LOCUS       RC209238                8439 bp    DNA     circular  SYN 
DEFINITION  AGTPBP1 (Myc-DDK-tagged)-Human ATP/GTP binding protein 1 (AGTPBP1)
ACCESSION   NM_015239
VERSION     NM_015239.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4596..4687 
                     /label="Myc-DDK"  
     misc_feature    4717..5302 
                     /label="PolyA signal"  
     misc_feature    4774..4793 
                     /label="Restriction Enzyme"  
     misc_feature    5578..6250 
                     /label="ColE1"  
     misc_feature    6680..7474 
                     /label="Kan/Neo"  
     misc_feature    7509..7811 
                     /label="SV40 ori"  
     misc_feature    7897..8374 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4578..4584 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4578 
                     /label="AGTPBP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCAAGTTA AAAGTGATAC CAGAAAAAAG CCTTACCAAT AATTCTAGGA 
     1081 TCGTAGGACT CCTGGCTCAA CTGGAGAAGA TCAATGCTGA GCCTTCAGAA TCAGACACTG 
     1141 CCCGATATGT TACATCAAAA ATTCTTCATC TGGCTCAGAG TCAAGAAAAA ACAAGGAGAG 
     1201 AAATGACAGC CAAAGGTTCT ACAGGAATGG AAATTCTGCT GTCAACATTA GAGAACACAA 
     1261 AAGATCTTCA AACTACACTT AATATCTTAA GCATTCTTGT TGAGCTGGTG TCAGCTGGTG 
     1321 GAGGTCGAAG AGTGAGTTTC TTAGTCACCA AAGGTGGTTC ACAAATATTG TTGCAGTTAC 
     1381 TTATGAATGC CAGCAAAGAA TCTCCCCCAC ATGAGGACTT AATGGTACAG ATTCATTCTA 
     1441 TTCTTGCAAA GATTGGACCA AAAGATAAAA AATTTGGAGT AAAGGCTAGA ATTAATGGGG 
     1501 CTCTGAATAT AACCCTGAAT TTGGTCAAGC AGAATTTGCA GAATCATCGC TTGGTTCTAC 
     1561 CTTGCCTTCA GCTTTTACGA GTATATTCTG CCAACTCTGT GAATTCAGTA TCCTTAGGGA 
     1621 AAAATGGAGT TGTGGAACTG ATGTTTAAAA TCATTGGACC ATTTAGTAAG AAGAATTCCA 
     1681 GTCTTATAAA GGTTGCTTTA GACACTCTTG CTGCATTGCT AAAATCAAAA ACAAATGCCA 
     1741 GGAGAGCTGT AGACAGAGGA TATGTCCAAG TGCTTTTAAC AATTTATGTA GATTGGCACC 
     1801 GCCATGATAA CCGGCATAGA AACATGCTCA TTCGGAAAGG AATTTTACAG AGTTTAAAAA 
     1861 GTGTTACAAA CATCAAGTTG GGAAGAAAAG CATTTATTGA TGCCAATGGG ATGAAAATTC 
     1921 TGTATAATAC TTCGCAATTG CCTGTTATTC CTGTGACTGG TCCTGTGGCT CAGCTCTACA 
     1981 GCTTACCTCC TGAAGTGGAT GACGTAGTAG ATGAAAGTGA TGACAACGAT GATATTGATG 
     2041 TAGAAGCTGA AAACGAAACT GAGAATGAAG ATGACCTAGA TCAAAATTTT AAGAATGATG 
     2101 ATATTGAAAC AGATATTAAC AAACTAAAAC CCCAGCAAGA ACCGGGACGA ACAATAGAAG 
     2161 ATCTAAAAAT GTATGAACAC CTTTTCCCTG AGCTTGTTGA TGATTTTCAG GACTATGATT 
     2221 TAATCTCCAA AGAACCAAAG CCTTTTGTAT TTGAGGGAAA AGTACGTGGT CCTATTGTTG 
     2281 TTCCTACGGC AGGCGAGGAA ACATCTGGGA ATTCTGGCAA TTTAAGAAAA GTTGTAATGA 
     2341 AGGAGAACAT ATCTTCTAAA GGAGATGAAG GTGAAAAGAA GTCTACCTTT ATGGATCTAG 
     2401 CAAAAGAAGA TATTAAAGAT AATGATAGAA CATTACAACA GCAGCCAGGT GATCAAAATA 
     2461 GAACTATTTC ATCAGTCCAT GGTTTAAACA ATGATATTGT AAAGGCCTTG GACCGAATTA 
     2521 CATTGCAGAA TATTCCTTCT CAAACAGCCC CAGGTTTTAC TGCAGAAATG AAGAAGGACT 
     2581 GCAGTCTTCC TCTTACTGTC CTTACCTGTG CTAAAGCATG TCCACACATG GCTACTTGTG 
     2641 GAAATGTTCT GTTTGAGGGA AGAACAGTTC AGCTAGGGAA GCTTTGCTGC ACTGGAGTTG 
     2701 AAACTGAAGA TGATGAAGAT ACTGAGTCAA ATTCATCGGT AGAACAAGCA TCGGTTGAAG 
     2761 TACCTGATGG ACCAACACTC CATGACCCAG ACCTCTATAT TGAGATTGTG AAAAATACGA 
     2821 AGTCTGTCCC AGAATATTCA GAGGTGGCTT ATCCCGATTA TTTTGGTCAC ATTCCGCCTC 
     2881 CATTCAAAGA GCCTATTTTA GAAAGGCCTT ATGGTGTACA AAGGACAAAA ATTGCTCAAG 
     2941 ATATTGAAAG GCTAATACAT CAGAGTGATA TCATAGATCG TGTGGTATAT GACTTGGATA 
     3001 ACCCAAATTA CACCATTCCA GAAGAGGGAG ATATTTTGAA ATTTAACTCC AAATTTGAGT 
     3061 CTGGGAATCT GCGCAAAGTA ATTCAAATTA GAAAAAATGA ATATGATCTT ATTCTGAACT 
     3121 CAGACATAAA CAGCAATCAT TATCATCAGT GGTTTTACTT TGAAGTCAGT GGAATGCGAC 
     3181 CAGGTGTTGC TTACAGGTTT AACATCATTA ACTGTGAAAA GTCCAACAGT CAGTTTAATT 
     3241 ATGGTATGCA ACCACTCATG TATTCGGTTC AGGAAGCATT AAATGCCAGA CCATGGTGGA 
     3301 TTCGTATGGG GACTGACATT TGTTACTATA AAAATCATTT CTCAAGAAGT TCAGTTGCTG 
     3361 CAGGTGGGCA AAAGGGAAAA TCCTACTATA CAATTACATT TACTGTCAAT TTTCCACATA 
     3421 AAGATGATGT TTGCTACTTT GCTTATCACT ATCCATATAC GTATTCAACT TTACAGATGC 
     3481 ATCTTCAAAA ATTGGAATCA GCACACAATC CTCAGCAAAT CTATTTTCGG AAAGATGTGT 
     3541 TATGTGAAAC CCTGTCTGGA AACAGCTGCC CCTTGGTGAC TATAACAGCA ATGCCAGAGT 
     3601 CTAATTATTA TGAACATATC TGCCATTTCA GAAATCGCCC TTACGTTTTC TTGTCTGCTC 
     3661 GGGTACATCC TGGAGAAACT AATGCAAGTT GGGTTATGAA AGGAACGTTG GAATATCTCA 
     3721 TGAGCAATAA CCCCACTGCT CAGAGCTTAC GAGAATCTTA TATTTTTAAA ATTGTCCCTA 
     3781 TGTTAAATCC AGATGGTGTC ATCAATGGAA ATCATCGCTG TTCTTTAAGT GGAGAGGATT 
     3841 TGAATAGGCA GTGGCAAAGT CCAAGTCCGG ATTTACATCC TACAATTTAC CATGCTAAGG 
