LOCUS       RC209252                8010 bp    DNA     circular  SYN 
DEFINITION  MYO1A (Myc-DDK-tagged)-Human myosin IA (MYO1A)
ACCESSION   NM_005379
VERSION     NM_005379.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4167..4258 
                     /label="Myc-DDK"  
     misc_feature    4288..4873 
                     /label="PolyA signal"  
     misc_feature    4345..4364 
                     /label="Restriction Enzyme"  
     misc_feature    5149..5821 
                     /label="ColE1"  
     misc_feature    6251..7045 
                     /label="Kan/Neo"  
     misc_feature    7080..7382 
                     /label="SV40 ori"  
     misc_feature    7468..7945 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4149..4155 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4149 
                     /label="MYO1A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTCTCCTG GAAGGTTCTG TGGGGGTGGA GGATCTTGTC CTCCTGGAAC 
     1081 CCTTGGTGGA GGAGTCACTG CTCAAGAATC TTCAGCTTCG CTATGAAAAC AAGGAGATTT 
     1141 ATACCTACAT TGGGAATGTG GTGATCTCAG TGAATCCCTA TCAACAGCTT CCCATCTATG 
     1201 GGCCAGAGTT CATTGCCAAA TATCAAGACT ATACTTTCTA TGAGCTGAAG CCCCATATCT 
     1261 ACGCATTGGC AAATGTGGCG TACCAGTCAC TGAGGGACAG GGACCGAGAC CAGTGTATCC 
     1321 TCATCACAGG CGAGAGTGGA TCAGGGAAGA CTGAGGCCAG CAAGCTGGTG ATGTCTTATG 
     1381 TGGCTGCCGT CTGTGGGAAA GGAGAGCAGG TGAACTCTGT GAAGGAGCAG CTGCTACAGT 
     1441 CTAACCCAGT GCTGGAGGCT TTTGGCAATG CCAAGACCAT TCGCAACAAC AATTCCTCCC 
     1501 GATTTGGAAA ATACATGGAT ATTGAATTTG ACTTCAAGGG ATCCCCCCTC GGTGGTGTCA 
     1561 TCACAAACTA TCTGCTTGAG AAATCCCGAT TAGTGAAGCA GCTCAAAGGA GAAAGGAACT 
     1621 TCCACATCTT CTATCAGCTG CTGGCTGGAG CAGATGAACA GCTGCTGAAG GCCCTGAAGC 
     1681 TTGAGCGGGA TACAACTGGC TATGCCTATC TGAATCATGA AGTATCCAGA GTGGATGGCA 
     1741 TGGACGACGC CTCCAGCTTC AGGGCTGTAC AGAGTGCAAT GGCAGTGATT GGGTTCTCGG 
     1801 AGGAGGAGAT TCGACAAGTG CTAGAGGTGA CATCCATGGT GCTAAAGCTG GGGAACGTGT 
     1861 TGGTGGCTGA TGAGTTCCAG GCCAGTGGGA TACCAGCAAG TGGCATCCGT GATGGGAGAG 
     1921 GTGTTCGGGA GATTGGGGAG ATGGTGGGCT TGAATTCAGA AGAAGTAGAG AGAGCTTTGT 
     1981 GCTCGAGGAC CATGGAAACA GCCAAGGAAA AGGTGGTCAC TGCACTGAAT GTTATGCAGG 
     2041 CTCAGTATGC TCGGGACGCC CTGGCTAAGA ACATCTACAG CCGCCTCTTT GACTGGATAG 
     2101 TGAATCGAAT CAATGAGAGC ATCAAGGTGG GCATCGGGGA AAAGAAGAAG GTAATGGGAG 
     2161 TCCTTGATAT CTACGGTTTT GAGATATTAG AGGATAATAG CTTTGAGCAA TTTGTGATCA 
     2221 ACTACTGCAA TGAGAAGCTG CAGCAGGTGT TCATAGAGAT GACCCTGAAA GAAGAGCAAG 
     2281 AGGAATATAA GAGAGAAGGC ATACCGTGGA CAAAGGTGGA CTACTTTGAT AATGGCATCA 
     2341 TTTGTAAGCT CATTGAGCAT AATCAGCGAG GTATCCTGGC CATGTTGGAT GAGGAGTGCC 
     2401 TGCGGCCTGG GGTGGTCAGT GACTCCACTT TCCTAGCAAA GCTGAACCAG CTCTTCTCCA 
     2461 AGCATGGCCA CTACGAGAGC AAAGTCACCC AGAATGCCCA GCGTCAGTAT GACCACACCA 
     2521 TGGGCCTCAG CTGCTTCCGC ATCTGCCACT ATGCGGGCAA GGTGACATAC AACGTGACCA 
     2581 GCTTTATTGA CAAGAATAAT GACCTACTCT TCCGAGACCT GTTGCAGGCC ATGTGGAAGG 
     2641 CCCAGCACCC CCTCCTTCGG TCCTTGTTTC CTGAGGGCAA TCCTAAGCAG GCATCTCTCA 
     2701 AACGCCCCCC GACTGCTGGG GCCCAGTTCA AGAGTTCTGT GGCCATCCTC ATGAAGAATC 
     2761 TGTATTCCAA GAGCCCCAAC TACATCAGGT GCATAAAGCC CAATGAGCAT CAGCAGCGAG 
     2821 GTCAGTTCTC TTCAGACCTG GTGGCAACCC AGGCTCGGTA CCTGGGACTG CTGGAGAACG 
     2881 TACGGGTGCG ACGGGCAGGC TATGCCCACC GCCAGGGTTA TGGGCCCTTC CTGGAAAGGT 
     2941 ACCGATTGCT GAGCCGGAGC ACCTGGCCTC ACTGGAATGG GGGAGACCGG GAAGGTGTTG 
     3001 AGAAGGTCCT GGGGGAGCTG AGCATGTCCT CGGGGGAGCT GGCCTTTGGC AAGACAAAGA 
     3061 TCTTCATTAG AAGCCCCAAG ACTCTTTTCT ACCTCGAAGA ACAGAGGCGC CTGAGACTCC 
     3121 AGCAGCTGGC CACACTCATA CAGAAGATTT ACCGAGGCTG GCGCTGCCGC ACCCACTACC 
     3181 AACTGATGCG AAAGAGTCAG ATCCTCATCT CCTCTTGGTT TCGGGGAAAC ATGCAAAAGA 
     3241 AATGCTATGG GAAGATAAAG GCATCCGTGT TATTGATCCA GGCTTTTGTG AGAGGGTGGA 
     3301 AGGCCCGAAA GAATTATCGC AAATATTTCC GGTCAGAGGC TGCCCTCACC TTGGCAGATT 
     3361 TCATCTACAA GAGCATGGTA CAGAAATTCC TACTGGGGCT GAAGAACAAT TTGCCATCCA 
     3421 CAAACGTCTT AGACAAGACA TGGCCAGCCG CCCCCTACAA GTGCCTCAGC ACAGCAAATC 
     3481 AGGAGCTGCA GCAGCTCTTC TACCAGTGGA AGTGCAAGAG GTTCCGGGAT CAGCTGTCCC 
     3541 CGAAGCAGGT AGAGATCCTG AGGGAAAAGC TCTGTGCCAG TGAACTGTTC AAGGGCAAGA 
     3601 AGGCTTCATA TCCCCAGAGT GTCCCCATTC CATTCTGTGG TGACTACATT GGGCTGCAAG 
     3661 GGAACCCCAA GCTGCAGAAG CTGAAAGGCG GGGAGGAGGG GCCTGTTCTG ATGGCAGAGG 
