LOCUS       RC209163                7452 bp    DNA     circular  SYN 
DEFINITION  AGO1 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2C, 1 (EIF2C1)
ACCESSION   NM_012199
VERSION     NM_012199.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3609..3700 
                     /label="Myc-DDK"  
     misc_feature    3730..4315 
                     /label="PolyA signal"  
     misc_feature    3787..3806 
                     /label="Restriction Enzyme"  
     misc_feature    4591..5263 
                     /label="ColE1"  
     misc_feature    5693..6487 
                     /label="Kan/Neo"  
     misc_feature    6522..6824 
                     /label="SV40 ori"  
     misc_feature    6910..7387 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3591..3597 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3591 
                     /label="AGO1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAGCGGGA CCCTCGGGAG CAGCTGCGGG CGCTTACCTG CCCCCCCTGC 
     1081 AGCAGGTGTT CCAGGCACCT CGCCGGCCTG GCATTGGCAC TGTGGGGAAA CCAATCAAGC 
     1141 TCCTGGCCAA TTACTTTGAG GTGGACATCC CTAAGATCGA CGTGTACCAC TACGAGGTGG 
     1201 ACATCAAGCC GGATAAGTGT CCCCGTAGAG TCAACCGGGA AGTGGTGGAA TACATGGTCC 
     1261 AGCATTTCAA GCCTCAGATC TTTGGTGATC GCAAGCCTGT GTATGATGGA AAGAAGAACA 
     1321 TTTACACTGT CACAGCACTG CCCATTGGCA ACGAACGGGT CGACTTTGAG GTGACAATCC 
     1381 CTGGGGAAGG GAAGGATCGA ATCTTTAAGG TCTCCATCAA GTGGCTAGCC ATTGTGAGCT 
     1441 GGCGAATGCT GCATGAGGCC CTGGTCAGCG GCCAGATCCC TGTTCCCTTG GAGTCTGTGC 
     1501 AAGCCCTGGA TGTGGCCATG AGGCACCTGG CATCCATGAG GTACACCCCT GTGGGCCGCT 
     1561 CCTTCTTCTC ACCGCCTGAG GGCTACTACC ACCCGCTGGG GGGTGGGCGC GAGGTCTGGT 
     1621 TCGGCTTTCA CCAGTCTGTG CGCCCTGCCA TGTGGAAGAT GATGCTCAAC ATTGATGTCT 
     1681 CAGCCACTGC CTTTTATAAG GCACAGCCAG TGATTGAGTT CATGTGTGAG GTGCTGGACA 
     1741 TCAGGAACAT AAATGAGCAG CCCAAGCCCC TCACGGACTC TCAGCGCGTT CGCTTCACCA 
     1801 AGGAGATCAA GGGCCTGAAG GTGGAAGTCA CCCACTGTGG ACAGATGAAG AGGAAGTACC 
     1861 GCGTGTGTAA TGTTACCCGT CGCCCTGCTA GCCATCAGAC ATTCCCCTTA CAGCTGGAGA 
     1921 GTGGACAGAC TGTGGAGTGC ACAGTGGCAC AGTATTTCAA GCAGAAATAT AACCTTCAGC 
     1981 TCAAGTATCC CCATCTGCCC TGCCTACAAG TTGGCCAGGA ACAAAAGCAT ACCTACCTTC 
     2041 CCCTAGAGGT CTGTAACATT GTGGCTGGGC AGCGCTGTAT TAAAAAGCTG ACCGACAACC 
     2101 AGACCTCGAC CATGATAAAG GCCACAGCTA GATCCGCTCC AGACAGACAG GAGGAGATCA 
     2161 GTCGCCTGAT GAAGAATGCC AGCTACAACT TAGATCCCTA CATCCAGGAA TTTGGGATCA 
     2221 AAGTGAAGGA TGACATGACG GAGGTGACAG GGCGAGTGCT GCCGGCGCCC ATCTTGCAGT 
     2281 ACGGCGGCCG GAACCGGGCC ATTGCCACAC CCAATCAGGG TGTCTGGGAC ATGCGGGGGA 
     2341 AACAGTTCTA CAATGGGATT GAGATCAAAG TCTGGGCCAT CGCCTGCTTC GCACCCCAAA 
     2401 AACAGTGTCG AGAAGAGGTG CTCAAGAACT TCACAGACCA GCTGCGGAAG ATTTCCAAGG 
     2461 ATGCGGGGAT GCCTATCCAG GGTCAACCTT GTTTCTGCAA ATATGCACAG GGGGCAGACA 
     2521 GCGTGGAGCC TATGTTCCGG CATCTCAAGA ACACCTACTC AGGGCTGCAG CTCATTATTG 
     2581 TCATCCTGCC AGGGAAGACG CCGGTGTATG CTGAGGTGAA ACGTGTCGGA GATACACTCT 
     2641 TGGGAATGGC TACGCAGTGT GTGCAGGTGA AGAACGTGGT CAAGACCTCA CCTCAGACTC 
     2701 TGTCCAACCT CTGCCTCAAG ATCAATGTCA AACTTGGTGG CATTAACAAC ATCCTAGTCC 
     2761 CACACCAGCG CTCTGCCGTT TTTCAACAGC CAGTGATATT CCTGGGAGCA GATGTTACAC 
     2821 ACCCCCCAGC AGGGGATGGG AAAAAACCTT CTATCACAGC AGTGGTAGGC AGTATGGATG 
     2881 CCCACCCCAG CCGATACTGT GCTACTGTGC GGGTACAGCG ACCACGGCAA GAGATCATTG 
     2941 AAGACTTGTC CTACATGGTG CGTGAGCTCC TCATCCAATT CTACAAGTCC ACCCGTTTCA 
     3001 AGCCTACCCG CATCATCTTC TACCGAGATG GGGTGCCTGA AGGCCAGCTA CCCCAGATAC 
     3061 TCCACTATGA GCTACTGGCC ATTCGTGATG CCTGCATCAA ACTGGAAAAG GACTACCAGC 
     3121 CTGGGATCAC TTATATTGTG GTGCAGAAAC GCCATCACAC CCGCCTTTTC TGTGCTGACA 
     3181 AGAATGAGCG AATTGGGAAG AGTGGTAACA TCCCAGCTGG GACCACAGTG GACACCAACA 
     3241 TCACCCACCC ATTTGAGTTT GACTTCTATC TGTGCAGCCA CGCAGGCATC CAGGGCACCA 
     3301 GCCGACCATC CCATTACTAT GTTCTTTGGG ATGACAACCG TTTCACAGCA GATGAGCTCC 
     3361 AGATCCTGAC GTACCAGCTG TGCCACACTT ACGTACGATG CACACGCTCT GTCTCTATCC 
     3421 CAGCACCTGC CTACTATGCC CGCCTGGTGG CTTTCCGGGC ACGATACCAC CTGGTGGACA 
     3481 AGGAGCATGA CAGTGGAGAG GGGAGCCACA TATCGGGGCA GAGCAATGGG CGGGACCCCC 
     3541 AGGCCCTGGC CAAAGCCGTG CAGGTTCACC AGGATACTCT GCGCACCATG TACTTCGCTA 
     3601 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     3661 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     3721 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     3781 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     3841 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     3901 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     3961 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4021 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4081 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4141 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4201 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4261 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4321 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4381 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     4441 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     4501 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     4561 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     4621 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     4681 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     4741 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     4801 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     4861 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     4921 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     4981 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5041 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5101 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5161 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     5221 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5281 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5341 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     5401 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     5461 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     5521 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     5581 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     5641 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     5701 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     5761 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     5821 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     5881 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     5941 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6001 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6061 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6121 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6181 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6241 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6301 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6361 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     6421 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     6481 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     6541 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     6601 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     6661 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     6721 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     6781 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6841 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     6901 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     6961 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7021 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7081 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7141 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7201 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7261 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7321 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7381 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     7441 AACGCGAATT TT
//