LOCUS       RC209222                7500 bp    DNA     circular  SYN 
DEFINITION  ENPP1 (Myc-DDK-tagged)-Human ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1)
ACCESSION   NM_006208
VERSION     NM_006208.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3657..3748 
                     /label="Myc-DDK"  
     misc_feature    3778..4363 
                     /label="PolyA signal"  
     misc_feature    3835..3854 
                     /label="Restriction Enzyme"  
     misc_feature    4639..5311 
                     /label="ColE1"  
     misc_feature    5741..6535 
                     /label="Kan/Neo"  
     misc_feature    6570..6872 
                     /label="SV40 ori"  
     misc_feature    6958..7435 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3639..3645 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3639 
                     /label="ENPP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACGTGGGG GAGGAGCCGC TGGAGAAGGC GGCGCGCGCC CGCACTGCCA 
     1081 AGGACCCCAA CACCTATAAA GTACTCTCGC TGGTATTGTC AGTATGTGTG TTAACAACAA 
     1141 TACTTGGTTG TATATTTGGG TTGAAACCAA GCTGTGCCAA AGAAGTTAAA AGTTGCAAAG 
     1201 GTCGCTGTTT CGAGAGAACA TTTGGGAACT GTCGCTGTGA TGCTGCCTGT GTTGAGCTTG 
     1261 GAAACTGCTG TTTAGATTAC CAGGAGACGT GCATAGAACC AGAACATATA TGGACTTGCA 
     1321 ACAAATTCAG GTGTGGTGAG AAAAGGTTGA CCAGAAGCCT CTGTGCCTGT TCAGATGACT 
     1381 GCAAGGACAA GGGCGACTGC TGCATCAACT ACAGTTCTGT GTGTCAAGGT GAGAAAAGTT 
     1441 GGGTAGAAGA ACCATGTGAG AGCATTAATG AGCCACAGTG CCCAGCAGGG TTTGAAACGC 
     1501 CTCCTACCCT CTTATTTTCT TTGGATGGAT TCAGGGCAGA ATATTTACAC ACTTGGGGTG 
     1561 GACTTCTTCC TGTTATTAGC AAACTAAAAA AATGTGGAAC ATATACTAAA AACATGAGAC 
     1621 CGGTATATCC AACAAAAACT TTCCCCAATC ACTACAGCAT TGTCACCGGA TTGTATCCAG 
     1681 AATCTCATGG CATAATCGAC AATAAAATGT ATGATCCCAA AATGAATGCT TCCTTTTCAC 
     1741 TTAAAAGTAA AGAGAAATTT AATCCTGAGT GGTACAAAGG AGAACCAATT TGGGTCACAG 
     1801 CTAAGTATCA AGGCCTCAAG TCTGGCACAT TTTTCTGGCC AGGATCAGAT GTGGAAATTA 
     1861 ACGGAATTTT CCCAGACATC TATAAAATGT ATAATGGTTC AGTACCATTT GAAGAAAGGA 
     1921 TTTTAGCTGT TCTTCAGTGG CTACAGCTTC CTAAAGATGA AAGACCACAC TTTTACACTC 
     1981 TGTATTTAGA AGAACCAGAT TCTTCAGGTC ATTCATATGG ACCAGTCAGC AGTGAAGTCA 
     2041 TCAAAGCCTT GCAGAGGGTT GATGGTATGG TTGGTATGCT GATGGATGGT CTGAAAGAGC 
     2101 TGAACTTGCA CAGATGCCTG AACCTCATCC TTATTTCAGA TCATGGCATG GAACAAGGCA 
     2161 GTTGTAAGAA ATACATATAT CTGAATAAAT ATTTGGGGGA TGTTAAAAAT ATTAAAGTTA 
     2221 TCTATGGACC TGCAGCTCGA TTGAGACCCT CTGATGTCCC AGATAAATAC TATTCATTTA 
     2281 ACTATGAAGG CATTGCCCGA AATCTTTCTT GCCGGGAACC AAACCAGCAC TTCAAACCTT 
     2341 ACCTGAAACA TTTCTTACCT AAGCGTTTGC ACTTTGCTAA GAGTGATAGA ATTGAGCCCT 
     2401 TGACATTCTA TTTGGACCCT CAGTGGCAAC TTGCATTGAA TCCCTCAGAA AGGAAATATT 
     2461 GTGGAAGTGG ATTTCATGGC TCTGACAATG TATTTTCAAA TATGCAAGCC CTCTTTGTTG 
     2521 GCTATGGACC TGGATTCAAG CATGGCATTG AGGCTGACAC CTTTGAAAAC ATTGAAGTCT 
     2581 ATAACTTAAT GTGTGATTTA CTGAATTTGA CACCGGCTCC TAATAACGGA ACTCATGGAA 
     2641 GTCTTAACCA CCTTCTAAAG AATCCTGTTT ATACGCCAAA GCATCCCAAA GAAGTGCACC 
     2701 CCCTGGTACA GTGCCCCTTC ACAAGAAACC CCAGAGATAA CCTTGGCTGC TCATGTAACC 
     2761 CTTCGATTTT GCCGATTGAG GATTTTCAAA CACAGTTCAA TCTGACTGTG GCAGAAGAGA 
     2821 AGATTATTAA GCATGAAACT TTACCCTATG GAAGACCTAG AGTTCTCCAG AAGGAAAACA 
     2881 CCATCTGTCT TCTTTCCCAG CACCAGTTTA TGAGTGGATA CAGCCAAGAC ATCTTAATGC 
     2941 CCCTTTGGAC ATCCTATACC GTGGACAGAA ATGACAGTTT CTCTACGGAA GACTTCTCCA 
     3001 ACTGTCTGTA CCAGGACTTT AGAATTCCTC TTAGTCCTGT CCATAAATGT TCATTTTATA 
     3061 AAAATAACAC CAAAGTGAGT TACGGGTTCC TCTCCCCACC ACAACTAAAT AAAAATTCAA 
     3121 GTGGAATATA TTCTGAAGCT TTGCTTACTA CAAATATAGT GCCAATGTAC CAGAGTTTTC 
     3181 AAGTTATATG GCGCTACTTT CATGACACCC TACTGCGAAA GTATGCTGAA GAAAGAAATG 
     3241 GTGTCAATGT CGTCAGTGGT CCTGTGTTTG ACTTTGATTA TGATGGACGT TGTGATTCCT 
     3301 TAGAGAATCT GAGGCAAAAA AGAAGAGTCA TCCGTAACCA AGAAATTTTG ATTCCAACTC 
     3361 ACTTCTTTAT TGTGCTAACA AGCTGTAAAG ATACATCTCA GACGCCTTTG CACTGTGAAA 
     3421 ACCTAGACAC CTTAGCTTTC ATTTTGCCTC ACAGGACTGA TAACAGCGAG AGCTGTGTGC 
     3481 ATGGGAAGCA TGACTCCTCA TGGGTTGAAG AATTGTTAAT GTTACACAGA GCACGGATCA 
     3541 CAGATGTTGA GCACATCACT GGACTCAGCT TCTATCAACA AAGAAAAGAG CCAGTTTCAG 
     3601 ACATTTTAAA GTTGAAAACA CATTTGCCAA CCTTTAGCCA AGAAGACACG CGTACGCGGC 
     3661 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3721 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3781 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     3841 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     3901 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     3961 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4021 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4081 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4141 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4201 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4261 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4321 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4381 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4441 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4501 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4561 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4621 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4681 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4741 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     4801 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     4861 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     4921 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     4981 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5041 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5101 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5161 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5221 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5281 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5341 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5401 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5461 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5521 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5581 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5641 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5701 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     5761 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     5821 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     5881 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     5941 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6001 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6061 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6121 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6181 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6241 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6301 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6361 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6421 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6481 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6541 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6601 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6661 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6721 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     6781 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     6841 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     6901 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     6961 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7021 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7081 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7141 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7201 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7261 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7321 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7381 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7441 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//