LOCUS       RC209242                7305 bp    DNA     circular  SYN 
DEFINITION  ANAPC4 (Myc-DDK-tagged)-Human anaphase promoting complex subunit 4 (ANAPC4)
ACCESSION   NM_013367
VERSION     NM_013367.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3462..3553 
                     /label="Myc-DDK"  
     misc_feature    3583..4168 
                     /label="PolyA signal"  
     misc_feature    3640..3659 
                     /label="Restriction Enzyme"  
     misc_feature    4444..5116 
                     /label="ColE1"  
     misc_feature    5546..6340 
                     /label="Kan/Neo"  
     misc_feature    6375..6677 
                     /label="SV40 ori"  
     misc_feature    6763..7240 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3444..3450 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3444 
                     /label="ANAPC4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTGCGTTTT CCGACCTGTT TCCCATCCTT CCGGGTGGTG GGAGAGAAGC 
     1081 AGCTCCCGCA GGAGATTATT TTCCTGGTCT GGTCGCCCAA GCGGGATCTC ATTGCTTTGG 
     1141 CCAACACAGC TGGCGAGGTT TTACTTCATC GACTGGCAAG TTTTCATCGA GTTTGGAGTT 
     1201 TTCCACCAAA TGAAAATACA GGAAAGGAGG TGACGTGTCT GGCATGGAGA CCAGATGGCA 
     1261 AACTTTTGGC CTTTGCTCTT GCTGATACCA AGAAAATTGT TTTGTGTGAT GTAGAAAAAC 
     1321 CTGAGAGCTT ACACTCTTTT TCTGTGGAGG CTCCAGTTTC CTGTATGCAT TGGATGGAAG 
     1381 TGACAGTAGA AAGCAGTGTT CTCACATCAT TTTATAATGC TGAGGATGAA TCAAATCTTC 
     1441 TCTTACCTAA ACTACCTACA CTGCCAAAAA ACTATAGCAA CACCTCAAAA ATATTTAGTG 
     1501 AAGAAAATTC TGATGAAATT ATTAAGCTCT TGGGAGACGT CAGGCTTAAT ATTCTCGTCC 
     1561 TTGGAGGAAG CTCTGGATTT ATTGAGCTTT ATGCTTATGG AATGTTTAAA ATTGCTCGAG 
     1621 TCACAGGGAT TGCTGGTACT TGTCTTGCAT TATGTTTATC AAGTGATTTG AAATCATTAT 
     1681 CAGTGGTCAC AGAAGTCTCT ACCAATGGTG CTTCAGAAGT TTCATACTTT CAGCTTGAAA 
     1741 CTAATCTGTT GTACTCTTTC TTACCTGAAG TAACTCGGAT GGCCAGAAAG TTTACTCATA 
     1801 TTTCAGCTCT GTTACAGTAT ATAAATTTGT CACTAACATG TATGTGTGAA GCATGGGAAG 
     1861 AAATACTAAT GCAGATGGAT TCTCGTCTCA CCAAGTTTGT GCAGGAAAAG AACACAACCA 
     1921 CATCAGTGCA AGATGAGTTC ATGCACTTGC TATTATGGGG GAAAGCAAGT GCTGAACTTC 
     1981 AGACTCTCTT GATGAATCAG TTAACAGTAA AGGGCTTGAA AAAGCTTGGC CAGTCTATAG 
     2041 AGTCATCATA CTCCAGTATA CAAAAATTGG TCATAAGTCA TTTACAGAGT GGCTCGGAGT 
     2101 CTTTATTATA CCATTTGAGT GAATTGAAAG GAATGGCTTC ATGGAAGCAA AAATATGAAC 
     2161 CTCTTGGACT AGATGCTGCA GGAATCGAAG AAGCTATAAC TGCTGTGGGT TCTTTTATAC 
     2221 TCAAGGCAAA TGAACTTCTT CAAGTTATAG ATAGTAGTAT GAAAAACTTC AAAGCATTTT 
     2281 TTCGGTGGCT TTATGTGGCA ATGTTGAGAA TGACAGAAGA CCATGTGCTT CCCGAGCTGA 
     2341 ATAAGATGAC TCAGAAAGAT ATCACATTTG TTGCTGAATT TCTTACTGAA CATTTCAATG 
     2401 AGGCTCCAGA CCTTTATAAT CGAAAAGGAA AATACTTTAA CGTTGAAAGA GTTGGTCAGT 
     2461 ACTTGAAAGA TGAAGATGAT GATCTTGTGT CACCCCCTAA CACAGAAGGA AACCAGTGGT 
     2521 ATGACTTTCT TCAAAATAGC AGCCACCTTA AAGAAAGTCC TTTGCTGTTT CCTTATTATC 
     2581 CTCGAAAATC ATTGCATTTT GTGAAAAGGC GGATGGAGAA TATTATTGAT CAGTGTTTGC 
     2641 AAAAGCCAGC AGATGTAATT GGAAAATCGA TGAATCAAGC AATCTGTATT CCATTGTATA 
     2701 GAGATACCAG AAGTGAGGAT TCTACACGTA GATTGTTCAA ATTTCCTTTT CTGTGGAATA 
     2761 ATAAAACTTC AAATCTACAT TATCTTCTTT TTACTATTCT AGAAGATTCA CTTTATAAAA 
     2821 TGTGCATCTT AAGGAGACAT ACTGATATTT CTCAATCTGT GAGTAATGGA CTAATTGCTA 
     2881 TTAAATTTGG GAGCTTTACA TATGCCACAA CAGAAAAAGT CAGAAGAAGC ATCTACAGTT 
     2941 GTTTAGATGC ACAGTTTTAT GATGATGAAA CTGTAACAGT AGTTCTTAAA GACACTGTAG 
     3001 GACGTGAAGG AAGAGATAGA CTCTTGGTCC AGCTGCCTTT GTCTTTAGTA TATAACAGTG 
     3061 AAGATTCTGC AGAATATCAG TTCACTGGGA CTTATTCTAC AAGGCTAGAT GAACAGTGTA 
     3121 GTGCTATTCC CACCCGTACC ATGCATTTCG AGAAGCACTG GAGATTACTG GAAAGTATGA 
     3181 AAGCACAGTA TGTTGCTGGG AATGGTTTTC GAAAAGTGTC CTGTGTGTTA AGCTCAAATC 
     3241 TTCGTCATGT GAGAGTATTT GAAATGGACA TAGATGATGA ATGGGAGCTC GATGGGTCTT 
     3301 CAGATGAAGA GGAGGAGGCC AGTAATAAGC CTGTAAAAAT AAAGGAAGAA GTGTTGTCGG 
     3361 AGTCAGAGGC AGAGAACCAA CAAGCTGGTG CTGCCGCTTT AGCTCCAGAG ATAGTCATTA 
     3421 AAGTGGAAAA ACTTGACCCT GAGCTAGACT CCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3481 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3541 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     3601 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     3661 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     3721 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     3781 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     3841 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     3901 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     3961 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4021 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4081 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4141 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4201 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4261 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4321 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4381 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4441 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4501 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4561 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     4621 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     4681 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     4741 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     4801 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     4861 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     4921 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     4981 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5041 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5101 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5161 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5221 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5281 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5341 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5401 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5461 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     5521 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     5581 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     5641 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     5701 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     5761 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     5821 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     5881 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     5941 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6001 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6061 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6121 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6181 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6241 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6301 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6361 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6421 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6481 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6541 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6601 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6661 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     6721 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     6781 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     6841 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     6901 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     6961 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7021 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7081 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7141 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7201 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7261 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//