LOCUS       RC209184                6975 bp    DNA     circular  SYN 
DEFINITION  TF (Myc-DDK-tagged)-Human transferrin (TF)
ACCESSION   NM_001063
VERSION     NM_001063.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3132..3223 
                     /label="Myc-DDK"  
     misc_feature    3253..3838 
                     /label="PolyA signal"  
     misc_feature    3310..3329 
                     /label="Restriction Enzyme"  
     misc_feature    4114..4786 
                     /label="ColE1"  
     misc_feature    5216..6010 
                     /label="Kan/Neo"  
     misc_feature    6045..6347 
                     /label="SV40 ori"  
     misc_feature    6433..6910 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3114..3120 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3114 
                     /label="TF"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGCTCGCC GTGGGAGCCC TGCTGGTCTG CGCCGTCCTG GGGCTGTGTC 
     1081 TGGCTGTCCC TGATAAAACT GTGAGATGGT GTGCAGTGTC GGAGCATGAG GCCACTAAGT 
     1141 GCCAGAGTTT CCGCGACCAT ATGAAAAGCG TCATTCCATC CGATGGTCCC AGTGTTGCTT 
     1201 GTGTGAAGAA AGCCTCCTAC CTTGATTGCA TCAGGGCCAT TGCGGCAAAC GAAGCGGATG 
     1261 CTGTGACACT GGATGCAGGT TTGGTGTATG ATGCTTACCT GGCTCCCAAT AACCTGAAGC 
     1321 CTGTGGTGGC AGAGTTCTAT GGGTCAAAAG AGGATCCACA GACTTTCTAT TATGCTGTTG 
     1381 CTGTGGTGAA GAAGGATAGT GGCTTCCAGA TGAACCAGCT TCGAGGCAAG AAGTCCTGCC 
     1441 ACACGGGTCT AGGCAGGTCC GCTGGGTGGA ACATCCCCAT AGGCTTACTT TACTGTGACT 
     1501 TACCTGAGCC ACGTAAACCT CTTGAGAAAG CAGTGGCCAA TTTCTTCTCG GGCAGCTGTG 
     1561 CCCCTTGTGC GGATGGGACG GACTTCCCCC AGCTGTGTCA ACTGTGTCCA GGGTGTGGCT 
     1621 GCTCCACCCT TAACCAATAC TTCGGCTACT CAGGAGCCTT CAAGTGTCTG AAGAATGGTG 
     1681 CTGGGGATGT GGCCTTTGTC AAGCACTCGA CTATATTTGA GAACTTGGCA AACAAGGCTG 
     1741 ACAGGGACCA GTATGAGCTG CTTTGCCTGG ACAACACCCG GAAGCCGGTA GATGAATACA 
     1801 AGGACTGCCA CTTGGCCCAG GTCCCTTCTC ATACCGTCGT GGCCCGAAGT ATGGGCGGCA 
     1861 AGGAGGACTT GATCTGGGAG CTTCTCAACC AGGCCCAGGA ACATTTTGGC AAAGACAAAT 
     1921 CAAAAGAATT CCAACTATTC AGCTCTCCTC ATGGGAAGGA CCTGCTGTTT AAGGACTCTG 
     1981 CCCACGGGTT TTTAAAAGTC CCCCCCAGGA TGGATGCCAA GATGTACCTG GGCTATGAGT 
     2041 ATGTCACTGC CATCCGGAAT CTACGGGAAG GCACATGCCA AGAAGCCCCA ACAGATGAAT 
     2101 GCAAGCCTGT GAAGTGGTGT GCGCTGAGCC ACCACGAGAG GCTCAAGTGT GATGAGTGGA 
     2161 GTGTTAACAG TGTAGGGAAA ATAGAGTGTG TATCAGCAGA GACCACCGAA GACTGCATCG 
     2221 CCAAGATCAT GAATGGAGAA GCTGATGCCA TGAGCTTGGA TGGAGGGTTT GTCTACATAG 
     2281 CGGGCAAGTG TGGTCTGGTG CCTGTCTTGG CAGAAAACTA CAATAAGAGC GATAATTGTG 
     2341 AGGATACACC AGAGGCAGGG TATTTTGCTG TAGCAGTGGT GAAGAAATCA GCTTCTGACC 
     2401 TCACCTGGGA CAATCTGAAA GGCAAGAAGT CCTGCCATAC GGCAGTTGGC AGAACCGCTG 
     2461 GCTGGAACAT CCCCATGGGC CTGCTCTACA ATAAGATCAA CCACTGCAGA TTTGATGAAT 
     2521 TTTTCAGTGA AGGTTGTGCC CCTGGGTCTA AGAAAGACTC CAGTCTCTGT AAGCTGTGTA 
     2581 TGGGCTCAGG CCTAAACCTG TGTGAACCCA ACAACAAAGA GGGATACTAC GGCTACACAG 
     2641 GCGCTTTCAG GTGTCTGGTT GAGAAGGGAG ATGTGGCCTT TGTGAAACAC CAGACTGTCC 
     2701 CACAGAACAC TGGGGGAAAA AACCCTGATC CATGGGCTAA GAATCTGAAT GAAAAAGACT 
     2761 ATGAGTTGCT GTGCCTTGAT GGTACCAGGA AACCTGTGGA GGAGTATGCG AACTGCCACC 
     2821 TGGCCAGAGC CCCGAATCAC GCTGTGGTCA CACGGAAAGA TAAGGAAGCT TGCGTCCACA 
     2881 AGATATTACG TCAACAGCAG CACCTATTTG GAAGCAACGT AACTGACTGC TCGGGCAACT 
     2941 TTTGTTTGTT CCGGTCGGAA ACCAAGGACC TTCTGTTCAG AGATGACACA GTATGTTTGG 
     3001 CCAAACTTCA TGACAGAAAC ACATATGAAA AATACTTAGG AGAAGAATAT GTCAAGGCTG 
     3061 TTGGTAACCT GAGAAAATGC TCCACCTCAT CACTCCTGGA AGCCTGCACT TTCCGTAGAC 
     3121 CTACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     3181 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     3241 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     3301 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     3361 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     3421 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     3481 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     3541 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     3601 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     3661 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     3721 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     3781 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     3841 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     3901 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     3961 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4021 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     4081 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     4141 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     4201 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     4261 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     4321 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     4381 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     4441 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     4501 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     4561 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     4621 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     4681 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     4741 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     4801 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     4861 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     4921 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     4981 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     5041 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     5101 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     5161 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     5221 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     5281 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     5341 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     5401 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     5461 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     5521 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     5581 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     5641 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     5701 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     5761 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     5821 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     5881 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     5941 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6001 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     6061 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     6121 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     6181 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     6241 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     6301 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6361 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     6421 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     6481 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     6541 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     6601 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     6661 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     6721 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     6781 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     6841 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     6901 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     6961 TTTAACGCGA ATTTT
//