LOCUS       RC209163L1                8946 bp    DNA     circular  SYN 
DEFINITION  AGO1 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2C, 1 (EIF2C1)
ACCESSION   NM_012199
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7237..7417 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8145..8804 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7576..8144 
                     /label="ColE1"  
     misc_feature    6112..6132 
                     /label="LR50 primer"  
     misc_feature    5909..5980 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6121..6712 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5891..5897 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5891 
                     /label="AGO1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGAAGCGGGA CCCTCGGGAG CAGCTGCGGG 
     3361 CGCTTACCTG CCCCCCCTGC AGCAGGTGTT CCAGGCACCT CGCCGGCCTG GCATTGGCAC 
     3421 TGTGGGGAAA CCAATCAAGC TCCTGGCCAA TTACTTTGAG GTGGACATCC CTAAGATCGA 
     3481 CGTGTACCAC TACGAGGTGG ACATCAAGCC GGATAAGTGT CCCCGTAGAG TCAACCGGGA 
     3541 AGTGGTGGAA TACATGGTCC AGCATTTCAA GCCTCAGATC TTTGGTGATC GCAAGCCTGT 
     3601 GTATGATGGA AAGAAGAACA TTTACACTGT CACAGCACTG CCCATTGGCA ACGAACGGGT 
     3661 CGACTTTGAG GTGACAATCC CTGGGGAAGG GAAGGATCGA ATCTTTAAGG TCTCCATCAA 
     3721 GTGGCTAGCC ATTGTGAGCT GGCGAATGCT GCATGAGGCC CTGGTCAGCG GCCAGATCCC 
     3781 TGTTCCCTTG GAGTCTGTGC AAGCCCTGGA TGTGGCCATG AGGCACCTGG CATCCATGAG 
     3841 GTACACCCCT GTGGGCCGCT CCTTCTTCTC ACCGCCTGAG GGCTACTACC ACCCGCTGGG 
     3901 GGGTGGGCGC GAGGTCTGGT TCGGCTTTCA CCAGTCTGTG CGCCCTGCCA TGTGGAAGAT 
     3961 GATGCTCAAC ATTGATGTCT CAGCCACTGC CTTTTATAAG GCACAGCCAG TGATTGAGTT 
     4021 CATGTGTGAG GTGCTGGACA TCAGGAACAT AAATGAGCAG CCCAAGCCCC TCACGGACTC 
     4081 TCAGCGCGTT CGCTTCACCA AGGAGATCAA GGGCCTGAAG GTGGAAGTCA CCCACTGTGG 
     4141 ACAGATGAAG AGGAAGTACC GCGTGTGTAA TGTTACCCGT CGCCCTGCTA GCCATCAGAC 
     4201 ATTCCCCTTA CAGCTGGAGA GTGGACAGAC TGTGGAGTGC ACAGTGGCAC AGTATTTCAA 
     4261 GCAGAAATAT AACCTTCAGC TCAAGTATCC CCATCTGCCC TGCCTACAAG TTGGCCAGGA 
     4321 ACAAAAGCAT ACCTACCTTC CCCTAGAGGT CTGTAACATT GTGGCTGGGC AGCGCTGTAT 
     4381 TAAAAAGCTG ACCGACAACC AGACCTCGAC CATGATAAAG GCCACAGCTA GATCCGCTCC 
     4441 AGACAGACAG GAGGAGATCA GTCGCCTGAT GAAGAATGCC AGCTACAACT TAGATCCCTA 
     4501 CATCCAGGAA TTTGGGATCA AAGTGAAGGA TGACATGACG GAGGTGACAG GGCGAGTGCT 
     4561 GCCGGCGCCC ATCTTGCAGT ACGGCGGCCG GAACCGGGCC ATTGCCACAC CCAATCAGGG 
     4621 TGTCTGGGAC ATGCGGGGGA AACAGTTCTA CAATGGGATT GAGATCAAAG TCTGGGCCAT 
     4681 CGCCTGCTTC GCACCCCAAA AACAGTGTCG AGAAGAGGTG CTCAAGAACT TCACAGACCA 
     4741 GCTGCGGAAG ATTTCCAAGG ATGCGGGGAT GCCTATCCAG GGTCAACCTT GTTTCTGCAA 
     4801 ATATGCACAG GGGGCAGACA GCGTGGAGCC TATGTTCCGG CATCTCAAGA ACACCTACTC 
     4861 AGGGCTGCAG CTCATTATTG TCATCCTGCC AGGGAAGACG CCGGTGTATG CTGAGGTGAA 
     4921 ACGTGTCGGA GATACACTCT TGGGAATGGC TACGCAGTGT GTGCAGGTGA AGAACGTGGT 
     4981 CAAGACCTCA CCTCAGACTC TGTCCAACCT CTGCCTCAAG ATCAATGTCA AACTTGGTGG 
     5041 CATTAACAAC ATCCTAGTCC CACACCAGCG CTCTGCCGTT TTTCAACAGC CAGTGATATT 
     5101 CCTGGGAGCA GATGTTACAC ACCCCCCAGC AGGGGATGGG AAAAAACCTT CTATCACAGC 
     5161 AGTGGTAGGC AGTATGGATG CCCACCCCAG CCGATACTGT GCTACTGTGC GGGTACAGCG 
     5221 ACCACGGCAA GAGATCATTG AAGACTTGTC CTACATGGTG CGTGAGCTCC TCATCCAATT 
     5281 CTACAAGTCC ACCCGTTTCA AGCCTACCCG CATCATCTTC TACCGAGATG GGGTGCCTGA 
     5341 AGGCCAGCTA CCCCAGATAC TCCACTATGA GCTACTGGCC ATTCGTGATG CCTGCATCAA 
     5401 ACTGGAAAAG GACTACCAGC CTGGGATCAC TTATATTGTG GTGCAGAAAC GCCATCACAC 
     5461 CCGCCTTTTC TGTGCTGACA AGAATGAGCG AATTGGGAAG AGTGGTAACA TCCCAGCTGG 
     5521 GACCACAGTG GACACCAACA TCACCCACCC ATTTGAGTTT GACTTCTATC TGTGCAGCCA 
     5581 CGCAGGCATC CAGGGCACCA GCCGACCATC CCATTACTAT GTTCTTTGGG ATGACAACCG 
     5641 TTTCACAGCA GATGAGCTCC AGATCCTGAC GTACCAGCTG TGCCACACTT ACGTACGATG 
     5701 CACACGCTCT GTCTCTATCC CAGCACCTGC CTACTATGCC CGCCTGGTGG CTTTCCGGGC 
