LOCUS       RC209183                8421 bp    DNA     circular  SYN 
DEFINITION  SREBF1 (Myc-DDK-tagged)-Human sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant 1
ACCESSION   NM_001005291
VERSION     NM_001005291.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4578..4669 
                     /label="Myc-DDK"  
     misc_feature    4699..5284 
                     /label="PolyA signal"  
     misc_feature    4756..4775 
                     /label="Restriction Enzyme"  
     misc_feature    5560..6232 
                     /label="ColE1"  
     misc_feature    6662..7456 
                     /label="Kan/Neo"  
     misc_feature    7491..7793 
                     /label="SV40 ori"  
     misc_feature    7879..8356 
                     /label="f1 ori"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    4560..4566 
                     /label="Restriction Enzyme"  
     misc_feature    1029..4560 
                     /label="SREBF1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCCATG GACGAGCCAC CCTTCAGCGA GGCGGCTTTG GAGCAGGCGC 
     1081 TGGGCGAGCC GTGCGATCTG GACGCGGCGC TGCTGACCGA CATCGAAGGT GAAGTCGGCG 
     1141 CGGGGAGGGG TAGGGCCAAC GGCCTGGACG CCCCAAGGGC GGGCGCAGAT CGCGGAGCCA 
     1201 TGGATTGCAC TTTCGAAGAC ATGCTTCAGC TTATCAACAA CCAAGACAGT GACTTCCCTG 
     1261 GCCTATTTGA CCCACCCTAT GCTGGGAGTG GGGCAGGGGG CACAGACCCT GCCAGCCCCG 
     1321 ATACCAGCTC CCCAGGCAGC TTGTCTCCAC CTCCTGCCAC ATTGAGCTCC TCTCTTGAAG 
     1381 CCTTCCTGAG CGGGCCGCAG GCAGCGCCCT CACCCCTGTC CCCTCCCCAG CCTGCACCCA 
     1441 CTCCATTGAA GATGTACCCG TCCATGCCCG CTTTCTCCCC TGGGCCTGGT ATCAAGGAAG 
     1501 AGTCAGTGCC ACTGAGCATC CTGCAGACCC CCACCCCACA GCCCCTGCCA GGGGCCCTCC 
     1561 TGCCACAGAG CTTCCCAGCC CCAGCCCCAC CGCAGTTCAG CTCCACCCCT GTGTTAGGCT 
     1621 ACCCCAGCCC TCCGGGAGGC TTCTCTACAG GAAGCCCTCC CGGGAACACC CAGCAGCCGC 
     1681 TGCCTGGCCT GCCACTGGCT TCCCCGCCAG GGGTCCCGCC CGTCTCCTTG CACACCCAGG 
     1741 TCCAGAGTGT GGTCCCCCAG CAGCTACTGA CAGTCACAGC TGCCCCCACG GCAGCCCCTG 
     1801 TAACGACCAC TGTGACCTCG CAGATCCAGC AGGTCCCGGT CCTGCTGCAG CCCCACTTCA 
     1861 TCAAGGCAGA CTCGCTGCTT CTGACAGCCA TGAAGACAGA CGGAGCCACT GTGAAGGCGG 
     1921 CAGGTCTCAG TCCCCTGGTC TCTGGCACCA CTGTGCAGAC AGGGCCTTTG CCGACCCTGG 
     1981 TGAGTGGCGG AACCATCTTG GCAACAGTCC CACTGGTCGT AGATGCGGAG AAGCTGCCTA 
     2041 TCAACCGGCT CGCAGCTGGC AGCAAGGCCC CGGCCTCTGC CCAGAGCCGT GGAGAGAAGC 
     2101 GCACAGCCCA CAACGCCATT GAGAAGCGCT ACCGCTCCTC CATCAATGAC AAAATCATTG 
     2161 AGCTCAAGGA TCTGGTGGTG GGCACTGAGG CAAAGCTGAA TAAATCTGCT GTCTTGCGCA 
     2221 AGGCCATCGA CTACATTCGC TTTCTGCAAC ACAGCAACCA GAAACTCAAG CAGGAGAACC 
     2281 TAAGTCTGCG CACTGCTGTC CACAAAAGCA AATCTCTGAA GGATCTGGTG TCGGCCTGTG 
     2341 GCAGTGGAGG GAACACAGAC GTGCTCATGG AGGGCGTGAA GACTGAGGTG GAGGACACAC 
     2401 TGACCCCACC CCCCTCGGAT GCTGGCTCAC CTTTCCAGAG CAGCCCCTTG TCCCTTGGCA 
     2461 GCAGGGGCAG TGGCAGCGGT GGCAGTGGCA GTGACTCGGA GCCTGACAGC CCAGTCTTTG 
     2521 AGGACAGCAA GGCAAAGCCA GAGCAGCGGC CGTCTCTGCA CAGCCGGGGC ATGCTGGACC 
     2581 GCTCCCGCCT GGCCCTGTGC ACGCTCGTCT TCCTCTGCCT GTCCTGCAAC CCCTTGGCCT 
     2641 CCTTGCTGGG GGCCCGGGGG CTTCCCAGCC CCTCAGATAC CACCAGCGTC TACCATAGCC 
     2701 CTGGGCGCAA CGTGCTGGGC ACCGAGAGCA GAGATGGCCC TGGCTGGGCC CAGTGGCTGC 
     2761 TGCCCCCAGT GGTCTGGCTG CTCAATGGGC TGTTGGTGCT CGTCTCCTTG GTGCTTCTCT 
     2821 TTGTCTACGG TGAGCCAGTC ACACGGCCCC ACTCAGGCCC CGCCGTGTAC TTCTGGAGGC 
     2881 ATCGCAAGCA GGCTGACCTG GACCTGGCCC GGGGAGACTT TGCCCAGGCT GCCCAGCAGC 
     2941 TGTGGCTGGC CCTGCGGGCA CTGGGCCGGC CCCTGCCCAC CTCCCACCTG GACCTGGCTT 
     3001 GTAGCCTCCT CTGGAACCTC ATCCGTCACC TGCTGCAGCG TCTCTGGGTG GGCCGCTGGC 
     3061 TGGCAGGCCG GGCAGGGGGC CTGCAGCAGG ACTGTGCTCT GCGAGTGGAT GCTAGCGCCA 
     3121 GCGCCCGAGA CGCAGCCCTG GTCTACCATA AGCTGCACCA GCTGCACACC ATGGGGAAGC 
     3181 ACACAGGCGG GCACCTCACT GCCACCAACC TGGCGCTGAG TGCCCTGAAC CTGGCAGAGT 
     3241 GTGCAGGGGA TGCCGTGTCT GTGGCGACGC TGGCCGAGAT CTATGTGGCG GCTGCATTGA 
     3301 GAGTGAAGAC CAGTCTCCCA CGGGCCTTGC ATTTTCTGAC ACGCTTCTTC CTGAGCAGTG 
     3361 CCCGCCAGGC CTGCCTGGCA CAGAGTGGCT CAGTGCCTCC TGCCATGCAG TGGCTCTGCC 
     3421 ACCCCGTGGG CCACCGTTTC TTCGTGGATG GGGACTGGTC CGTGCTCAGT ACCCCATGGG 
     3481 AGAGCCTGTA CAGCTTGGCC GGGAACCCAG TGGACCCCCT GGCCCAGGTG ACTCAGCTAT 
     3541 TCCGGGAACA TCTCTTAGAG CGAGCACTGA ACTGTGTGAC CCAGCCCAAC CCCAGCCCTG 
     3601 GGTCAGCTGA TGGGGACAAG GAATTCTCGG ATGCCCTCGG GTACCTGCAG CTGCTGAACA 
     3661 GCTGTTCTGA TGCTGCGGGG GCTCCTGCCT ACAGCTTCTC CATCAGTTCC AGCATGGCCA 
     3721 CCACCACCGG CGTAGACCCG GTGGCCAAGT GGTGGGCCTC TCTGACAGCT GTGGTGATCC 
     3781 ACTGGCTGCG GCGGGATGAG GAGGCGGCTG AGCGGCTGTG CCCGCTGGTG GAGCACCTGC 
