LOCUS       RC209191                7830 bp    DNA     circular  SYN 
DEFINITION  EPHA3 (Myc-DDK-tagged)-Human EPH receptor A3 (EPHA3), transcript variant 1
ACCESSION   NM_005233
VERSION     NM_005233.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3987..4078 
                     /label="Myc-DDK"  
     misc_feature    4108..4693 
                     /label="PolyA signal"  
     misc_feature    4165..4184 
                     /label="Restriction Enzyme"  
     misc_feature    4969..5641 
                     /label="ColE1"  
     misc_feature    6071..6865 
                     /label="Kan/Neo"  
     misc_feature    6900..7202 
                     /label="SV40 ori"  
     misc_feature    7288..7765 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3969..3975 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3969 
                     /label="EPHA3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATTGTCAG CTCTCCATCC TCCTCCTTCT CAGCTGCTCT GTTCTCGACA 
     1081 GCTTCGGGGA ACTGATTCCG CAGCCTTCCA ATGAAGTCAA TCTACTGGAT TCAAAAACAA 
     1141 TTCAAGGGGA GCTGGGCTGG ATCTCTTATC CATCACATGG GTGGGAAGAG ATCAGTGGTG 
     1201 TGGATGAACA TTACACACCC ATCAGGACTT ACCAGGTGTG CAATGTCATG GACCACAGTC 
     1261 AAAACAATTG GCTGAGAACA AACTGGGTCC CCAGGAACTC AGCTCAGAAG ATTTATGTGG 
     1321 AGCTCAAGTT CACTCTACGA GACTGCAATA GCATTCCATT GGTTTTAGGA ACTTGCAAGG 
     1381 AGACATTCAA CCTGTACTAC ATGGAGTCTG ATGATGATCA TGGGGTGAAA TTTCGAGAGC 
     1441 ATCAGTTTAC AAAGATTGAC ACCATTGCAG CTGATGAAAG TTTCACTCAA ATGGATCTTG 
     1501 GGGACCGTAT TCTGAAGCTC AACACTGAGA TTAGAGAAGT AGGTCCTGTC AACAAGAAGG 
     1561 GATTTTATTT GGCATTTCAA GATGTTGGTG CTTGTGTTGC CTTGGTGTCT GTGAGAGTAT 
     1621 ACTTCAAAAA GTGCCCATTT ACAGTGAAGA ATCTGGCTAT GTTTCCAGAC ACGGTACCCA 
     1681 TGGACTCCCA GTCCCTGGTG GAGGTTAGAG GGTCTTGTGT CAACAATTCT AAGGAGGAAG 
     1741 ATCCTCCAAG GATGTACTGC AGTACAGAAG GCGAATGGCT TGTACCCATT GGCAAGTGTT 
     1801 CCTGCAATGC TGGCTATGAA GAAAGAGGTT TTATGTGCCA AGCTTGTCGA CCAGGTTTCT 
     1861 ACAAGGCATT GGATGGTAAT ATGAAGTGTG CTAAGTGCCC GCCTCACAGT TCTACTCAGG 
     1921 AAGATGGTTC AATGAACTGC AGGTGTGAGA ATAATTACTT CCGGGCAGAC AAAGACCCTC 
     1981 CATCCATGGC TTGTACCCGA CCTCCATCTT CACCAAGAAA TGTTATCTCT AATATAAACG 
     2041 AGACCTCAGT TATCCTGGAC TGGAGTTGGC CCCTGGACAC AGGAGGCCGG AAAGATGTTA 
     2101 CCTTCAACAT CATATGTAAA AAATGTGGGT GGAATATAAA ACAGTGTGAG CCATGCAGCC 
     2161 CAAATGTCCG CTTCCTCCCT CGACAGTTTG GACTCACCAA CACCACGGTG ACAGTGACAG 
     2221 ACCTTCTGGC ACATACTAAC TACACCTTTG AGATTGATGC CGTTAATGGG GTGTCAGAGC 
     2281 TGAGCTCCCC ACCAAGACAG TTTGCTGCGG TCAGCATCAC AACTAATCAG GCTGCTCCAT 
     2341 CACCTGTCCT GACGATTAAG AAAGATCGGA CCTCCAGAAA TAGCATCTCT TTGTCCTGGC 
     2401 AAGAACCTGA ACATCCTAAT GGGATCATAT TGGACTACGA GGTCAAATAC TATGAAAAGC 
     2461 AGGAACAAGA AACAAGTTAT ACCATTCTGA GGGCAAGAGG CACAAATGTT ACCATCAGTA 
     2521 GCCTCAAGCC TGACACTATA TACGTATTCC AAATCCGAGC CCGAACAGCC GCTGGATATG 
     2581 GGACGAACAG CCGCAAGTTT GAGTTTGAAA CTAGTCCAGA CTCTTTCTCC ATCTCTGGTG 
     2641 AAAGTAGCCA AGTGGTCATG ATCGCCATTT CAGCGGCAGT AGCAATTATT CTCCTCACTG 
     2701 TTGTCATCTA TGTTTTGATT GGGAGGTTCT GTGGCTATAA GTCAAAACAT GGGGCAGATG 
     2761 AAAAAAGACT TCATTTTGGC AATGGGCATT TAAAACTTCC AGGTCTCAGG ACTTATGTTG 
     2821 ACCCACATAC ATATGAAGAC CCTACCCAAA CTGTTCATGA GTTTGCCAAG GAATTGGATG 
     2881 CCACCAACAT ATCCATTGAT AAAGTTGTTG GAGCAGGTGA ATTTGGAGAG GTGTGCAGTG 
     2941 GTCGCTTAAA ACTTCCTTCA AAAAAAGAGA TTTCAGTGGC CATTAAGACC CTGAAAGTTG 
     3001 GCTACACAGA AAAGCAGAGG AGAGACTTCC TGGGAGAAGC AAGCATTATG GGACAGTTTG 
     3061 ACCACCCCAA TATCATTCGA CTGGAAGGAG TTGTTACCAA AAGTAAGCCA GTTATGATTG 
     3121 TCACAGAATA CATGGAGAAT GGTTCCTTGG ATAGTTTCCT ACGTAAACAC GATGCCCAGT 
     3181 TTACTGTCAT TCAGCTAGTG GGGATGCTTC GAGGGATAGC ATCTGGCATG AAGTACCTGT 
     3241 CAGACATGGG CTATGTTCAC CGAGACCTCG CTGCTCGGAA CATCTTGATC AACAGTAACT 
     3301 TGGTGTGTAA GGTTTCTGAT TTCGGACTTT CGCGTGTCCT GGAGGATGAC CCAGAAGCTG 
     3361 CTTATACAAC AAGAGGAGGG AAGATCCCAA TCAGGTGGAC ATCACCAGAA GCTATAGCCT 
     3421 ACCGCAAGTT CACGTCAGCC AGCGATGTAT GGAGTTATGG GATTGTTCTC TGGGAGGTGA 
     3481 TGTCTTATGG AGAGAGACCA TACTGGGAGA TGTCCAATCA GGATGTAATT AAAGCTGTAG 
     3541 ATGAGGGCTA TCGACTGCCA CCCCCCATGG ACTGCCCAGC TGCCTTGTAT CAGCTGATGC 
     3601 TGGACTGCTG GCAGAAAGAC AGGAACAACA GACCCAAGTT TGAGCAGATT GTTAGTATTC 
