGCTG GGCAGGGTCC TAGGAGAAGT GGCTCCCAGT ATGTTCCTGT 
     3241 CATCCTTTTC TGAGACTGTA GCATTTTTCT TAGGAGCATT GTCCGTGATG CCAGCCGTGC 
     3301 ACACCTTCTC TCTCTTTGCG GGATTGGCAG TCTTCATTGA CTTTCTTCTG CAGATTACCT 
     3361 GTTTCGTGAG TCTCTTGGGG TTAGACATTA AACGTCAAGA GAAAAATCGG CTAGACATCT 
     3421 TTTGCTGTGT CAGAGGTGCT GAAGATGGAA CAAGCGTCCA GGCCTCAGAG AGCTGTTTGT 
     3481 TTCGCTTCTT CAAAAACTCC TATTCTCCAC TTCTGCTAAA GGACTGGATG AGACCAATTG 
     3541 TGATAGCAAT ATTTGTGGGT GTTCTGTCAT TCAGCATCGC AGTCCTGAAC AAAGTAGATA 
     3601 TTGGATTGGA TCAGTCTCTT TCGATGCCAG ATGACTCCTA CATGGTGGAT TATTTCAAAT 
     3661 CCATCAGTCA GTACCTGCAT GCGGGTCCGC CTGTGTACTT TGTCCTGGAG GAAGGGCACG 
     3721 ACTACACTTC TTCCAAGGGG CAGAACATGG TGTGCGGCGG CATGGGCTGC AACAATGATT 
     3781 CCCTGGTGCA GCAGATATTT AACGCGGCGC AGCTGGACAA CTATACCCGA ATAGGCTTCG 
     3841 CCCCCTCGTC CTGGATCGAC GATTATTTCG ACTGGGTGAA GCCACAGTCG TCTTGCTGTC 
     3901 GAGTGGACAA TATCACTGAC CAGTTCTGCA ATGCTTCAGT GGTTGACCCT GCCTGCGTTC 
     3961 GCTGCAGGCC TCTGACTCCG GAAGGCAAAC AGAGGCCTCA GGGGGGAGAC TTCATGAGAT 
     4021 TCCTGCCCAT GTTCCTTTCG GATAACCCTA ACCCCAAGTG TGGCAAAGGG GGACATGCTG 
     4081 CCTATAGTTC TGCAGTTAAC ATCCTCCTTG GCCATGGCAC CAGGGTCGGA GCCACGTACT 
     4141 TCATGACCTA CCACACCGTG CTGCAGACCT CTGCTGACTT TATTGACGCT CTGAAGAAAG 
     4201 CCCGACTTAT AGCCAGTAAT GTCACCGAAA CCATGGGCAT TAACGGCAGT GCCTACCGAG 
     4261 TATTTCCTTA CAGTGTGTTT TATGTCTTCT ACGAACAGTA CCTGACCATC ATTGACGACA 
     4321 CTATCTTCAA CCTCGGTGTG TCCCTGGGCG CGATATTTCT GGTGACCATG GTCCTCCTGG 
     4381 GCTGTGAGCT CTGGTCTGCA GTCATCATGT GTGCCACCAT CGCCATGGTC TTGGTCAACA 
     4441 TGTTTGGAGT TATGTGGCTC TGGGGCATCA GTCTGAACGC TGTATCCTTG GTCAACCTGG 
     4501 TGATGAGCTG TGGCATCTCC GTGGAGTTCT GCAGCCACAT AACCAGAGCG TTCACGGTGA 
     4561 GCATGAAAGG CAGCCGCGTG GAGCGCGCGG AAGAGGCACT TGCCCACATG GGCAGCTCCG 
     4621 TGTTCAGTGG AATCACACTT ACAAAATTTG GAGGGATTGT GGTGTTGGCT TTTGCCAAAT 
     4681 CTCAAATTTT CCAGATATTC TACTTCAGGA TGTATTTGGC CATGGTCTTA CTGGGAGCCA 
     4741 CTCACGGATT AATATTTCTC CCTGTCTTAC TCAGTTACAT AGGGCCATCA GTAAATAAAG 
     4801 CCAAAAGTTG TGCCACTGAA GAGCGATACA AAGGAACAGA GCGCGAACGG CTTCTAAATT 
     4861 TCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4921 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4981 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5041 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5101 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5161 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5221 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5281 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5341 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5401 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5461 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5521 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5581 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5641 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5701 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5761 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5821 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5881 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5941 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6001 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6061 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6121 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6181 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6241 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6301 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6361 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6421 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6481 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6541 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6601 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6661 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6721 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6781 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6841 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6901 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6961 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7021 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7081 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7141 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7201 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7261 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7321 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7381 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7441 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7501 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7561 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7621 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7681 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7741 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7801 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7861 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7921 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7981 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8041 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8101 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8161 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8221 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8281 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8341 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8401 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8461 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8521 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8581 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8641 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8701 TTTAACGCGA ATTTT
//