     3841 CCCGGGTGCT GCAGGAGTCT GAGAGACCCC TGCCCAGGGC AGCTCTGCAC TCCTTCAAGG 
     3901 CTGCCCGGGC CCTGCTGGGC TGTGCCAAGG CAGAGTCTGG TCCAGCCAGC CTGACCATCT 
     3961 GTGAGAAGGC CAGTGGGTAC CTGCAGGACA GCCTGGCTAC CACACCAGCC AGCAGCTCCA 
     4021 TTGACAAGGC CGTGCAGCTG TTCCTGTGTG ACCTGCTTCT TGTGGTGCGC ACCAGCCTGT 
     4081 GGCGGCAGCA GCAGCCCCCG GCCCCGGCCC CAGCAGCCCA GGGCACCAGC AGCAGGCCCC 
     4141 AGGCTTCCGC CCTTGAGCTG CGTGGCTTCC AACGGGACCT GAGCAGCCTG AGGCGGCTGG 
     4201 CACAGAGCTT CCGGCCCGCC ATGCGGAGGG TGTTCCTACA TGAGGCCACG GCCCGGCTGA 
     4261 TGGCGGGGGC CAGCCCCACA CGGACACACC AGCTCCTCGA CCGCAGTCTG AGGCGGCGGG 
     4321 CAGGCCCCGG TGGCAAAGGA GGCGCGGTGG CGGAGCTGGA GCCGCGGCCC ACGCGGCGGG 
     4381 AGCACGCGGA GGCCTTGCTG CTGGCCTCCT GCTACCTGCC CCCCGGCTTC CTGTCGGCGC 
     4441 CCGGGCAGCG CGTGGGCATG CTGGCTGAGG CGGCGCGCAC ACTCGAGAAG CTTGGCGATC 
     4501 GCCGGCTGCT GCACGACTGT CAGCAGATGC TCATGCGCCT GGGCGGTGGG ACCACTGTCA 
     4561 CTTCCAGCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4621 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4681 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4741 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4801 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4861 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4921 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4981 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5041 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5101 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5161 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5221 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5281 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5341 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5401 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5461 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5521 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5581 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5641 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5701 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5761 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5821 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5881 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5941 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6001 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6061 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6121 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6181 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6241 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6301 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6361 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6421 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6481 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6541 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6601 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6661 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6721 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6781 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6841 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6901 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6961 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7021 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7081 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7141 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7201 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7261 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7321 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7381 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7441 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7501 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7561 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7621 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7681 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7741 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7801 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7861 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7921 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7981 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8041 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8101 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8161 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8221 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8281 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8341 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8401 CAAAAATTTA ACGCGAATTT T
//