     3661 TGGACAAGCT TATCCGGAAT CCCGGCAGCC TGAAGATCAT CACCAGTGCA GCCGCAAGGC 
     3721 CATCAAACCT TCTTCTGGAC CAAAGCAATG TGGATATCAC TACCTTCCGC ACAACAGGTG 
     3781 ACTGGCTTAA TGGTGTCTGG ACAGCACACT GCAAGGAAAT CTTCACGGGT GTGGAGTACA 
     3841 GTTCTTGTGA CACAATAGCC AAGATTTCCA CAGATGACAT GAAAAAGGTT GGTGTCACCG 
     3901 TGGTTGGGCC ACAGAAGAAG ATCATCAGTA GCATTAAAGC TCTAGAAACG CAATCAAAGA 
     3961 ATGGCCCAGT TCCCGTGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4021 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4081 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4141 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4201 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4261 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4321 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4381 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4441 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4501 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4561 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4621 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4681 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4741 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4801 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     4861 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     4921 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     4981 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5041 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5101 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5161 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5221 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5281 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5341 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5401 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5461 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5521 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5581 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5641 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5701 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5761 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     5821 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     5881 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     5941 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6001 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6061 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6121 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6181 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6241 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6301 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6361 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6421 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6481 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6541 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6601 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6661 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6721 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6781 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     6841 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     6901 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     6961 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7021 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7081 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7141 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7201 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7261 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7321 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7381 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7441 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7501 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7561 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7621 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7681 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7741 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7801 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//