     3901 GGCTGTTGCA ATACTTGGCT GCAGTGAAGC GTTTACCCTT GGTTTATTGT GATTATCATG 
     3961 GCCATTCCCG AAAGAAGAAT GTATTTATGT ATGGTTGCAG CATCAAAGAG ACAGTGTGGC 
     4021 ATACCAATGA TAATGCAACT TCATGTGATG TTGTGGAGGA TACGGGATAC AGGACATTGC 
     4081 CTAAGATACT GAGCCATATC GCCCCAGCAT TTTGCATGAG CAGCTGTAGC TTCGTAGTGG 
     4141 AAAAATCTAA AGAATCCACA GCACGTGTTG TAGTTTGGAG GGAAATAGGA GTACAAAGAA 
     4201 GTTATACCAT GGAGAGTACT TTATGTGGCT GTGATCAGGG AAAATACAAG GGTTTACAGA 
     4261 TTGGTACCCG AGAACTGGAA GAGATGGGAG CAAAATTTTG TGTTGGTCTT TTACGTTTGA 
     4321 AAAGACTGAC CTCTCCATTG GAGTATAATC TGCCTTCCAG CCTGCTTGAC TTTGAAAATG 
     4381 ATTTAATTGA ATCAAGCTGC AAAGTAACTA GCCCTACCAC TTATGTCTTG GATGAAGATG 
     4441 AACCTCGATT CCTTGAAGAA GTTGATTACA GTGCAGAAAG TAATGATGAG TTAGATATTG 
     4501 AGTTGGCTGA AAATGTAGGA GATTATGAAC CTTCTGCTCA AGAAGAAGTA CTTTCTGACT 
     4561 CTGAATTATC AAGAACATAC CTACCTACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4621 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4681 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4741 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4801 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4861 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4921 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4981 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5041 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5101 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5161 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5221 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5281 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5341 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5401 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5461 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5521 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5581 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5641 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5701 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5761 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5821 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5881 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5941 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6001 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6061 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6121 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6181 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6241 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6301 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6361 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6421 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6481 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6541 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6601 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6661 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6721 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6781 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6841 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6901 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6961 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7021 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7081 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7141 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7201 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7261 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7321 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7381 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7441 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7501 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7561 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7621 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7681 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7741 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7801 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7861 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7921 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7981 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8041 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8101 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8161 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8221 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8281 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8341 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8401 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//