     3721 CCGTGAAGAA GGTCAATCGT GGCAATGGCA AGACTTCTTC TCGGATTCTC CTCCTGACCA 
     3781 AGGGCCATGT GATTCTCACA GACACCAAGA AGTCCCAGGC CAAAATTGTC ATTGGGCTAG 
     3841 ACAATGTGGC TGGGGTGTCA GTCACCAGCC TCAAGGATGG GCTCTTTAGC TTGCATCTGA 
     3901 GTGAGATGTC ATCGGTGGGC TCCAAGGGGG ACTTCCTGCT GGTCAGCGAG CATGTGATTG 
     3961 AACTGCTGAC CAAAATGTAC CGGGCTGTGC TGGATGCCAC GCAGAGGCAG CTTACAGTCA 
     4021 CCGTGACTGA GAAGTTCTCA GTGAGGTTCA AGGAGAACAG TGTGGCTGTC AAGGTCGTCC 
     4081 AGGGCCCTGC AGGTGGTGAC AACAGCAAGC TACGCTACAA AAAAAAGGGG AGTCATTGCT 
     4141 TGGAGGTGAC TGTGCAGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4201 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4261 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4321 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4381 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4441 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4501 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4561 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4621 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4681 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4741 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4801 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4861 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4921 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4981 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5041 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5101 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5161 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5221 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5281 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5341 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5401 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5461 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5521 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5581 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5641 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5701 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5761 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5821 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5881 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5941 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6001 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6061 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6121 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6181 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6241 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6301 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6361 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6421 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6481 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6541 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6601 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6661 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6721 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6781 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6841 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6901 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6961 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7021 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7081 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7141 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7201 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7261 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7321 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7381 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7441 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7501 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7561 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7621 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7681 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7741 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7801 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7861 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7921 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7981 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//