     5761 ACGATACCAC CTGGTGGACA AGGAGCATGA CAGTGGAGAG GGGAGCCACA TATCGGGGCA 
     5821 GAGCAATGGG CGGGACCCCC AGGCCCTGGC CAAAGCCGTG CAGGTTCACC AGGATACTCT 
     5881 GCGCACCATG TACTTCGCTA CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA 
     5941 GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC 
     6001 CGGCCGCGGT CTGTACAAGT AGGATTCGTC GAGGGACCTA ATAACTTCGT ATAGCATACA 
     6061 TTATACGAAG TTATACATGT TTAAGGGTTC CGGTTCCACT AGGTACAATT CGATATCAAG 
     6121 CTTATCGATA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC 
     6181 TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT 
     6241 GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT 
     6301 GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA 
     6361 ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC 
     6421 CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG 
     6481 GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC GTCCTTTCCT 
     6541 TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT 
     6601 TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT 
     6661 CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCAT 
     6721 CGATACCGTC GACCTCGATC GAGACCTAGA AAAACATGGA GCAATCACAA GTAGCAATAC 
     6781 AGCAGCTACC AATGCTGATT GTGCCTGGCT AGAAGCACAA GAGGAGGAGG AGGTGGGTTT 
     6841 TCCAGTCACA CCTCAGGTAC CTTTAAGACC AATGACTTAC AAGGCAGCTG TAGATCTTAG 
     6901 CCACTTTTTA AAAGAAAAGG GGGGACTGGA AGGGCTAATT CACTCCCAAC GAAGACAAGA 
     6961 TATCCTTGAT CTGTGGATCT ACCACACACA AGGCTACTTC CCTGATTGGC AGAACTACAC 
     7021 ACCAGGGCCA GGGATCAGAT ATCCACTGAC CTTTGGATGG TGCTACAAGC TAGTACCAGT 
     7081 TGAGCAAGAG AAGGTAGAAG AAGCCAATGA AGGAGAGAAC ACCCGCTTGT TACACCCTGT 
     7141 GAGCCTGCAT GGGATGGATG ACCCGGAGAG AGAAGTATTA GAGTGGAGGT TTGACAGCCG 
     7201 CCTAGCATTT CATCACATGG CCCGAGAGCT GCATCCGGAC TGTACTGGGT CTCTCTGGTT 
     7261 AGACCAGATC TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA 
     7321 ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA 
     7381 CTAGAGATCC CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGCA TGTGAGCAAA 
     7441 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     7501 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     7561 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     7621 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     7681 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     7741 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     7801 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     7861 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     7921 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     7981 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     8041 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     8101 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGATTACGCC 
     8161 CCGCCCTGCC ACTCATCGCA GTACTGTTGT AATTCATTAA GCATTCTGCC GACATGGAAG 
     8221 CCATCACAAA CGGCATGATG AACCTGAATC GCCAGCGGCA TCAGCACCTT GTCGCCTTGC 
     8281 GTATAATATT TGCCCATGGT GAAAACGGGG GCGAAGAAGT TGTCCATATT GGCCACGTTT 
     8341 AAATCAAAAC TGGTGAAACT CACCCAGGGA TTGGCTGAGA CGAAAAACAT ATTCTCAATA 
     8401 AACCCTTTAG GGAAATAGGC CAGGTTTTCA CCGTAACACG CCACATCTTG CGAATATATG 
     8461 TGTAGAAACT GCCGGAAATC GTCGTGGTAT TCACTCCAGA GCGATGAAAA CGTTTCAGTT 
     8521 TGCTCATGGA AAACGGTGTA ACAAGGGTGA ACACTATCCC ATATCACCAG CTCACCGTCT 
     8581 TTCATTGCCA TACGGAACTC CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG 
     8641 GCCGGATAAA ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC 
     8701 TGAACGGTCT GGTTATAGGT ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA 
     8761 CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATACTCTTC 
     8821 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8881 GAATGTATTT AGAAAAATAA ACAAATAGGG GTCCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8941 CCTGAC
//